FastQCFastQC Report
Thu 26 May 2016
SRR937371_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937371_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515542
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31870.618184357433536No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24360.47251242381804004No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17380.3371209329210811No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9700.18815149881096013No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9240.17922885041373932No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7350.14256840373820173No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT7100.13771913830492957No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.10241648595070818No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC5210.10105869162939198No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC250.004993732657.0747341
ACACGGC250.005013012357.0192033
GTATCAA34000.033.1537061
GGTATCA23000.029.9849151
GGGACCG650.005831497729.2406185
GTACATG21850.027.2095431
ATCAACG42200.026.3477383
TCAACGC42300.026.2880064
TATCAAC42350.026.2544162
CAACGCA42850.025.9480635
AACGCAG43600.025.6032186
TACATGG22800.025.425232
ACATGGG22550.025.285683
TGCACCG1351.3373541E-524.637935
GGGCGCG1201.7051242E-423.7580017
CCGACGC701.7799633E-523.74876294-95
GAGTACT25500.023.28309812-13
AGTACTT25400.023.18776512-13
ACGCAGA47950.022.9900177
CCGTGTG1252.2484126E-422.807689
Produced by FastQC (version 0.11.5)