FastQCFastQC Report
Thu 26 May 2016
SRR937370_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937370_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462164
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29970.6484711054950191No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22500.48684016929055485No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15910.3442500930405657No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT8150.17634432798746766No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN7250.15687072121584544No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6980.1510286391843588No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT6070.13133865900416303No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5130.11099955859824652No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGCT405.380373E-447.507927
TACGGGA459.585526E-442.252192
CACGGCG500.001613488438.0063367
GTATCAA29800.032.7100261
GGTATCA20200.029.4240131
GTATTAC650.005811006329.261051
GGCTCGT650.00583882529.2324736
GGGCGTA700.00837962627.1473839
CGGGTAG350.00839722527.13854860-61
TATCAAC37750.025.8130132
ATCAACG37800.025.6531163
TCAACGC37800.025.6531164
CAACGCA38350.025.2879565
GTACATG21150.024.7300831
AACGCAG39800.024.3481526
TACATGG21950.023.6044452
GCACCTA1050.002193496622.6203656
ACATGGG22100.022.583893
GAGTACT21850.022.3950412-13
ATACGTC1703.119958E-622.3712375
Produced by FastQC (version 0.11.5)