##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937370_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 462164 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.849116330999387 33.0 31.0 34.0 30.0 34.0 2 31.95990384365723 33.0 31.0 34.0 30.0 34.0 3 31.984724037354706 34.0 31.0 34.0 30.0 34.0 4 35.60654875758389 37.0 35.0 37.0 33.0 37.0 5 35.61218095740906 37.0 35.0 37.0 33.0 37.0 6 35.656816627863705 37.0 35.0 37.0 33.0 37.0 7 35.6052115699189 37.0 35.0 37.0 33.0 37.0 8 35.60301970729005 37.0 35.0 37.0 33.0 37.0 9 37.25393150483378 39.0 37.0 39.0 34.0 39.0 10-11 37.15055802702071 39.0 37.0 39.0 34.0 39.0 12-13 37.01663586951818 39.0 37.0 39.0 33.0 39.0 14-15 38.21004665010689 40.0 38.0 41.0 33.0 41.0 16-17 38.19011541357614 40.0 38.0 41.0 33.0 41.0 18-19 38.19576925074216 40.0 38.0 41.0 33.5 41.0 20-21 38.14694675483162 40.0 38.0 41.0 34.0 41.0 22-23 38.009380869128705 40.0 38.0 41.0 33.0 41.0 24-25 37.8464365463342 40.0 38.0 41.0 33.0 41.0 26-27 37.696059191109654 40.0 37.0 41.0 33.0 41.0 28-29 37.725379951705456 40.0 37.0 41.0 33.0 41.0 30-31 37.53383430989865 40.0 37.0 41.0 33.0 41.0 32-33 37.3052996338962 40.0 36.5 41.0 32.0 41.0 34-35 37.17990258869146 39.5 36.0 41.0 32.0 41.0 36-37 37.17884776832466 40.0 36.0 41.0 32.0 41.0 38-39 37.092432556408546 40.0 36.0 41.0 31.5 41.0 40-41 36.89712093542552 40.0 36.0 41.0 31.0 41.0 42-43 36.627910222345314 39.0 36.0 41.0 30.5 41.0 44-45 36.35613332063943 39.0 35.5 41.0 30.0 41.0 46-47 35.92493465523061 39.0 35.0 41.0 28.5 41.0 48-49 35.535695121212385 39.0 35.0 41.0 27.0 41.0 50-51 34.618913416016824 38.0 34.0 40.0 25.0 40.5 52-53 34.67515751984144 38.5 34.5 40.0 25.5 40.5 54-55 34.986472334495986 39.0 35.0 40.0 26.0 41.0 56-57 34.80147956136783 39.0 35.0 40.0 24.5 41.0 58-59 34.565441055556036 38.0 34.0 40.0 24.0 41.0 60-61 34.280538942886075 38.0 34.0 40.0 23.0 41.0 62-63 33.95472061865485 38.0 34.0 40.0 22.0 41.0 64-65 33.58447109684008 37.0 33.5 40.0 21.0 41.0 66-67 33.20429977237518 36.5 33.0 39.5 19.5 41.0 68-69 32.73464722479466 36.0 32.5 39.0 17.5 41.0 70-71 32.28750833037623 35.5 32.0 39.0 13.0 40.0 72-73 31.88509922884517 35.0 32.0 38.0 9.5 40.0 74-75 31.450207069352004 35.0 31.0 37.5 7.0 39.5 76-77 31.020318544932103 35.0 31.0 37.0 4.5 39.0 78-79 30.55411066201608 35.0 31.0 36.0 2.0 39.0 80-81 30.086087406202125 34.5 30.0 36.0 2.0 37.5 82-83 29.627565106758638 34.0 30.0 35.5 2.0 37.0 84-85 29.21770843250448 34.0 29.0 35.0 2.0 37.0 86-87 28.819277356090048 34.0 29.0 35.0 2.0 36.0 88-89 28.447054508789087 34.0 29.0 35.0 2.0 36.0 90-91 28.0546310400637 34.0 28.5 35.0 2.0 35.5 92-93 27.655053184583828 34.0 27.0 35.0 2.0 35.0 94-95 27.16092447702547 33.5 26.0 35.0 2.0 35.0 96-97 26.57525791710302 33.0 25.0 35.0 2.0 35.0 98-99 25.874829065007226 33.0 24.5 35.0 2.0 35.0 100-101 24.05924736673562 30.5 10.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.04487345934444065 1101 2 -0.1744371357031511 1101 3 -0.12425634517718365 1101 4 0.03352121315300849 1101 5 -0.012217790492897507 1101 6 -0.03448101451441232 1101 7 0.016478927378585695 1101 8 0.016221589905370593 1101 9 0.11857906576283028 1101 10-11 0.024082758150058226 1101 12-13 -0.09013161773733458 1101 14-15 -0.04498779197407998 1101 16-17 0.20023591080779823 1101 18-19 0.15747166061596118 1101 20-21 0.10281806300699259 1101 22-23 0.06125092177668279 1101 24-25 0.05429087583400616 1101 26-27 0.14598415535302678 1101 28-29 -0.035376784328327915 1101 30-31 0.07513363728897104 1101 32-33 -0.09767030266838361 1101 34-35 -0.08430865861880221 1101 36-37 -0.037847055734516744 1101 38-39 0.11065267393961875 1101 40-41 0.07988395057244446 1101 42-43 0.2197591504878318 1101 44-45 0.3440552189938799 1101 46-47 0.589154650573434 1101 48-49 0.5843655279196582 1101 50-51 0.2716547449869182 1101 52-53 0.27632070909771755 1101 54-55 0.4357904272469497 1101 56-57 0.31827844664852734 1101 58-59 0.5311352255672261 1101 60-61 0.42138886914827367 1101 62-63 0.27313174599652257 1101 64-65 0.27369441515290305 1101 66-67 0.26136429611519674 1101 68-69 0.5874592301462016 1101 70-71 0.6453325859444803 1101 72-73 0.49656478097488943 1101 74-75 0.439913060447374 1101 76-77 0.469271849039437 1101 78-79 0.45749502515683105 1101 80-81 0.39439244526644757 1101 82-83 0.2692692017096263 1101 84-85 0.29454057615444995 1101 86-87 0.3433397967409526 1101 88-89 0.24662547518398625 1101 90-91 0.403956964812604 1101 92-93 0.6852462053947903 1101 94-95 0.5755361647829851 1101 96-97 0.3837910529330131 1101 98-99 0.19218468810496248 1101 100-101 0.6234624571147513 1102 1 -0.31229857555848284 1102 2 -0.368140606396242 1102 3 -0.08325288496957128 1102 4 -0.10154571914576138 1102 5 -0.04068061947476309 1102 6 -0.3466109645336388 1102 7 -0.16881344268887233 1102 8 -0.1159863021641101 1102 9 -0.2899019380468886 1102 10-11 -0.0902823461804445 1102 12-13 -0.2339836326966136 1102 14-15 -0.20812522163400615 1102 16-17 -0.17295097535486548 1102 18-19 -0.1472747655837523 1102 20-21 -0.3847784907660312 1102 22-23 -0.20910838058954084 1102 24-25 -0.26900173889459467 1102 26-27 -0.35232069243279085 1102 28-29 -0.24050767804053663 1102 30-31 -0.27761530542537116 1102 32-33 -0.3772376019588606 1102 34-35 0.1033639148669181 1102 36-37 0.21025070386843936 1102 38-39 0.15625925881955993 1102 40-41 0.2604919335160716 1102 42-43 0.4212227484047162 1102 44-45 0.3630924773909996 1102 46-47 0.5372829928194207 1102 48-49 0.6123268015556675 1102 50-51 0.6293292408102076 1102 52-53 0.5578601519693436 1102 54-55 0.5941412363763092 1102 56-57 0.6361858948327992 1102 58-59 0.47849026383921966 1102 60-61 0.5870821339764873 1102 62-63 0.488226464810964 1102 64-65 0.5967030481961331 1102 66-67 0.75270067023704 1102 68-69 0.926019574069862 1102 70-71 0.9365876976148186 1102 72-73 0.5834500698497997 1102 74-75 0.684319931930375 1102 76-77 0.8315459743758424 1102 78-79 0.5617634883353304 1102 80-81 0.8621155238026752 1102 82-83 0.7374344178852219 1102 84-85 0.6747396254711511 1102 86-87 0.6109920983032886 1102 88-89 0.4515588924011418 1102 90-91 0.44086934117134646 1102 92-93 0.4601238748226315 1102 94-95 0.5482293597080137 1102 96-97 0.5657586528071832 1102 98-99 0.8125937964898498 1102 100-101 1.1849650260567657 1103 1 -0.01414295794894116 1103 2 -0.024289122271721197 1103 3 -0.00941898280802178 1103 4 0.058581146783154736 1103 5 0.0017125959560004844 1103 6 0.10644174688084718 1103 7 0.1565838751999138 1103 8 0.23283294836162582 1103 9 0.05463903482172583 1103 10-11 0.2679398070929011 1103 12-13 0.21793314601197267 1103 14-15 0.20837941517780223 1103 16-17 0.28313721025550365 1103 18-19 0.07019687919164141 1103 20-21 0.03309478120279863 1103 22-23 -0.20674688164100985 1103 24-25 -0.17300077361034738 1103 26-27 0.10986946856282742 1103 28-29 -0.0028236707319564402 1103 30-31 0.13810331421816358 1103 32-33 0.015870958043898042 1103 34-35 -0.02986254771108321 1103 36-37 0.181424903199634 1103 38-39 0.1403941228179093 1103 40-41 0.10678765523841349 1103 42-43 -0.025588366153840525 1103 44-45 0.2954415655421414 1103 46-47 0.19176122221232106 1103 48-49 0.3169670608034352 1103 50-51 0.47160524315446395 1103 52-53 0.16254178054175128 1103 54-55 0.2854864289160375 1103 56-57 0.25292323409572504 1103 58-59 0.2362366698791405 1103 60-61 0.20835286173186063 1103 62-63 0.22354380200543744 1103 64-65 0.1762545357680949 1103 66-67 0.3335518440916232 1103 68-69 0.5747216115090978 1103 70-71 0.5724704155794527 1103 72-73 0.5925771770997734 1103 74-75 0.6118650072570162 1103 76-77 0.6057970172275695 1103 78-79 0.5922664703741454 1103 80-81 0.5798112524199048 1103 82-83 0.687761925038668 1103 84-85 0.5921130181005125 1103 86-87 0.8081738130617957 1103 88-89 0.9595069738987085 1103 90-91 0.7152703478249158 1103 92-93 0.6602979707305181 1103 94-95 0.6968072891733144 1103 96-97 0.7345351550307839 1103 98-99 0.9023721258586228 1103 100-101 0.9062547147525954 1104 1 0.03095775274457324 1104 2 -0.18324357196649643 1104 3 0.06481120047769551 1104 4 0.08745240630096163 1104 5 0.04859801466368907 1104 6 -0.016823099231750405 1104 7 0.08079074336391301 1104 8 -0.0282113564452402 1104 9 0.1506760716899933 1104 10-11 0.13079828658243287 1104 12-13 0.2159327541356575 1104 14-15 0.17858770199480745 1104 16-17 0.23164572597080735 1104 18-19 0.13469850517456194 1104 20-21 0.032400643702821696 1104 22-23 0.16514146095001792 1104 24-25 0.18334010410145396 1104 26-27 -0.01897050160597047 1104 28-29 0.0352202285284946 1104 30-31 0.2332510348802188 1104 32-33 0.06780292345549555 1104 34-35 0.2791471140145987 1104 36-37 0.5348525298721327 1104 38-39 0.3796399279946243 1104 40-41 0.5068136284358999 1104 42-43 0.3925656837663354 1104 44-45 0.6304988508721365 1104 46-47 0.5820275006480387 1104 48-49 0.7359124578516045 1104 50-51 0.6585021447494626 1104 52-53 0.821450663223402 1104 54-55 0.943031339004726 1104 56-57 0.9487049438004433 1104 58-59 0.9178417223495643 1104 60-61 0.8222590027982122 1104 62-63 0.394249984385894 1104 64-65 0.4024393160819386 1104 66-67 0.4490938409292724 1104 68-69 0.713145843439122 1104 70-71 0.3561592475602069 1104 72-73 0.5282350356628065 1104 74-75 0.18446692927363273 1104 76-77 0.4015800939426768 1104 78-79 0.5819371879482809 1104 80-81 0.4018735850560624 1104 82-83 0.42351349975869823 1104 84-85 0.824963492984736 1104 86-87 0.685906125086472 1104 88-89 0.37529761473799184 1104 90-91 0.522609978519462 1104 92-93 0.8585251101397553 1104 94-95 0.8905969609541202 1104 96-97 0.7385830246969789 1104 98-99 1.2200205338231278 1104 100-101 1.4060861168040226 1105 1 -0.06371881825389636 1105 2 -0.014042579143850986 1105 3 0.03520349086935326 1105 4 0.05372510995056956 1105 5 -0.1242274976525124 1105 6 0.15793787961252548 1105 7 0.13934532620815077 1105 8 0.21354050957746296 1105 9 0.06794159798456434 1105 10-11 0.08306013907478871 1105 12-13 0.047759977906061124 1105 14-15 0.13181952787450513 1105 16-17 0.0904294734282658 1105 18-19 0.0965290443274398 1105 20-21 0.07745288337809342 1105 22-23 0.1346903449205925 1105 24-25 0.28500137953805194 1105 26-27 0.4081616477475336 1105 28-29 0.13940724298176832 1105 30-31 0.019178132307658302 1105 32-33 -0.16192508077386236 1105 34-35 0.1988913367366152 1105 36-37 0.2210414977129247 1105 38-39 0.278167942976566 1105 40-41 0.2609410556094858 1105 42-43 0.4543594848971537 1105 44-45 0.24752065175206184 1105 46-47 0.48162405444235645 1105 48-49 0.5913875432002982 1105 50-51 0.4372541272585835 1105 52-53 0.518845847854358 1105 54-55 0.4033016322816678 1105 56-57 0.23305051418063982 1105 58-59 0.3327103167169909 1105 60-61 0.34124964804333047 1105 62-63 0.36026395379476384 1105 64-65 0.3192489772856888 1105 66-67 0.1722146014959378 1105 68-69 0.2356646828760418 1105 70-71 0.21296048590036065 1105 72-73 0.36553264052297507 1105 74-75 0.6664753790187063 1105 76-77 0.748753282354965 1105 78-79 0.5330128951552062 1105 80-81 0.6180286822326124 1105 82-83 0.440569798537382 1105 84-85 0.5125133357280802 1105 86-87 0.5899986063366427 1105 88-89 0.49651516655204375 1105 90-91 0.40578866867456753 1105 92-93 0.5468959580712252 1105 94-95 0.5251309257035395 1105 96-97 0.7657433996040126 1105 98-99 0.9076958234710695 1105 100-101 1.6181966713689455 1106 1 -0.34964380201661527 1106 2 -0.21708977550670028 1106 3 -0.44897869142118196 1106 4 -0.2699515516644979 1106 5 -0.27676321967026496 1106 6 -0.24145863478452867 1106 7 -0.35322611782735436 1106 8 -0.4758673709161485 1106 9 -0.5549475555118661 1106 10-11 -0.9436975465714568 1106 12-13 -1.1342667053587192 1106 14-15 -0.8799930939050995 1106 16-17 -0.9436417541101605 1106 18-19 -0.7481748959193695 1106 20-21 -0.44325641517137626 1106 22-23 -0.5181768882467637 1106 24-25 -0.5131692201588507 1106 26-27 -0.6439058993405808 1106 28-29 -0.4502563746843933 1106 30-31 -0.4329692480777041 1106 32-33 -0.3247917726985179 1106 34-35 -0.17139985183233364 1106 36-37 -0.432157246604028 1106 38-39 -1.032694797876431 1106 40-41 -0.6863148439358682 1106 42-43 -0.7314491316865173 1106 44-45 -0.3754970468840071 1106 46-47 -0.23093548225389782 1106 48-49 -0.08724960769816192 1106 50-51 -0.36994169434782975 1106 52-53 -0.7679992484868379 1106 54-55 -0.8982382031805471 1106 56-57 -0.9320285072525749 1106 58-59 -1.0711920431011492 1106 60-61 -1.1169664160398085 1106 62-63 -1.1901663341463475 1106 64-65 -0.8926745992460354 1106 66-67 -0.9377929759017647 1106 68-69 -0.7037702597522291 1106 70-71 -0.5275068771652371 1106 72-73 -0.33376645018929807 1106 74-75 -0.4656149002625227 1106 76-77 -0.4509585059108936 1106 78-79 -0.6214374728898271 1106 80-81 -0.5868519737535323 1106 82-83 -0.7381011800294068 1106 84-85 -0.6908721373180349 1106 86-87 -0.8303507658218336 1106 88-89 -0.782116571919488 1106 90-91 -0.9472436435142164 1106 92-93 -0.914053381612689 1106 94-95 -0.6582894574962879 1106 96-97 -0.5135851888350444 1106 98-99 -0.3443811953641749 1106 100-101 -0.04305305800970771 1107 1 0.08419387185139371 1107 2 0.05995224876083327 1107 3 0.18876546365428126 1107 4 0.22237236073561917 1107 5 0.1421661973170103 1107 6 0.10252257267329412 1107 7 0.2019492299086636 1107 8 0.27749900966982466 1107 9 0.28875620299657356 1107 10-11 -0.0011942171840644278 1107 12-13 0.08226370304613084 1107 14-15 0.011006462176581522 1107 16-17 0.34997322073446924 1107 18-19 0.156031230153431 1107 20-21 0.04136366473392172 1107 22-23 0.04910448198112505 1107 24-25 0.14571654486038454 1107 26-27 0.35742585561650486 1107 28-29 0.2952397040371224 1107 30-31 0.5796731757163229 1107 32-33 0.40194875704401056 1107 34-35 0.388196182497353 1107 36-37 0.6114577282405946 1107 38-39 0.38000126604781315 1107 40-41 0.3129082990058336 1107 42-43 0.5262454525099827 1107 44-45 0.4871379782713703 1107 46-47 0.05825880599707034 1107 48-49 0.31279478413374306 1107 50-51 0.27076191618783696 1107 52-53 0.3776984431379091 1107 54-55 0.12852637213759266 1107 56-57 0.20780576324165878 1107 58-59 0.5849192324241557 1107 60-61 0.568221509381921 1107 62-63 0.521453227854245 1107 64-65 0.7575917517453874 1107 66-67 0.8515677817188347 1107 68-69 0.9057665854682497 1107 70-71 1.2319171388158239 1107 72-73 0.9502487910063202 1107 74-75 0.8854505037031473 1107 76-77 0.6623054035297713 1107 78-79 0.8156463536860841 1107 80-81 0.8152917817954481 1107 82-83 1.1148971182837037 1107 84-85 0.9551584930459818 1107 86-87 1.0008030518757103 1107 88-89 0.5152717086368916 1107 90-91 0.6856821481401205 1107 92-93 0.8773789948300461 1107 94-95 1.4772813474755964 1107 96-97 1.473827877160815 1107 98-99 1.501162974508567 1107 100-101 1.7370778978944053 1108 1 0.03282353158893869 1108 2 0.18724873000575215 1108 3 0.029405342878529694 1108 4 0.32359173130814867 1108 5 0.08640031646693558 1108 6 0.3823623125409057 1108 7 0.22832247343438894 1108 8 0.4007251158131311 1108 9 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72-73 -1.2168388111783806 2316 74-75 -1.3098159797152675 2316 76-77 -1.3451509560175836 2316 78-79 -1.317586431092412 2316 80-81 -1.7887982835313672 2316 82-83 -1.5455373228686184 2316 84-85 -1.6171382977297348 2316 86-87 -1.8466249279907778 2316 88-89 -1.6372690759988124 2316 90-91 -1.8365173663710017 2316 92-93 -1.700861464956482 2316 94-95 -1.5426915222634534 2316 96-97 -1.6954015877304869 2316 98-99 -1.8621772603823743 2316 100-101 -2.237880157294253 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1673.0 3 301.0 4 243.0 5 280.0 6 338.0 7 421.0 8 462.0 9 572.0 10 714.0 11 1060.0 12 1517.0 13 2153.0 14 2440.0 15 3109.0 16 4155.0 17 6069.0 18 4978.0 19 2957.0 20 2929.0 21 3078.0 22 3205.0 23 3484.0 24 3841.0 25 4361.0 26 5083.0 27 5770.0 28 6676.0 29 7988.0 30 9808.0 31 12216.0 32 15696.0 33 20702.0 34 28954.0 35 41571.0 36 63011.0 37 94100.0 38 83646.0 39 12603.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.83927128671584 19.266638866446005 15.505790264710226 22.38829958212793 2 14.688050153364578 20.36807498692114 40.981663544382386 23.9622113153319 3 18.042643525221237 25.405521561613448 31.29565034306267 25.256184570102647 4 11.878950750208391 16.86992046401778 38.43493852459016 32.816190261183664 5 12.677328250737533 37.42719637387471 35.189956952787 14.705518422600763 6 30.039561698029722 36.862219445962715 18.15586961991272 14.942349236094843 7 26.66520648235093 33.663898822238465 21.782841677708404 17.888053017702205 8 25.682683236279647 33.67795525770737 21.024539943828145 19.614821562184847 9 26.007611983995314 16.26275441050497 21.16023117877319 36.56940242672652 10-11 23.937615912714335 26.610956259557717 28.235740702580177 21.215687125147774 12-13 25.366053089898 24.375524598377243 28.623714123977646 21.63470818774712 14-15 22.18969142368871 25.374998780095055 27.899930926271114 24.53537886994512 16-17 20.8220559531555 28.92094990240729 28.144762524398175 22.11223162003904 18-19 20.567055973681388 27.015398427952903 31.37881043592916 21.038735162436552 20-21 21.53334569342844 25.906976189469415 31.371764555367264 21.18791356173488 22-23 21.089182554690076 25.899393681800042 31.469779504883505 21.541644258626373 24-25 21.13131247425139 26.010104295409704 31.414818187731736 21.44376504260717 26-27 20.727308602571373 26.33200598013632 31.334244736753664 21.606440680538647 28-29 20.441071697736735 26.619588944687656 31.56296663388143 21.376372723694185 30-31 20.74472526936237 26.22404659006491 31.28323310757685 21.747995032995874 32-33 20.523756541805742 26.449607762762078 31.11795746009851 21.908678235333667 34-35 21.152836704061357 26.452840174566404 30.838040205800947 21.55628291557129 36-37 21.077887679285645 26.124936438909252 31.024859569291618 21.772316312513485 38-39 20.877974738439857 26.53103485661625 30.921884317233157 21.669106087710738 40-41 20.892591909971053 26.53588039506922 31.032774266292268 21.538753428667455 42-43 20.811766109008904 26.655029220110375 30.471967126020534 22.061237544860187 44-45 20.788931537744237 26.805564591329993 30.292325374623207 22.11317849630256 46-47 21.086931298802554 27.006617791373124 29.72443399612453 22.182016913699794 48-49 21.13542876539555 27.179343694454296 29.42107209513925 22.264155445010907 50-51 21.06099961499043 27.352056005335907 29.43750643942064 22.149437940253023 52-53 21.43442138365462 27.494323993912328 28.8984371864366 22.172817435996457 54-55 20.85667623156155 27.13708164429436 29.407822392264798 22.598419731879297 56-57 21.127664510701408 27.474029761427232 29.043085335894762 22.355220391976598 58-59 21.37602872571904 27.116402259808602 28.982880139291982 22.524688875180377 60-61 21.285439234018195 26.883624016721157 29.75063528268996 22.08030146657069 62-63 21.10469024437843 27.022573020794933 29.73187761562981 22.140859119196826 64-65 20.886436863353904 27.174624788055663 29.62060337463086 22.318334973959576 66-67 20.794559693337728 27.126949161361942 29.29715279343659 22.781338351863738 68-69 20.78745961884526 27.06914120937493 29.676083515816405 22.4673156559634 70-71 21.023666695216896 27.101721578878323 29.197669867545002 22.67694185835978 72-73 20.934652391923024 26.83897547093102 29.349993224014092 22.876378913131862 74-75 20.614434527681645 27.2484018977735 29.193229424162787 22.94393415038207 76-77 20.816726588284276 26.71947575451364 29.352195993564028 23.11160166363806 78-79 20.825319244438564 26.747794183018296 29.35368315599338 23.073203416549752 80-81 20.816636308439588 27.078129065833984 29.21448087431694 22.89075375140949 82-83 20.741784750154494 26.839337792859695 29.418996714984225 22.999880742001583 84-85 20.723829346688998 26.670460889691093 29.0326357383287 23.573074025291206 86-87 20.669039315653738 26.78585560085728 29.050026180082106 23.49507890340688 88-89 20.620989676550714 26.24007683777019 29.11455527213558 24.024378213543525 90-91 20.323005904175854 26.16397693837151 29.76762742330317 23.745389734149466 92-93 20.62377364626809 26.115561819068784 29.60052035340669 23.660144181256438 94-95 20.143422407718575 26.27286031763239 29.339693208303974 24.24402406634506 96-97 19.878392895664835 26.549222258718085 29.479045051432067 24.093339794185017 98-99 20.290427010535947 26.727568015299784 28.748132520062537 24.233872454101736 100-101 20.120326507604418 26.471940075231164 28.846708365402336 24.56102505176208 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 733.0 1 425.5 2 124.5 3 131.5 4 124.0 5 133.5 6 222.5 7 450.0 8 745.0 9 912.5 10 874.0 11 811.5 12 832.0 13 899.0 14 970.5 15 1008.5 16 1029.5 17 1056.0 18 1130.5 19 1217.5 20 1312.0 21 1463.0 22 1679.5 23 1933.0 24 2233.5 25 2655.5 26 3228.0 27 3756.5 28 4381.0 29 5168.0 30 5937.0 31 6816.0 32 7839.0 33 8775.5 34 9739.5 35 10420.5 36 11069.5 37 12074.0 38 12888.5 39 13224.0 40 13451.0 41 14295.5 42 15204.5 43 16006.5 44 16601.5 45 16991.5 46 17329.5 47 18291.5 48 20759.0 49 20517.0 50 17803.5 51 16111.0 52 14966.0 53 14573.5 54 13829.5 55 12364.5 56 10801.5 57 9418.0 58 8410.0 59 7335.5 60 6254.5 61 5144.5 62 4015.5 63 3038.0 64 2262.5 65 1735.0 66 1233.5 67 906.5 68 664.5 69 436.0 70 346.5 71 265.5 72 180.5 73 142.5 74 113.5 75 91.0 76 66.0 77 35.5 78 21.0 79 17.5 80 12.5 81 8.5 82 6.0 83 4.0 84 5.0 85 4.5 86 3.0 87 4.0 88 3.0 89 2.0 90 1.0 91 2.5 92 5.0 93 3.5 94 2.5 95 2.0 96 1.0 97 1.0 98 1.5 99 1.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3756242372837348 2 0.3239109926346492 3 0.3161215499260003 4 0.3228291255917813 5 0.32607472672038496 6 0.24060723033382092 7 0.22394647787365524 8 0.23346690785089275 9 0.22697570559368535 10-11 0.24861304645104337 12-13 0.23736162920521717 14-15 0.22989674660942866 16-17 0.23022130672228905 18-19 0.22621839866367785 20-21 0.2169143420950139 22-23 0.22070087674505154 24-25 0.21074769995066683 26-27 0.20999039302065933 28-29 0.2342242147809003 30-31 0.24266277771526992 32-33 0.23606338875377572 34-35 0.24580019213958682 36-37 0.21496698141785167 38-39 0.2139933010792706 40-41 0.21182956699353478 42-43 0.21832076925074215 44-45 0.22373010446508165 46-47 0.22903125297513435 48-49 0.24093179044668125 50-51 0.24590837884387362 52-53 0.26668022606693725 54-55 0.23865986965665867 56-57 0.20836759245635747 58-59 0.21085588665495364 60-61 0.20598748496204808 62-63 0.21507516812213848 64-65 0.20728572541348958 66-67 0.20339100405916516 68-69 0.20209276360772366 70-71 0.21388511437498378 72-73 0.21128863347210083 74-75 0.21464242130499128 76-77 0.2176716490250214 78-79 0.2157242883478592 80-81 0.21723890220787428 82-83 0.21182956699353478 84-85 0.20263369712915763 86-87 0.20241732372058402 88-89 0.20176820349486327 90-91 0.20880033927350464 92-93 0.20317463065059158 94-95 0.20317463065059158 96-97 0.20003721622627466 98-99 0.21226231381068192 100-101 0.19884716247911996 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 462164.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 63.9977858677295 #Duplication Level Percentage of deduplicated Percentage of total 1 81.51522694543297 52.16794039013193 2 10.966298765509958 14.036376803133052 3 3.2793294517545375 6.296094721293769 4 1.4351146612215049 3.6737664313797205 5 0.7568195554585853 2.4217387925374383 6 0.45231559687150014 1.7368318027929917 7 0.2957561246055815 1.324941599210427 8 0.2056569650198475 1.0529272327637869 9 0.17670272350133887 1.0177724754794968 >10 0.8461743584636557 9.673223428796094 >50 0.047295116371547354 2.0876152860314305 >100 0.020607157726457322 2.3064084268044294 >500 0.0016891112890538784 0.7259954534316904 >1k 0.001013466773432327 1.4783671562137874 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2997 0.6484711054950191 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2250 0.48684016929055485 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1591 0.3442500930405657 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 815 0.17634432798746766 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 725 0.15687072121584544 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 698 0.1510286391843588 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 607 0.13133865900416303 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 513 0.11099955859824652 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 2.1637340857357995E-4 0.0 6 0.0 0.0 0.0 2.1637340857357995E-4 0.0 7 0.0 0.0 0.0 2.1637340857357995E-4 0.0 8 0.0 0.0 0.0 2.1637340857357995E-4 0.0 9 0.0 0.0 0.0 2.1637340857357995E-4 0.0 10-11 0.0 0.0 0.0 2.1637340857357995E-4 0.0 12-13 0.0 0.0 0.0 2.1637340857357995E-4 0.0 14-15 0.0 0.0 0.0 2.1637340857357995E-4 0.0 16-17 0.0 0.0 0.0 2.1637340857357995E-4 0.0 18-19 0.0 0.0 0.0 2.1637340857357995E-4 0.0 20-21 0.0 0.0 0.0 3.245601128603699E-4 0.0 22-23 0.0 0.0 0.0 4.327468171471599E-4 0.0 24-25 0.0 0.0 0.0 8.654936342943198E-4 0.0 26-27 0.0 0.0 0.0 0.0010818670428678998 0.0 28-29 0.0 0.0 0.0 0.0027046676071697494 0.0 30-31 0.0 0.0 0.0 0.005733895327199869 0.0 32-33 0.0 0.0 0.0 0.009195869864377149 0.0 34-35 0.0 0.0 0.0 0.012225097584407266 0.0 36-37 0.0 0.0 0.0 0.015146138600150596 0.0 38-39 0.0 0.0 0.0 0.019257233363048614 0.0 40-41 0.0 0.0 0.0 0.024017448351667373 0.0 42-43 0.0 0.0 0.0 0.03570161241464069 0.0 44-45 0.0 0.0 0.0 0.054309725551968564 0.0 46-47 0.0 0.0 0.0 0.07508157277503225 0.0 48-49 0.0 0.0 0.0 0.12235916254835946 0.0 50-51 0.0 0.0 0.0 0.17190867311170926 0.0 52-53 0.0 0.0 0.0 0.24244640430669634 0.0 54-55 0.0 0.0 0.0 0.3751914904665876 0.0 56-57 0.0 0.0 0.0 0.4906267039405925 0.0 58-59 0.0 0.0 0.0 0.6177460814775707 0.0 60-61 0.0 0.0 0.0 0.785543659826382 0.0 62-63 0.0 0.0 0.0 1.0065690966842937 0.0 64-65 0.0 0.0 0.0 1.155974935304351 0.0 66-67 0.0 0.0 0.0 1.43607031270285 0.0 68-69 0.0 0.0 0.0 1.8142910308894677 0.0 70-71 0.0 0.0 0.0 2.074263681290624 0.0 72-73 0.0 0.0 0.0 2.50041110947629 0.0 74-75 0.0 0.0 0.0 3.064172025514752 0.0 76-77 0.0 0.0 0.0 3.4621909105858526 0.0 78-79 0.0 0.0 0.0 3.8853091110514884 0.0 80-81 0.0 0.0 0.0 4.492344708804667 0.0 82-83 0.0 0.0 0.0 5.180844894885798 0.0 84-85 0.0 0.0 0.0 5.8814619918470505 0.0 86-87 0.0 0.0 0.0 6.908586562345834 0.0 88-89 0.0 0.0 0.0 8.201525865277262 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCGCT 40 5.380373E-4 47.50792 7 TACGGGA 45 9.585526E-4 42.25219 2 CACGGCG 50 0.0016134884 38.006336 7 GTATCAA 2980 0.0 32.710026 1 GGTATCA 2020 0.0 29.424013 1 GTATTAC 65 0.0058110063 29.26105 1 GGCTCGT 65 0.005838825 29.232473 6 GGGCGTA 70 0.008379626 27.147383 9 CGGGTAG 35 0.008397225 27.138548 60-61 TATCAAC 3775 0.0 25.813013 2 ATCAACG 3780 0.0 25.653116 3 TCAACGC 3780 0.0 25.653116 4 CAACGCA 3835 0.0 25.287956 5 GTACATG 2115 0.0 24.730083 1 AACGCAG 3980 0.0 24.348152 6 TACATGG 2195 0.0 23.604445 2 GCACCTA 105 0.0021934966 22.620365 6 ACATGGG 2210 0.0 22.58389 3 GAGTACT 2185 0.0 22.39504 12-13 ATACGTC 170 3.119958E-6 22.371237 5 >>END_MODULE