FastQCFastQC Report
Thu 26 May 2016
SRR937368_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937368_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences797935
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54020.6769974997963493No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44080.5524259494821007No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31660.3967741733349208No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT22690.2843590016730686No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9720.12181443350648861No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGATCG804.4872696E-429.6933177
GTACATG40400.024.9384421
GTATAGG2900.024.5814861
GGTATCA61000.023.9179171
TACATGG42050.023.2686442
ACATGGG41150.023.0850053
TACACGA1452.334173E-522.9297812
GTATCAA79650.022.3748631
GAGTACT46450.022.24601712-13
GTAGTAT1503.0198118E-522.177961
AGTACTT47700.021.9120512-13
ACTAGAC2005.3846634E-721.373833
GTACTTT49200.020.70770614-15
ATACAGT3900.020.7039956
GTAGGAC1856.5510685E-620.5394153
CATGGGG16450.020.211664
CATGGGA24400.019.660814
TACTTTT54250.019.26161814-15
ATCAACG94850.018.7786243
TCAACGC94900.018.768734
Produced by FastQC (version 0.11.5)