FastQCFastQC Report
Thu 26 May 2016
SRR937369_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937369_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547799
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35140.6414761618768928No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25660.4684199861628079No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17990.32840512669793115No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT9490.17323872442264407No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT8770.16009521740638447No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN8530.1557140484009646No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT8220.1500550384356306No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6070.1108070660954109No Hit
CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC6020.1098943225526151No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA43650.029.4155831
ACACCGT650.00584227429.2298956
GTACATG26950.028.7625081
TACACCG700.008351590527.1669245
GGTATCA30650.026.9970741
TACATGG27800.026.8493252
ACATGGG27150.026.7916893
TACACTG4550.025.077165
CATGGGG12350.024.2544774
ATCAACG53050.023.927893
TATCAAC54050.023.661112
TCAACGC53700.023.5518844
GAGTACT28650.023.37626512-13
CAACGCA54450.023.225355
AACGCAG54900.023.0138846
AGTACTT29200.022.93595712-13
TAGCACT2303.5761332E-922.7396184
GTACACT3600.022.4525014
CATGGGA16350.022.3919184
TAGATTG1503.0087864E-522.1863215
Produced by FastQC (version 0.11.5)