##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937369_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 547799 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20232238467029 33.0 31.0 34.0 31.0 34.0 2 32.41487114799406 34.0 31.0 34.0 31.0 34.0 3 32.48468507609543 34.0 31.0 34.0 31.0 34.0 4 36.10329153576403 37.0 35.0 37.0 35.0 37.0 5 35.99557136833035 37.0 35.0 37.0 35.0 37.0 6 36.08320387587418 37.0 35.0 37.0 35.0 37.0 7 36.03779671010717 37.0 35.0 37.0 35.0 37.0 8 36.02370029883224 37.0 35.0 37.0 35.0 37.0 9 37.732029448757665 39.0 37.0 39.0 35.0 39.0 10-11 37.65127355106526 39.0 37.0 39.0 35.0 39.0 12-13 37.57897969875812 39.0 37.0 39.0 35.0 39.0 14-15 38.76403662657289 40.0 38.0 41.0 35.0 41.0 16-17 38.756673524413145 40.0 38.0 41.0 35.0 41.0 18-19 38.7866443713844 40.0 38.0 41.0 35.0 41.0 20-21 38.702397229640795 40.0 38.0 41.0 34.0 41.0 22-23 38.57840649581324 40.0 38.0 41.0 34.0 41.0 24-25 38.43507290082677 40.0 38.0 41.0 34.0 41.0 26-27 38.35312861104164 40.0 38.0 41.0 34.0 41.0 28-29 38.253243434179325 40.0 38.0 41.0 34.0 41.0 30-31 38.29118344502272 40.0 38.0 41.0 34.0 41.0 32-33 38.18581085398111 40.0 37.0 41.0 34.0 41.0 34-35 38.04825310013344 40.0 37.0 41.0 34.0 41.0 36-37 37.87443021984341 40.0 37.0 41.0 33.0 41.0 38-39 37.73127460984777 40.0 37.0 41.0 33.0 41.0 40-41 37.551657633548075 40.0 37.0 41.0 33.0 41.0 42-43 37.31222035819708 40.0 36.5 41.0 33.0 41.0 44-45 36.94564247105234 40.0 36.0 41.0 31.5 41.0 46-47 36.47904340825741 40.0 36.0 41.0 30.5 41.0 48-49 36.08073216636029 39.0 36.0 41.0 29.5 41.0 50-51 35.82862235966112 39.0 35.0 41.0 29.0 41.0 52-53 35.58812812728756 39.0 35.0 41.0 28.0 41.0 54-55 35.344062329431054 39.0 35.0 40.0 27.0 41.0 56-57 35.08727927579277 38.5 35.0 40.0 26.5 41.0 58-59 34.838293790240584 38.0 34.5 40.0 26.0 41.0 60-61 34.50116739899124 38.0 34.0 40.0 26.0 41.0 62-63 34.12657471079721 37.5 34.0 40.0 25.0 41.0 64-65 33.77982526437617 37.0 33.5 40.0 23.5 41.0 66-67 33.36179511098049 36.0 33.0 39.0 22.5 41.0 68-69 32.95653423974852 36.0 33.0 39.0 22.0 40.0 70-71 32.53290623020487 35.0 32.0 38.5 20.5 40.0 72-73 32.08366572410684 35.0 32.0 38.0 19.0 40.0 74-75 31.64229215460415 35.0 31.5 37.0 18.0 39.0 76-77 30.305142032022694 34.0 29.5 35.5 15.0 38.5 78-79 30.938011935034567 34.5 31.0 36.0 15.0 38.5 80-81 30.793052743798363 35.0 31.0 36.0 12.0 37.5 82-83 30.476928581468748 34.0 31.0 35.5 7.5 37.0 84-85 30.12756777577177 34.0 31.0 35.0 4.5 37.0 86-87 29.7818670716814 34.0 30.5 35.0 2.0 36.0 88-89 29.419680393721055 34.0 30.0 35.0 2.0 36.0 90-91 29.0023503146227 34.0 29.0 35.0 2.0 35.0 92-93 28.654081150202902 34.0 29.0 35.0 2.0 35.0 94-95 28.247800744433633 34.0 29.0 35.0 2.0 35.0 96-97 27.794346101398503 33.5 28.5 35.0 2.0 35.0 98-99 27.34754262055973 33.0 27.5 35.0 2.0 35.0 100-101 25.562720085286756 31.5 22.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.19569687148888448 1101 2 0.13925014213287312 1101 3 0.12985608382493297 1101 4 0.10219848641033735 1101 5 0.01878205586020698 1101 6 0.05456169378663134 1101 7 0.031924334780271124 1101 8 0.03698635078006163 1101 9 0.10692520715387843 1101 10-11 0.12655333968098859 1101 12-13 0.054110830412014366 1101 14-15 0.08321329400710198 1101 16-17 0.08877104935162805 1101 18-19 0.11320012256618384 1101 20-21 0.11559676478193381 1101 22-23 0.017163931168511226 1101 24-25 0.01565671870478269 1101 26-27 -0.05066210912332281 1101 28-29 -0.1778344939422709 1101 30-31 0.035156381473356646 1101 32-33 0.1513307539008366 1101 34-35 0.11275767989442187 1101 36-37 0.030283898767521578 1101 38-39 0.05601007736533603 1101 40-41 0.12309441997566495 1101 42-43 0.22445557423029072 1101 44-45 0.2941629706924971 1101 46-47 0.44832566250752137 1101 48-49 0.5759493672048634 1101 50-51 0.5295982051433441 1101 52-53 0.3534915086596797 1101 54-55 0.4907362919132865 1101 56-57 0.4524658313634049 1101 58-59 0.23677839671756118 1101 60-61 0.41827883337065686 1101 62-63 0.6902046089827536 1101 64-65 0.4963978321542726 1101 66-67 0.30409537564373323 1101 68-69 0.4673245332274618 1101 70-71 0.3923424446671149 1101 72-73 0.49004348769449635 1101 74-75 0.5195970537738894 1101 76-77 0.41436483259857937 1101 78-79 0.5393072886722301 1101 80-81 0.5026253400867553 1101 82-83 0.5446833605198016 1101 84-85 0.393440000487427 1101 86-87 0.6433009717197926 1101 88-89 0.37907533681884686 1101 90-91 0.3660327783479964 1101 92-93 0.23015538221690335 1101 94-95 0.09027899760451774 1101 96-97 0.18104339574320605 1101 98-99 0.16668188689632046 1101 100-101 0.5497863212449801 1102 1 0.09369735585300987 1102 2 0.10226137332592344 1102 3 0.10930390872730555 1102 4 0.1052953895072406 1102 5 -0.06555633302363617 1102 6 0.07246954351266055 1102 7 0.1040204205127182 1102 8 0.012749981089150708 1102 9 0.0900481448222692 1102 10-11 0.1398317279593826 1102 12-13 0.1113520740168994 1102 14-15 0.10345744379670663 1102 16-17 -0.003599458927972421 1102 18-19 -0.021577221302067073 1102 20-21 0.0017174622662778916 1102 22-23 0.05973196919109114 1102 24-25 0.015905712135591443 1102 26-27 0.08477820813517667 1102 28-29 0.10218094516407916 1102 30-31 8.361562440413195E-4 1102 32-33 -0.005577489371049182 1102 34-35 0.022819587683606812 1102 36-37 0.015415433899057973 1102 38-39 -0.15361741635306458 1102 40-41 -0.028388945144065758 1102 42-43 0.08644661349405425 1102 44-45 0.10531015683968548 1102 46-47 0.1078434174798204 1102 48-49 0.1572044757327049 1102 50-51 0.219272167544581 1102 52-53 0.17561029896028657 1102 54-55 0.20905585114194736 1102 56-57 0.15220472882958092 1102 58-59 0.11019588831686633 1102 60-61 0.18011623838987845 1102 62-63 0.05422013890738242 1102 64-65 0.11428903186365602 1102 66-67 0.05376010485391447 1102 68-69 0.3971575789696047 1102 70-71 0.27184249007625283 1102 72-73 0.1938366642149454 1102 74-75 0.3065078702301598 1102 76-77 0.1950303791732182 1102 78-79 0.2698850442499854 1102 80-81 0.5363461647166652 1102 82-83 0.3162209138755365 1102 84-85 0.36739256307635415 1102 86-87 0.4313265824726784 1102 88-89 0.4124041292385492 1102 90-91 0.5857706162676486 1102 92-93 0.7449761674922364 1102 94-95 0.7466627653519211 1102 96-97 0.6061712981438347 1102 98-99 0.25448726333805993 1102 100-101 0.4469891184477781 1103 1 0.04897074100443888 1103 2 -6.820766219561847E-5 1103 3 0.01939280357229478 1103 4 0.02499966896761663 1103 5 -0.1958386523303517 1103 6 -0.008485070978537124 1103 7 -0.07997850738864543 1103 8 -0.00822294842036797 1103 9 0.06530053470880404 1103 10-11 0.01527285187611227 1103 12-13 0.04043163266962324 1103 14-15 -0.053912117536278004 1103 16-17 -0.07667526523548673 1103 18-19 0.07192988135136602 1103 20-21 -0.10211975742460311 1103 22-23 -0.10961494405825789 1103 24-25 -0.07094544483751264 1103 26-27 -0.06796792784266614 1103 28-29 -0.12111709825591532 1103 30-31 -0.08260885403024076 1103 32-33 -0.08410313039668438 1103 34-35 0.20425552979027373 1103 36-37 0.043061060630051884 1103 38-39 0.17131781102118282 1103 40-41 0.08101537005293125 1103 42-43 0.19437679307057465 1103 44-45 0.13456393152029023 1103 46-47 0.34266652120535923 1103 48-49 0.5334999282476716 1103 50-51 0.48199775496528474 1103 52-53 0.4566620723901096 1103 54-55 0.5159664202476293 1103 56-57 0.35823751717943964 1103 58-59 0.5023103795167216 1103 60-61 0.20514304085570956 1103 62-63 0.2608615665853904 1103 64-65 0.31052216864557636 1103 66-67 0.44912997888233264 1103 68-69 0.6646629394627652 1103 70-71 0.5449931199341975 1103 72-73 0.8256377899185061 1103 74-75 0.8086911544256346 1103 76-77 0.6539611841406057 1103 78-79 0.6259111319833899 1103 80-81 0.4191803021239764 1103 82-83 0.3794747332269175 1103 84-85 0.4657296934256827 1103 86-87 0.42273304186694105 1103 88-89 0.25791024913491256 1103 90-91 0.43353261037110613 1103 92-93 0.45032772650233 1103 94-95 0.7880558235132717 1103 96-97 0.6166242598163088 1103 98-99 0.47346899299052225 1103 100-101 0.463210194120073 1104 1 0.0648354131979616 1104 2 0.01630709771647787 1104 3 0.04516976700695352 1104 4 0.12158944028404761 1104 5 0.0035986499244486936 1104 6 0.10471029127064924 1104 7 -0.011553660578599079 1104 8 0.1946686371457389 1104 9 0.141835437988874 1104 10-11 -0.01938262091014309 1104 12-13 0.053880809649086814 1104 14-15 0.058159351084817956 1104 16-17 -0.09673764258339901 1104 18-19 -0.10355271707067004 1104 20-21 -0.1328979223491089 1104 22-23 0.012381331323211953 1104 24-25 0.020658143612159563 1104 26-27 -0.20194656134821543 1104 28-29 -0.08349544740886472 1104 30-31 -0.0702134837711128 1104 32-33 -0.053007071283388996 1104 34-35 -0.11329573377654611 1104 36-37 0.058597826219383364 1104 38-39 -0.16321701215955642 1104 40-41 0.06574393347157326 1104 42-43 0.27494920285870705 1104 44-45 0.19972723401538417 1104 46-47 0.3826160586317613 1104 48-49 0.09529655606616672 1104 50-51 0.08262002658209155 1104 52-53 0.2259134440910131 1104 54-55 0.4346305637856247 1104 56-57 0.605454065699611 1104 58-59 0.6635778511815928 1104 60-61 0.6173241454253713 1104 62-63 0.5443205557319288 1104 64-65 0.33785856577802065 1104 66-67 0.4575904698911799 1104 68-69 0.6304530191615925 1104 70-71 0.6155806484695816 1104 72-73 0.3688713995945072 1104 74-75 0.3149864606053008 1104 76-77 0.5472313391328001 1104 78-79 0.5343475931952923 1104 80-81 0.7593946679375847 1104 82-83 0.6116926834852343 1104 84-85 0.6218607959928626 1104 86-87 0.6906577685279984 1104 88-89 0.859811606818436 1104 90-91 0.6494153049123348 1104 92-93 0.8229097281844169 1104 94-95 0.6466880274461495 1104 96-97 0.6823207234311894 1104 98-99 0.8283315225271473 1104 100-101 1.2558655868070048 1105 1 -0.1256874260317744 1105 2 -0.1451162490516964 1105 3 -0.11022656080316295 1105 4 -0.12924090752905926 1105 5 -0.16978543725274164 1105 6 -0.12849615745304277 1105 7 -0.06297924648694675 1105 8 -0.00891585307668663 1105 9 -0.030643663369538388 1105 10-11 -0.20299211486446467 1105 12-13 -0.2025007398359122 1105 14-15 -0.08636315227389701 1105 16-17 0.03825452042600119 1105 18-19 8.378636130146333E-4 1105 20-21 -0.15332541152660184 1105 22-23 -0.12007780436867677 1105 24-25 -0.28537633914645966 1105 26-27 -0.22292450581753798 1105 28-29 -0.23327526529213571 1105 30-31 -0.1589207535128736 1105 32-33 0.04657868778513574 1105 34-35 -0.09270030908629678 1105 36-37 0.010586929070555584 1105 38-39 -0.17169309067874394 1105 40-41 -0.1643883623934812 1105 42-43 -0.42122280042535465 1105 44-45 -0.380079453549925 1105 46-47 -0.8959236487872531 1105 48-49 -0.4632896135113853 1105 50-51 -0.764306753534342 1105 52-53 -0.7520870037370244 1105 54-55 -0.6129138458277552 1105 56-57 -0.2891664000415446 1105 58-59 -0.31141375204277466 1105 60-61 -0.3907087699351237 1105 62-63 -0.4448849226976819 1105 64-65 -0.2557888069641763 1105 66-67 -0.06680682821301076 1105 68-69 -0.30337105905903883 1105 70-71 -0.21055011731635886 1105 72-73 -0.1250735921953101 1105 74-75 -0.09638090765861662 1105 76-77 -0.23265651726367764 1105 78-79 -0.1788745295095886 1105 80-81 -0.12488843401792948 1105 82-83 0.017686115340740827 1105 84-85 0.02467278285657315 1105 86-87 0.025721770617863626 1105 88-89 -0.15325937392495703 1105 90-91 0.19757547807251186 1105 92-93 -0.1394727169566501 1105 94-95 -0.4748552999161433 1105 96-97 -0.3640667970942637 1105 98-99 -0.13598684588604826 1105 100-101 0.3211878363964935 1106 1 -0.2973750469215233 1106 2 -0.16366879579573634 1106 3 -0.07028458788246894 1106 4 -0.11946495652969702 1106 5 -0.020817029773695594 1106 6 -0.28797391373930736 1106 7 -0.3548876926507134 1106 8 -0.15668342238680566 1106 9 -0.2115027865158936 1106 10-11 -0.4670636815956115 1106 12-13 -0.5902183413833058 1106 14-15 -0.468483558463447 1106 16-17 -0.15572517009623965 1106 18-19 -0.2177388610807327 1106 20-21 0.03587107461137151 1106 22-23 -0.06643902293734527 1106 24-25 -0.047610381699648485 1106 26-27 0.18755663538168932 1106 28-29 -0.06498061667833355 1106 30-31 -0.18080985731686638 1106 32-33 -0.3625605772051941 1106 34-35 -0.6956298706594737 1106 36-37 -0.14176639136787372 1106 38-39 -0.09910685599144387 1106 40-41 -0.32713417154887026 1106 42-43 -0.32073521177287745 1106 44-45 -0.2479066618026664 1106 46-47 0.0915118766801939 1106 48-49 -0.059957086109712066 1106 50-51 -0.2301433105943005 1106 52-53 -0.23037520489756247 1106 54-55 -0.10441932616301841 1106 56-57 0.04146706224084795 1106 58-59 0.19372406227057581 1106 60-61 0.09340631710549019 1106 62-63 -0.052823349519087515 1106 64-65 -0.25716292043935596 1106 66-67 -0.1917020570976078 1106 68-69 -0.020964547131249844 1106 70-71 -0.2026197498115181 1106 72-73 -0.12888564893290422 1106 74-75 0.021091690665041796 1106 76-77 -0.1492627390560699 1106 78-79 -0.26726911364885453 1106 80-81 -0.14639874994314184 1106 82-83 -0.28585998395004353 1106 84-85 -0.1630045221079648 1106 86-87 0.26121903045022776 1106 88-89 -0.1555562791473939 1106 90-91 -0.46145095648583734 1106 92-93 -0.28110714635703715 1106 94-95 -0.7261004643262012 1106 96-97 -0.08658452759071267 1106 98-99 -0.018969598948590516 1106 100-101 0.8039449285950759 1107 1 -0.11304925183699766 1107 2 -0.25925289856570544 1107 3 -0.17021321984643123 1107 4 -0.17325415319324833 1107 5 -0.27808541819423027 1107 6 -0.2007755552041388 1107 7 -0.1802367269519749 1107 8 -0.2028719232120011 1107 9 -0.24596908742137913 1107 10-11 -0.325752739983578 1107 12-13 -0.27296957917268116 1107 14-15 -0.44927886601507794 1107 16-17 -0.4606138805650417 1107 18-19 -0.280239418643518 1107 20-21 -0.25984461297504424 1107 22-23 -0.22245177997378107 1107 24-25 -0.2772526494189904 1107 26-27 -0.2970323293357424 1107 28-29 -0.24907000514348 1107 30-31 -0.38738463763713327 1107 32-33 -0.42438090817446295 1107 34-35 -0.3739741322233314 1107 36-37 -0.3665333301251792 1107 38-39 -0.6482928143303539 1107 40-41 -0.4845653683016238 1107 42-43 -0.2462359031507262 1107 44-45 -0.6448021522348881 1107 46-47 -0.5845909825017515 1107 48-49 -0.4429982565455006 1107 50-51 -0.3656622900313877 1107 52-53 -0.34568810913057746 1107 54-55 -0.6080301027364641 1107 56-57 -0.47192559577244 1107 58-59 -0.5320219567311639 1107 60-61 -0.5953376054601947 1107 62-63 -0.7348720582036918 1107 64-65 -0.6433019096895478 1107 66-67 -0.5230794601931947 1107 68-69 -0.2356061457752574 1107 70-71 -0.38012320670076605 1107 72-73 -0.4891983301408054 1107 74-75 -0.8295617729149569 1107 76-77 -0.7513636356170252 1107 78-79 -0.8089012114230663 1107 80-81 -0.6502481633870936 1107 82-83 -0.48126566662991266 1107 84-85 -0.6127748062419549 1107 86-87 -0.6206178043773711 1107 88-89 -0.5838983104601176 1107 90-91 -0.3903795438447766 1107 92-93 -0.25170351550820413 1107 94-95 -0.8312077394053752 1107 96-97 -1.0896957279118702 1107 98-99 -0.6928109834884886 1107 100-101 0.10990638985561318 1108 1 -0.13788662389273298 1108 2 -0.1376518996755891 1108 3 -0.07429741641814047 1108 4 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-0.4725103145472076 2316 64-65 -0.6613204161595831 2316 66-67 -0.8923684792057074 2316 68-69 -0.6047686557152545 2316 70-71 -0.5822847045681847 2316 72-73 -0.3355620979424394 2316 74-75 -0.3529117331549614 2316 76-77 -0.42280150158969576 2316 78-79 -0.6263666925189959 2316 80-81 -0.49081058657801435 2316 82-83 -0.7294403214408725 2316 84-85 -0.5248961826606653 2316 86-87 -0.8265836107823432 2316 88-89 -0.5901062913753208 2316 90-91 -0.5539919200466095 2316 92-93 -0.5032396971029414 2316 94-95 -0.20901804808751123 2316 96-97 -0.2318828890482827 2316 98-99 -0.29831051359764515 2316 100-101 -0.9614989452127034 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 1.0 4 4.0 5 17.0 6 23.0 7 60.0 8 122.0 9 263.0 10 498.0 11 826.0 12 1320.0 13 1909.0 14 2371.0 15 3453.0 16 5058.0 17 7396.0 18 6406.0 19 3521.0 20 2949.0 21 3018.0 22 2775.0 23 3030.0 24 3296.0 25 3998.0 26 4809.0 27 5724.0 28 7253.0 29 8950.0 30 11079.0 31 13779.0 32 17775.0 33 24077.0 34 33700.0 35 49243.0 36 77439.0 37 121239.0 38 108323.0 39 12093.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.384234374914385 20.101619445768566 15.656590561664776 20.857555617652267 2 15.803059151258033 21.82424575437341 39.602299383533015 22.770395710835544 3 19.182583392813786 26.11268001584523 31.107577779440998 23.597158811899984 4 12.890494506196614 17.83263569301879 37.836505725640244 31.440364075144352 5 13.04131890364035 38.78323871987905 33.91330863462652 14.262133741854083 6 30.557375971843687 37.716023577991194 17.270020573239456 14.45657987692566 7 27.340867727031267 34.70104910742809 20.76637598827307 17.191707177267574 8 26.544590260296204 34.565232868260075 19.821138775353734 19.069038096089987 9 26.832834671111115 16.84212640037678 20.710516083453967 35.61452284505813 10-11 24.73699294814339 27.40950604144951 27.20888501074299 20.644615999664108 12-13 26.32367787386381 25.047601835066715 27.949716397120707 20.67900389394877 14-15 22.82269591583774 26.12993086880407 27.383949222251225 23.66342399310696 16-17 21.33054277207516 29.60145965947364 27.674110394506013 21.39388717394519 18-19 21.109111188592898 27.50434009554599 31.226690811775853 20.159857904085257 20-21 22.167346052110354 26.331008271281988 31.253799294996888 20.247846381610774 22-23 21.776291409346232 26.278083144545477 31.4447245583642 20.50090088774409 24-25 22.08109178731615 26.43433084032647 31.16900541987116 20.315571952486223 26-27 21.638635903109034 26.815975435887047 31.325724592043315 20.219664068960608 28-29 21.097098021747723 27.322299128393347 31.249412402572922 20.33119044728601 30-31 21.43705994351943 26.74137776812298 31.172930217105176 20.64863207125241 32-33 21.25825348348573 26.946836339606318 31.019498027561205 20.77541214934675 34-35 21.83793690751535 26.815309995089443 30.758453374321604 20.588299723073607 36-37 21.947648681359404 26.46454265159301 30.969205858353156 20.61860280869443 38-39 21.591769974023318 26.92602578683057 31.012834999698796 20.469369239447317 40-41 21.479867615676554 27.099629608670334 30.68771574975493 20.732787025898187 42-43 21.60996602160668 27.30741773339833 30.09479624831797 20.98781999667702 44-45 21.797867465986613 27.286742034943472 29.92247156347492 20.99291893559499 46-47 22.155662934762567 27.743844001175617 29.265113663953386 20.835379400108433 48-49 22.416525039293607 27.850817544391283 28.78920918073965 20.943448235575456 50-51 22.121891423679124 27.96682724868063 28.812940512852343 21.098340814787907 52-53 22.467547403335892 28.36213647706549 28.096801929174752 21.073514190423857 54-55 21.901098760676817 28.09488516773488 28.479515296669035 21.524500774919268 56-57 22.130014841210006 28.30810205933198 28.35839422854003 21.203488870917983 58-59 22.470103085255726 27.85419469549963 28.33767495011856 21.338027269126083 60-61 22.237627304905633 27.659050126049884 29.165350794725803 20.937971774318683 62-63 21.903289345179527 27.83886060398066 29.116610289540507 21.141239761299307 64-65 21.804347945140464 28.081650386364345 28.86058572578628 21.253415942708916 66-67 21.831912800132898 27.824530530358764 28.64298766518376 21.70056900432458 68-69 21.865410488153504 27.934789950328497 28.702954915945444 21.496844645572555 70-71 22.15666695265964 28.076356473816126 28.474130109766538 21.29284646375769 72-73 21.929180034593074 27.751404487972287 28.436602941780492 21.882812535654143 74-75 21.764917424091685 28.367430389613705 28.042219865315566 21.825432320979047 76-77 21.880196933546795 27.88011661211503 28.204688215933217 22.034998238404963 78-79 21.829813489984463 27.915165964158383 28.143443124211622 22.11157742164553 80-81 21.640875576625735 28.308740979811937 28.142712929377385 21.90767051418495 82-83 21.731185583006873 28.032687231424724 28.236138138510913 21.999989047057493 84-85 21.52747479431099 28.08022373414775 28.076572719987297 22.315728751553962 86-87 21.66551965228122 27.82745130969571 27.744391647301292 22.76263739072178 88-89 21.515464613845587 27.46189752080599 27.950580413618866 23.07205745172956 90-91 21.592669441125217 27.37393755551275 28.303402469627166 22.729990533734867 92-93 21.58611297718842 27.240631480561966 28.277338843940765 22.895916698308845 94-95 21.335836684623374 27.565402638559032 27.994027006255944 23.104733670561647 96-97 21.259713873154205 27.658867577341322 28.102826036557204 22.97859251294727 98-99 21.354606401087995 27.961427352260642 27.37708733610504 23.306878910546324 100-101 20.94873459148434 27.987697835618764 27.256378164662408 23.80718940823449 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 247.0 1 169.0 2 131.5 3 175.0 4 148.0 5 147.0 6 273.5 7 574.0 8 930.5 9 1137.5 10 1077.0 11 998.0 12 1094.0 13 1216.0 14 1310.0 15 1357.0 16 1381.5 17 1441.5 18 1509.0 19 1567.0 20 1669.5 21 1814.0 22 1999.0 23 2269.0 24 2656.0 25 3107.0 26 3716.0 27 4452.5 28 5241.5 29 6062.5 30 7049.0 31 8106.5 32 9079.0 33 10174.0 34 11426.0 35 12288.0 36 13213.5 37 14349.0 38 15177.0 39 15594.5 40 15825.5 41 16909.0 42 18124.5 43 18973.5 44 19763.5 45 20580.0 46 20625.5 47 21484.0 48 24772.5 49 24207.0 50 20838.0 51 19170.5 52 17893.0 53 16965.5 54 15957.5 55 14499.5 56 12629.5 57 11112.0 58 10075.0 59 8904.0 60 7536.5 61 6062.0 62 4833.5 63 3723.5 64 2673.5 65 2037.0 66 1489.0 67 1171.0 68 874.5 69 507.0 70 342.0 71 263.5 72 205.5 73 164.5 74 131.5 75 102.5 76 64.0 77 35.5 78 23.0 79 18.5 80 9.5 81 3.5 82 1.5 83 2.0 84 2.5 85 2.5 86 1.0 87 0.0 88 0.5 89 2.0 90 1.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0485579564767369 2 0.0 3 0.0 4 0.0 5 0.024096429529809293 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0047462664225381935 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0010952922513549678 24-25 0.0 26-27 0.0014603896684732903 28-29 0.003194602399785323 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.01788977343879781 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 2.7382306283874194E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.8254870855916128E-4 84-85 0.0010952922513549678 86-87 0.0 88-89 0.0 90-91 0.011774391702065904 92-93 0.001277840959914129 94-95 0.0 96-97 0.0 98-99 2.7382306283874194E-4 100-101 0.0165206581246041 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 547799.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.319966791200194 #Duplication Level Percentage of deduplicated Percentage of total 1 78.81404173027083 46.75246338119933 2 12.208185117409792 14.483782714911467 3 3.863037194630286 6.874657142959191 4 1.6894466218165771 4.008716700066588 5 0.9018189852797849 2.6747936129235352 6 0.5675244108386825 2.0199317522487665 7 0.3679604333240775 1.5279180479681942 8 0.2790270177587685 1.3241498741838218 9 0.19347125608972887 1.0329037637665055 >10 1.0222425691453638 10.959600728828649 >50 0.06093215852287295 2.4923575875563837 >100 0.030158337918998693 3.450745743597032 >500 9.232144260917709E-4 0.33735270243703214 >1k 0.0012309525681223612 2.0606262473534738 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3890 0.7101144762951375 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3144 0.5739331397100031 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2357 0.43026730607394315 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1897 0.346294900136729 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 730 0.13326055724818775 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 584 0.10660844579855021 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 0.0 0.0 22-23 0.0 0.0 0.0 2.73823062838742E-4 0.0 24-25 0.0 0.0 0.0 3.650974171183226E-4 0.0 26-27 0.0 0.0 0.0 5.47646125677484E-4 0.0 28-29 0.0 0.0 0.0 0.0012778409599141292 0.0 30-31 0.0 0.0 0.0 0.002829504982667 0.0 32-33 0.0 0.0 0.0 0.0046549920682586125 0.0 34-35 0.0 0.0 0.0 0.006754302216688968 0.0 36-37 0.0 0.0 0.0 0.008944886719398904 0.0 38-39 0.0 0.0 0.0 0.012869683953420872 0.0 40-41 0.0 0.0 0.0 0.018802516981593614 0.0 42-43 0.0 0.0 0.0 0.03148965222645532 0.0 44-45 0.0 0.0 0.0 0.05312167419071594 0.0 46-47 0.0 0.0 0.0 0.07347585519506242 0.0 48-49 0.0 0.0 0.0 0.1260498832601009 0.0 50-51 0.0 0.0 0.0 0.1796279292222147 0.0 52-53 0.0 0.0 0.0 0.2502742794346101 0.0 54-55 0.0 0.0 0.0 0.3792449420316576 0.0 56-57 0.0 0.0 0.0 0.5023740459548118 0.0 58-59 0.0 0.0 0.0 0.6346305853059242 0.0 60-61 0.0 0.0 0.0 0.7937217848152334 0.0 62-63 0.0 0.0 0.0 1.0122325889605495 0.0 64-65 0.0 0.0 0.0 1.1576326353279214 0.0 66-67 0.0 0.0 0.0 1.4198638551731566 0.0 68-69 0.0 0.0 0.0 1.782405590371651 0.0 70-71 0.0 0.0 0.0 2.0212705755213136 0.0 72-73 0.0 0.0 0.0 2.403345022535638 0.0 74-75 0.0 0.0 0.0 2.9343792157342383 0.0 76-77 0.0 0.0 0.0 3.313624157765896 0.0 78-79 0.0 0.0 0.0 3.6760746186101105 0.0 80-81 0.0 0.0 0.0 4.21806173432226 0.0 82-83 0.0 0.0 0.0 4.8526010452739055 0.0 84-85 0.0 0.0 0.0 5.535515763993727 0.0 86-87 0.0 0.0 0.0 6.474911418239172 0.0 88-89 0.0 0.0 0.0 7.651346570548687 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTACGTA 45 9.6116937E-4 42.230217 4 TACGCAT 50 0.001613576 38.007195 4 CTACTCG 50 0.0016150243 38.000256 9 GTGGACG 55 0.0025760583 34.551994 4 TCTACGC 70 2.0496527E-4 33.934998 2 GCCGTTC 80 4.490428E-4 29.687698 8 ACGCATT 65 0.0058360333 29.236303 5 CTACGCA 65 0.0058360333 29.236303 3 TCCTACG 160 1.8520695E-6 23.756664 1 CTAGCAC 160 1.8535411E-6 23.754498 3 GAGTACT 3030 0.0 23.358244 12-13 GTATCAA 5790 0.0 23.141209 1 AGTACTT 3065 0.0 22.936533 12-13 GTACTTT 3145 0.0 22.426561 14-15 GGTATCA 4430 0.0 21.772474 1 GTACATG 2905 0.0 21.589535 1 CTACGTC 225 7.120252E-8 21.115108 3 AGTACAC 295 1.3278623E-10 20.936167 5 TACATGG 2980 0.0 20.884825 2 TAGGACC 115 0.0037167463 20.656084 4 >>END_MODULE