FastQCFastQC Report
Thu 26 May 2016
SRR937369_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937369_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences547799
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38900.7101144762951375No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31440.5739331397100031No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23570.43026730607394315No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT18970.346294900136729No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7300.13326055724818775No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT5840.10660844579855021No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACGTA459.6116937E-442.2302174
TACGCAT500.00161357638.0071954
CTACTCG500.001615024338.0002569
GTGGACG550.002576058334.5519944
TCTACGC702.0496527E-433.9349982
GCCGTTC804.490428E-429.6876988
ACGCATT650.005836033329.2363035
CTACGCA650.005836033329.2363033
TCCTACG1601.8520695E-623.7566641
CTAGCAC1601.8535411E-623.7544983
GAGTACT30300.023.35824412-13
GTATCAA57900.023.1412091
AGTACTT30650.022.93653312-13
GTACTTT31450.022.42656114-15
GGTATCA44300.021.7724741
GTACATG29050.021.5895351
CTACGTC2257.120252E-821.1151083
AGTACAC2951.3278623E-1020.9361675
TACATGG29800.020.8848252
TAGGACC1150.003716746320.6560844
Produced by FastQC (version 0.11.5)