FastQCFastQC Report
Thu 26 May 2016
SRR937371_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937371_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515542
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34950.677927307571449No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29040.563290672728895No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20750.40248903096158994No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT17350.33653902106908845No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6470.1254989894130837No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5330.1033863390373626No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCACCG608.286681E-539.581475
CGGATAT300.00394979831.66825138-39
TAAGGTG2104.3655746E-1127.1415825
GTAAGGT2151.6570993E-924.3011844
GTACGAG1000.001642624623.774251
GGTAAGG2202.1627784E-923.7488843
GAGTACT32550.022.83908712-13
AGTACTT33050.022.7810212-13
GTATCAA57000.022.689811
GTATAGG1703.1156724E-622.3757631
CCTTATA1703.1446853E-622.351892
AAGGTGT3007.2759576E-1222.1656276
GGTATCA43550.021.836281
GTACATG24500.021.7364581
GTACTTT34150.021.55828514-15
TACATGG25100.021.1942212
TAGACAG2309.0007234E-820.6512055
ATAGCAC1150.003721546120.6512053
CGGTTAC1058.6058026E-720.35717428-29
AGAGTAC52300.020.20894610-11
Produced by FastQC (version 0.11.5)