##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937369_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547799 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79225409319842 33.0 31.0 34.0 30.0 34.0 2 31.895215215799954 33.0 31.0 34.0 30.0 34.0 3 31.931204693692393 33.0 31.0 34.0 30.0 34.0 4 35.55902621216906 37.0 35.0 37.0 33.0 37.0 5 35.542751994801016 37.0 35.0 37.0 33.0 37.0 6 35.61263529141163 37.0 35.0 37.0 33.0 37.0 7 35.56431282276893 37.0 35.0 37.0 33.0 37.0 8 35.558462136659614 37.0 35.0 37.0 33.0 37.0 9 37.19948922871345 39.0 37.0 39.0 34.0 39.0 10-11 37.108297934096264 39.0 37.0 39.0 33.5 39.0 12-13 37.00014968994102 39.0 37.0 39.0 33.0 39.0 14-15 38.17119782986095 40.0 38.0 41.0 33.0 41.0 16-17 38.13219356004666 40.0 38.0 41.0 33.0 41.0 18-19 38.123137318615036 40.0 38.0 41.0 33.5 41.0 20-21 38.06835353843289 40.0 38.0 41.0 33.0 41.0 22-23 37.94240314421896 40.0 38.0 41.0 33.0 41.0 24-25 37.79769404471348 40.0 37.5 41.0 33.0 41.0 26-27 37.62434031460444 40.0 37.0 41.0 32.5 41.0 28-29 37.44333414263261 39.5 37.0 41.0 32.0 41.0 30-31 37.387963468352446 39.0 37.0 41.0 32.0 41.0 32-33 37.206149518345235 39.0 36.0 41.0 32.0 41.0 34-35 36.993935731901665 39.0 36.0 41.0 31.0 41.0 36-37 37.00416028506807 39.0 36.0 41.0 31.0 41.0 38-39 36.965543018515916 40.0 36.0 41.0 31.0 41.0 40-41 36.7997440667106 39.5 36.0 41.0 31.0 41.0 42-43 36.54220891239305 39.0 36.0 41.0 30.0 41.0 44-45 36.26190445765691 39.0 35.0 41.0 30.0 41.0 46-47 35.860794744057586 39.0 35.0 41.0 28.5 41.0 48-49 35.49115368958323 39.0 35.0 41.0 27.0 41.0 50-51 34.52816726573067 38.0 34.0 40.0 25.0 40.5 52-53 34.6372191259933 38.0 34.5 40.0 25.5 40.5 54-55 34.98430993849934 39.0 35.0 40.0 26.0 41.0 56-57 34.78011004036152 38.5 34.5 40.0 24.5 41.0 58-59 34.539216026316225 38.0 34.0 40.0 23.5 41.0 60-61 34.2465694533944 38.0 34.0 40.0 22.5 41.0 62-63 33.94044257108903 38.0 34.0 40.0 22.0 41.0 64-65 33.59323218917888 37.0 33.5 40.0 21.5 41.0 66-67 33.20773586662261 36.5 33.0 39.5 19.5 41.0 68-69 32.74388598737858 36.0 32.5 39.0 18.0 41.0 70-71 32.29452956285061 35.5 32.0 39.0 14.5 40.0 72-73 31.877604741885257 35.0 32.0 38.0 10.0 40.0 74-75 31.444122753053584 35.0 31.0 37.0 7.5 39.5 76-77 31.00728095524088 35.0 31.0 37.0 6.0 39.0 78-79 30.545255650338902 35.0 31.0 36.0 2.0 39.0 80-81 30.10793101119206 34.5 30.0 36.0 2.0 37.5 82-83 29.670609110275848 34.0 30.0 35.5 2.0 37.0 84-85 29.235117260162944 34.0 29.0 35.0 2.0 37.0 86-87 28.843668024220563 34.0 29.0 35.0 2.0 36.0 88-89 28.482934433980347 34.0 29.0 35.0 2.0 36.0 90-91 28.078834572534817 34.0 28.5 35.0 2.0 35.5 92-93 27.681864150902065 34.0 27.0 35.0 2.0 35.0 94-95 27.197683821985798 33.5 26.5 35.0 2.0 35.0 96-97 26.62405462587555 33.0 25.0 35.0 2.0 35.0 98-99 25.973740368273766 33.0 24.5 35.0 2.0 35.0 100-101 24.227397275277976 31.0 11.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.1755983252831257 1101 2 0.013719696508871948 1101 3 0.19979700731270356 1101 4 0.14925283024764724 1101 5 0.10904028656736386 1101 6 -0.08172415694848922 1101 7 -0.054177195681731405 1101 8 -0.01215896328240973 1101 9 -0.06865193637509037 1101 10-11 -0.0017309638249614068 1101 12-13 0.09051544532542977 1101 14-15 0.2167560842765468 1101 16-17 0.09316530368372611 1101 18-19 0.1426642003519305 1101 20-21 0.0781913069639728 1101 22-23 0.02264415593436553 1101 24-25 0.11863686162363507 1101 26-27 0.13257877023016107 1101 28-29 0.18465441806932148 1101 30-31 0.19259691199749085 1101 32-33 0.17698343853616905 1101 34-35 0.02553127525165877 1101 36-37 0.07032427914299433 1101 38-39 0.2730400975304974 1101 40-41 0.33433560983581856 1101 42-43 0.5136815132272119 1101 44-45 0.3035056190448344 1101 46-47 0.4937205521921868 1101 48-49 0.6376734465239267 1101 50-51 0.5123015080304469 1101 52-53 0.6788770273915787 1101 54-55 0.7893909404379897 1101 56-57 0.7769848071862313 1101 58-59 0.7202435243190948 1101 60-61 0.8375722123716898 1101 62-63 0.8412053484416688 1101 64-65 0.7656917273508768 1101 66-67 0.8744593545598107 1101 68-69 0.7516653896873038 1101 70-71 0.8562342992826757 1101 72-73 0.9873822307370155 1101 74-75 1.041625836078083 1101 76-77 0.9583770363538235 1101 78-79 1.0430843556185714 1101 80-81 1.0020920008281315 1101 82-83 0.8850677045683142 1101 84-85 0.9233446383546884 1101 86-87 1.1067568745858338 1101 88-89 0.9710895565064774 1101 90-91 1.1922267563075515 1101 92-93 0.8720183574928733 1101 94-95 0.8101566077731057 1101 96-97 0.781350106717742 1101 98-99 0.8278342851141858 1101 100-101 1.2608661298256436 1102 1 0.01643842584453381 1102 2 -0.013030825696194626 1102 3 -0.01839994724788596 1102 4 -0.08080801799501813 1102 5 -0.013317658517856046 1102 6 -0.06881433949321547 1102 7 -0.025498298821013066 1102 8 0.012095570063038963 1102 9 0.2634038865898205 1102 10-11 0.0333763531005431 1102 12-13 0.04862400343399287 1102 14-15 0.2453524878729496 1102 16-17 0.26993171908650737 1102 18-19 0.2293267801963239 1102 20-21 0.1433375243829218 1102 22-23 0.3947765689758711 1102 24-25 0.2018301834533247 1102 26-27 0.4011511069843152 1102 28-29 0.3104990280048483 1102 30-31 0.34080173747504716 1102 32-33 0.4127522152140344 1102 34-35 0.2997661313047004 1102 36-37 0.4296778044056069 1102 38-39 0.6023229359042475 1102 40-41 0.39765486633689306 1102 42-43 0.6176896263262321 1102 44-45 0.4729679748708193 1102 46-47 0.8328745799825796 1102 48-49 1.0352796282210193 1102 50-51 1.0449302429319047 1102 52-53 0.9999551916515586 1102 54-55 0.9928745780125325 1102 56-57 1.0235745810487273 1102 58-59 0.9590516858545257 1102 60-61 1.194444128334517 1102 62-63 1.3783704560613188 1102 64-65 1.2062314906179168 1102 66-67 1.2574960148691972 1102 68-69 1.1319699336282127 1102 70-71 1.09811968662261 1102 72-73 1.1523248638614625 1102 74-75 0.9221566688816409 1102 76-77 1.108171938421453 1102 78-79 1.2571316719386125 1102 80-81 1.2892987334894137 1102 82-83 1.2247870761058657 1102 84-85 0.9038603347794769 1102 86-87 1.0297475203037543 1102 88-89 1.00458484909268 1102 90-91 0.7849628081345124 1102 92-93 0.5307535616826193 1102 94-95 0.7118586935661035 1102 96-97 0.9638925642602025 1102 98-99 1.042266822273998 1102 100-101 1.3588476937162994 1103 1 0.005047377868120151 1103 2 -0.03891754060730079 1103 3 0.024886035062486656 1103 4 0.1920634195105677 1103 5 -0.04774765941127157 1103 6 -0.04799775770102599 1103 7 0.11420955430147472 1103 8 0.20185702811717476 1103 9 0.22730989927632095 1103 10-11 0.11544215315414164 1103 12-13 0.1333923854584711 1103 14-15 0.1207656925544498 1103 16-17 0.1388844127343205 1103 18-19 0.10511968647703895 1103 20-21 -0.06417498343446226 1103 22-23 0.1362612022288019 1103 24-25 0.2373881214502802 1103 26-27 0.09921016175068331 1103 28-29 -0.17315146784077484 1103 30-31 -0.16785880929526087 1103 32-33 -0.24261274502897834 1103 34-35 -0.06680225965393305 1103 36-37 0.09797495441007698 1103 38-39 -0.034470149059572464 1103 40-41 0.1467726185034195 1103 42-43 -0.009275887834405694 1103 44-45 0.1652421640962345 1103 46-47 -0.09903040527606777 1103 48-49 0.016221958248713975 1103 50-51 -0.1822820772682192 1103 52-53 0.13750767981380818 1103 54-55 -0.016243844215637182 1103 56-57 0.2298698102098129 1103 58-59 0.32202407931472266 1103 60-61 0.2458601919457024 1103 62-63 0.19786143256448696 1103 64-65 0.22694197931620863 1103 66-67 0.0941126502663181 1103 68-69 0.1861779207630292 1103 70-71 0.09225106337594013 1103 72-73 0.2890006880372873 1103 74-75 0.2593205212893963 1103 76-77 0.14611976295464402 1103 78-79 0.4126083091225681 1103 80-81 0.19452968140328508 1103 82-83 0.2590774808230982 1103 84-85 0.28481829957890525 1103 86-87 0.44212020859107426 1103 88-89 0.4389858139814464 1103 90-91 0.180974824573358 1103 92-93 0.12114487768369386 1103 94-95 0.26071177138259216 1103 96-97 0.3983493676194456 1103 98-99 0.579042038927259 1103 100-101 0.9398748982191165 1104 1 0.00295478305054786 1104 2 0.07554439218936793 1104 3 0.07626975087008603 1104 4 -0.013831890674062208 1104 5 -0.036558785207255085 1104 6 0.06153806672126194 1104 7 -0.026224584030060782 1104 8 0.12470135508261393 1104 9 0.054675833034181665 1104 10-11 0.06891696191700447 1104 12-13 0.028524849714138156 1104 14-15 0.20343320026400846 1104 16-17 0.19291390931007157 1104 18-19 -0.056655915269132606 1104 20-21 0.015921836622403873 1104 22-23 0.05421196116988369 1104 24-25 0.0957925429329265 1104 26-27 0.1927861960331967 1104 28-29 -0.14312924027859708 1104 30-31 -0.300460735683977 1104 32-33 0.05494622809770533 1104 34-35 0.08150163717813541 1104 36-37 -0.04880204907424712 1104 38-39 0.015531853423503605 1104 40-41 -0.14536963789451107 1104 42-43 0.0024786878394351675 1104 44-45 0.23878836138086257 1104 46-47 0.28593445114589855 1104 48-49 0.34436135109584853 1104 50-51 0.46009697275381 1104 52-53 0.6530119050530985 1104 54-55 0.45501301428890173 1104 56-57 0.47137677885092444 1104 58-59 0.5171860801958204 1104 60-61 0.5118149758777832 1104 62-63 0.6007084628820891 1104 64-65 0.443142568046234 1104 66-67 0.6033340848451942 1104 68-69 0.46039294739605197 1104 70-71 0.589830561954173 1104 72-73 0.5976008522408911 1104 74-75 0.705834198248823 1104 76-77 0.7054783676244334 1104 78-79 0.5455932104001526 1104 80-81 0.6786349719635787 1104 82-83 0.8306036733017734 1104 84-85 0.8068381041365562 1104 86-87 1.0232583198490381 1104 88-89 1.0350098838280601 1104 90-91 1.1075913290388932 1104 92-93 0.8022894970116603 1104 94-95 0.6757338988206172 1104 96-97 0.6466322383791869 1104 98-99 0.7463845436330985 1104 100-101 0.6546036618860605 1105 1 -0.21437242996632122 1105 2 -0.16816735583272546 1105 3 -0.1318489981969364 1105 4 -0.04024441493141495 1105 5 -0.05652445172464837 1105 6 -0.14126272444160293 1105 7 -0.1563466171693335 1105 8 -0.2263118558443935 1105 9 -0.37154449835855985 1105 10-11 -0.07512972415553776 1105 12-13 -0.05115122134123595 1105 14-15 -0.278509067238609 1105 16-17 -0.2788216109168289 1105 18-19 -0.4358309787114365 1105 20-21 -0.3265779767825805 1105 22-23 -0.29994537574606994 1105 24-25 -0.20789550752236607 1105 26-27 -0.1127724694392569 1105 28-29 -0.22230150479568778 1105 30-31 -0.3138769172036078 1105 32-33 -0.430643971932156 1105 34-35 -0.3440796062910394 1105 36-37 -0.18976525253744825 1105 38-39 -0.18732491624361103 1105 40-41 -0.09188684195481756 1105 42-43 0.012596802483407998 1105 44-45 -0.03359837544552846 1105 46-47 -0.23451636240836393 1105 48-49 -0.2842445975532044 1105 50-51 -0.37239201814035283 1105 52-53 -0.40208027288390014 1105 54-55 -0.1442695278815691 1105 56-57 -0.024564779590633634 1105 58-59 -0.02479779799495674 1105 60-61 0.038381441021833496 1105 62-63 -0.07652881133794409 1105 64-65 -0.16154656466013506 1105 66-67 0.1855991617223438 1105 68-69 0.14596635387463408 1105 70-71 -0.09483319383026867 1105 72-73 0.07593250621910386 1105 74-75 -0.21806769009271676 1105 76-77 -0.053021035271520134 1105 78-79 -0.029487958431015926 1105 80-81 0.01238814407882316 1105 82-83 0.008638641207429743 1105 84-85 0.2316263187954597 1105 86-87 0.16967009772632835 1105 88-89 0.0599669669747982 1105 90-91 0.31405871223041615 1105 92-93 0.44424631892537647 1105 94-95 0.23160977581718356 1105 96-97 0.44669101580865345 1105 98-99 0.296429326436499 1105 100-101 0.6799432648118717 1106 1 -0.0536535389282804 1106 2 0.08716791173658223 1106 3 -0.14020650947998092 1106 4 -0.17587465348718467 1106 5 0.04025182164737373 1106 6 0.008938758472652353 1106 7 0.26224622615755067 1106 8 -0.07145208795270719 1106 9 0.13725627533582951 1106 10-11 -0.13614278024838455 1106 12-13 -0.25726018330126266 1106 14-15 -0.2858551342921203 1106 16-17 -0.12662544715151824 1106 18-19 0.02117659704614283 1106 20-21 -0.2032664036039833 1106 22-23 0.04692760942478458 1106 24-25 -0.19651172286731367 1106 26-27 -0.31134597040637857 1106 28-29 -0.09301394838047372 1106 30-31 0.11752218015293892 1106 32-33 0.03298836204592703 1106 34-35 0.050210367919149235 1106 36-37 -0.11187195788883031 1106 38-39 -0.23112730180131535 1106 40-41 -0.1369585698722915 1106 42-43 -0.3894220563173647 1106 44-45 -0.37602664752805737 1106 46-47 -0.24357503423299676 1106 48-49 0.014550534619587552 1106 50-51 -0.02679643464583137 1106 52-53 0.33678981093299143 1106 54-55 0.3879314714736779 1106 56-57 0.33353483356217595 1106 58-59 -0.07876298174738139 1106 60-61 0.06058082851377122 1106 62-63 0.188453458165597 1106 64-65 0.27819392534630794 1106 66-67 0.3873923596591524 1106 68-69 0.5321647742485851 1106 70-71 0.7088982697416171 1106 72-73 0.7655077802358683 1106 74-75 0.5846362106165017 1106 76-77 0.5202462116659419 1106 78-79 0.5829032407952859 1106 80-81 0.4577540370510995 1106 82-83 0.4035572421100717 1106 84-85 0.23495884619713436 1106 86-87 0.0019476605889998666 1106 88-89 0.2701136465363696 1106 90-91 0.23467444401635973 1106 92-93 0.33319296624968686 1106 94-95 0.007730020820407191 1106 96-97 0.42786848195952487 1106 98-99 0.2295047616395962 1106 100-101 0.2953043221942053 1107 1 -0.202103514395521 1107 2 -0.3622544532217091 1107 3 -0.3058651026375685 1107 4 -0.01700494475798564 1107 5 -0.15839218566785007 1107 6 -0.11714179819803405 1107 7 -0.08088683062464241 1107 8 -0.13508115258538567 1107 9 -0.3472925448613253 1107 10-11 -0.21826508722014637 1107 12-13 -0.048220975486465534 1107 14-15 0.017356427329715984 1107 16-17 -0.21899575804852134 1107 18-19 -0.085112711129959 1107 20-21 0.12065973047871381 1107 22-23 -0.07839020136071184 1107 24-25 -0.23211768209831973 1107 26-27 -0.1322478839618455 1107 28-29 -0.05675452556946681 1107 30-31 -0.43956778817749864 1107 32-33 -0.294691284682294 1107 34-35 -0.4372578510023075 1107 36-37 -0.5601078292479471 1107 38-39 -0.49777612645896596 1107 40-41 -0.4785661348841046 1107 42-43 -0.43480766673709326 1107 44-45 -0.3291779923694875 1107 46-47 -0.2712992859837371 1107 48-49 -0.4753954933031608 1107 50-51 -0.3768767236863795 1107 52-53 -0.42267537484692497 1107 54-55 -0.38910586293017246 1107 56-57 -0.30228299952583626 1107 58-59 -0.46805108558786657 1107 60-61 -0.6328533121704609 1107 62-63 -0.24987786794172706 1107 64-65 -0.1979602296115459 1107 66-67 -0.41445205802227747 1107 68-69 -0.4421137395120667 1107 70-71 -0.19528345112014733 1107 72-73 -0.4192016180423792 1107 74-75 -0.5235577320214375 1107 76-77 -0.7570542923708139 1107 78-79 -0.5337344326067388 1107 80-81 -0.48893404002638263 1107 82-83 -0.1051942633094356 1107 84-85 -0.26642613975228 1107 86-87 -0.39016171092623253 1107 88-89 -0.2934373593068855 1107 90-91 -0.10258413129922062 1107 92-93 -0.2367320973124727 1107 94-95 -0.17279988684719783 1107 96-97 -0.2802184086243571 1107 98-99 -0.06096537529782253 1107 100-101 0.23275915960890003 1108 1 -0.27765228736382497 1108 2 -0.3014115626063436 1108 3 -0.17891896254631234 1108 4 -0.2539253418476335 1108 5 -0.0859919205303541 1108 6 -0.22225648558242028 1108 7 -0.2505979540677288 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2316 44-45 -0.4742658258112513 2316 46-47 -0.8881213780823458 2316 48-49 -1.0905729674591598 2316 50-51 -0.6585401372626123 2316 52-53 -0.5949355497909039 2316 54-55 -0.3606684307028871 2316 56-57 -0.956578886173709 2316 58-59 -1.0487006193115676 2316 60-61 -1.3584641702634315 2316 62-63 -1.0513851331770283 2316 64-65 -0.8456505354020436 2316 66-67 -1.0688744537426516 2316 68-69 -0.664607052603948 2316 70-71 -0.741450226223165 2316 72-73 -1.1111429901236356 2316 74-75 -1.4167027475639813 2316 76-77 -0.8119682662015109 2316 78-79 -0.7750143429660206 2316 80-81 -0.4845423679378946 2316 82-83 -0.8352419753024947 2316 84-85 -1.3154935871606526 2316 86-87 -1.6076940611033486 2316 88-89 -1.8929457811965733 2316 90-91 -2.194953826954535 2316 92-93 -1.7420454783577988 2316 94-95 -1.6582151980595121 2316 96-97 -2.014287301850697 2316 98-99 -1.9644118438874294 2316 100-101 -2.1198216254702587 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1976.0 3 385.0 4 275.0 5 331.0 6 359.0 7 438.0 8 600.0 9 700.0 10 927.0 11 1261.0 12 1898.0 13 2538.0 14 2856.0 15 3773.0 16 5093.0 17 7040.0 18 5656.0 19 3688.0 20 3512.0 21 3533.0 22 3737.0 23 4176.0 24 4613.0 25 5254.0 26 5880.0 27 6800.0 28 8153.0 29 9865.0 30 11874.0 31 15025.0 32 18959.0 33 25020.0 34 34633.0 35 50243.0 36 74434.0 37 109451.0 38 97756.0 39 15086.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.746470874871314 19.306595149903096 15.482903023708246 22.46403095151735 2 14.719029315734247 20.45985178240177 40.7567264676637 24.064392434200286 3 18.11696112038909 25.36220737745744 31.413707480003517 25.10712402214995 4 11.903113806667106 16.836970136462522 38.51440437734854 32.74551167952183 5 12.683895892266989 37.53737423672334 35.165197330477056 14.613532540532622 6 30.038823512192263 36.85288047773777 18.228937970199937 14.879358039870027 7 26.74653912445246 33.68274484116384 21.756950111428665 17.81376592295504 8 25.745510833647433 33.673230406668885 20.90577416391907 19.675484595764615 9 25.95488144028103 16.26116071428571 21.293911007025763 36.490046838407494 10-11 23.99989387095623 26.594474519422988 28.229319246227615 21.176312363393166 12-13 25.448612033616396 24.29458079225059 28.66387078948765 21.592936384645363 14-15 22.249825977430575 25.298536746889315 27.917061895323027 24.534575380357083 16-17 20.878802183968617 28.879764623221497 28.10331767082382 22.138115521986066 18-19 20.613190978676073 27.023632678150655 31.346777262282842 21.016399080890423 20-21 21.56243768306315 25.977083832631116 31.296960671495295 21.163517812810444 22-23 21.174387152779367 25.941510239961506 31.34885388719714 21.535248720061983 24-25 21.22500386437993 25.9679928730773 31.341630393325275 21.465372869217493 26-27 20.861242180796275 26.33619419983863 31.23866798640982 21.56389563295528 28-29 20.550074328187538 26.60976558033162 31.512590051457977 21.32757004002287 30-31 20.710760768728285 26.290133994982735 31.25213855846064 21.746966677828343 32-33 20.618757599295172 26.539826482188488 31.018024224425883 21.823391694090457 34-35 21.16940426575141 26.473510115517048 30.78774428909632 21.56934132963523 36-37 21.194699655693498 26.133357482354747 30.953922935350143 21.718019926601613 38-39 20.91691278224785 26.49340861467035 30.882878861743258 21.706799741338546 40-41 20.924821231594947 26.574799188522235 30.981310035360384 21.519069544522434 42-43 20.854472898825513 26.743093798847372 30.31859577492228 22.083837527404835 44-45 20.844383416778804 26.836082475169903 30.144749613266136 22.174784494785154 46-47 21.15945767449859 27.07904553346176 29.67166658434091 22.08983020769874 48-49 21.348492430361983 27.1545497874427 29.268870578355287 22.228087203840026 50-51 21.136035731897323 27.337792501388343 29.461981338098077 22.06419042861626 52-53 21.499543865331844 27.491424027728595 28.89322605704749 22.115806049892072 54-55 20.96711919178611 27.28527015182373 29.191712916453945 22.555897739936213 56-57 21.20589098764495 27.53432187152174 28.959510663536363 22.300276477296944 58-59 21.499049879931892 27.161088788217413 28.96796293891615 22.371898392934543 60-61 21.374359949847687 26.831853486472866 29.833127413762988 21.96065914991646 62-63 21.14467946560529 27.117385387553227 29.632420726086128 22.105514420755352 64-65 20.91757181018644 27.284807215175867 29.55893744061353 22.238683534024158 66-67 20.874922598920183 27.13981401789577 29.231586774727873 22.75367660845618 68-69 20.84494419015131 27.11009698704553 29.583655327885722 22.461303494917438 70-71 21.022936690198748 27.129022197711546 29.241696321843495 22.60634479024621 72-73 20.9819774743584 26.969209956066493 29.26705224730458 22.78176032227053 74-75 20.649692504124946 27.330090693905323 29.065219856806802 22.954996945162932 76-77 20.91433557958016 26.981305872226457 29.159985401072603 22.944373147120782 78-79 20.900598307250576 26.90480045296094 29.147469336867747 23.04713190292074 80-81 20.850938902729457 27.22991684683795 28.998979334262543 22.92016491617005 82-83 20.849557878291446 27.015022167827883 29.23363436209983 22.901785591780843 84-85 20.782672235538783 26.961596182390014 28.80793884361462 23.447792738456585 86-87 20.80511133020064 26.90911219355157 28.911830285692336 23.373946190555454 88-89 20.686529540033074 26.491453366700323 28.94952510573532 23.87249198753128 90-91 20.47142475606499 26.24656551946175 29.60439906194321 23.677610662530046 92-93 20.716884038683347 26.147937366857928 29.609516988677264 23.525661605781465 94-95 20.28895897206998 26.334029693479582 29.234778250148175 24.142233084302262 96-97 19.99855492518994 26.536329455106188 29.36675541377156 24.098360205932305 98-99 20.300582010630404 26.668489928005567 28.851464264839993 24.179463796524033 100-101 20.10438157528935 26.42360958693648 29.176909120173246 24.295099717600923 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 851.0 1 505.5 2 167.5 3 186.5 4 157.5 5 146.0 6 276.5 7 551.5 8 888.0 9 1060.5 10 996.5 11 936.0 12 994.5 13 1058.0 14 1117.0 15 1196.5 16 1209.0 17 1253.5 18 1352.5 19 1407.0 20 1495.5 21 1667.5 22 1942.0 23 2234.5 24 2539.0 25 3080.0 26 3795.5 27 4528.5 28 5269.0 29 6038.0 30 6995.5 31 8030.5 32 9298.5 33 10338.5 34 11373.5 35 12393.5 36 13282.0 37 14457.0 38 15213.5 39 15582.0 40 16112.5 41 17014.5 42 18060.5 43 19133.5 44 19683.0 45 20147.5 46 20541.5 47 21604.5 48 24654.0 49 24335.5 50 20950.5 51 18949.0 52 17738.0 53 17258.0 54 16447.5 55 14886.0 56 12955.5 57 11221.0 58 9907.0 59 8655.5 60 7471.0 61 6130.0 62 4775.5 63 3603.0 64 2655.5 65 2015.5 66 1496.5 67 1111.0 68 770.0 69 495.0 70 386.0 71 318.0 72 243.0 73 184.5 74 137.0 75 103.5 76 71.5 77 43.5 78 27.0 79 17.0 80 10.5 81 8.5 82 6.0 83 4.5 84 5.0 85 3.5 86 3.5 87 3.5 88 0.5 89 0.5 90 0.5 91 0.5 92 0.5 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3492156794736756 2 0.3121582916361658 3 0.3108804506762517 4 0.3130710351789616 5 0.2982845897856696 6 0.22325707056785427 7 0.23019392149310242 8 0.23019392149310242 9 0.22617784990480086 10-11 0.2363093032298343 12-13 0.23366234695572646 14-15 0.21440345820273493 16-17 0.23201940857869402 18-19 0.21595512222548782 20-21 0.21869335285387526 22-23 0.21732423753968153 24-25 0.2080142534031643 26-27 0.2241698141106501 28-29 0.22572147813340296 30-31 0.23475763920708143 32-33 0.23448381614424268 34-35 0.22143158348226266 36-37 0.21778060931107943 38-39 0.20783170469460513 40-41 0.20847062517456222 42-43 0.2071015098603685 44-45 0.2253563807162846 46-47 0.21723296318540194 48-49 0.2264516729676396 50-51 0.23448381614424268 52-53 0.2481749692861798 54-55 0.23913880821250127 56-57 0.20071230506079782 58-59 0.1872949749816995 60-61 0.1943231002612272 62-63 0.19843044620380831 64-65 0.19395800284410888 66-67 0.20691896115180936 68-69 0.1965136847639371 70-71 0.20052975635223869 72-73 0.21495110432841244 74-75 0.2053672971290565 76-77 0.2160463965797674 78-79 0.2142209094941758 80-81 0.20025593328939995 82-83 0.2142209094941758 84-85 0.195235843804023 86-87 0.19934318974660414 88-89 0.21011356355159463 90-91 0.2075578816317664 92-93 0.21093503274011086 94-95 0.209383368717358 96-97 0.20345053568918528 98-99 0.2116652275743475 100-101 0.19258888752991518 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 547799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.90000706128602 #Duplication Level Percentage of deduplicated Percentage of total 1 80.18006273563067 49.63146449509894 2 11.65342890274728 14.426946627365023 3 3.594514821628958 6.675014785221892 4 1.5095712690139793 3.737698888459193 5 0.8088202134222646 2.5032988461074517 6 0.49531255676247155 1.8395910456684372 7 0.34129073040100916 1.478812903524175 8 0.24721924512773244 1.224229841527395 9 0.1690455561623449 0.9417529008115353 >10 0.9208360134512237 10.344398718062667 >50 0.05159462415780593 2.161683717948511 >100 0.025649894166690144 2.737645652783805 >500 0.0017689582183921795 0.8595634307453273 >1k 8.844791091960898E-4 1.437898146675676 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3514 0.6414761618768928 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2566 0.4684199861628079 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1799 0.32840512669793115 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 949 0.17323872442264407 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 877 0.16009521740638447 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 853 0.1557140484009646 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 822 0.1500550384356306 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 607 0.1108070660954109 No Hit CTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGC 602 0.1098943225526151 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.73823062838742E-4 0.0 24-25 0.0 0.0 0.0 3.650974171183226E-4 0.0 26-27 0.0 0.0 0.0 5.47646125677484E-4 0.0 28-29 0.0 0.0 0.0 0.0012778409599141292 0.0 30-31 0.0 0.0 0.0 0.0027382306283874197 0.0 32-33 0.0 0.0 0.0 0.00419862029686071 0.0 34-35 0.0 0.0 0.0 0.0062066560910114844 0.0 36-37 0.0 0.0 0.0 0.008579789302280582 0.0 38-39 0.0 0.0 0.0 0.01259586089058213 0.0 40-41 0.0 0.0 0.0 0.01852869391875487 0.0 42-43 0.0 0.0 0.0 0.031124554809337002 0.0 44-45 0.0 0.0 0.0 0.052665302419318036 0.0 46-47 0.0 0.0 0.0 0.07292820906938494 0.0 48-49 0.0 0.0 0.0 0.12468076794590717 0.0 50-51 0.0 0.0 0.0 0.1778937164909027 0.0 52-53 0.0 0.0 0.0 0.2485400667032981 0.0 54-55 0.0 0.0 0.0 0.37623288834043145 0.0 56-57 0.0 0.0 0.0 0.49990963838926317 0.0 58-59 0.0 0.0 0.0 0.6318010803232572 0.0 60-61 0.0 0.0 0.0 0.789888261935491 0.0 62-63 0.0 0.0 0.0 1.008399066080807 0.0 64-65 0.0 0.0 0.0 1.1534340150310607 0.0 66-67 0.0 0.0 0.0 1.4150263143963389 0.0 68-69 0.0 0.0 0.0 1.775012367675005 0.0 70-71 0.0 0.0 0.0 2.0121431400933556 0.0 72-73 0.0 0.0 0.0 2.3943088614619596 0.0 74-75 0.0 0.0 0.0 2.9210531600094196 0.0 76-77 0.0 0.0 0.0 3.298107517538367 0.0 78-79 0.0 0.0 0.0 3.65663318114856 0.0 80-81 0.0 0.0 0.0 4.193417658666774 0.0 82-83 0.0 0.0 0.0 4.821476490464568 0.0 84-85 0.0 0.0 0.0 5.492616817482325 0.0 86-87 0.0 0.0 0.0 6.4220635671112944 0.0 88-89 0.0 0.0 0.0 7.5850813893417115 9.127435427958066E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 4365 0.0 29.415583 1 ACACCGT 65 0.005842274 29.229895 6 GTACATG 2695 0.0 28.762508 1 TACACCG 70 0.0083515905 27.166924 5 GGTATCA 3065 0.0 26.997074 1 TACATGG 2780 0.0 26.849325 2 ACATGGG 2715 0.0 26.791689 3 TACACTG 455 0.0 25.07716 5 CATGGGG 1235 0.0 24.254477 4 ATCAACG 5305 0.0 23.92789 3 TATCAAC 5405 0.0 23.66111 2 TCAACGC 5370 0.0 23.551884 4 GAGTACT 2865 0.0 23.376265 12-13 CAACGCA 5445 0.0 23.22535 5 AACGCAG 5490 0.0 23.013884 6 AGTACTT 2920 0.0 22.935957 12-13 TAGCACT 230 3.5761332E-9 22.739618 4 GTACACT 360 0.0 22.452501 4 CATGGGA 1635 0.0 22.391918 4 TAGATTG 150 3.0087864E-5 22.186321 5 >>END_MODULE