FastQCFastQC Report
Thu 26 May 2016
SRR937370_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937370_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences462164
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32040.6932604010697502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26890.5818280956543564No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18900.40894574220406604No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15720.3401389982776677No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5910.12787668446698575No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT4920.10645571701820133No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTGGTCG704.2994725E-640.7213339
ATCGCGA250.001615995837.99835652-53
TCGCGAA300.00395139231.66529754-55
GTGTACG908.924745E-426.3963151
GTGTTAC1309.982345E-625.584121
GTAAGGT1501.0572185E-625.3349762
GAGTACT26150.024.43539612-13
GTACATG22350.024.2350061
GTATCAA46950.023.781981
ATACGTC1401.7756034E-523.7515415
GGTATCA36000.023.492721
ACATGGG21800.023.3157353
GTACTTT28000.022.98561314-15
TCTAAGG1452.3292687E-522.9325243
TACATGG22900.022.6106382
ATAACCC1703.1404634E-622.3543933
AGTACTT27100.022.26400212-13
CACTAGC1503.0215799E-522.1729031
CATGGGG9000.021.1124824
CATGGGT2509.444193E-920.9013584
Produced by FastQC (version 0.11.5)