##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937371_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 515542 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.830347478963887 33.0 31.0 34.0 30.0 34.0 2 31.952609098773717 33.0 31.0 34.0 30.0 34.0 3 31.97339305042072 34.0 31.0 34.0 30.0 34.0 4 35.60320788606942 37.0 35.0 37.0 33.0 37.0 5 35.60412148767705 37.0 35.0 37.0 33.0 37.0 6 35.64622087046254 37.0 35.0 37.0 33.0 37.0 7 35.58913725748823 37.0 35.0 37.0 33.0 37.0 8 35.5706014252961 37.0 35.0 37.0 33.0 37.0 9 37.20685802514635 39.0 37.0 39.0 34.0 39.0 10-11 37.09912868398695 39.0 37.0 39.0 33.5 39.0 12-13 36.9694050145284 39.0 37.0 39.0 33.0 39.0 14-15 38.090983857765224 40.0 38.0 41.0 33.0 41.0 16-17 38.031747170938544 40.0 38.0 41.0 33.0 41.0 18-19 37.99102788909536 40.0 38.0 41.0 33.0 41.0 20-21 37.89449841138064 40.0 38.0 41.0 33.0 41.0 22-23 37.95220563988967 40.0 38.0 41.0 33.0 41.0 24-25 37.91059991232528 40.0 38.0 41.0 33.0 41.0 26-27 37.8211319349345 40.0 38.0 41.0 33.0 41.0 28-29 37.670623343975855 40.0 37.0 41.0 33.0 41.0 30-31 37.4000391820647 39.5 37.0 41.0 32.0 41.0 32-33 37.131594516062705 39.0 36.0 41.0 31.5 41.0 34-35 37.127864461091434 39.5 36.0 41.0 32.0 41.0 36-37 36.99100170306202 39.5 36.0 41.0 31.0 41.0 38-39 36.83828863603741 39.0 36.0 41.0 31.0 41.0 40-41 36.60271714040758 39.0 35.5 41.0 30.5 41.0 42-43 36.32643121219998 39.0 35.0 41.0 30.0 41.0 44-45 36.010138844167884 39.0 35.0 41.0 29.5 41.0 46-47 35.53110512819518 39.0 35.0 40.0 27.5 41.0 48-49 35.12308890449275 39.0 35.0 40.0 25.5 41.0 50-51 34.12897979214108 38.0 34.0 39.5 23.5 40.5 52-53 34.20983062485695 38.0 34.0 39.5 24.0 40.5 54-55 34.512609059979596 38.0 34.0 40.0 23.5 41.0 56-57 34.25446326390478 38.0 34.0 40.0 22.5 41.0 58-59 33.98112471922753 38.0 34.0 40.0 22.0 41.0 60-61 33.64296022438521 38.0 33.0 40.0 20.5 41.0 62-63 33.270139969197466 37.0 33.0 40.0 18.5 41.0 64-65 32.84075109302442 36.5 32.0 39.5 16.0 41.0 66-67 32.403990363539734 36.0 32.0 39.0 12.0 40.5 68-69 31.89816348619511 35.5 31.0 39.0 8.0 40.0 70-71 31.41954001807806 35.0 31.0 38.0 6.5 40.0 72-73 30.926149566863614 35.0 30.5 37.5 2.0 40.0 74-75 30.38308033099146 35.0 30.0 37.0 2.0 39.0 76-77 29.864540619386975 34.0 29.0 36.5 2.0 39.0 78-79 29.338433144147324 34.0 29.0 36.0 2.0 38.5 80-81 28.764281086700986 34.0 28.5 35.5 2.0 37.0 82-83 28.22254733852916 34.0 27.0 35.0 2.0 37.0 84-85 27.67683137358353 33.0 26.0 35.0 2.0 36.0 86-87 27.108597359671958 33.0 25.5 35.0 2.0 36.0 88-89 26.573485768375804 32.5 25.0 35.0 2.0 35.5 90-91 25.996329106067012 32.0 23.5 35.0 2.0 35.0 92-93 25.347574009489044 32.0 20.5 34.0 2.0 35.0 94-95 24.584545972976013 31.5 16.5 34.0 2.0 35.0 96-97 23.722578373827933 31.0 4.0 34.0 2.0 35.0 98-99 22.51836222848963 31.0 2.0 34.0 2.0 35.0 100-101 19.441880583929148 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.010335700851886287 1101 2 0.08955100692855922 1101 3 -0.09140623788792723 1101 4 -0.11538299765044968 1101 5 -0.03880154114499135 1101 6 -0.06808470825635737 1101 7 -0.073889899030533 1101 8 -0.012613928912671213 1101 9 -0.056182585479170655 1101 10-11 0.11109458473725908 1101 12-13 0.07210129685417854 1101 14-15 -0.04633540567687788 1101 16-17 -0.027704969392686962 1101 18-19 -0.023733744563038783 1101 20-21 0.010711154247019294 1101 22-23 -0.13212427066132193 1101 24-25 0.010134021638336321 1101 26-27 -0.06576011747908694 1101 28-29 -0.06328591438280995 1101 30-31 0.14237065343979793 1101 32-33 -0.05072741510431911 1101 34-35 0.004331696806410434 1101 36-37 -0.04715586489034962 1101 38-39 0.18997868477050162 1101 40-41 0.2972532771127874 1101 42-43 0.48682539431978 1101 44-45 0.23554651201810373 1101 46-47 0.6632647598734351 1101 48-49 0.6593827696605032 1101 50-51 0.6597292409999014 1101 52-53 0.6980954566264543 1101 54-55 0.8098509367522553 1101 56-57 0.8474774721712919 1101 58-59 0.7952596918715145 1101 60-61 0.9674465755229065 1101 62-63 0.9950088097252561 1101 64-65 0.7742831482765524 1101 66-67 0.9532565719546966 1101 68-69 0.9543317529521147 1101 70-71 0.7985401692660616 1101 72-73 0.7959476478419525 1101 74-75 0.7821557471904903 1101 76-77 0.8148834287199129 1101 78-79 0.7906566340813974 1101 80-81 1.0011635968028472 1101 82-83 0.8744155074004532 1101 84-85 1.2480072967082947 1101 86-87 1.4350553718509467 1101 88-89 1.297716382303907 1101 90-91 1.2837467489070384 1101 92-93 1.3526333033225626 1101 94-95 1.5750559470922596 1101 96-97 1.7088442558078292 1101 98-99 1.9810554780723173 1101 100-101 1.8335258677708879 1102 1 0.007274592302838556 1102 2 -0.033278121915852665 1102 3 -0.06632538967632584 1102 4 -0.09337089643717889 1102 5 -0.1628050815662334 1102 6 -0.09792335556337406 1102 7 -0.08700511644466502 1102 8 -0.06527148863818155 1102 9 -0.025134198767396754 1102 10-11 -0.0812176979846626 1102 12-13 -0.08994172098255859 1102 14-15 -0.055480061674536785 1102 16-17 0.0892449405213469 1102 18-19 0.07772539021072333 1102 20-21 0.08754536075058184 1102 22-23 0.023007382786055075 1102 24-25 0.023515152568037934 1102 26-27 0.04076330916745263 1102 28-29 -0.07099167749731095 1102 30-31 0.09772248518768123 1102 32-33 0.033838811683153835 1102 34-35 0.1763529164185158 1102 36-37 0.17255839981820742 1102 38-39 0.03698436491234958 1102 40-41 0.09668526292819024 1102 42-43 0.26115934541270036 1102 44-45 0.2938297896384938 1102 46-47 0.4833830985489058 1102 48-49 0.20755608225544364 1102 50-51 -0.05086471526806946 1102 52-53 0.10150869768731496 1102 54-55 0.3539324275484006 1102 56-57 0.4066515839389879 1102 58-59 0.599041297148915 1102 60-61 0.5233795977803908 1102 62-63 0.3885937535778723 1102 64-65 0.15752754141607994 1102 66-67 0.5785863381322187 1102 68-69 0.6476790455515129 1102 70-71 0.6340199068471328 1102 72-73 0.5576619259177882 1102 74-75 0.32873166907970486 1102 76-77 0.4112419889868022 1102 78-79 0.41330769157696423 1102 80-81 0.40584911707195204 1102 82-83 0.8011441600830302 1102 84-85 0.840678395585158 1102 86-87 0.7155330231805621 1102 88-89 1.0635835897727794 1102 90-91 0.9979049965703872 1102 92-93 1.2015084939257434 1102 94-95 0.8634498962946608 1102 96-97 0.7453183285239575 1102 98-99 0.7892855252492907 1102 100-101 0.8590993959610635 1103 1 -0.029089813087850303 1103 2 0.005379546173035266 1103 3 -0.005146163596311482 1103 4 0.002735976213386948 1103 5 -0.12785760851702577 1103 6 -0.023432631711855834 1103 7 -0.14123846616789137 1103 8 -0.12647186296209867 1103 9 -0.11453972163128867 1103 10-11 -0.02508100631020227 1103 12-13 -0.05415757053395254 1103 14-15 -0.08853344512920813 1103 16-17 -0.14203225479854353 1103 18-19 -0.05274803966434405 1103 20-21 -0.19960351231237183 1103 22-23 -0.056665982586871166 1103 24-25 -0.13599349442018394 1103 26-27 -0.08519629239963677 1103 28-29 -0.4934179362143567 1103 30-31 -0.5014654222464614 1103 32-33 -0.359299111876517 1103 34-35 -0.06927600591984628 1103 36-37 -0.13257110674039296 1103 38-39 -0.14261709643131582 1103 40-41 -0.044536761910421774 1103 42-43 -0.20403130600607966 1103 44-45 0.048682650731542765 1103 46-47 -0.12754869307744343 1103 48-49 -0.15113003022148774 1103 50-51 -0.2341168922154253 1103 52-53 -0.285879547061306 1103 54-55 0.02513737518005854 1103 56-57 0.20481142236641148 1103 58-59 0.05509260278861916 1103 60-61 -0.006165820716923065 1103 62-63 -0.00693061712969012 1103 64-65 0.047561299300411974 1103 66-67 0.4399655472936317 1103 68-69 0.4588324972092721 1103 70-71 0.408167236088282 1103 72-73 0.576567795084177 1103 74-75 0.5921489080707225 1103 76-77 0.4908248134405149 1103 78-79 0.5191553131925808 1103 80-81 0.22573481081365543 1103 82-83 0.4135979643877583 1103 84-85 0.3759893540764381 1103 86-87 0.4366947490887476 1103 88-89 0.770197306523773 1103 90-91 0.48866387486753027 1103 92-93 0.775602688204085 1103 94-95 0.6226632609273963 1103 96-97 0.8260563758008566 1103 98-99 0.7818208560342619 1103 100-101 0.9488069421831931 1104 1 0.1178978007716367 1104 2 0.09390206709956317 1104 3 0.08950668492074598 1104 4 0.08421655551273233 1104 5 0.1588837588302141 1104 6 0.13471948603767459 1104 7 0.11773004439502444 1104 8 0.24165781505730166 1104 9 0.23710339808171454 1104 10-11 0.18421745686354285 1104 12-13 0.10466626282153157 1104 14-15 0.36064868575937226 1104 16-17 0.19040791822359182 1104 18-19 0.1900900694931309 1104 20-21 0.10978242151995232 1104 22-23 0.2775199128617061 1104 24-25 0.1533110953904071 1104 26-27 -0.041001465347946464 1104 28-29 0.1351325605599314 1104 30-31 0.03629089999987656 1104 32-33 0.14866551602256095 1104 34-35 0.2597945168372817 1104 36-37 0.40576097173960335 1104 38-39 0.29540791205740646 1104 40-41 0.43533945049934175 1104 42-43 0.45063121226310443 1104 44-45 0.27707887436116607 1104 46-47 0.6718482391752403 1104 48-49 0.8163581963016853 1104 50-51 0.8337669821811389 1104 52-53 0.8038646104892209 1104 54-55 0.725764432770454 1104 56-57 1.0154245289403647 1104 58-59 1.0548896118088038 1104 60-61 1.257785195714277 1104 62-63 1.007413463653343 1104 64-65 1.1788650919100547 1104 66-67 1.3299352093690189 1104 68-69 0.8963869356993115 1104 70-71 0.9678982545862951 1104 72-73 1.106449912686081 1104 74-75 1.4077592834933164 1104 76-77 1.44954567500805 1104 78-79 1.49657348937399 1104 80-81 1.4110877198589336 1104 82-83 1.4771359864436455 1104 84-85 1.5525915065189722 1104 86-87 1.3310471709556744 1104 88-89 1.5039045123344827 1104 90-91 1.5361582280275456 1104 92-93 1.534888602844532 1104 94-95 1.4389693417990053 1104 96-97 1.6742357312491336 1104 98-99 1.6388174014968584 1104 100-101 1.2788056873298679 1105 1 -0.06490549905591436 1105 2 -0.0076768993480520464 1105 3 -0.07053909423109772 1105 4 -0.1384234101011188 1105 5 0.024886524222416995 1105 6 -0.1638496565353833 1105 7 0.045348233839924035 1105 8 -0.11320052332520447 1105 9 -0.3493668946666375 1105 10-11 -0.2633074135410638 1105 12-13 -0.30957550702948566 1105 14-15 -0.23969517574315802 1105 16-17 -0.33746868521191686 1105 18-19 -0.18415579148118155 1105 20-21 -0.13362776278773936 1105 22-23 0.09593488740870271 1105 24-25 0.12153756754746325 1105 26-27 -0.02220301272470948 1105 28-29 -0.15366708314983413 1105 30-31 -0.03612198237774322 1105 32-33 -0.33270433705950353 1105 34-35 -0.12896447457135451 1105 36-37 -0.02745532439558218 1105 38-39 -0.15695639470434486 1105 40-41 -0.11656861833510845 1105 42-43 -0.005198897368636324 1105 44-45 -0.07879188757171107 1105 46-47 0.09417645083649262 1105 48-49 -0.053665780788030304 1105 50-51 -0.4582058714032584 1105 52-53 -0.32978306611525454 1105 54-55 -0.17193029693059003 1105 56-57 -0.2670895440059198 1105 58-59 -0.12505378568783954 1105 60-61 0.07014763816667369 1105 62-63 0.11303565100606505 1105 64-65 0.21206274495173716 1105 66-67 0.4315944446914628 1105 68-69 0.4390459586976405 1105 70-71 0.25120336434751067 1105 72-73 0.42900391292429774 1105 74-75 0.17171182646305638 1105 76-77 0.2402419945506722 1105 78-79 0.1599077030756284 1105 80-81 -0.047753767238489786 1105 82-83 0.20472413979345205 1105 84-85 0.20064226751969372 1105 86-87 0.1837253031248487 1105 88-89 0.5264976112827036 1105 90-91 0.539564476571055 1105 92-93 0.619947716986875 1105 94-95 0.8679909418942877 1105 96-97 0.6881041213662229 1105 98-99 0.9945302149986013 1105 100-101 0.4407028292400561 1106 1 -0.04591314563504767 1106 2 -0.5404831269174935 1106 3 -0.13533607184288954 1106 4 0.06827453623494506 1106 5 -0.049333749258018145 1106 6 -0.3391536878308159 1106 7 -0.2304959035857621 1106 8 -0.15869786612910985 1106 9 -0.14963793260800884 1106 10-11 -0.5949152437011236 1106 12-13 -0.3167020715053894 1106 14-15 -0.3004226447294016 1106 16-17 -0.29083676497901223 1106 18-19 -0.007880672212671413 1106 20-21 0.00881685762125528 1106 22-23 0.05773941705150776 1106 24-25 -0.22277319636194193 1106 26-27 -0.47372346466471527 1106 28-29 0.12468651893652805 1106 30-31 0.26961708845062304 1106 32-33 0.24816582079645855 1106 34-35 0.09571731191535804 1106 36-37 0.27390104716277364 1106 38-39 0.4228737338417048 1106 40-41 0.40842557089507636 1106 42-43 0.2060669049706334 1106 44-45 0.46041209605571254 1106 46-47 0.9296568143740132 1106 48-49 1.1184728582705858 1106 50-51 0.8705123318673245 1106 52-53 0.8943255223995195 1106 54-55 1.2267239681744613 1106 56-57 0.7396962532907949 1106 58-59 0.5565357257741539 1106 60-61 0.877876159000742 1106 62-63 0.8980548954878103 1106 64-65 1.0955479462013429 1106 66-67 1.3113942390493065 1106 68-69 1.3709634339476935 1106 70-71 1.1978250418675103 1106 72-73 1.1975304198834316 1106 74-75 1.018358716814813 1106 76-77 1.2020977377579811 1106 78-79 1.2424757875701324 1106 80-81 1.6652808728286388 1106 82-83 1.579828044236816 1106 84-85 1.0228425598309627 1106 86-87 1.233177908910406 1106 88-89 1.4177790928517275 1106 90-91 1.1606215245424032 1106 92-93 1.3615358890171727 1106 94-95 1.2835279555131578 1106 96-97 1.2529779193247705 1106 98-99 1.471432897407528 1106 100-101 0.8207606611728941 1107 1 -0.03951822025111795 1107 2 0.039559333734086266 1107 3 -0.08035934255278931 1107 4 0.04142411446947136 1107 5 -0.2585428550532285 1107 6 -0.10811505483651018 1107 7 -0.06025038597389454 1107 8 -0.33117489243714715 1107 9 -0.39099212779270687 1107 10-11 -0.18134488144254846 1107 12-13 -0.1825971802656099 1107 14-15 -0.4300438564592852 1107 16-17 -0.56998972146976 1107 18-19 -0.3170685993373823 1107 20-21 -0.10025329543337591 1107 22-23 -0.03476746601640457 1107 24-25 -0.14813387945154943 1107 26-27 -0.203442304900058 1107 28-29 -0.022066171012902203 1107 30-31 -0.23163038689688875 1107 32-33 -0.3408672437571596 1107 34-35 -0.19364273064860527 1107 36-37 -0.07578979590328316 1107 38-39 -0.023236880804638815 1107 40-41 0.018123335770511062 1107 42-43 -0.0582997236432945 1107 44-45 -0.05575084540333819 1107 46-47 -0.021366218721531993 1107 48-49 -0.4507788114867921 1107 50-51 -0.6232012188659226 1107 52-53 -0.3394275962888571 1107 54-55 -0.33563171984823015 1107 56-57 0.018138949490513312 1107 58-59 0.06789472490117987 1107 60-61 0.17009532208167144 1107 62-63 -0.11326536216879646 1107 64-65 -0.2416035742722329 1107 66-67 0.31218198228116734 1107 68-69 0.3142820799591348 1107 70-71 0.23066231219516098 1107 72-73 0.38549370330053634 1107 74-75 0.3329472049160138 1107 76-77 0.25215688306948536 1107 78-79 0.2894898533066588 1107 80-81 -0.007498336948678741 1107 82-83 0.1636620034601286 1107 84-85 0.12658705208525234 1107 86-87 -0.0028893669443590397 1107 88-89 0.17117155427813913 1107 90-91 -0.020980409431796687 1107 92-93 -0.24572374598652047 1107 94-95 -0.08419575065086704 1107 96-97 0.4467472577754208 1107 98-99 0.7233531829579967 1107 100-101 0.9173922194581472 1108 1 0.06873641962996047 1108 2 0.12728685598516165 1108 3 0.24821640362150887 1108 4 -0.041708244730415345 1108 5 0.09866476613263586 1108 6 0.07746785239054077 1108 7 0.21092591408716999 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0.8240954937100149 2316 88-89 0.45739459821103523 2316 90-91 0.3172074154427982 2316 92-93 0.48818217487668036 2316 94-95 -0.04209654929302431 2316 96-97 0.5192501750166976 2316 98-99 1.3748943956210944 2316 100-101 1.8881705545847645 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2161.0 3 306.0 4 305.0 5 332.0 6 380.0 7 454.0 8 563.0 9 763.0 10 984.0 11 1373.0 12 1872.0 13 2533.0 14 2905.0 15 3965.0 16 5174.0 17 7097.0 18 6000.0 19 3921.0 20 3922.0 21 4085.0 22 4364.0 23 4863.0 24 5411.0 25 6160.0 26 7129.0 27 8360.0 28 9682.0 29 11673.0 30 14360.0 31 17513.0 32 22163.0 33 28816.0 34 38563.0 35 52813.0 36 72796.0 37 92341.0 38 63108.0 39 6332.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.685497921878195 19.236691746887686 15.517681069137701 22.56012926209642 2 14.698385891728668 20.39465492015067 40.959943031472775 23.947016156647884 3 18.05525020963484 25.411049652813585 31.41059439015232 25.123105747399265 4 11.930651942558859 16.743419580650873 38.57587837824692 32.75005009854335 5 12.660269505698688 37.47786870255147 35.18263427617524 14.679227515574597 6 30.09413229087656 36.7977517163584 18.19281560573351 14.915300387031527 7 26.615971060718035 33.69928818701622 21.863549729666655 17.821191022599088 8 25.69962133220271 33.7749503569824 20.851039829161895 19.67438848165299 9 25.893442415683893 16.28979959424789 21.315018079893875 36.501739910174344 10-11 23.951346851122764 26.585331694184656 28.264518915049564 21.19880253964302 12-13 25.46130700572026 24.330771759278168 28.63487747429187 21.573043760709695 14-15 22.2045247249595 25.343107797206127 27.871905125856433 24.58046235197794 16-17 20.869116239994398 28.835188599254778 28.179330704067585 22.116364456683236 18-19 20.529460719688572 27.05725308101844 31.412387852484454 21.000898346808537 20-21 21.534654668669575 25.974108050033788 31.335287626214747 21.15594965508189 22-23 21.08696307401822 25.937053057946542 31.39714754350195 21.578836324533285 24-25 21.205088466751317 26.036001174500882 31.31969953000519 21.439210828742603 26-27 20.818546858137342 26.271085918738397 31.472878768728187 21.43748845439608 28-29 20.501936621943972 26.59108832999299 31.505916834656343 21.401058213406696 30-31 20.661362796397515 26.288004045984163 31.325837888307497 21.72479526931082 32-33 20.65054708234097 26.45795425922882 31.061498259703406 21.8300003987268 34-35 21.289897832763145 26.38960473162867 30.792637268383004 21.527860167225185 36-37 21.15301594215863 26.1423787614199 30.994838925469725 21.709766370951748 38-39 20.82127830016255 26.48364404209151 30.99076817782341 21.704309479922536 40-41 20.93495085320317 26.491926820803517 30.966321976409926 21.606800349583388 42-43 20.926431524457357 26.6605994842895 30.335522963896377 22.07744602735677 44-45 20.816043184213495 26.790040874106975 30.342315784561176 22.051600157118358 46-47 21.09467530421693 27.015358165966212 29.754331578466285 22.135634951350575 48-49 21.168212574856124 27.150770296001387 29.430150169307577 22.250866959834916 50-51 21.17643282862078 27.332091003874826 29.37532095672336 22.116155210781034 52-53 21.528529694171485 27.4152203119087 28.956280054361734 22.099969939558083 54-55 20.933670204592886 27.1583975664358 29.355451693400475 22.552480535570844 56-57 21.20210801998361 27.338347706638743 29.054200534101724 22.40534373927592 58-59 21.496043857773284 27.069052664320846 29.05295592838119 22.381947549524682 60-61 21.42464853284394 26.794419295481255 29.764794281265093 22.016137890409706 62-63 21.16045268697835 26.99508701256535 29.7729565996761 22.071503700780205 64-65 20.97672212350844 27.11899827758489 29.629914034767125 22.27436556413955 66-67 20.851551943696474 27.034830029843203 29.308648695939578 22.80496933052075 68-69 20.815268742782266 26.96857052869791 29.663191784710257 22.552968943809564 70-71 20.982486565515952 27.113257949870516 29.23993094169706 22.664324542916468 72-73 20.97369393191142 26.819551649719052 29.375984290241675 22.830770128127856 74-75 20.696836063278734 27.23470530589388 29.10007077636225 22.968387854465135 76-77 20.910903026943984 26.809019573524694 29.248855050902833 23.031222348628493 78-79 20.83140891071684 26.755194968724687 29.30087792564906 23.112518194909413 80-81 20.841378452109424 27.009724142356312 29.09814947684853 23.050747928685734 82-83 20.85666456685565 26.827224438689406 29.279434295357866 23.03667669909708 84-85 20.897447486414165 26.59868646781924 28.915106397661805 23.588759648104787 86-87 20.777875088576046 26.744474264101935 28.983735056285276 23.493915591036735 88-89 20.750701674393916 26.195033885501907 28.998912125186294 24.055352314917887 90-91 20.45280223513869 26.070007514623377 29.695400136682544 23.781790113555388 92-93 20.63752549576999 26.069850146120654 29.587245601311295 23.705378756798062 94-95 20.278672683544254 26.184888719521087 29.18616610704002 24.35027248989464 96-97 20.165259205534696 26.393542077027625 29.333849261982216 24.10734945545546 98-99 20.432074097625527 26.535249557356916 28.633878506263123 24.398797838754437 100-101 20.55157115444809 25.74494475734252 29.1058718774727 24.597612210736692 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 933.0 1 535.0 2 152.5 3 168.0 4 143.5 5 142.0 6 232.5 7 468.5 8 805.0 9 981.5 10 903.5 11 830.0 12 889.0 13 944.0 14 998.0 15 1047.0 16 1104.5 17 1170.5 18 1208.0 19 1272.5 20 1412.5 21 1577.0 22 1787.5 23 2058.5 24 2432.5 25 2918.5 26 3563.5 27 4277.5 28 4923.0 29 5647.5 30 6583.0 31 7669.5 32 8742.0 33 9745.0 34 10860.5 35 11713.5 36 12608.0 37 13554.0 38 14285.0 39 14759.0 40 15138.5 41 16194.0 42 17304.0 43 17972.5 44 18463.0 45 19200.0 46 19601.0 47 20356.0 48 22799.5 49 22649.0 50 19764.0 51 17836.5 52 16603.5 53 16174.0 54 15397.0 55 13738.5 56 12082.0 57 10619.0 58 9354.0 59 8151.0 60 6943.0 61 5686.5 62 4512.5 63 3508.0 64 2633.0 65 1934.0 66 1432.0 67 1124.5 68 777.0 69 499.5 70 383.0 71 285.5 72 219.5 73 170.0 74 125.5 75 96.0 76 69.5 77 49.0 78 34.5 79 21.5 80 12.5 81 12.0 82 10.5 83 5.5 84 2.0 85 1.5 86 4.5 87 3.5 88 1.0 89 2.0 90 1.5 91 0.0 92 1.5 93 1.5 94 0.5 95 0.5 96 0.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36020343638345664 2 0.3053097516788157 3 0.30123636871486703 4 0.3016243099495288 5 0.30337004550550684 6 0.26612768697797656 7 0.2641879808046677 8 0.26593371636064567 9 0.2783478358698225 10-11 0.2820332775991093 12-13 0.27252871734989587 14-15 0.26089048031004264 16-17 0.2579809210500793 18-19 0.2451788603062408 20-21 0.2501251110481784 22-23 0.24896128734419312 24-25 0.24847636080086588 26-27 0.24770047833154235 28-29 0.2664186429039729 30-31 0.2816453363644475 32-33 0.2726257026585613 34-35 0.2686493050032781 36-37 0.25371356746879986 38-39 0.2393597417863142 40-41 0.23577128536569283 42-43 0.2521618025301527 44-45 0.24828239018353498 46-47 0.26244224524868975 48-49 0.26942518747260163 50-51 0.2820332775991093 52-53 0.3059886488394738 54-55 0.2713648936459105 56-57 0.23674113845234723 58-59 0.22345415116518152 60-61 0.2254908426471558 62-63 0.2310190052410861 64-65 0.22267826869585794 66-67 0.23024312277176254 68-69 0.22966121091976985 70-71 0.23160091709307873 72-73 0.23509238820503467 74-75 0.24168738919428484 76-77 0.25739900919808667 78-79 0.25575025895077413 80-81 0.24343312475026282 82-83 0.23470444697037293 84-85 0.21870187104057479 86-87 0.22374510709117784 88-89 0.2398446683296414 90-91 0.23499540289636925 92-93 0.2407175361076304 94-95 0.23489841758770383 96-97 0.24420900721958638 98-99 0.25245275845614906 100-101 0.2279154753637919 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 515542.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.73120444118951 #Duplication Level Percentage of deduplicated Percentage of total 1 80.66438125484088 50.60173791619478 2 11.439693886548408 14.352515518833881 3 3.464665365025992 6.520278940012619 4 1.4764798009585758 3.7048542498887684 5 0.7913894409418029 2.4822406406159456 6 0.4769223804293644 1.7950749209575931 7 0.31878130998229387 1.399827486997064 8 0.2251330467383051 1.129829374512679 9 0.15906084113661664 0.89802703295358 >10 0.9072439148728166 10.195306649859496 >50 0.050935406529693014 2.2006484881338095 >100 0.022535057264063664 2.4444548167185833 >500 0.0018521964874572724 0.8497399774824598 >1k 9.260982437286362E-4 1.4254639868387389 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3187 0.618184357433536 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2436 0.47251242381804004 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1738 0.3371209329210811 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 970 0.18815149881096013 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 924 0.17922885041373932 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 735 0.14256840373820173 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 710 0.13771913830492957 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 528 0.10241648595070818 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 521 0.10105869162939198 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.698530866544336E-5 0.0 20-21 0.0 0.0 0.0 1.9397061733088673E-4 0.0 22-23 0.0 0.0 0.0 1.9397061733088673E-4 0.0 24-25 0.0 0.0 0.0 1.9397061733088673E-4 0.0 26-27 0.0 0.0 0.0 5.819118519926602E-4 0.0 28-29 0.0 0.0 0.0 0.001648750247312537 0.0 30-31 0.0 0.0 0.0 0.004364338889944952 0.0 32-33 0.0 0.0 0.0 0.007273898149908252 0.0 34-35 0.0 0.0 0.0 0.009698530866544337 0.0 36-37 0.0 0.0 0.0 0.01241411950917675 0.0 38-39 0.0 0.0 0.0 0.014935737534478277 0.0 40-41 0.0 0.0 0.0 0.02114279728906665 0.0 42-43 0.0 0.0 0.0 0.03559360828021771 0.0 44-45 0.0 0.0 0.0 0.05945199421191678 0.0 46-47 0.0 0.0 0.0 0.08088574742697976 0.0 48-49 0.0 0.0 0.0 0.13383972595831184 0.0 50-51 0.0 0.0 0.0 0.19173995523158152 0.0 52-53 0.0 0.0 0.0 0.257689965124083 0.0 54-55 0.0 0.0 0.0 0.39783373614564865 0.0 56-57 0.0 0.0 0.0 0.5296367706219862 0.0 58-59 0.0 0.0 0.0 0.6788971606581035 0.0 60-61 0.0 0.0 0.0 0.848330494896633 0.0 62-63 0.0 0.0 0.0 1.082162074089017 0.0 64-65 0.0 0.0 0.0 1.237435553262392 0.0 66-67 0.0 0.0 0.0 1.5092853734516296 0.0 68-69 0.0 0.0 0.0 1.883842635517572 0.0 70-71 0.0 0.0 0.0 2.140562747555 0.0 72-73 0.0 0.0 0.0 2.565358399509642 0.0 74-75 0.0 0.0 0.0 3.112161569765412 0.0 76-77 0.0 0.0 0.0 3.5198878073949356 0.0 78-79 0.0 0.0 0.0 3.9140361018112975 0.0 80-81 0.0 0.0 0.0 4.4809152309608145 0.0 82-83 0.0 0.0 0.0 5.172614452362756 0.0 84-85 0.0 0.0 0.0 5.8453045532662715 0.0 86-87 0.0 0.0 0.0 6.785674106086409 0.0 88-89 0.0 0.0 0.0 7.950661633775715 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTATAC 25 0.0049937326 57.074734 1 ACACGGC 25 0.0050130123 57.019203 3 GTATCAA 3400 0.0 33.153706 1 GGTATCA 2300 0.0 29.984915 1 GGGACCG 65 0.0058314977 29.240618 5 GTACATG 2185 0.0 27.209543 1 ATCAACG 4220 0.0 26.347738 3 TCAACGC 4230 0.0 26.288006 4 TATCAAC 4235 0.0 26.254416 2 CAACGCA 4285 0.0 25.948063 5 AACGCAG 4360 0.0 25.603218 6 TACATGG 2280 0.0 25.42523 2 ACATGGG 2255 0.0 25.28568 3 TGCACCG 135 1.3373541E-5 24.63793 5 GGGCGCG 120 1.7051242E-4 23.758001 7 CCGACGC 70 1.7799633E-5 23.748762 94-95 GAGTACT 2550 0.0 23.283098 12-13 AGTACTT 2540 0.0 23.187765 12-13 ACGCAGA 4795 0.0 22.990017 7 CCGTGTG 125 2.2484126E-4 22.80768 9 >>END_MODULE