##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937372_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1643655 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20496758748034 33.0 31.0 34.0 31.0 34.0 2 32.41246611971491 34.0 31.0 34.0 31.0 34.0 3 32.47347101429436 34.0 31.0 34.0 31.0 34.0 4 36.107685615290315 37.0 35.0 37.0 35.0 37.0 5 36.02654997551189 37.0 35.0 37.0 35.0 37.0 6 36.06534278787215 37.0 35.0 37.0 35.0 37.0 7 36.00833569088404 37.0 35.0 37.0 35.0 37.0 8 35.97836589795304 37.0 35.0 37.0 35.0 37.0 9 37.69402277241878 39.0 37.0 39.0 35.0 39.0 10-11 37.603641275085096 39.0 37.0 39.0 35.0 39.0 12-13 37.509666566280636 39.0 37.0 39.0 35.0 39.0 14-15 38.7162245118349 40.0 38.0 41.0 34.5 41.0 16-17 38.685731798948076 40.0 38.0 41.0 34.5 41.0 18-19 38.66773471318494 40.0 38.0 41.0 34.0 41.0 20-21 38.56298614976987 40.0 38.0 41.0 34.0 41.0 22-23 38.411991263373395 40.0 38.0 41.0 34.0 41.0 24-25 38.35772561760223 40.0 38.0 41.0 34.0 41.0 26-27 38.31400141757243 40.0 38.0 41.0 34.0 41.0 28-29 38.19622822307601 40.0 38.0 41.0 34.0 41.0 30-31 38.28969948073045 40.0 38.0 41.0 34.0 41.0 32-33 38.24843169643265 40.0 38.0 41.0 34.0 41.0 34-35 38.131095029066316 40.0 38.0 41.0 33.5 41.0 36-37 37.97756554751453 40.0 37.0 41.0 33.0 41.0 38-39 37.86128202086204 40.0 37.0 41.0 33.0 41.0 40-41 37.70385847394982 40.0 37.0 41.0 33.0 41.0 42-43 37.51324182994607 40.0 37.0 41.0 33.0 41.0 44-45 37.25461091287406 40.0 37.0 41.0 32.0 41.0 46-47 36.948484018848234 40.0 36.0 41.0 31.0 41.0 48-49 36.6596758443834 39.0 36.0 41.0 31.0 41.0 50-51 36.425356902756356 39.0 36.0 41.0 30.0 41.0 52-53 36.1969704712972 39.0 35.0 40.5 30.0 41.0 54-55 35.97774320036747 39.0 35.0 40.0 29.0 41.0 56-57 35.70457456096322 38.5 35.0 40.0 28.5 41.0 58-59 35.430372553851015 38.0 35.0 40.0 28.0 41.0 60-61 35.08893867630373 38.0 34.0 40.0 28.0 41.0 62-63 34.72598112134237 37.5 34.0 40.0 26.5 41.0 64-65 34.33893213600177 37.0 34.0 39.5 26.0 41.0 66-67 33.90771755629983 36.0 33.0 39.0 26.0 40.5 68-69 33.46382117901871 36.0 33.0 39.0 25.5 40.0 70-71 33.04292962939303 35.0 32.0 38.5 24.5 40.0 72-73 32.5669650869556 35.0 32.0 37.5 23.5 40.0 74-75 32.086645920220484 35.0 31.0 37.0 22.0 39.0 76-77 30.83585332688429 33.5 30.0 35.5 21.5 38.5 78-79 31.444304918002864 34.0 31.0 36.0 22.0 38.5 80-81 31.24258983789177 34.0 31.0 36.0 21.0 37.5 82-83 30.893736215933394 34.0 31.0 35.0 20.0 37.0 84-85 30.521639881848685 34.0 31.0 35.0 18.5 36.5 86-87 30.119155783908425 34.0 30.0 35.0 17.5 36.0 88-89 29.64708500263133 34.0 29.5 35.0 9.5 36.0 90-91 29.124383766666362 34.0 29.0 35.0 4.5 35.0 92-93 28.755715159203117 34.0 29.0 35.0 2.0 35.0 94-95 28.297887026170333 33.5 29.0 35.0 2.0 35.0 96-97 27.792339633317212 33.0 28.0 35.0 2.0 35.0 98-99 27.025949180332855 33.0 26.5 35.0 2.0 35.0 100-101 24.513572191244513 30.5 21.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.09668224234413003 1101 2 -0.06126731855838585 1101 3 -0.09710815096296699 1101 4 -0.026051145062005787 1101 5 -0.05872400853045434 1101 6 -0.05269307886471353 1101 7 -0.13221050708707338 1101 8 -0.08037798329256418 1101 9 -0.09390365776550169 1101 10-11 -0.08431921727960656 1101 12-13 -0.12573847854616105 1101 14-15 -0.1725261311943953 1101 16-17 -0.21825975217214477 1101 18-19 -0.0935706474383835 1101 20-21 -0.08862299047671485 1101 22-23 -0.058695741334467755 1101 24-25 -0.11136115212601538 1101 26-27 -0.11035644216282492 1101 28-29 -0.10949420507617447 1101 30-31 -0.2116025194910236 1101 32-33 -0.07557905723648162 1101 34-35 -0.10455344569545844 1101 36-37 -0.08796214784614875 1101 38-39 -0.21481529872995253 1101 40-41 -0.06737090058324924 1101 42-43 -0.0862562172701189 1101 44-45 -0.13671701373737477 1101 46-47 -0.15089387176045932 1101 48-49 -0.13439668533420246 1101 50-51 -0.07577348575193099 1101 52-53 -0.09273043239699774 1101 54-55 -0.07295329491869751 1101 56-57 -0.009973186585177984 1101 58-59 0.05649515091759838 1101 60-61 -0.04158584636198981 1101 62-63 -0.10539632002669919 1101 64-65 -0.18389431465433148 1101 66-67 -0.10390232163540247 1101 68-69 0.02592284987245108 1101 70-71 0.04272003875770736 1101 72-73 0.15921856339851814 1101 74-75 -0.06539308238978592 1101 76-77 -0.011108453437632448 1101 78-79 -0.021051691441186904 1101 80-81 -0.281443963650748 1101 82-83 -0.20082048193488689 1101 84-85 -0.22303504206099944 1101 86-87 -0.21578030261710524 1101 88-89 -0.11260925373568753 1101 90-91 -0.30743182709133166 1101 92-93 -0.24887687511673207 1101 94-95 -0.19702486140243636 1101 96-97 -0.48602947017081277 1101 98-99 -0.15974043175736696 1101 100-101 0.5893382620889938 1102 1 -0.14267321190506266 1102 2 -0.15881993080008527 1102 3 -0.12843264930282317 1102 4 -0.057621213374027036 1102 5 -0.0491761438170073 1102 6 -0.09945035935132296 1102 7 -0.19647737652459796 1102 8 -0.13578657312653064 1102 9 -0.20432607837500427 1102 10-11 -0.24315527244547042 1102 12-13 -0.21149710149774137 1102 14-15 -0.256139509808591 1102 16-17 -0.2669661999113302 1102 18-19 -0.32744585289898964 1102 20-21 -0.16431975679142852 1102 22-23 -0.21296353195540263 1102 24-25 -0.24819063302614808 1102 26-27 -0.28699655711893257 1102 28-29 -0.29511680547036434 1102 30-31 -0.29539528022085193 1102 32-33 -0.3092806823792458 1102 34-35 -0.39939583689813674 1102 36-37 -0.33322805844778003 1102 38-39 -0.25941703372799196 1102 40-41 -0.11615348211804388 1102 42-43 -0.24586817408864903 1102 44-45 -0.21749414205586248 1102 46-47 -0.17986260338562943 1102 48-49 -0.12517372129753568 1102 50-51 -0.2830833247248066 1102 52-53 -0.29209393965718533 1102 54-55 -0.09453503550066245 1102 56-57 -0.1893828903643282 1102 58-59 -0.24526452251459574 1102 60-61 -0.1557471112391582 1102 62-63 -0.11632595984873007 1102 64-65 -0.3370748010060183 1102 66-67 -0.275359536691937 1102 68-69 -0.2112005335276379 1102 70-71 -0.2110864734169482 1102 72-73 -0.1974130927732105 1102 74-75 -0.16761306335515513 1102 76-77 -0.22324455935146403 1102 78-79 -0.21720372601326687 1102 80-81 -0.19658319850965356 1102 82-83 -0.15975464668558104 1102 84-85 -0.1317788012802339 1102 86-87 -0.23278442473523953 1102 88-89 -0.09266800108255069 1102 90-91 -0.13599367508470195 1102 92-93 -0.14664102801044265 1102 94-95 -0.10983722013162378 1102 96-97 -0.05227534467816142 1102 98-99 0.2616742170479718 1102 100-101 0.9543318481423242 1103 1 -0.024241622659680218 1103 2 0.038936021585115554 1103 3 -0.039077204373469954 1103 4 -0.09752922212861392 1103 5 0.006628292923423373 1103 6 -0.008742031997087452 1103 7 -0.06135024246263754 1103 8 -0.0906402797745045 1103 9 -0.09575637137577786 1103 10-11 0.008691227634606946 1103 12-13 -0.029439225734947172 1103 14-15 -0.07616331197220205 1103 16-17 -0.15207602136178622 1103 18-19 -0.05908942775455017 1103 20-21 -0.2454217860198824 1103 22-23 -0.03367638795563721 1103 24-25 -0.003673138483485161 1103 26-27 -0.06416730277965854 1103 28-29 -0.01310376401695379 1103 30-31 -0.18869557323829156 1103 32-33 -0.051465555368935156 1103 34-35 0.12137834501255185 1103 36-37 -0.05369768118039531 1103 38-39 0.035409957954108506 1103 40-41 -0.011938819061917627 1103 42-43 0.14804728182559046 1103 44-45 -0.07486713709480597 1103 46-47 0.23577988250072224 1103 48-49 0.15116244940029588 1103 50-51 0.1754264961069154 1103 52-53 0.23119217724661212 1103 54-55 0.00597556160238355 1103 56-57 -0.10835915459547607 1103 58-59 -0.049567878495921036 1103 60-61 0.048640582742400795 1103 62-63 0.012493077313060041 1103 64-65 -0.04632478059973977 1103 66-67 0.19690744448121222 1103 68-69 0.15131995901199957 1103 70-71 0.22001230746583644 1103 72-73 0.30738113226313857 1103 74-75 0.4993521417463427 1103 76-77 0.5061261254354967 1103 78-79 0.42111939942356 1103 80-81 0.29591466983787384 1103 82-83 0.40075417989060114 1103 84-85 0.22014756288810844 1103 86-87 0.09720442142144137 1103 88-89 0.3496967088901535 1103 90-91 0.3483138406828594 1103 92-93 0.3800487126192671 1103 94-95 0.4994140445219273 1103 96-97 0.5903338122297832 1103 98-99 0.6895057333305097 1103 100-101 1.3092032600589718 1104 1 0.012521390867341609 1104 2 0.054396822950927515 1104 3 0.06611626140401938 1104 4 -0.0018517204461119263 1104 5 0.03577780681898446 1104 6 0.016016955180944592 1104 7 0.030803475500647437 1104 8 -0.01435014374683874 1104 9 0.020055060308848738 1104 10-11 0.0032180005246402743 1104 12-13 0.027273808040355618 1104 14-15 -0.06062931088257528 1104 16-17 -0.05435693932273722 1104 18-19 -0.08058210486874628 1104 20-21 0.001886940552573435 1104 22-23 -0.13465303979298682 1104 24-25 -0.010262206159360687 1104 26-27 -0.14191305719567282 1104 28-29 -0.16982473686677224 1104 30-31 -0.18604472353881363 1104 32-33 -0.07801156266997111 1104 34-35 -0.054022031984231944 1104 36-37 -0.13440274383353312 1104 38-39 -0.06875033560228161 1104 40-41 0.041631725077870385 1104 42-43 -0.0624980670421067 1104 44-45 0.027214065798794707 1104 46-47 -0.05588168567771845 1104 48-49 -0.045411585018875655 1104 50-51 -0.11169887426184033 1104 52-53 -0.07934144509817287 1104 54-55 -0.09196421214127781 1104 56-57 -0.042181505785165996 1104 58-59 -0.025624285256220958 1104 60-61 -0.06731324243480685 1104 62-63 -0.07570530632049355 1104 64-65 0.02879051806970523 1104 66-67 -0.03569323609412578 1104 68-69 0.09647807041542222 1104 70-71 0.07662278879225681 1104 72-73 0.3244027993813887 1104 74-75 0.2420511066075619 1104 76-77 0.20728625001341427 1104 78-79 -0.008100837787246462 1104 80-81 -0.03982660664877358 1104 82-83 -0.02165993971702207 1104 84-85 0.029694263583543545 1104 86-87 -0.09377657141445184 1104 88-89 0.07258622610702403 1104 90-91 0.037891292545850774 1104 92-93 0.021069983512145285 1104 94-95 0.3130380790867626 1104 96-97 0.13091923521493243 1104 98-99 0.30703207794752885 1104 100-101 0.9303193045644029 1105 1 0.025806733648025215 1105 2 0.07053081960917496 1105 3 0.025870780933161086 1105 4 0.02098462171898774 1105 5 -0.005808770901836624 1105 6 -0.01790620968294121 1105 7 -0.034709282197312064 1105 8 -0.04314133459094194 1105 9 -0.03265916976941696 1105 10-11 -0.016950280483918334 1105 12-13 0.01193913046902395 1105 14-15 0.020761472921236646 1105 16-17 -0.04236558264739898 1105 18-19 -0.007093430387918431 1105 20-21 0.12989734269330455 1105 22-23 -0.07216952869359972 1105 24-25 -0.06408040739304255 1105 26-27 0.06921239664168155 1105 28-29 -0.06049861467931095 1105 30-31 -0.022234562607145847 1105 32-33 -0.0892978225476071 1105 34-35 -0.06045741433828056 1105 36-37 -0.07007875735128977 1105 38-39 -0.10971975732868344 1105 40-41 -0.1553179853456257 1105 42-43 -0.133047006099126 1105 44-45 -0.18350780256638188 1105 46-47 -0.1668174386394412 1105 48-49 0.03586299129489845 1105 50-51 -0.012079218236010547 1105 52-53 -0.11110377879582956 1105 54-55 0.027732643346858765 1105 56-57 -0.0082583409212873 1105 58-59 0.08981215238746643 1105 60-61 -0.04599544949770262 1105 62-63 -0.015734389600439158 1105 64-65 -0.11530048809871829 1105 66-67 -0.1796005087985577 1105 68-69 0.12464896452213026 1105 70-71 0.2279233704578516 1105 72-73 0.18420631450091918 1105 74-75 0.1727254869389725 1105 76-77 0.2615520071209154 1105 78-79 0.17591058195420928 1105 80-81 0.13746833424244542 1105 82-83 -0.020026753370459005 1105 84-85 -0.06967884411881542 1105 86-87 -0.0016695725828093089 1105 88-89 0.22105071686695865 1105 90-91 0.3655226070095985 1105 92-93 0.49708098867552764 1105 94-95 0.7289917970982955 1105 96-97 0.6977039938174272 1105 98-99 0.7405535417850153 1105 100-101 1.132454381638233 1106 1 -0.1566305858966075 1106 2 -0.018309827030272174 1106 3 -0.005386614778508658 1106 4 -0.032091290743927914 1106 5 -0.13412630326387642 1106 6 -0.02640918331870523 1106 7 -0.040640435077627046 1106 8 -0.10315517616504621 1106 9 -0.08106399871340386 1106 10-11 -0.21273013549278375 1106 12-13 -0.1542211631463246 1106 14-15 -0.12945440190590318 1106 16-17 -0.040984879234471805 1106 18-19 -0.09017895008017973 1106 20-21 -0.043179260569068845 1106 22-23 -0.10566166061583004 1106 24-25 -0.2083841902875676 1106 26-27 -0.13797639500936754 1106 28-29 -0.2695520531335518 1106 30-31 -0.14376831647530253 1106 32-33 -0.23021144319381648 1106 34-35 -0.1073575797463775 1106 36-37 -0.15007793862601915 1106 38-39 -0.21846310358679233 1106 40-41 -0.0739295296277831 1106 42-43 -0.2013647687276432 1106 44-45 -0.08236951858896191 1106 46-47 -0.16174850580944877 1106 48-49 -0.23354572275634666 1106 50-51 -0.039920610683012114 1106 52-53 0.11780425916744264 1106 54-55 0.09228785165486642 1106 56-57 -0.07934966332889815 1106 58-59 -0.14518451514483388 1106 60-61 0.11645512316223972 1106 62-63 0.24956995232994217 1106 64-65 0.21411451990304187 1106 66-67 0.2734357466590751 1106 68-69 0.2645584953797666 1106 70-71 0.34184042079538557 1106 72-73 0.27549482177286677 1106 74-75 0.30012604699822276 1106 76-77 0.5164859142442104 1106 78-79 0.36851672520810297 1106 80-81 0.21106457219982744 1106 82-83 0.15591922611279685 1106 84-85 0.07963272306206903 1106 86-87 0.11715279345646579 1106 88-89 0.35244921476193625 1106 90-91 0.3149311419646921 1106 92-93 0.5291299893829589 1106 94-95 0.4311680712075159 1106 96-97 0.7433607202211903 1106 98-99 0.631339940199755 1106 100-101 1.175749341488718 1107 1 -0.02514818168185684 1107 2 -0.03750208847235115 1107 3 -0.00787533713249644 1107 4 0.013283711747448024 1107 5 0.017078528019887074 1107 6 -0.0649013338113349 1107 7 -0.15064780956191015 1107 8 -0.058700987657282155 1107 9 -0.09217223089671478 1107 10-11 0.015462982983954987 1107 12-13 -0.0257197779391376 1107 14-15 -0.0056467762905327845 1107 16-17 -0.03663858408236109 1107 18-19 -0.026477357590195538 1107 20-21 0.03189431818179145 1107 22-23 0.020687836789811342 1107 24-25 0.022546788525737327 1107 26-27 -0.09455131246164683 1107 28-29 -0.03512051957300599 1107 30-31 0.12696361769912556 1107 32-33 0.034602301250409084 1107 34-35 0.11518841112521727 1107 36-37 0.10979490134766934 1107 38-39 0.0032391399837976564 1107 40-41 0.0311163449794023 1107 42-43 0.013598790690103613 1107 44-45 0.20780713684988683 1107 46-47 0.2687200729081809 1107 48-49 0.29958630708706124 1107 50-51 0.320777374288987 1107 52-53 0.20052139122386592 1107 54-55 0.3247129939957034 1107 56-57 0.47285304862109 1107 58-59 0.43402874032457106 1107 60-61 0.4705765297620772 1107 62-63 0.38752662225200396 1107 64-65 0.5052498582988605 1107 66-67 0.4370031368224403 1107 68-69 0.673291612129141 1107 70-71 0.6113778213893326 1107 72-73 0.7233804707607021 1107 74-75 0.8676763230431135 1107 76-77 0.8488539209410426 1107 78-79 0.677307641969449 1107 80-81 0.8191040888057053 1107 82-83 0.5860323679752888 1107 84-85 0.6627610988817381 1107 86-87 0.7642232629652987 1107 88-89 0.877655334919524 1107 90-91 0.8216917502599905 1107 92-93 1.0545041946327949 1107 94-95 1.0040704954861788 1107 96-97 1.2115853297389272 1107 98-99 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2316 2 -0.2529503170885192 2316 3 -0.06203955713640852 2316 4 -0.012699846678856375 2316 5 0.039377412341671914 2316 6 0.10354161001328777 2316 7 0.10683897845922985 2316 8 0.21735597065158174 2316 9 0.17853632263626196 2316 10-11 0.18391326679682862 2316 12-13 0.0995371216498242 2316 14-15 0.08503143862432694 2316 16-17 0.0696322125510278 2316 18-19 0.11867825016083344 2316 20-21 0.03990469203186109 2316 22-23 0.025258202810562125 2316 24-25 0.06431866169074141 2316 26-27 0.22209088757750095 2316 28-29 0.19629952348825697 2316 30-31 0.13791977350260964 2316 32-33 0.04043025192569161 2316 34-35 -0.2980309077238772 2316 36-37 -0.11245994304457696 2316 38-39 -0.0663341620322555 2316 40-41 -0.3140881960266597 2316 42-43 -0.33771996053322795 2316 44-45 0.05638073246546327 2316 46-47 -0.08144874682169956 2316 48-49 -0.550687719141159 2316 50-51 -0.42333595512968714 2316 52-53 -0.32948937409225465 2316 54-55 -0.4620579005041989 2316 56-57 -0.5960523144636625 2316 58-59 -0.37037280596628364 2316 60-61 -0.47041975131054414 2316 62-63 -0.23623291973272842 2316 64-65 -0.529074373449042 2316 66-67 -0.1397119737667083 2316 68-69 -0.2662684779080493 2316 70-71 -0.1749551204779607 2316 72-73 -0.27779148069750903 2316 74-75 -0.28726348905318844 2316 76-77 -0.4076052687242573 2316 78-79 -0.4221540922251137 2316 80-81 -0.4333670405738239 2316 82-83 -0.4623650679221498 2316 84-85 -0.19496056876359802 2316 86-87 -0.440670062538711 2316 88-89 -0.34348872458331314 2316 90-91 -0.33168464433778055 2316 92-93 0.063451640316881 2316 94-95 0.0014256530512604115 2316 96-97 0.3353571050374171 2316 98-99 0.4920172850481208 2316 100-101 -0.4825505179208065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 5.0 4 11.0 5 31.0 6 51.0 7 138.0 8 384.0 9 713.0 10 1186.0 11 1924.0 12 2863.0 13 4033.0 14 5321.0 15 7366.0 16 9770.0 17 13570.0 18 12571.0 19 8386.0 20 7602.0 21 7809.0 22 8062.0 23 9105.0 24 10448.0 25 12833.0 26 15661.0 27 19345.0 28 24191.0 29 30474.0 30 38316.0 31 48113.0 32 61600.0 33 81000.0 34 111378.0 35 158190.0 36 243105.0 37 365554.0 38 295248.0 39 27293.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.54086328736448 19.833192185761973 15.464395804172815 22.16154872270073 2 15.65115550404434 20.81556044303701 39.64627613459029 23.887007918328358 3 19.53998862291661 24.38394918641686 30.117269134946202 25.95879305572033 4 13.076649296841492 17.23451697588606 37.22545181318464 32.463381914087805 5 13.506062873796095 37.77824423068733 34.40451201927465 14.31118087624193 6 31.78659755240607 36.98939254283898 17.092090493442967 14.131919411311985 7 28.481463567476144 32.80104401471112 20.567333169065286 18.150159248747457 8 26.36051969543487 35.550221913966126 19.219179207315403 18.870079183283597 9 25.89186903577697 17.09622761467583 20.243481752557564 36.76842159698964 10-11 24.997642446428777 26.991554786807193 27.23908270176248 20.771720065001553 12-13 26.018625672177947 24.001240007836753 27.974102211205338 22.006032108779962 14-15 22.541591757394343 25.796502307357688 26.364170096522688 25.297735838725284 16-17 21.862586735050847 29.087856028181097 27.01610131079819 22.033455925969868 18-19 21.88038244035397 27.70012563463744 29.207619603870643 21.21187232113795 20-21 22.619406140583028 26.602419607521043 29.364282650556227 21.413891601339696 22-23 22.357251196886907 26.362936143520564 29.376341155205004 21.90347150438752 24-25 22.363125524369373 26.57949637420824 29.43471232464997 21.622665776772422 26-27 22.15257009651418 26.692268309844376 29.570384593505466 21.58477700013598 28-29 21.79409605703103 27.2968152463211 29.443449167724562 21.465639528923308 30-31 22.18606616447028 26.65576205102496 29.29267333334753 21.86549845115723 32-33 22.066035755678655 26.75926517426102 29.249629636389628 21.92506943367069 34-35 22.520966991248166 26.716312121461012 29.154202067952216 21.608518819338606 36-37 22.465606324937905 26.549590013704233 29.29831579363643 21.686487867721432 38-39 22.06075834617321 26.931677661048553 29.384127022755862 21.623436970022375 40-41 22.217270510202436 26.96937276545545 29.103675566262073 21.709681158080045 42-43 22.165369375158207 26.793146503884575 28.918556623245127 22.122927497712094 44-45 22.36758610241943 26.75224028429358 28.80501541383735 22.07515819944964 46-47 22.608576617355833 27.312300939065683 28.416608108149216 21.66251433542927 48-49 22.551576380559297 27.112175946952355 28.42178815560083 21.914459516887522 50-51 22.31943443119146 27.38375754035975 28.511457696414393 21.785350332034398 52-53 22.718332010062937 27.668580085236865 27.751900490066344 21.86118741463385 54-55 22.514274589253827 27.380107139271924 27.90771177649811 22.197906494976134 56-57 22.547614919189247 27.55772957220341 27.93877060575364 21.9558849028537 58-59 22.96062738226696 27.197708764917213 27.939652786016527 21.902011066799297 60-61 22.55476362131956 27.335268045909878 28.434190873388882 21.67577745938168 62-63 22.22604500336141 27.543371327924653 28.37210363488688 21.85848003382705 64-65 22.200627382648662 27.66087023181278 28.1028732324443 22.035629153094263 66-67 22.39791805457958 27.508662097581304 27.80915094712698 22.28426890071213 68-69 22.23730040671552 27.41940979098412 28.003960685180285 22.339329117120077 70-71 22.546337278808508 27.473131526993193 27.832209313998373 22.14832188019992 72-73 22.599754814726936 27.28845165195859 27.953615570177444 22.15817796313703 74-75 22.201222275964238 28.008128226422212 27.58793664120512 22.202712856408432 76-77 22.355999282087787 27.57208781648217 27.63289741460343 22.439015486826616 78-79 22.517438270196603 27.5937164429275 27.72239308127314 22.166452205602756 80-81 22.54810849847094 27.67525048603584 27.663873399184563 22.11276761630866 82-83 22.464315678104025 27.626853421868052 27.77658125305342 22.132249646974504 84-85 22.44707429842816 27.619287213780808 27.70589332208194 22.22774516570909 86-87 22.30951750823622 27.657203001846494 27.66559892434848 22.367680565568808 88-89 22.308331127882678 27.60320748575583 27.49177899255014 22.596682393811353 90-91 22.441888694253272 27.402805064960905 27.65403306425754 22.501273176528283 92-93 22.612457521083932 27.331644164870063 27.82008135144023 22.235816962605778 94-95 22.367315525460025 27.337427866553504 27.756646011480512 22.538610596505958 96-97 22.04008748794607 27.532328864634003 27.988537740584245 22.43904590683568 98-99 22.20997308739342 27.597513709550096 27.46457814861831 22.727935054438177 100-101 22.054746843915275 27.441691228189736 27.762045692951347 22.741516234943646 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 432.0 1 301.5 2 222.0 3 287.5 4 244.5 5 225.0 6 440.0 7 1032.5 8 1729.0 9 2049.5 10 1860.5 11 1660.0 12 1718.0 13 1866.0 14 2062.5 15 2205.0 16 2273.0 17 2335.5 18 2505.5 19 2660.5 20 2936.5 21 3389.5 22 4032.0 23 4917.5 24 6042.5 25 7497.5 26 9461.5 27 12169.0 28 14431.5 29 16923.0 30 20286.0 31 22559.0 32 24950.5 33 28045.0 34 31365.5 35 34511.5 36 38157.5 37 41707.5 38 43929.5 39 45667.5 40 47517.5 41 50797.5 42 53864.0 43 56745.0 44 59895.0 45 62404.0 46 63549.0 47 67307.5 48 75066.5 49 73773.0 50 65525.5 51 60397.5 52 57327.0 53 55282.5 54 53364.0 55 49320.0 56 43082.0 57 37880.5 58 33779.5 59 29436.5 60 24570.0 61 20141.0 62 15967.0 63 11983.5 64 8954.0 65 6676.0 66 4788.0 67 3715.5 68 2911.0 69 1977.0 70 1383.0 71 940.5 72 677.5 73 516.5 74 414.5 75 342.0 76 224.0 77 109.0 78 58.0 79 43.5 80 28.0 81 17.5 82 12.0 83 7.5 84 3.5 85 1.5 86 1.0 87 0.5 88 1.0 89 1.0 90 0.5 91 0.5 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07093946113995941 2 0.0 3 0.0 4 0.0 5 0.002737800815864643 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.216800362606508E-4 12-13 0.0071182821212480716 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 9.734402900852064E-4 24-25 8.517602538245557E-4 26-27 6.388201903684167E-4 28-29 0.0035591410606240358 30-31 5.17140154107766E-4 32-33 0.0 34-35 0.0 36-37 1.521000453258135E-4 38-39 7.605002266290675E-4 40-41 2.1294006345613892E-4 42-43 0.014054044188105169 44-45 1.216800362606508E-4 46-47 0.0 48-49 3.04200090651627E-5 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.08400181303254E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 3.04200090651627E-5 82-83 3.6504010878195243E-4 84-85 3.9546011784711513E-4 86-87 0.0 88-89 0.0 90-91 0.007787522320681651 92-93 0.002768220824929806 94-95 0.0 96-97 0.0 98-99 9.126002719548811E-5 100-101 0.00991692295524304 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1643655.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 56.164593882665045 #Duplication Level Percentage of deduplicated Percentage of total 1 76.49960896534648 42.96569469721368 2 13.224971982165885 14.855503609759415 3 4.2312078972395675 7.129322195447562 4 1.9535239010672822 4.388755061740937 5 1.0673418832819535 2.99734117042449 6 0.6857856425763041 2.3110123263516367 7 0.45259966241101274 1.779405336152208 8 0.30899717566359725 1.388376070562916 9 0.22616092920788264 1.14320130669782 >10 1.2398045873270873 12.477487234100302 >50 0.07495095442431426 2.878020121056202 >100 0.03245063277500869 3.0966274651401924 >500 0.001297893256835526 0.5591703640266339 >1k 9.734199426266445E-4 0.9719419689762241 >5k 3.244733142088815E-4 1.058141072349803 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6607 0.40196999978705994 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5628 0.3424076220374714 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 5184 0.31539465398760685 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4040 0.24579367324651463 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 2056 0.12508707727594903 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 1817 0.11054631294280125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 6.08400181303254E-5 2 0.0 0.0 0.0 0.0 6.08400181303254E-5 3 0.0 0.0 0.0 0.0 6.08400181303254E-5 4 0.0 0.0 0.0 0.0 6.08400181303254E-5 5 0.0 0.0 0.0 0.0 6.08400181303254E-5 6 0.0 0.0 0.0 0.0 6.08400181303254E-5 7 0.0 0.0 0.0 0.0 6.08400181303254E-5 8 0.0 0.0 0.0 0.0 6.08400181303254E-5 9 0.0 0.0 0.0 6.08400181303254E-5 6.08400181303254E-5 10-11 0.0 0.0 0.0 6.08400181303254E-5 6.08400181303254E-5 12-13 0.0 0.0 0.0 1.216800362606508E-4 6.08400181303254E-5 14-15 0.0 0.0 0.0 1.216800362606508E-4 6.08400181303254E-5 16-17 0.0 0.0 0.0 1.521000453258135E-4 6.08400181303254E-5 18-19 0.0 0.0 0.0 2.433600725213016E-4 6.08400181303254E-5 20-21 0.0 0.0 0.0 2.433600725213016E-4 6.08400181303254E-5 22-23 0.0 0.0 0.0 3.6504010878195243E-4 6.08400181303254E-5 24-25 0.0 0.0 0.0 5.17140154107766E-4 6.08400181303254E-5 26-27 0.0 0.0 0.0 7.909202356942303E-4 6.08400181303254E-5 28-29 0.0 0.0 0.0 0.0015514204623232977 6.08400181303254E-5 30-31 0.0 0.0 0.0 0.003133260933711758 6.08400181303254E-5 32-33 0.0 0.0 0.0 0.005779801722380913 6.08400181303254E-5 34-35 0.0 0.0 0.0 0.007817942329746815 6.08400181303254E-5 36-37 0.0 0.0 0.0 0.009521462837395925 6.08400181303254E-5 38-39 0.0 0.0 0.0 0.011103303308784385 6.08400181303254E-5 40-41 0.0 0.0 0.0 0.012441783707651545 6.08400181303254E-5 42-43 0.0 0.0 0.0 0.014358244278756795 6.08400181303254E-5 44-45 0.0 0.0 0.0 0.01651806492238335 6.08400181303254E-5 46-47 0.0 0.0 0.0 0.019560065828899617 6.08400181303254E-5 48-49 0.0 0.0 0.0 0.02333214695297979 6.08400181303254E-5 50-51 0.0 0.0 0.0 0.031150089282726605 6.08400181303254E-5 52-53 0.0 0.0 0.0 0.048276554386413206 6.08400181303254E-5 54-55 0.0 0.0 0.0 0.07036148096772132 6.08400181303254E-5 56-57 0.0 0.0 0.0 0.09700940890880386 9.12600271954881E-5 58-59 0.0 0.0 0.0 0.13059309891674348 1.216800362606508E-4 60-61 0.0 0.0 0.0 0.18099905393771806 1.216800362606508E-4 62-63 0.0 0.0 0.0 0.21440022389126673 1.216800362606508E-4 64-65 0.0 0.0 0.0 0.2499612144884419 1.216800362606508E-4 66-67 0.0 0.0 0.0 0.31971429527485995 1.216800362606508E-4 68-69 0.0 0.0 0.0 0.40933164198082933 1.216800362606508E-4 70-71 0.0 0.0 0.0 0.4867201450426032 1.216800362606508E-4 72-73 0.0 0.0 0.0 0.5645345282312895 2.433600725213016E-4 74-75 0.0 0.0 0.0 0.7030672495140404 2.433600725213016E-4 76-77 0.0 0.0 0.0 0.8061910802449419 2.433600725213016E-4 78-79 0.0 0.0 0.0 0.9009494084829237 2.433600725213016E-4 80-81 0.0 0.0 0.0 1.023998345151507 2.433600725213016E-4 82-83 0.0 0.0 0.0 1.2169524626518338 2.433600725213016E-4 84-85 0.0 0.0 0.0 1.4097544801068351 2.433600725213016E-4 86-87 0.0 0.0 0.0 1.656521593643435 2.433600725213016E-4 88-89 0.0 0.0 0.0 1.9815289704956331 2.433600725213016E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 6045 0.0 24.299105 1 TACATGG 6070 0.0 23.632122 2 ACATGGG 6030 0.0 22.607317 3 GAGTACT 6275 0.0 21.615042 12-13 GTATACG 110 0.0028650537 21.607544 1 AGTACTT 6495 0.0 21.065756 12-13 GTACTTT 6615 0.0 20.499075 14-15 CCATATA 675 0.0 20.406975 2 GTATCAA 12890 0.0 20.357021 1 AGAGTAC 9785 0.0 20.318813 10-11 ACCGTGC 355 7.8216544E-11 18.735418 8 GTATAGG 500 0.0 18.063906 1 CAACGCA 14625 0.0 17.86287 5 TCAACGC 14680 0.0 17.795946 4 ATCAACG 14745 0.0 17.74971 3 CACCGTT 270 4.868325E-7 17.595434 7 TATCAAC 14870 0.0 17.56856 2 GGTATCA 10395 0.0 17.514687 1 AACGCAG 14950 0.0 17.473484 6 TACTTTT 7850 0.0 17.364817 14-15 >>END_MODULE