FastQCFastQC Report
Thu 26 May 2016
SRR937368_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937368_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797935
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT46090.5776159712257264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35170.4407627187678194No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24990.3131834046632871No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT12990.16279521514910364No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12510.15677968756853627No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT12100.15164142442680167No Hit
GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT9130.11442034752204126No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACATG36500.029.6780871
TACATGG37400.028.7080362
GTATCAA59850.028.4192621
ACATGGG37400.027.3107413
GGTATCA42450.027.1970941
CGTTCGC700.0083857427.1457126
GAGTACT37150.024.359912-13
ATCAACG69450.024.3525453
TCAACGC69500.024.3365554
CAACGCA70000.024.2999975
TATCAAC70450.024.1417432
AACGCAG70900.023.987016
CATGGGG15450.023.9861224
CATGGGA22350.023.808634
GGACCGA1050.002194288422.6214286
GTACTTT40500.022.10826514-15
ATGGGAG8750.021.7206695
ACGCAGA77650.021.533437
CGCAGAG78500.021.3607778
AGTACTT38100.020.82240112-13
Produced by FastQC (version 0.11.5)