##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937371_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 515542 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.28810649762774 33.0 31.0 34.0 31.0 34.0 2 32.483584266655285 34.0 31.0 34.0 31.0 34.0 3 32.5410383635087 34.0 31.0 34.0 31.0 34.0 4 36.1628732479604 37.0 35.0 37.0 35.0 37.0 5 36.09648098506038 37.0 35.0 37.0 35.0 37.0 6 36.144222973104036 37.0 35.0 37.0 35.0 37.0 7 36.08242781383476 37.0 35.0 37.0 35.0 37.0 8 36.06219667844715 37.0 35.0 37.0 35.0 37.0 9 37.77449363970346 39.0 37.0 39.0 35.0 39.0 10-11 37.69503260646077 39.0 37.0 39.0 35.0 39.0 12-13 37.588086906595386 39.0 37.0 39.0 35.0 39.0 14-15 38.798562483754964 40.0 38.0 41.0 35.0 41.0 16-17 38.76108348107428 40.0 38.0 41.0 35.0 41.0 18-19 38.77996943023071 40.0 38.0 41.0 35.0 41.0 20-21 38.69382222980863 40.0 38.0 41.0 34.0 41.0 22-23 38.641550057997215 40.0 38.0 41.0 34.0 41.0 24-25 38.64723436693809 40.0 38.0 41.0 34.0 41.0 26-27 38.48741227678831 40.0 38.0 41.0 34.0 41.0 28-29 38.33768441756443 40.0 38.0 41.0 34.0 41.0 30-31 38.3554113922823 40.0 38.0 41.0 34.0 41.0 32-33 38.22652373618444 40.0 37.5 41.0 34.0 41.0 34-35 38.07833794336834 40.0 37.0 41.0 34.0 41.0 36-37 37.90837312963832 40.0 37.0 41.0 33.5 41.0 38-39 37.76628480317802 40.0 37.0 41.0 33.0 41.0 40-41 37.55955576849219 40.0 37.0 41.0 33.0 41.0 42-43 37.31848908527336 40.0 36.5 41.0 33.0 41.0 44-45 36.94470867552983 40.0 36.0 41.0 31.5 41.0 46-47 36.48147095677947 39.5 36.0 41.0 30.5 41.0 48-49 36.07305515360533 39.0 36.0 41.0 29.5 41.0 50-51 35.79433586400332 39.0 35.0 41.0 29.0 41.0 52-53 35.53886686244768 39.0 35.0 40.0 28.0 41.0 54-55 35.31595097974559 39.0 35.0 40.0 27.5 41.0 56-57 35.017759949722816 38.0 35.0 40.0 26.5 41.0 58-59 34.75264381951422 38.0 34.0 40.0 26.0 41.0 60-61 34.38808962218403 38.0 34.0 40.0 26.0 41.0 62-63 34.018465032916815 37.0 34.0 40.0 24.5 41.0 64-65 33.61499742019079 37.0 33.0 39.5 23.0 41.0 66-67 33.190011677031166 36.0 33.0 39.0 22.0 40.5 68-69 32.725507330149625 36.0 32.0 39.0 21.5 40.0 70-71 32.270447412625934 35.0 32.0 38.0 19.5 40.0 72-73 31.786263776763096 35.0 31.0 37.5 18.0 39.5 74-75 31.327534905012588 35.0 31.0 37.0 15.5 39.0 76-77 30.006607609079378 33.5 29.5 35.5 13.0 38.0 78-79 30.641198001326757 34.0 31.0 36.0 12.0 38.0 80-81 30.46862913205908 34.0 31.0 36.0 7.5 37.0 82-83 30.091349492378896 34.0 30.5 35.0 6.5 37.0 84-85 29.705358632274383 34.0 30.0 35.0 2.0 36.5 86-87 29.364635664989468 34.0 30.0 35.0 2.0 36.0 88-89 28.950365828584285 34.0 29.0 35.0 2.0 36.0 90-91 28.45026690356945 34.0 29.0 35.0 2.0 35.0 92-93 27.981497142812806 33.5 28.0 35.0 2.0 35.0 94-95 27.51487754634928 33.0 27.0 35.0 2.0 35.0 96-97 26.902625780246808 33.0 25.5 35.0 2.0 35.0 98-99 26.01542163393089 32.5 24.5 34.0 2.0 35.0 100-101 23.212432740688442 29.5 10.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07886000888920108 1101 2 0.1376155560568435 1101 3 0.10134019456592114 1101 4 0.024047795501900282 1101 5 0.11929742371678742 1101 6 0.06732441277348755 1101 7 0.09257123765679864 1101 8 -0.008852253492761974 1101 9 0.09859716030030796 1101 10-11 -0.07373240821764426 1101 12-13 0.013830599930784615 1101 14-15 0.08857945502413145 1101 16-17 0.08584509005220298 1101 18-19 0.17159136585132728 1101 20-21 0.05794717247270853 1101 22-23 0.12335394722154547 1101 24-25 0.09108804063873066 1101 26-27 0.013107263510370615 1101 28-29 -0.12129511712366536 1101 30-31 0.07684727894906018 1101 32-33 0.06420380289397087 1101 34-35 0.10595448053832257 1101 36-37 0.2579052159611379 1101 38-39 0.3147634746706487 1101 40-41 0.26432217411393566 1101 42-43 0.5770458316329581 1101 44-45 0.7489420901896437 1101 46-47 0.9037795647596667 1101 48-49 0.9406228079186718 1101 50-51 1.2180347985172304 1101 52-53 1.1637303158504153 1101 54-55 0.9582565670265595 1101 56-57 1.1164441996377263 1101 58-59 0.9593238640381401 1101 60-61 0.8540052249639345 1101 62-63 1.1524997965086925 1101 64-65 0.8471617029969067 1101 66-67 0.7957036954271928 1101 68-69 0.9471554111643243 1101 70-71 1.003097069400937 1101 72-73 1.0749312805182534 1101 74-75 1.075398314312448 1101 76-77 1.0151163508021135 1101 78-79 1.044093332162145 1101 80-81 0.9715054301797537 1101 82-83 0.8244706096568208 1101 84-85 0.96964663410699 1101 86-87 1.0556867378150692 1101 88-89 1.3614179002487496 1101 90-91 1.3218483868343753 1101 92-93 1.3239123838322655 1101 94-95 1.4019703379033892 1101 96-97 1.41419034439539 1101 98-99 1.4639624523454344 1101 100-101 2.114506965032909 1102 1 -0.019278787463058222 1102 2 0.04268204398379538 1102 3 -0.004095230461267363 1102 4 0.04920311859397941 1102 5 -0.06642574169478621 1102 6 -0.039836346984870374 1102 7 -0.028365465378435317 1102 8 -0.039796566333087924 1102 9 0.0164809661194667 1102 10-11 -0.01232882406628022 1102 12-13 -0.038150957033437294 1102 14-15 -0.024703044429237764 1102 16-17 0.10701839405389535 1102 18-19 0.1559120164944403 1102 20-21 0.013084172834581409 1102 22-23 -0.0780108527035992 1102 24-25 -0.08990167323450748 1102 26-27 -0.05195895707085896 1102 28-29 -0.15858363949429588 1102 30-31 -0.22675052614253843 1102 32-33 -0.12282410513527964 1102 34-35 -0.030236683112583762 1102 36-37 -0.16107948136053807 1102 38-39 -0.1955546380976827 1102 40-41 -0.2228652820246566 1102 42-43 -0.3587993102655531 1102 44-45 -0.26644843030383925 1102 46-47 -0.20900882250590058 1102 48-49 -0.12847030764247336 1102 50-51 0.02684250342101535 1102 52-53 -0.1999448476362673 1102 54-55 -0.14092774207093584 1102 56-57 -0.1673055724023058 1102 58-59 -0.12332033794918829 1102 60-61 -0.11500319929081115 1102 62-63 -0.22232845773327625 1102 64-65 -0.08719967012893193 1102 66-67 -0.050733875049431276 1102 68-69 -0.005230315247438 1102 70-71 -0.1353536430276776 1102 72-73 -0.006873646854419491 1102 74-75 -0.11739662292336916 1102 76-77 -0.0762595535186712 1102 78-79 0.04411290568842574 1102 80-81 0.06923318248011867 1102 82-83 0.21589960120010332 1102 84-85 0.31407290540752086 1102 86-87 0.2814649764459567 1102 88-89 0.5058910525317408 1102 90-91 0.6382954958245435 1102 92-93 0.6717372876594112 1102 94-95 0.711798253189162 1102 96-97 0.9946208283007003 1102 98-99 1.2430575443553273 1102 100-101 2.186541838373099 1103 1 -0.02416931886555318 1103 2 0.015054506855413763 1103 3 -0.07049939455048815 1103 4 -0.05393157985849939 1103 5 -0.10918616910079493 1103 6 -0.21256991612112586 1103 7 -0.014564914466681955 1103 8 -0.032286296217144184 1103 9 -0.033488368926867906 1103 10-11 -0.09669709958560446 1103 12-13 -0.11578748216547297 1103 14-15 0.009971484097100358 1103 16-17 -0.046552222576025315 1103 18-19 -0.06776106337719057 1103 20-21 -0.1253244746823512 1103 22-23 -0.06686242375376139 1103 24-25 -0.13553826181128414 1103 26-27 -0.26439504972131544 1103 28-29 -0.07017604938955913 1103 30-31 -0.1111866082188726 1103 32-33 -0.0987851606441339 1103 34-35 0.08671442743457192 1103 36-37 -0.10868668973947138 1103 38-39 -0.037716041495144736 1103 40-41 -0.1174716867703296 1103 42-43 -0.08941297943300697 1103 44-45 -0.1627645383925156 1103 46-47 -0.03984798482395746 1103 48-49 0.04506689476207981 1103 50-51 -0.10886842123307616 1103 52-53 -0.2025665689975824 1103 54-55 0.028481587476868242 1103 56-57 0.07555028645431605 1103 58-59 -0.13903877022986677 1103 60-61 -0.05997943395172456 1103 62-63 0.08786340970953432 1103 64-65 -0.04347104968174875 1103 66-67 -0.15435564399429325 1103 68-69 -0.17351482257266326 1103 70-71 -0.30377825746875686 1103 72-73 -0.3708871341469653 1103 74-75 -0.5023111840180015 1103 76-77 -0.4297398935824326 1103 78-79 -0.20659225606920373 1103 80-81 -0.24937634691858435 1103 82-83 -0.05713215817517536 1103 84-85 -0.1181304508187857 1103 86-87 0.07509776891295061 1103 88-89 0.31721170749285577 1103 90-91 0.5027716009524639 1103 92-93 0.43903589028919754 1103 94-95 0.32007858373621545 1103 96-97 0.4881316218356808 1103 98-99 0.7300209737545593 1103 100-101 1.298724019920197 1104 1 -0.0023717783780838886 1104 2 -0.022207255549183458 1104 3 0.059130645161928896 1104 4 -0.022346183398660457 1104 5 -0.17902613839379455 1104 6 -0.01680541518367562 1104 7 0.041264986473720455 1104 8 0.029895377019052205 1104 9 0.0688449201175203 1104 10-11 -0.0055024792156430635 1104 12-13 -0.06374797643005792 1104 14-15 -0.15830988354539954 1104 16-17 -0.04809864476774095 1104 18-19 -0.02634721274903029 1104 20-21 -0.04719878266764255 1104 22-23 0.004249007221915235 1104 24-25 -0.039951654787053315 1104 26-27 0.21336267500814898 1104 28-29 -0.016483089854887112 1104 30-31 0.04044580833338074 1104 32-33 -0.09292425744499155 1104 34-35 -0.14747811135516287 1104 36-37 -0.13298101927871642 1104 38-39 0.005011714384210109 1104 40-41 0.013734301094501689 1104 42-43 -0.15863659429637522 1104 44-45 -0.09534754324953099 1104 46-47 -0.0046788748840427274 1104 48-49 -0.2178876204954534 1104 50-51 -0.3197243788337971 1104 52-53 -0.39250153806914057 1104 54-55 -0.12960778760617586 1104 56-57 -0.30317182996655134 1104 58-59 -0.5027063374383332 1104 60-61 -0.20798938420048074 1104 62-63 -0.145022605319177 1104 64-65 -0.2880364275402272 1104 66-67 -0.13229052448797063 1104 68-69 -0.08469704203911732 1104 70-71 -0.1399387015392044 1104 72-73 -0.07758177666140043 1104 74-75 -0.14932647846564961 1104 76-77 -0.14821170367145342 1104 78-79 -0.18356016134992004 1104 80-81 -0.10496671850279782 1104 82-83 -0.022335813489110734 1104 84-85 -0.19401401519276007 1104 86-87 -0.026072935428842925 1104 88-89 0.04298934748281624 1104 90-91 0.03509699180236936 1104 92-93 0.04543453504760464 1104 94-95 0.27256762222330977 1104 96-97 0.3566654364421744 1104 98-99 0.5672628061356804 1104 100-101 1.1564125800867266 1105 1 -0.10913714986761391 1105 2 0.002590648138522056 1105 3 -0.002924963043106743 1105 4 0.0644555421232198 1105 5 0.013922111485804578 1105 6 -0.02075412596197168 1105 7 -0.3203592412620182 1105 8 0.029095277589220814 1105 9 0.08554343540227194 1105 10-11 0.00934337865148649 1105 12-13 0.009326476835326503 1105 14-15 -0.015923256857810486 1105 16-17 -0.11362523447986206 1105 18-19 -0.05876807022775665 1105 20-21 0.23649860885284824 1105 22-23 -0.0720426743333249 1105 24-25 0.008108686692274603 1105 26-27 0.005124499131277105 1105 28-29 -0.14682161736121913 1105 30-31 -0.10420906139800223 1105 32-33 0.20509465493763912 1105 34-35 0.17850026265636387 1105 36-37 0.3011001092498802 1105 38-39 0.29127382592169937 1105 40-41 0.0718891009142908 1105 42-43 0.027385228480312662 1105 44-45 0.15609600183826444 1105 46-47 0.05215925645655517 1105 48-49 0.12968308084736435 1105 50-51 0.12870027976455134 1105 52-53 -0.006892452668232352 1105 54-55 -0.14273692727919496 1105 56-57 -0.07638636536511711 1105 58-59 0.046108790396317545 1105 60-61 0.08694953377509052 1105 62-63 0.1406361949977537 1105 64-65 0.22708389809246654 1105 66-67 0.29932816210609303 1105 68-69 0.34965467027722497 1105 70-71 0.3092951191038793 1105 72-73 0.2671432636025024 1105 74-75 0.3618464310480256 1105 76-77 0.13417808198476067 1105 78-79 0.5334998228034493 1105 80-81 0.5058755350329172 1105 82-83 0.3217587277095362 1105 84-85 0.4911041354123604 1105 86-87 0.7477293752790253 1105 88-89 0.7756655117698976 1105 90-91 1.0168992610811394 1105 92-93 0.6064091027608711 1105 94-95 0.5324135059836195 1105 96-97 1.0065636762234256 1105 98-99 0.8709329062387354 1105 100-101 1.4390108058856583 1106 1 0.06162069270189363 1106 2 0.03027748901010341 1106 3 -0.059007755679381546 1106 4 0.04792931880843554 1106 5 -0.05620895584427643 1106 6 -0.011992666926914808 1106 7 0.045049541470341126 1106 8 0.04239110683531777 1106 9 0.10686797576052953 1106 10-11 -0.10521545588537151 1106 12-13 -0.31383505468662065 1106 14-15 -0.20393219165507048 1106 16-17 -0.155976882571764 1106 18-19 0.06105591269054855 1106 20-21 0.06876433634153756 1106 22-23 -0.05566418245462046 1106 24-25 0.10991337391527622 1106 26-27 0.14066907659967143 1106 28-29 0.10763691737930259 1106 30-31 0.2402770104822025 1106 32-33 0.28964234067545647 1106 34-35 -0.11701579279610286 1106 36-37 0.49048590103128475 1106 38-39 0.4460530363661164 1106 40-41 0.4760645822233087 1106 42-43 0.4800422276017997 1106 44-45 0.699867841468901 1106 46-47 0.5830055803074927 1106 48-49 0.3357073718349568 1106 50-51 0.2952172452426751 1106 52-53 0.3484607436491203 1106 54-55 0.3441464909504717 1106 56-57 0.42471866577666617 1106 58-59 0.3597820242177576 1106 60-61 0.21187517561893543 1106 62-63 0.4428828946774743 1106 64-65 0.3249057457990858 1106 66-67 0.3294653750639185 1106 68-69 0.27826690086433103 1106 70-71 0.5443456826500608 1106 72-73 0.5191336984844632 1106 74-75 0.5779913102825027 1106 76-77 0.5993233118351746 1106 78-79 0.672857841074876 1106 80-81 0.4980083436241891 1106 82-83 0.6438999274463768 1106 84-85 0.7011605097283642 1106 86-87 0.7855466531598871 1106 88-89 1.1837312542287002 1106 90-91 1.0013776115316233 1106 92-93 0.9528452295971732 1106 94-95 0.504379988675403 1106 96-97 0.6697419112464154 1106 98-99 1.0326023108613498 1106 100-101 1.5785579203952516 1107 1 -0.21303050492609543 1107 2 -0.20138617304030504 1107 3 -0.12820002184878376 1107 4 -0.11871065928971802 1107 5 -0.2045474633162172 1107 6 -0.29623625263079845 1107 7 -0.17539975627593662 1107 8 -0.16680250237172345 1107 9 -0.14912016418399787 1107 10-11 -0.26768426900168407 1107 12-13 -0.13259987329905698 1107 14-15 -0.19827077144441319 1107 16-17 -0.02060643663160988 1107 18-19 0.004198930702756343 1107 20-21 -0.19855955784775858 1107 22-23 -0.18942040046061948 1107 24-25 -0.28119726655871347 1107 26-27 -0.32433704455265655 1107 28-29 -0.23038757756106065 1107 30-31 -0.13172891954553023 1107 32-33 -0.2517645429090152 1107 34-35 -0.27137214200654824 1107 36-37 -0.3195104314428576 1107 38-39 -0.4110296792559964 1107 40-41 -0.304457239555596 1107 42-43 0.024439952702650203 1107 44-45 -0.06868544871504412 1107 46-47 -0.2695547143342978 1107 48-49 -0.074267781968679 1107 50-51 -0.3035244317981025 1107 52-53 -0.012625257249680999 1107 54-55 0.05975827042369275 1107 56-57 -0.06387842809650124 1107 58-59 -0.03287052677892888 1107 60-61 0.3721021489802965 1107 62-63 -0.005451480370957995 1107 64-65 -0.031293664297457724 1107 66-67 -0.03616370893276155 1107 68-69 0.2580516015993908 1107 70-71 0.2822105627323026 1107 72-73 0.0807662123926427 1107 74-75 0.2249919456426177 1107 76-77 0.10258276087980889 1107 78-79 0.19600716103292726 1107 80-81 0.14528791757132353 1107 82-83 0.2698200373688415 1107 84-85 0.17257705285278746 1107 86-87 0.5270713108144278 1107 88-89 0.7785548772277018 1107 90-91 0.8158757028685102 1107 92-93 0.5927510103411109 1107 94-95 0.24897813631656618 1107 96-97 0.6202613052265029 1107 98-99 0.7582636277614121 1107 100-101 1.7966491539753235 1108 1 -0.1466566128538318 1108 2 0.04417440601191913 1108 3 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0.34860276544810986 2316 90-91 0.4293185022077779 2316 92-93 0.36017110823918586 2316 94-95 0.35173322443998245 2316 96-97 0.6361621644866631 2316 98-99 0.7062079198189402 2316 100-101 1.4922596530130114 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 2.0 4 3.0 5 20.0 6 18.0 7 42.0 8 125.0 9 231.0 10 423.0 11 703.0 12 1199.0 13 1822.0 14 2248.0 15 3187.0 16 4734.0 17 6907.0 18 6151.0 19 3367.0 20 2907.0 21 2917.0 22 2847.0 23 3044.0 24 3544.0 25 4036.0 26 4873.0 27 5852.0 28 7138.0 29 9000.0 30 11170.0 31 14131.0 32 18226.0 33 24207.0 34 34450.0 35 50244.0 36 77517.0 37 114980.0 38 86544.0 39 6732.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49809635065958 20.083791619285655 15.510181904270306 20.907930125784453 2 15.761276481838532 21.762727382056166 39.64643035872926 22.829565777376043 3 19.10998521943896 26.085944501126967 31.06439436554151 23.73967591389256 4 12.864519282619069 17.75199692750542 37.8380422933534 31.54544149652211 5 13.132933490580678 38.791277427764506 33.876586698116135 14.199202383538687 6 30.697983869403462 37.74008713160131 17.20499978663232 14.356929212362912 7 27.46158411923762 34.58825857059173 20.795395913427033 17.15476139674362 8 26.541969422471883 34.695718292593035 19.823797091216623 18.938515193718455 9 26.890534621815487 16.77419104554042 20.666017511667334 35.669256820976756 10-11 24.721361208204183 27.430742791082007 27.19565040287697 20.652245597836842 12-13 26.377195909246097 24.922018063875353 27.942460194295293 20.758325832583257 14-15 22.881646888129385 26.134340170151027 27.382056166131953 23.601956775587634 16-17 21.409797843822616 29.467531258364982 27.781732623142247 21.340938274670155 18-19 21.307090401945914 27.484860593317322 31.054307893440303 20.153741111296462 20-21 22.183546636355526 26.509964270612286 31.1098804753056 20.196608617726586 22-23 21.803111903812823 26.3931845756799 31.209914331962917 20.593789188544363 24-25 22.006645433349757 26.599869651745156 31.122973491975436 20.270511422929655 26-27 21.610698809389874 26.909282413963 31.23072387078253 20.2492949058646 28-29 21.22504124432254 27.41299896901317 31.017088388450233 20.344871398214057 30-31 21.518851071010843 26.830843715630763 30.925862346544697 20.724442866813696 32-33 21.242789142300726 27.080334870873763 30.8115536658507 20.86532232097482 34-35 21.789495327247828 26.833022333776878 30.81378432795 20.563698011025288 36-37 22.021678156192902 26.55603229222837 30.70661556187469 20.71567398970404 38-39 21.584953311272407 27.02117383258784 30.885456471053764 20.508416385085987 40-41 21.582528678555775 27.027962804194424 30.61506143049451 20.7744470867553 42-43 21.65421235188677 27.334796036346425 29.990940552956058 21.020051058810747 44-45 21.85117798355905 27.36382292810285 29.835299548824345 20.949699539513755 46-47 22.046894336445916 27.855538443036647 29.17017430199673 20.927392918520702 48-49 22.415729465300597 27.945249853552184 28.661195402120487 20.97782527902673 50-51 22.14552839535867 28.007514421715396 28.785142626594922 21.061814556331008 52-53 22.529127141073996 28.366096618797904 28.14448497356663 20.96029126656147 54-55 21.979004620380106 28.14077223582172 28.40253558390975 21.477687559888427 56-57 22.16919281067304 28.32766292562003 28.293815052895788 21.209329210811145 58-59 22.415535494683265 27.909074333419976 28.323007630804085 21.352382541092677 60-61 22.26433539847384 27.679025181265544 29.08657296592712 20.970066454333498 62-63 21.859712690721608 28.004701847764103 29.046033106904968 21.089552354609324 64-65 21.883086150109982 27.98685655096966 28.860112270193312 21.26994502872705 66-67 21.810541139228228 27.97104794565719 28.523379278506894 21.695031636607688 68-69 21.829841215652653 27.990735963316276 28.679137684223594 21.500285136807477 70-71 22.03845661459202 28.20012724472497 28.368202784642182 21.393213356040828 72-73 21.901631585430074 27.816092399932884 28.22750447830097 22.054771536336066 74-75 21.820627611329435 28.25754254745491 27.927792497992403 21.994037343223248 76-77 21.87076901590947 27.92701661552308 28.220009233001385 21.982205135566065 78-79 21.882698208875322 27.79511659573808 28.132625469893817 22.18955972549278 80-81 21.638877142890394 28.305938216478964 28.12447870396592 21.93070593666471 82-83 21.768791273590622 28.079183222979466 28.09150044080122 22.060525062628688 84-85 21.615039720371687 28.084741965486774 27.997358117629716 22.302860196511823 86-87 21.576127648183853 27.8882224920569 27.684844299785468 22.850805559973775 88-89 21.581655810777782 27.409599993792938 27.857575134518626 23.151169060910654 90-91 21.601488312902656 27.42767462748529 28.232841820195816 22.737995239416236 92-93 21.64922357131011 27.190376571066672 28.145503638463843 23.014896219159375 94-95 21.300689371573995 27.616178701250334 27.850980133529372 23.2321517936463 96-97 21.282553118853556 27.763596370421805 27.944667941700192 23.009182569024446 98-99 21.335326077468736 28.069857255638297 27.186409510859548 23.408407156033416 100-101 21.198053425081405 27.813963774772322 27.192006510569488 23.795976289576785 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 171.0 1 125.0 2 101.0 3 128.5 4 124.0 5 137.0 6 229.0 7 482.0 8 809.0 9 998.0 10 976.0 11 899.0 12 985.0 13 1096.5 14 1145.0 15 1245.0 16 1294.5 17 1331.5 18 1376.5 19 1423.0 20 1510.0 21 1591.0 22 1807.5 23 2149.0 24 2478.5 25 2913.5 26 3512.0 27 4264.0 28 4996.0 29 5728.5 30 6591.0 31 7480.0 32 8617.0 33 9712.0 34 10731.0 35 11578.5 36 12321.5 37 13367.5 38 14489.0 39 14828.0 40 15036.5 41 16015.0 42 17029.0 43 18129.0 44 18802.5 45 19500.5 46 19699.5 47 20208.0 48 22994.0 49 22588.0 50 19621.0 51 18124.5 52 16921.0 53 16217.0 54 15238.0 55 13621.5 56 11860.0 57 10350.5 58 9385.5 59 8328.0 60 7051.0 61 5717.0 62 4491.0 63 3464.0 64 2522.0 65 1906.5 66 1402.5 67 1122.5 68 807.5 69 447.0 70 324.0 71 236.0 72 178.0 73 145.5 74 113.5 75 100.0 76 70.5 77 48.0 78 30.0 79 14.5 80 8.0 81 6.5 82 4.5 83 3.0 84 2.0 85 0.5 86 0.0 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04189765334347153 2 0.0 3 0.0 4 0.0 5 0.0011638237039853204 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0073708834585736955 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.001066838395319877 24-25 0.0 26-27 0.0015517649386470938 28-29 0.0032005151859596306 30-31 1.9397061733088673E-4 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01125029580519143 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 9.698530866544336E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 9.698530866544336E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 6.788971606581035E-4 84-85 9.698530866544336E-5 86-87 0.0 88-89 0.0 90-91 0.011444266422522316 92-93 0.001648750247312537 94-95 0.0 96-97 0.0 98-99 9.698530866544335E-4 100-101 0.014062869756489288 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 515542.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.33872475816791 #Duplication Level Percentage of deduplicated Percentage of total 1 79.44422901117508 47.93563467930152 2 11.812116946674069 14.25456146513315 3 3.8192266250677447 6.9134179235708775 4 1.6895234578119565 4.077747635735351 5 0.844600550799951 2.5481060082657634 6 0.5105497987580633 1.848355426956048 7 0.34616800566393346 1.4621135209687997 8 0.26148515577833825 1.262214467428465 9 0.18760529234656587 1.018787768826756 >10 0.9957709842972461 10.787686926699742 >50 0.0578635910491401 2.3976324463485055 >100 0.028931794291821657 3.2846457814770496 >500 6.429287620404796E-4 0.22888165951003384 >1k 0.0012858575240809592 1.9802142897779114 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3495 0.677927307571449 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2904 0.563290672728895 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2075 0.40248903096158994 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1735 0.33653902106908845 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 647 0.1254989894130837 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 533 0.1033863390373626 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 9.698530866544336E-5 0.0 20-21 0.0 0.0 0.0 1.9397061733088673E-4 0.0 22-23 0.0 0.0 0.0 1.9397061733088673E-4 0.0 24-25 0.0 0.0 0.0 1.9397061733088673E-4 0.0 26-27 0.0 0.0 0.0 5.819118519926602E-4 0.0 28-29 0.0 0.0 0.0 0.001648750247312537 0.0 30-31 0.0 0.0 0.0 0.004267353581279508 0.0 32-33 0.0 0.0 0.0 0.007079927532577366 0.0 34-35 0.0 0.0 0.0 0.009504560249213449 0.0 36-37 0.0 0.0 0.0 0.012608090126507637 0.0 38-39 0.0 0.0 0.0 0.01532367876914005 0.0 40-41 0.0 0.0 0.0 0.021530738523728426 0.0 42-43 0.0 0.0 0.0 0.0357875788975486 0.0 44-45 0.0 0.0 0.0 0.059742950137913114 0.0 46-47 0.0 0.0 0.0 0.0811767033529761 0.0 48-49 0.0 0.0 0.0 0.13451862311896995 0.0 50-51 0.0 0.0 0.0 0.1934856907875595 0.0 52-53 0.0 0.0 0.0 0.26001761253205363 0.0 54-55 0.0 0.0 0.0 0.40161616318360094 0.0 56-57 0.0 0.0 0.0 0.5346800066725892 0.0 58-59 0.0 0.0 0.0 0.6851042204126918 0.0 60-61 0.0 0.0 0.0 0.8554104224292104 0.0 62-63 0.0 0.0 0.0 1.091569649029565 0.0 64-65 0.0 0.0 0.0 1.2498496727715684 0.0 66-67 0.0 0.0 0.0 1.5239301550601114 0.0 68-69 0.0 0.0 0.0 1.9053733740413001 0.0 70-71 0.0 0.0 0.0 2.163645251017376 0.0 72-73 0.0 0.0 0.0 2.592417300627301 0.0 74-75 0.0 0.0 0.0 3.14668833965031 0.0 76-77 0.0 0.0 0.0 3.5611065635777495 0.0 78-79 0.0 0.0 0.0 3.961461917748699 0.0 80-81 0.0 0.0 0.0 4.536972739369441 0.0 82-83 0.0 0.0 0.0 5.242152918675879 0.0 84-85 0.0 0.0 0.0 5.929099859953214 0.0 86-87 0.0 0.0 0.0 6.890127283519092 0.0 88-89 0.0 0.0 0.0 8.07955510899209 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TGCACCG 60 8.286681E-5 39.58147 5 CGGATAT 30 0.003949798 31.668251 38-39 TAAGGTG 210 4.3655746E-11 27.141582 5 GTAAGGT 215 1.6570993E-9 24.301184 4 GTACGAG 100 0.0016426246 23.77425 1 GGTAAGG 220 2.1627784E-9 23.748884 3 GAGTACT 3255 0.0 22.839087 12-13 AGTACTT 3305 0.0 22.78102 12-13 GTATCAA 5700 0.0 22.68981 1 GTATAGG 170 3.1156724E-6 22.375763 1 CCTTATA 170 3.1446853E-6 22.35189 2 AAGGTGT 300 7.2759576E-12 22.165627 6 GGTATCA 4355 0.0 21.83628 1 GTACATG 2450 0.0 21.736458 1 GTACTTT 3415 0.0 21.558285 14-15 TACATGG 2510 0.0 21.194221 2 TAGACAG 230 9.0007234E-8 20.651205 5 ATAGCAC 115 0.0037215461 20.651205 3 CGGTTAC 105 8.6058026E-7 20.357174 28-29 AGAGTAC 5230 0.0 20.208946 10-11 >>END_MODULE