FastQCFastQC Report
Thu 26 May 2016
SRR937367_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937367_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885005
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT73560.8311817447359056No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51990.5874543081677505No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39370.44485624375003535No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15670.17706114654719465No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12060.13627041655131894No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11800.13333258004192067No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10270.11604454212123096No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9380.10598810176213694No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA77250.034.4521641
GGTATCA49150.032.1026951
CGTATAG753.0681887E-431.6836871
GTACATG63350.029.2580221
TACATGG64450.028.45082
TATCAAC94350.027.7924792
ATCAACG94300.027.6057153
TCAACGC94800.027.4601154
CAACGCA95450.027.277755
ACATGGG65700.026.9695423
AACGCAG97050.026.867866
ATGGGAT17350.025.741415
GAGTACT52650.024.62960612-13
TGCACCG1551.406901E-624.5223585
TACCTGG5700.024.16872
ACGCAGA107350.024.0714577
CGCAGAG108050.023.8715488
CATGGGA47400.023.5515584
GCAGAGT110150.023.1576949
GTACTTT56100.023.07000414-15
Produced by FastQC (version 0.11.5)