##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937368_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 797935 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.78890511131859 33.0 31.0 34.0 30.0 34.0 2 31.886377963117297 33.0 31.0 34.0 30.0 34.0 3 31.922281890128897 33.0 31.0 34.0 30.0 34.0 4 35.547469405402694 37.0 35.0 37.0 33.0 37.0 5 35.56175377693672 37.0 35.0 37.0 33.0 37.0 6 35.59076240545909 37.0 35.0 37.0 33.0 37.0 7 35.4653023115918 37.0 35.0 37.0 33.0 37.0 8 35.48028598820706 37.0 35.0 37.0 33.0 37.0 9 37.117441896896366 39.0 37.0 39.0 34.0 39.0 10-11 37.00842487170007 39.0 37.0 39.0 33.0 39.0 12-13 36.868705470997014 39.0 37.0 39.0 33.0 39.0 14-15 38.02514991822642 40.0 38.0 41.0 33.0 41.0 16-17 37.97506689141346 40.0 38.0 41.0 33.0 41.0 18-19 37.976037521853286 40.0 38.0 41.0 33.0 41.0 20-21 37.87827955911196 40.0 38.0 41.0 33.0 41.0 22-23 37.72894784662911 40.0 37.5 41.0 32.5 41.0 24-25 37.66275009869225 40.0 37.0 41.0 32.0 41.0 26-27 37.65276244305613 40.0 37.0 41.0 32.5 41.0 28-29 37.56834829904692 40.0 37.0 41.0 32.0 41.0 30-31 37.33850501607274 39.5 37.0 41.0 32.0 41.0 32-33 37.09404337446033 39.0 36.0 41.0 31.0 41.0 34-35 37.11992956819791 39.5 36.0 41.0 31.0 41.0 36-37 36.997143877634144 39.5 36.0 41.0 31.0 41.0 38-39 36.87560891551317 39.0 36.0 41.0 31.0 41.0 40-41 36.66277328353813 39.0 35.5 41.0 30.5 41.0 42-43 36.382504214002395 39.0 35.0 41.0 30.0 41.0 44-45 36.112563679999 39.0 35.0 41.0 29.5 41.0 46-47 35.70842361846516 39.0 35.0 41.0 27.5 41.0 48-49 35.352769962465615 39.0 35.0 40.5 26.5 41.0 50-51 34.33485872909448 38.0 34.0 39.5 25.0 40.5 52-53 34.4391322601465 38.0 34.0 39.5 24.5 40.5 54-55 34.81330747491964 38.0 35.0 40.0 25.0 41.0 56-57 34.600990055580965 38.0 34.0 40.0 23.5 41.0 58-59 34.34298909059008 38.0 34.0 40.0 23.0 41.0 60-61 34.03794294021443 38.0 34.0 40.0 22.5 41.0 62-63 33.71778340341005 37.5 33.5 40.0 22.0 41.0 64-65 33.28618120523602 37.0 33.0 40.0 20.0 41.0 66-67 32.94272716449335 36.0 32.5 39.0 18.5 41.0 68-69 32.46678488849342 36.0 32.0 39.0 16.5 40.5 70-71 31.98005351313077 35.0 31.5 38.5 12.0 40.0 72-73 31.526001491349547 35.0 31.0 38.0 8.0 40.0 74-75 31.0601778340341 35.0 31.0 37.0 7.0 39.0 76-77 30.590886475715443 35.0 30.0 36.5 3.5 39.0 78-79 30.082256073489695 34.0 30.0 36.0 2.0 39.0 80-81 29.591857106155263 34.0 29.0 36.0 2.0 37.5 82-83 29.12438481831227 34.0 29.0 35.0 2.0 37.0 84-85 28.62400258166392 34.0 29.0 35.0 2.0 36.5 86-87 28.194184363387997 34.0 27.5 35.0 2.0 36.0 88-89 27.752549393120994 33.5 26.5 35.0 2.0 36.0 90-91 27.265446433606748 33.0 26.0 35.0 2.0 35.0 92-93 26.778817823506927 33.0 25.0 35.0 2.0 35.0 94-95 26.242958386334728 32.5 24.5 35.0 2.0 35.0 96-97 25.539040147380426 32.0 21.5 34.5 2.0 35.0 98-99 24.65963643655185 32.0 10.0 34.0 2.0 35.0 100-101 22.18531772638122 29.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.23897996852481285 1101 2 -0.18214263563740474 1101 3 -0.3094383275069319 1101 4 -0.08976971852015936 1101 5 -0.209147903253303 1101 6 -0.16180939544005213 1101 7 -0.11746609060213586 1101 8 -0.21110891883053284 1101 9 -0.28734832363416984 1101 10-11 -0.3252659358673142 1101 12-13 -0.32871226959591837 1101 14-15 -0.37344715346608126 1101 16-17 -0.4197167184670221 1101 18-19 -0.209047172379357 1101 20-21 -0.09251715240769443 1101 22-23 -0.19015524390088956 1101 24-25 -0.19249373430199768 1101 26-27 -0.24380049904814882 1101 28-29 -0.2684571977636949 1101 30-31 -0.4324156002936306 1101 32-33 -0.6162410336790813 1101 34-35 -0.45728094625503246 1101 36-37 -0.4938928331353978 1101 38-39 -0.45868500735142703 1101 40-41 -0.4966688309468381 1101 42-43 -0.5066389995333793 1101 44-45 -0.5304790777736343 1101 46-47 -0.39885282972235103 1101 48-49 -0.26245775797622173 1101 50-51 -0.32175032909855616 1101 52-53 -0.324826669115879 1101 54-55 -0.20008248393975236 1101 56-57 -0.20495277276775425 1101 58-59 -0.2686303041564386 1101 60-61 -0.233719364514819 1101 62-63 -0.40073124036736374 1101 64-65 -0.42650504036637216 1101 66-67 -0.47706228752866764 1101 68-69 -0.22384875664612736 1101 70-71 -0.34873255871817577 1101 72-73 -0.19382281682581137 1101 74-75 -0.26259204387967827 1101 76-77 -0.05562029870036511 1101 78-79 -0.29673452587666915 1101 80-81 -0.1997735905011382 1101 82-83 0.022746654373126063 1101 84-85 -0.3355970429400905 1101 86-87 -0.3303547490601879 1101 88-89 -0.29434720757000576 1101 90-91 -0.38829815878145624 1101 92-93 -0.1501656699373406 1101 94-95 -0.5027777134784976 1101 96-97 -0.4213198566449883 1101 98-99 -0.39995811685403027 1101 100-101 -0.06694178459736122 1102 1 -0.17212204502754247 1102 2 -0.1958584826319374 1102 3 -0.19867330018452733 1102 4 -0.26149102999556817 1102 5 -0.1064156628161399 1102 6 -0.2670279746750239 1102 7 -0.1627939594545964 1102 8 -0.18747503904911156 1102 9 -0.32775815969974076 1102 10-11 -0.17090801237005593 1102 12-13 -0.30584341713689867 1102 14-15 -0.18198540483219716 1102 16-17 -0.13658830316648363 1102 18-19 -0.2098941669148786 1102 20-21 -0.2518750759049624 1102 22-23 -0.3816989597478795 1102 24-25 -0.3255401823894246 1102 26-27 -0.31627317664377586 1102 28-29 -0.2902194928456652 1102 30-31 -0.322579534719857 1102 32-33 -0.30826289160258113 1102 34-35 -0.14478094625503246 1102 36-37 -0.17651578395506817 1102 38-39 -0.14100741172300957 1102 40-41 -0.09302128996322523 1102 42-43 -0.22689856237490602 1102 44-45 -0.0885801706698075 1102 46-47 -0.050273594749675965 1102 48-49 -0.09576376890517935 1102 50-51 -0.03944158592916125 1102 52-53 0.058124150556253085 1102 54-55 -0.0038119921364767606 1102 56-57 0.02156362067486839 1102 58-59 0.05511286524247083 1102 60-61 0.11353473384583879 1102 62-63 0.03534799460530991 1102 64-65 -0.025207226158720175 1102 66-67 0.013060663291007302 1102 68-69 0.004539221495953427 1102 70-71 -0.28079540024823046 1102 72-73 -0.22502500261816039 1102 74-75 -0.26003739907093504 1102 76-77 -0.28175417848178697 1102 78-79 -0.24315529090398869 1102 80-81 -0.225361022195127 1102 82-83 -0.07372875546294111 1102 84-85 0.00624721935499295 1102 86-87 -0.11926185288532665 1102 88-89 -0.2769291747831204 1102 90-91 -0.41657684730605027 1102 92-93 -0.45770665354389806 1102 94-95 -0.2149498446260374 1102 96-97 0.045169214393261825 1102 98-99 -0.08704828078845495 1102 100-101 0.3128806197742229 1103 1 -0.06488767502030512 1103 2 -0.10591397684185466 1103 3 -0.04033784334826507 1103 4 -0.02453085477623773 1103 5 -0.11776932515086003 1103 6 -0.12075856484704417 1103 7 -0.014477897278432295 1103 8 -0.08545336314014662 1103 9 -0.13603163047427813 1103 10-11 -0.10943892248318576 1103 12-13 -0.13612244542196095 1103 14-15 -0.2797618444660728 1103 16-17 -0.1594116621031887 1103 18-19 -0.13925381955024108 1103 20-21 -0.11448266672010021 1103 22-23 -0.1372931259797383 1103 24-25 -0.14630111438474813 1103 26-27 -0.3144391273865992 1103 28-29 -0.1602876572897287 1103 30-31 -0.31086993284632314 1103 32-33 -0.2509060662596809 1103 34-35 -0.07570567110972348 1103 36-37 -0.06296904868914766 1103 38-39 -0.29560702018952156 1103 40-41 -0.07397574385894501 1103 42-43 0.0371410783688404 1103 44-45 -0.16686790936164897 1103 46-47 0.12757179805792163 1103 48-49 0.1330411438694057 1103 50-51 -0.13352060248439557 1103 52-53 -0.15519081953269165 1103 54-55 0.08783005200811544 1103 56-57 0.026550060591866043 1103 58-59 0.0036335926534505347 1103 60-61 0.07613150490795562 1103 62-63 0.009281743336266857 1103 64-65 -0.0984966434290655 1103 66-67 0.08501631848266555 1103 68-69 0.08042057633126376 1103 70-71 0.24424095209955965 1103 72-73 0.14201438937239175 1103 74-75 0.3071954844842537 1103 76-77 0.36269230519959805 1103 78-79 0.20774982694031152 1103 80-81 0.2752899325722389 1103 82-83 0.2024100488228946 1103 84-85 0.2817542815190208 1103 86-87 0.37644361523583925 1103 88-89 0.41478142817037167 1103 90-91 0.5719789886867197 1103 92-93 0.4558910457923524 1103 94-95 -0.03020584807218185 1103 96-97 -0.011197906905270116 1103 98-99 0.3403847985334778 1103 100-101 0.8656514371812847 1104 1 -0.13496623635534277 1104 2 -0.1540853650391476 1104 3 -0.2567600222056754 1104 4 -0.09887393397683297 1104 5 -0.04924264457754646 1104 6 -0.18380109226781371 1104 7 -0.14980694646758508 1104 8 -0.09995020049968417 1104 9 -0.2667218599339378 1104 10-11 -0.1014376944366191 1104 12-13 -0.15238775394763593 1104 14-15 -0.13733367677883024 1104 16-17 -0.20078042934630957 1104 18-19 -0.2160971510891727 1104 20-21 -0.12934118902681035 1104 22-23 -0.29827026412656465 1104 24-25 -0.22677811795113456 1104 26-27 -0.441184467538676 1104 28-29 -0.3267720796031668 1104 30-31 -0.4334348679112523 1104 32-33 -0.40955538326392116 1104 34-35 -0.12461514893759329 1104 36-37 -0.21233652059547836 1104 38-39 -0.22964998499673328 1104 40-41 -0.12868900339733358 1104 42-43 -0.15471596355296668 1104 44-45 -0.0013192417080603036 1104 46-47 0.16452885007326046 1104 48-49 0.012947021670363767 1104 50-51 -0.14558868336724373 1104 52-53 0.006648474592871878 1104 54-55 -0.008821838847140384 1104 56-57 0.022428534449616677 1104 58-59 -0.019930602219027094 1104 60-61 0.2393796880719421 1104 62-63 0.21546739706078455 1104 64-65 0.31607000753654546 1104 66-67 0.19044117212642675 1104 68-69 0.011500668518870327 1104 70-71 0.10954905720688402 1104 72-73 0.20656253552675352 1104 74-75 0.19461894185589657 1104 76-77 0.007118151374147175 1104 78-79 0.2270894575169855 1104 80-81 0.19020565156826663 1104 82-83 0.019143024396544916 1104 84-85 0.3083549476922265 1104 86-87 0.39411991561939175 1104 88-89 0.27860494273870273 1104 90-91 0.2744826055361891 1104 92-93 0.35752115143800367 1104 94-95 0.5901385096426459 1104 96-97 0.6347188382666538 1104 98-99 0.6982867735015184 1104 100-101 0.9629320710551816 1105 1 -0.26039432905659154 1105 2 -0.20903160027839007 1105 3 -0.15210900214822942 1105 4 -0.3450425810197686 1105 5 -0.24085325513900813 1105 6 -0.1879285810361324 1105 7 -0.309060500097317 1105 8 -0.21989134158921786 1105 9 -0.1901454354132781 1105 10-11 -0.21152426429397764 1105 12-13 -0.17330522258485814 1105 14-15 -0.3337867983264715 1105 16-17 -0.14145429942708176 1105 18-19 -0.1928796431717572 1105 20-21 -0.27450450869390863 1105 22-23 -0.20790664617551613 1105 24-25 -0.26322353689891287 1105 26-27 -0.19428238568858092 1105 28-29 -0.5205431006426195 1105 30-31 -0.5350125199378652 1105 32-33 -0.3306037347234252 1105 34-35 -0.3146982817782842 1105 36-37 -0.3799463210030112 1105 38-39 -0.2422487992259832 1105 40-41 -0.3063006757514515 1105 42-43 -0.15955387139181454 1105 44-45 -0.11160988443018027 1105 46-47 -0.1996222988723133 1105 48-49 -0.14892495559622176 1105 50-51 0.04907151229721052 1105 52-53 -0.07288393770183177 1105 54-55 0.029693462014563465 1105 56-57 0.12339703664684976 1105 58-59 0.24954224534928215 1105 60-61 0.21662709803437252 1105 62-63 0.16551097352212452 1105 64-65 0.03993333702369739 1105 66-67 0.13043531078550075 1105 68-69 0.1797602798904805 1105 70-71 0.18501995917826264 1105 72-73 0.46165054770361635 1105 74-75 0.5248057682620058 1105 76-77 0.3786274313191562 1105 78-79 0.2528018712846922 1105 80-81 0.5788325020334888 1105 82-83 0.6520335861032684 1105 84-85 0.8566434975166963 1105 86-87 0.7820624315155946 1105 88-89 0.5113331667830892 1105 90-91 0.8006246463875897 1105 92-93 0.9592415010316984 1105 94-95 0.9858020379857564 1105 96-97 1.1964631549140883 1105 98-99 1.1210636067816573 1105 100-101 0.9295024807966676 1106 1 -0.25985453612896237 1106 2 -0.09662676858972574 1106 3 -0.17651457957491345 1106 4 0.14219749459459763 1106 5 -0.054440428445055034 1106 6 -0.11167253275392852 1106 7 -0.4483444181701941 1106 8 -0.3538597084229522 1106 9 -0.6909518730400919 1106 10-11 -0.8303524150371189 1106 12-13 -0.366237837350468 1106 14-15 -0.5602158404866842 1106 16-17 -0.37975770207358295 1106 18-19 -0.639228778635939 1106 20-21 -0.32915800065555345 1106 22-23 -0.4719976262140193 1106 24-25 -0.36117210698962054 1106 26-27 -0.5670408359409151 1106 28-29 -0.2105740574418391 1106 30-31 -0.5489105619419874 1106 32-33 -0.47973291215704705 1106 34-35 -0.33914288038045726 1106 36-37 -0.9083927579361202 1106 38-39 -0.7103630792419438 1106 40-41 -0.3971083707272527 1106 42-43 -0.3541232076849852 1106 44-45 -0.26995456280897656 1106 46-47 -0.3191517468527465 1106 48-49 -0.12101505982612082 1106 50-51 -0.3342540890624619 1106 52-53 -0.21180365813678748 1106 54-55 -0.15863790580770853 1106 56-57 -0.10275018591259055 1106 58-59 -0.19413827527991856 1106 60-61 -0.34711724389517684 1106 62-63 -0.26546165095542307 1106 64-65 0.15867694188660408 1106 66-67 0.05726843889635802 1106 68-69 -0.006032543681776303 1106 70-71 0.3806417832881408 1106 72-73 0.08603054458192005 1106 74-75 0.31397435708287347 1106 76-77 0.4397703615492965 1106 78-79 0.2902112554438325 1106 80-81 -0.16786804079441353 1106 82-83 -0.054947735760730865 1106 84-85 -0.2846987875633431 1106 86-87 -0.30917449639061445 1106 88-89 0.4448334962952387 1106 90-91 0.5000835527541092 1106 92-93 0.6868357588489395 1106 94-95 0.5778291446956629 1106 96-97 0.7424983866998751 1106 98-99 0.5976411461930446 1106 100-101 0.8174239094919749 1107 1 0.07403286379544483 1107 2 -0.05477970814922273 1107 3 -0.048947547539270886 1107 4 0.01142346213516987 1107 5 -0.007563817786362392 1107 6 0.10983956794588323 1107 7 0.1575868145778614 1107 8 0.007990158142924031 1107 9 0.039602823497489226 1107 10-11 0.15121878609907213 1107 12-13 0.17022389626232837 1107 14-15 0.15405330702387943 1107 16-17 0.1768122105356582 1107 18-19 0.07305388981748706 1107 20-21 0.021076511250441854 1107 22-23 0.11474887185605098 1107 24-25 0.22687508745769236 1107 26-27 0.16029683829655994 1107 28-29 0.013462124395083208 1107 30-31 0.15613559595696103 1107 32-33 0.1690912354107894 1107 34-35 0.06422905149368319 1107 36-37 -0.15061578190844926 1107 38-39 0.08310537761818182 1107 40-41 0.3100903430365065 1107 42-43 0.13242815013608578 1107 44-45 -0.0033089420825902494 1107 46-47 0.3150433246748108 1107 48-49 0.019330580371338613 1107 50-51 -0.01157043102033839 1107 52-53 -0.17068819466795304 1107 54-55 -0.1906749810233208 1107 56-57 0.06487142239812016 1107 58-59 -0.07147838340164725 1107 60-61 0.21846376722624683 1107 62-63 0.2165111410820515 1107 64-65 0.3474046217363309 1107 66-67 0.1329121296957041 1107 68-69 0.18366725817141827 1107 70-71 0.35191908574263664 1107 72-73 0.5605964541674133 1107 74-75 0.6488968736619185 1107 76-77 0.33764958934940736 1107 78-79 0.49192227607171546 1107 80-81 0.6372837763149306 1107 82-83 0.8056765985003445 1107 84-85 0.615571373588427 1107 86-87 0.6541003378189139 1107 88-89 0.5501197497169912 1107 90-91 0.5162760415009764 1107 92-93 0.5392643458216462 1107 94-95 0.8188309314530429 1107 96-97 0.7955005113376536 1107 98-99 0.8970353953540702 1107 100-101 1.4543520898628408 1108 1 0.0572450849320667 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0.03153504218597192 2316 7 0.25899748439567105 2316 8 -0.024749665740323223 2316 9 0.25748555030288856 2316 10-11 0.17479893657942824 2316 12-13 0.2755868449828469 2316 14-15 0.4557717472089493 2316 16-17 0.49958721771250936 2316 18-19 0.38395536991922796 2316 20-21 0.6184147315232309 2316 22-23 0.3199736243379121 2316 24-25 0.3782400256699532 2316 26-27 0.2762047608799634 2316 28-29 0.4796641312448102 2316 30-31 0.45001623980456174 2316 32-33 0.2399385052005556 2316 34-35 0.36418832699823867 2316 36-37 0.39366042363676 2316 38-39 0.48258263532412116 2316 40-41 0.7380747475575902 2316 42-43 0.6128069547052419 2316 44-45 0.43969011658070656 2316 46-47 0.0344916079036679 2316 48-49 -0.06862765365431045 2316 50-51 -0.6304292104871791 2316 52-53 -0.2821931984399768 2316 54-55 -0.17624448972567563 2316 56-57 -0.15423303927077825 2316 58-59 -0.4220027947323999 2316 60-61 -0.37714533102660397 2316 62-63 -0.9038030383861226 2316 64-65 -0.7877306036169998 2316 66-67 -0.5816892709598918 2316 68-69 -0.5047569709005337 2316 70-71 -0.46519788702535436 2316 72-73 -0.21914411076812712 2316 74-75 -0.1468631055085048 2316 76-77 -0.19976687536381732 2316 78-79 0.4353352558363177 2316 80-81 0.028202213829533918 2316 82-83 0.11886132080601541 2316 84-85 0.4066647261490637 2316 86-87 0.21597820438944737 2316 88-89 0.31505929720776393 2316 90-91 0.7554556765827947 2316 92-93 0.8700307007122596 2316 94-95 1.0393841169764855 2316 96-97 1.2451323218419041 2316 98-99 2.041463817046207 2316 100-101 1.459733064179705 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3085.0 3 653.0 4 452.0 5 493.0 6 639.0 7 736.0 8 894.0 9 1180.0 10 1493.0 11 2005.0 12 2767.0 13 3892.0 14 4270.0 15 5477.0 16 7270.0 17 9984.0 18 8522.0 19 5452.0 20 5396.0 21 5693.0 22 6037.0 23 6559.0 24 7422.0 25 8399.0 26 9789.0 27 11266.0 28 13501.0 29 16177.0 30 19767.0 31 24287.0 32 30660.0 33 40424.0 34 54519.0 35 76567.0 36 110999.0 37 153885.0 38 122073.0 39 15250.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.8863354450981 19.29078887672631 15.371728827788328 22.451146850387268 2 14.824028112283294 20.72112479285785 40.61631934629142 23.838527748567437 3 18.26334480233249 25.40377032355642 31.158871390185904 25.174013483925183 4 12.04016487043651 16.846959683123142 38.33074336165343 32.782132084786916 5 12.885949715511858 37.6960292264611 34.85139907360762 14.566621984419422 6 30.403066193333544 36.81228990606641 18.05460086079608 14.730043039803963 7 27.136986817799112 33.74894756022469 21.38205762971713 17.73200799225907 8 26.033156502006626 33.94068318376804 20.650188097936578 19.375972216288755 9 26.35162979565991 16.25033922666372 21.010191876652158 36.38783910102421 10-11 24.217012884283964 26.681846083477996 28.0304015571468 21.070739475091244 12-13 25.6771980309061 24.42895334554331 28.355077767899495 21.538770855651094 14-15 22.388212531252748 25.457640747301895 27.53301169700853 24.62113502443682 16-17 21.111459463602785 28.99852288770114 27.79101028575306 22.099007362943016 18-19 20.963900683690127 27.18583895117675 30.93391581747239 20.916344547660735 20-21 21.94310992064888 26.1260672700501 30.795672122579642 21.135150686721378 22-23 21.55527372568825 26.023177470528786 30.860872989999226 21.560675813783742 24-25 21.50349738198848 26.16730148839733 30.84536395887561 21.48383717073857 26-27 21.19025707869403 26.440692871837555 30.896995775554753 21.472054273913656 28-29 20.917255395943343 26.83405530547432 30.976782758111586 21.271906540470752 30-31 21.14312568363688 26.337792169361634 30.82722497300007 21.69185717400141 32-33 20.978828549511565 26.533766324332376 30.688079994019045 21.799325132137017 34-35 21.583687605480524 26.503054301343493 30.393848419692798 21.519409673483185 36-37 21.513668685142843 26.153106861981456 30.60058041659338 21.73264403628232 38-39 21.283105765709514 26.55188723986027 30.482210528894505 21.682796465535706 40-41 21.296686551170303 26.478194151229843 30.597282583775275 21.62783671382458 42-43 21.22160479324784 26.75396662868285 29.952836411195133 22.071592166874172 44-45 21.203083573124914 26.7836723923451 29.860146011082765 22.153098023447217 46-47 21.569940737985494 27.103261170060367 29.287762569930685 22.039035522023454 48-49 21.629311086913223 27.118196778273674 29.071000001885004 22.1814921329281 50-51 21.53790955343493 27.32366966412581 29.000495771490186 22.137925010949072 52-53 21.816023283832354 27.509777537839053 28.571213093447685 22.102986084880907 54-55 21.317548653755168 27.1896153379734 28.945002233740347 22.547833774531085 56-57 21.570553812059188 27.52112506187388 28.598672820971537 22.309648305095394 58-59 21.875786999907678 27.13132920633984 28.648900539540445 22.343983254212038 60-61 21.719665022341264 26.99044490986564 29.32756406432023 21.962326003472864 62-63 21.587083662015708 27.07270872796153 29.257399749782692 22.082807860240067 64-65 21.308501131353147 27.326935624969778 29.09012579591934 22.274437447757737 66-67 21.327665165259464 27.267309098125715 28.797814962657416 22.60721077395741 68-69 21.174052250720052 27.282153637063622 29.08614623619717 22.457647876019156 70-71 21.41208981001727 27.310346993248547 28.747872507458 22.529690689276183 72-73 21.326708520810254 27.165185693218675 28.80681775359315 22.701288032377924 74-75 21.10094047982434 27.538083340556195 28.650718580851493 22.710257598767967 76-77 21.305161723966805 27.083252193905434 28.70834406467702 22.903242017450733 78-79 21.252894927101543 27.10719600203265 28.79261360959643 22.847295461269375 80-81 21.276137369301377 27.531644973327968 28.497465952565044 22.69475170480561 82-83 21.2859043171224 27.2619076031046 28.715791430570437 22.736396649202558 84-85 21.292282531280502 27.149816041391627 28.380310085504014 23.17759134182386 86-87 21.26554211721402 27.176053440700482 28.479031685662154 23.079372756423343 88-89 21.239963594771368 26.759852997244483 28.489038546454037 23.511144861530113 90-91 20.994926995469722 26.74357834010488 28.922595850639087 23.33889881378631 92-93 21.27236081041274 26.592476605195444 28.888398978222213 23.24676360616961 94-95 20.915810180358303 26.651588655440015 28.674244612992126 23.758356551209552 96-97 20.695637753788056 26.755080786292275 28.871378875838737 23.677902584080936 98-99 20.853565842290077 26.795735939845844 28.399407001652104 23.951291216211974 100-101 20.77658391252441 26.422668492947654 28.833070507132103 23.967677087395835 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1313.0 1 793.0 2 271.5 3 260.5 4 230.0 5 225.0 6 359.0 7 749.0 8 1196.5 9 1424.5 10 1343.0 11 1258.5 12 1324.0 13 1363.0 14 1452.0 15 1568.0 16 1642.0 17 1718.5 18 1794.5 19 1906.0 20 2030.5 21 2232.0 22 2567.5 23 2991.5 24 3533.0 25 4238.0 26 5246.5 27 6399.5 28 7455.0 29 8526.5 30 10015.0 31 11594.5 32 13152.5 33 14737.0 34 16558.0 35 17910.5 36 19007.0 37 20670.5 38 22033.0 39 22648.5 40 23243.0 41 24776.5 42 26235.5 43 27283.0 44 28288.5 45 29445.5 46 30078.5 47 31517.5 48 35482.0 49 35236.0 50 30937.5 51 28227.0 52 26433.5 53 25458.0 54 24250.5 55 21927.5 56 19291.0 57 16888.0 58 15149.0 59 13329.0 60 11178.5 61 9282.5 62 7306.0 63 5491.5 64 4142.0 65 3161.0 66 2366.0 67 1802.5 68 1288.0 69 834.0 70 611.0 71 480.5 72 381.0 73 298.5 74 224.0 75 179.0 76 128.0 77 76.0 78 43.5 79 27.5 80 21.5 81 15.5 82 9.5 83 6.0 84 3.5 85 3.0 86 3.0 87 2.0 88 1.5 89 2.0 90 2.5 91 3.0 92 1.5 93 0.0 94 0.0 95 0.0 96 0.5 97 0.5 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2799726794789049 2 0.24864180666345 3 0.2351068696071735 4 0.2401198092576463 5 0.24362886701297723 6 0.2706987411255303 7 0.27132535858183937 8 0.259795597385752 9 0.2515242469624719 10-11 0.2657484632206884 12-13 0.26787896257213933 14-15 0.25127359997994825 16-17 0.2663750806769975 18-19 0.2548453194809101 20-21 0.2480151892071409 22-23 0.24394217574113178 24-25 0.23949319180133719 26-27 0.24720058651393909 28-29 0.26098617055273926 30-31 0.2631166699041902 32-33 0.2601089061139065 34-35 0.2722026230206721 36-37 0.24500742541685724 38-39 0.24369152875860817 40-41 0.2408090884595863 42-43 0.2483911596809264 44-45 0.2638059491061302 46-47 0.2690695357391266 48-49 0.2733931961876594 50-51 0.27640095997794306 52-53 0.29595142461478696 54-55 0.27376916666144485 56-57 0.2457593663644282 58-59 0.22489300506933524 60-61 0.220882653348957 62-63 0.22896601853534437 64-65 0.22113330033148065 66-67 0.2247050198324425 68-69 0.22708616616641708 70-71 0.2268355191838934 72-73 0.23021925344796257 74-75 0.23209910581688986 76-77 0.24814051269840273 78-79 0.2419369998809427 80-81 0.2351695313528044 82-83 0.22884069504408253 84-85 0.22959263599165347 86-87 0.23968117703822991 88-89 0.237550677686779 90-91 0.2452580723993809 92-93 0.23454291389649531 94-95 0.23222442930815168 96-97 0.2438795139955009 98-99 0.24814051269840273 100-101 0.22326379968293156 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 797935.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 57.40644616579619 #Duplication Level Percentage of deduplicated Percentage of total 1 77.3357334455429 44.39569618733922 2 12.732240109202001 14.618253127977912 3 4.313344362925139 7.428413128944072 4 1.8827379856426498 4.323251872683772 5 0.9998244957751421 2.869818554598001 6 0.6073312825940986 2.0918838347425246 7 0.40818039769341763 1.640253021828432 8 0.2805958225075378 1.2886407183301016 9 0.20549273592254286 1.0616946913979666 >10 1.123677873372108 11.651387729566828 >50 0.07447922982359383 2.8888967271194095 >100 0.03395271171935826 3.488142592026079 >500 0.0010952487634403115 0.4447730753352052 >1k 0.0013142985161283735 1.8088947381105251 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4609 0.5776159712257264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3517 0.4407627187678194 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2499 0.3131834046632871 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1299 0.16279521514910364 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1251 0.15677968756853627 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 1210 0.15164142442680167 No Hit GCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTTACTCTGCGT 913 0.11442034752204126 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 1.8798523689272936E-4 0.0 22-23 0.0 0.0 0.0 4.3863221941636854E-4 0.0 24-25 0.0 0.0 0.0 5.012939650472783E-4 0.0 26-27 0.0 0.0 0.0 8.146026932018272E-4 0.0 28-29 0.0 0.0 0.0 0.0019425141145582034 0.0 30-31 0.0 0.0 0.0 0.004323660448532775 0.0 32-33 0.0 0.0 0.0 0.007770056458232814 0.0 34-35 1.2532349126181957E-4 0.0 0.0 0.010527173265992843 0.0 36-37 1.2532349126181957E-4 0.0 0.0 0.012532349126181957 0.0 38-39 1.2532349126181957E-4 0.0 0.0 0.016166730372774725 0.0 40-41 1.2532349126181957E-4 0.0 0.0 0.021117008277616596 0.0 42-43 1.2532349126181957E-4 0.0 0.0 0.030516270122253067 0.0 44-45 1.2532349126181957E-4 0.0 0.0 0.04718429446007507 0.0 46-47 1.2532349126181957E-4 0.0 0.0 0.06541886243866982 0.0 48-49 1.2532349126181957E-4 0.0 0.0 0.10627432059002301 0.0 50-51 1.2532349126181957E-4 0.0 0.0 0.14806970492583982 0.0 52-53 1.2532349126181957E-4 0.0 0.0 0.20064290951017313 0.0 54-55 1.2532349126181957E-4 0.0 0.0 0.296578042071096 0.0 56-57 1.2532349126181957E-4 0.0 0.0 0.3919492189213407 0.0 58-59 1.2532349126181957E-4 0.0 0.0 0.5023592147230038 0.0 60-61 1.2532349126181957E-4 0.0 0.0 0.6283719851867633 0.0 62-63 1.2532349126181957E-4 0.0 0.0 0.7958041695125543 0.0 64-65 1.2532349126181957E-4 0.0 0.0 0.9280204527937739 0.0 66-67 1.2532349126181957E-4 0.0 0.0 1.1301672441990889 0.0 68-69 1.2532349126181957E-4 0.0 0.0 1.3998007356488937 0.0 70-71 1.2532349126181957E-4 0.0 0.0 1.5994410572289723 0.0 72-73 1.2532349126181957E-4 0.0 0.0 1.891382130123381 0.0 74-75 1.2532349126181957E-4 0.0 0.0 2.2750599986214413 0.0 76-77 1.2532349126181957E-4 0.0 0.0 2.5632413667779956 0.0 78-79 1.2532349126181957E-4 0.0 0.0 2.854242513487941 0.0 80-81 1.2532349126181957E-4 0.0 0.0 3.2743268561975603 0.0 82-83 1.2532349126181957E-4 0.0 0.0 3.7657202654351547 0.0 84-85 1.2532349126181957E-4 0.0 0.0 4.2744083164668805 0.0 86-87 1.2532349126181957E-4 0.0 0.0 4.981922086385483 0.0 88-89 1.2532349126181957E-4 0.0 0.0 5.798467293701868 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTACATG 3650 0.0 29.678087 1 TACATGG 3740 0.0 28.708036 2 GTATCAA 5985 0.0 28.419262 1 ACATGGG 3740 0.0 27.310741 3 GGTATCA 4245 0.0 27.197094 1 CGTTCGC 70 0.00838574 27.145712 6 GAGTACT 3715 0.0 24.3599 12-13 ATCAACG 6945 0.0 24.352545 3 TCAACGC 6950 0.0 24.336555 4 CAACGCA 7000 0.0 24.299997 5 TATCAAC 7045 0.0 24.141743 2 AACGCAG 7090 0.0 23.98701 6 CATGGGG 1545 0.0 23.986122 4 CATGGGA 2235 0.0 23.80863 4 GGACCGA 105 0.0021942884 22.621428 6 GTACTTT 4050 0.0 22.108265 14-15 ATGGGAG 875 0.0 21.720669 5 ACGCAGA 7765 0.0 21.53343 7 CGCAGAG 7850 0.0 21.360777 8 AGTACTT 3810 0.0 20.822401 12-13 >>END_MODULE