##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937367_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 885005 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.769542544957375 33.0 31.0 34.0 30.0 34.0 2 31.87276569058932 33.0 31.0 34.0 30.0 34.0 3 31.896940695250308 33.0 31.0 34.0 30.0 34.0 4 35.53652352246598 37.0 35.0 37.0 33.0 37.0 5 35.54847825718499 37.0 35.0 37.0 33.0 37.0 6 35.60614007830464 37.0 35.0 37.0 33.0 37.0 7 35.54089411924227 37.0 35.0 37.0 33.0 37.0 8 35.530629770453274 37.0 35.0 37.0 33.0 37.0 9 37.12224337715606 39.0 37.0 39.0 34.0 39.0 10-11 37.00811181857729 39.0 37.0 39.0 33.0 39.0 12-13 36.86397534477206 39.0 37.0 39.0 33.0 39.0 14-15 37.9361325642228 40.0 38.0 41.0 33.0 41.0 16-17 37.88334190202315 40.0 38.0 41.0 33.0 41.0 18-19 37.85572736877192 40.0 38.0 41.0 33.0 41.0 20-21 37.74637318433229 40.0 37.5 41.0 33.0 41.0 22-23 37.81144626301546 40.0 38.0 41.0 33.0 41.0 24-25 37.77523347325722 40.0 37.5 41.0 33.0 41.0 26-27 37.67809560397964 40.0 37.0 41.0 33.0 41.0 28-29 37.52287275213134 40.0 37.0 41.0 32.0 41.0 30-31 37.222897045779405 39.0 36.5 41.0 32.0 41.0 32-33 36.92577951536997 39.0 35.5 41.0 31.0 41.0 34-35 36.88833678905769 39.0 36.0 41.0 31.0 41.0 36-37 36.72257501370049 39.0 35.0 41.0 30.5 41.0 38-39 36.55856803068909 39.0 35.0 41.0 30.0 41.0 40-41 36.28844413308399 39.0 35.0 41.0 30.0 41.0 42-43 35.96331885130593 39.0 35.0 40.0 29.5 41.0 44-45 35.60654572573036 39.0 35.0 40.0 28.0 41.0 46-47 35.06827249563562 38.0 35.0 40.0 25.0 41.0 48-49 34.63042864164609 38.0 34.0 40.0 24.0 41.0 50-51 33.63866136349512 37.5 33.0 39.5 22.5 40.5 52-53 33.728037129733735 38.0 33.5 39.5 22.0 40.5 54-55 34.01700837848374 38.0 34.0 40.0 21.5 41.0 56-57 33.74052236993011 38.0 33.5 40.0 19.5 41.0 58-59 33.44724945056808 38.0 33.0 40.0 18.0 41.0 60-61 33.09724238846108 37.0 33.0 40.0 15.5 41.0 62-63 32.696761600216945 36.5 32.0 39.5 12.0 41.0 64-65 32.26396913011791 36.0 31.5 39.0 8.0 41.0 66-67 31.8014451895752 35.5 31.0 39.0 7.0 40.0 68-69 31.291175191100614 35.0 31.0 38.5 2.0 40.0 70-71 30.802603940090734 35.0 30.0 38.0 2.0 40.0 72-73 30.303834441613326 35.0 29.5 37.0 2.0 39.5 74-75 29.74193818114022 34.0 29.0 36.5 2.0 39.0 76-77 29.221455810984118 34.0 29.0 36.0 2.0 39.0 78-79 28.659395144660202 34.0 27.5 35.5 2.0 38.0 80-81 28.060190055423416 34.0 26.5 35.0 2.0 37.0 82-83 27.486837927469338 33.0 26.0 35.0 2.0 36.5 84-85 26.88932943881673 33.0 25.0 35.0 2.0 36.0 86-87 26.27202897158773 32.5 24.0 35.0 2.0 36.0 88-89 25.684980310845702 32.0 21.5 35.0 2.0 35.0 90-91 25.067767413743425 32.0 19.0 34.0 2.0 35.0 92-93 24.368545375449855 31.0 13.5 34.0 2.0 35.0 94-95 23.564383252072023 31.0 4.5 34.0 2.0 35.0 96-97 22.675110310111243 30.5 2.0 34.0 2.0 35.0 98-99 21.477457189507405 30.0 2.0 34.0 2.0 35.0 100-101 18.47043745515562 24.0 2.0 32.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.025225152274490625 1101 2 0.08871291953721183 1101 3 -0.0921169517504552 1101 4 0.019676772377131613 1101 5 0.07813633388320795 1101 6 0.04453873532356312 1101 7 0.1288969924184613 1101 8 0.08099501693114064 1101 9 0.0865518188790162 1101 10-11 0.24429887607891487 1101 12-13 0.1826649606579238 1101 14-15 0.30694856968377593 1101 16-17 0.34006040528472425 1101 18-19 0.37609328002807985 1101 20-21 0.33715518716247317 1101 22-23 0.28928943771312987 1101 24-25 0.2686405262228462 1101 26-27 0.21254478202543226 1101 28-29 0.19351561280420526 1101 30-31 0.19520164756391267 1101 32-33 0.15924133039815302 1101 34-35 0.24702257379725978 1101 36-37 0.15263210019374895 1101 38-39 0.07088766073062658 1101 40-41 0.06722726076112195 1101 42-43 0.12309224963296117 1101 44-45 0.2897822606076019 1101 46-47 0.2778141567490877 1101 48-49 0.23040076473019866 1101 50-51 0.21021088199972127 1101 52-53 0.2419814474039299 1101 54-55 0.2639994563871042 1101 56-57 0.2809650213688357 1101 58-59 0.44657048722584847 1101 60-61 0.4460846566993908 1101 62-63 0.6076807302794336 1101 64-65 0.526950825143139 1101 66-67 0.6503415868754381 1101 68-69 0.59149222038495 1101 70-71 0.44811457849938563 1101 72-73 0.49213468472934707 1101 74-75 0.5855017722378513 1101 76-77 0.5678566564634373 1101 78-79 0.9887897093713569 1101 80-81 1.0210881491289108 1101 82-83 0.9209327019139444 1101 84-85 1.0484895259973719 1101 86-87 1.0507169551698752 1101 88-89 1.3039987227960061 1101 90-91 1.6185223164491518 1101 92-93 1.7407928298422455 1101 94-95 1.95089716239125 1101 96-97 1.5396459056954228 1101 98-99 2.14762056678509 1101 100-101 2.032560795161107 1102 1 0.07839865107206023 1102 2 0.16600185166872805 1102 3 0.13266596822824894 1102 4 0.05179323374331091 1102 5 0.010812705663724387 1102 6 -0.07609130157545252 1102 7 -0.058198265782849035 1102 8 -0.04334988788408367 1102 9 0.003696826497467498 1102 10-11 0.02803173455925645 1102 12-13 -0.011531063060239433 1102 14-15 0.20186274734977872 1102 16-17 0.22912287660079045 1102 18-19 0.18938169814348527 1102 20-21 0.13208588677512978 1102 22-23 0.19584130744675576 1102 24-25 0.23294798129232674 1102 26-27 0.2862598335188338 1102 28-29 0.11514173950917694 1102 30-31 0.17966918193873482 1102 32-33 0.2285729258674678 1102 34-35 0.3671914648074903 1102 36-37 0.5212910688571242 1102 38-39 0.3431770518050996 1102 40-41 0.5820702104793298 1102 42-43 0.7441691134003321 1102 44-45 0.8444548506981064 1102 46-47 0.9683026556384462 1102 48-49 1.0247576020624578 1102 50-51 0.8227102853738728 1102 52-53 0.9060392375017798 1102 54-55 1.156132806854309 1102 56-57 1.1349796708281943 1102 58-59 1.1645103840554754 1102 60-61 0.9601253741190305 1102 62-63 0.9046443636390791 1102 64-65 1.0732677160799327 1102 66-67 1.4066197430261944 1102 68-69 1.2458255598375167 1102 70-71 1.1879199866832586 1102 72-73 1.4365471459486692 1102 74-75 1.4609310674850384 1102 76-77 1.1552158986568166 1102 78-79 1.1651368905436819 1102 80-81 1.4086232582655462 1102 82-83 1.3007516948095095 1102 84-85 1.2144884612496973 1102 86-87 1.3681068547531545 1102 88-89 1.2704668813332631 1102 90-91 1.4136835890061974 1102 92-93 1.6428195219649488 1102 94-95 1.8309144186466426 1102 96-97 1.3364035654534696 1102 98-99 1.5876044119928068 1102 100-101 1.4661069556442818 1103 1 0.31788643006884243 1103 2 0.29600095240432367 1103 3 0.3150511771273692 1103 4 0.28012078180899636 1103 5 0.23015840088503836 1103 6 0.3445299102732591 1103 7 0.37297527426139965 1103 8 0.2279452327072704 1103 9 0.30123320174251944 1103 10-11 0.21794990149869875 1103 12-13 0.21920723301357015 1103 14-15 0.28035198315489396 1103 16-17 0.25233648786677776 1103 18-19 0.2591624574823399 1103 20-21 0.16582412889307108 1103 22-23 0.06595695284882197 1103 24-25 0.09289402542822955 1103 26-27 0.1317525404845341 1103 28-29 0.036808975709966774 1103 30-31 0.06829888284340058 1103 32-33 0.0691711165474942 1103 34-35 0.16612457752129473 1103 36-37 0.216673885712936 1103 38-39 0.3258834779485227 1103 40-41 0.38033858694738143 1103 42-43 0.18915139789162083 1103 44-45 0.46485448379822714 1103 46-47 0.302950233919411 1103 48-49 0.2832781322687552 1103 50-51 0.46171569894556796 1103 52-53 0.3536905387373679 1103 54-55 0.7510113162336367 1103 56-57 0.6731466102717363 1103 58-59 0.9010244755286507 1103 60-61 0.7658198760164723 1103 62-63 0.8880259574953016 1103 64-65 0.7931245531871838 1103 66-67 1.1910772168126407 1103 68-69 1.328611840659807 1103 70-71 1.3725922048088286 1103 72-73 1.316967282277151 1103 74-75 1.1997825269911608 1103 76-77 1.0407819855977003 1103 78-79 1.2067203153022312 1103 80-81 1.2721706764774474 1103 82-83 1.4060814550656175 1103 84-85 1.7064127072164972 1103 86-87 1.6552391005760718 1103 88-89 1.5171433799573855 1103 90-91 1.2780504169409497 1103 92-93 1.5777091822228577 1103 94-95 1.6476684338621972 1103 96-97 2.029532106307048 1103 98-99 1.9814933708244844 1103 100-101 1.9464450790593268 1104 1 0.074708531175812 1104 2 0.11915851488905815 1104 3 -0.08723209275377286 1104 4 -0.01451356279584104 1104 5 -0.006980550399106278 1104 6 0.007541500296454728 1104 7 -0.02240932671045215 1104 8 0.10585255720125986 1104 9 0.017553277495998998 1104 10-11 0.20201443032026134 1104 12-13 0.10420563680008854 1104 14-15 0.30696659092236445 1104 16-17 0.2506570406827038 1104 18-19 0.06969912391119948 1104 20-21 0.0679528218933072 1104 22-23 0.23253209847177203 1104 24-25 0.004086904946902337 1104 26-27 -0.008756763314167415 1104 28-29 -0.04701349076054129 1104 30-31 0.15308159963477408 1104 32-33 0.1934221032812644 1104 34-35 0.2917145099182292 1104 36-37 0.3686615137973561 1104 38-39 0.364694165588638 1104 40-41 0.404459066114093 1104 42-43 0.47671087756205566 1104 44-45 0.47895340867818703 1104 46-47 0.5189445812338462 1104 48-49 0.7204460532060963 1104 50-51 0.6557359877917435 1104 52-53 0.5381638135116447 1104 54-55 0.7840651860964769 1104 56-57 0.7704972562157266 1104 58-59 0.6883176794065449 1104 60-61 0.6991201320592069 1104 62-63 0.7025257733722583 1104 64-65 0.7427923029729726 1104 66-67 0.9242000517454265 1104 68-69 0.7477573223667697 1104 70-71 0.7464308063672824 1104 72-73 0.8650345668189594 1104 74-75 0.8104491244765697 1104 76-77 0.777844909558123 1104 78-79 1.151242348453028 1104 80-81 1.2231849386268685 1104 82-83 1.3340839398259199 1104 84-85 1.274815319097371 1104 86-87 1.638635958161398 1104 88-89 1.419162896279481 1104 90-91 1.3501614326876137 1104 92-93 1.524751647266502 1104 94-95 1.4940175729518437 1104 96-97 1.4736646345434536 1104 98-99 1.5220735854161056 1104 100-101 1.6088520536834956 1105 1 0.05400768201138817 1105 2 -0.1407304720328426 1105 3 0.015542489644211344 1105 4 -0.008178892987153574 1105 5 0.14961415507374198 1105 6 0.06497345732076809 1105 7 0.038488531578487084 1105 8 0.07387021803433669 1105 9 0.13016342502947253 1105 10-11 0.10639464452859926 1105 12-13 0.01937481819962983 1105 14-15 0.25278163613543114 1105 16-17 0.10326577356260458 1105 18-19 0.18692327577053192 1105 20-21 0.32036424782688044 1105 22-23 0.2168670437656317 1105 24-25 0.19686882120684857 1105 26-27 0.07899605947813626 1105 28-29 0.08577817916354746 1105 30-31 0.029618080944594283 1105 32-33 0.0032639971301264836 1105 34-35 0.09818096587596159 1105 36-37 0.29987882320070725 1105 38-39 0.4585426561905024 1105 40-41 0.40384480863969685 1105 42-43 0.39663403953616694 1105 44-45 0.4658540888287419 1105 46-47 0.560918097429763 1105 48-49 0.4213849364487814 1105 50-51 0.2577209701271954 1105 52-53 0.29008457802022036 1105 54-55 0.36003692280711164 1105 56-57 0.471164749337909 1105 58-59 0.5063351541010022 1105 60-61 0.4414465670949639 1105 62-63 0.4402106555651244 1105 64-65 0.6381455382484802 1105 66-67 0.7178210809431356 1105 68-69 0.5017114652542283 1105 70-71 0.6935949843604838 1105 72-73 0.7751813557414771 1105 74-75 0.6681539607304181 1105 76-77 0.5732573369175249 1105 78-79 0.6241111542248134 1105 80-81 0.6983287307174635 1105 82-83 0.7575035297997523 1105 84-85 0.865974106515889 1105 86-87 0.9095562647689484 1105 88-89 0.6912584821880436 1105 90-91 0.9567645143987491 1105 92-93 0.8092786876250777 1105 94-95 0.8661109251968604 1105 96-97 0.6797297756179219 1105 98-99 1.1526569066504315 1105 100-101 1.1937052089193934 1106 1 -0.15772021125702196 1106 2 -0.6199695274288572 1106 3 -0.18524100388726694 1106 4 -0.016707452919504817 1106 5 -0.038943081212060804 1106 6 -0.06171556764236641 1106 7 -0.056362388148151865 1106 8 -0.16704791547001463 1106 9 0.06117269378245993 1106 10-11 -0.3603403769786695 1106 12-13 -0.23862657885349137 1106 14-15 -0.09308500656619145 1106 16-17 -0.2902808214267978 1106 18-19 -0.03844426766270459 1106 20-21 -0.18464798660026815 1106 22-23 -0.1871991160948312 1106 24-25 -0.11556862798657619 1106 26-27 -0.1266370874452818 1106 28-29 0.07068101861273846 1106 30-31 0.04503550162506542 1106 32-33 0.05196722955156474 1106 34-35 0.08718509576105049 1106 36-37 0.00835672974520918 1106 38-39 0.04354884489070798 1106 40-41 0.23229730679125993 1106 42-43 3.7953077190877593E-4 1106 44-45 0.19772038022460237 1106 46-47 0.23337082433855727 1106 48-49 0.5525923253074865 1106 50-51 0.46571766948707705 1106 52-53 0.5697929942440112 1106 54-55 0.7435304542877859 1106 56-57 0.34124044347314 1106 58-59 0.17253259610491511 1106 60-61 0.3572863716709307 1106 62-63 0.5954669657926104 1106 64-65 0.5247869326872276 1106 66-67 0.7765451057632937 1106 68-69 0.7566728420459903 1106 70-71 0.7880688631436605 1106 72-73 0.47222017502734914 1106 74-75 0.4728001835808122 1106 76-77 0.5469118563026569 1106 78-79 0.45857955917414017 1106 80-81 0.2589367347697369 1106 82-83 0.35101581120382974 1106 84-85 0.28227183405785183 1106 86-87 0.3615809705503814 1106 88-89 0.5742859208014472 1106 90-91 0.6441710200911288 1106 92-93 0.6403296228745283 1106 94-95 0.2943943063921992 1106 96-97 0.6473328491347772 1106 98-99 0.6886465472156225 1106 100-101 0.2741705664955454 1107 1 -0.044124428289247675 1107 2 -0.06801613636566373 1107 3 -0.2351100926299452 1107 4 -0.021095549488990173 1107 5 -0.3467387028706881 1107 6 -0.06993257896362337 1107 7 -0.0707574004553777 1107 8 -0.2153883997110171 1107 9 7.24442488589716E-4 1107 10-11 -0.09754931064855299 1107 12-13 -0.0894295534917191 1107 14-15 0.14830951623920186 1107 16-17 -0.04265690865219085 1107 18-19 0.07734556358980882 1107 20-21 -6.127145957677271E-4 1107 22-23 -0.045374667829598536 1107 24-25 -0.05958381473762131 1107 26-27 0.13937841983366184 1107 28-29 0.09907256085708838 1107 30-31 0.06239234846673014 1107 32-33 0.15784508403537956 1107 34-35 0.04643668054503536 1107 36-37 -0.061102449111096746 1107 38-39 0.02180694997856847 1107 40-41 -0.03949641260398806 1107 42-43 -0.15181765311180584 1107 44-45 -0.11762724447206807 1107 46-47 -0.10283288855902839 1107 48-49 -0.3703551203116504 1107 50-51 -0.3646481678136553 1107 52-53 -0.21444731109365023 1107 54-55 -0.11736218106121754 1107 56-57 -0.21340259471322298 1107 58-59 -0.2789152667230823 1107 60-61 -0.20378246692063584 1107 62-63 -0.19805384151960936 1107 64-65 -0.2728280087903059 1107 66-67 -0.16773225190229013 1107 68-69 0.027651305707006912 1107 70-71 0.15856818986087973 1107 72-73 0.2771850476087998 1107 74-75 0.07706364009092681 1107 76-77 0.2295766102539467 1107 78-79 0.18206981181631576 1107 80-81 0.18402640419050087 1107 82-83 0.0856544247116453 1107 84-85 -0.06758610588846281 1107 86-87 -0.08678398878264204 1107 88-89 0.198056722452165 1107 90-91 0.08888823261331424 1107 92-93 0.3053636005734468 1107 94-95 0.19512298475465073 1107 96-97 0.24759101345061296 1107 98-99 0.5307451861914672 1107 100-101 0.8140771604859971 1108 1 -0.014218067693697378 1108 2 -0.017727098618657067 1108 3 -0.008969607046530825 1108 4 -9.767571813625864E-4 1108 5 -0.10699711585488103 1108 6 0.010237225196760846 1108 7 0.11035674077155733 1108 8 -0.00935170294912524 1108 9 0.07025324476911976 1108 10-11 0.1359958366062486 1108 12-13 0.22548214657977184 1108 14-15 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2316 20-21 0.019614870029251108 2316 22-23 -0.2972115953205332 2316 24-25 -0.27332928073405327 2316 26-27 -0.23869819073382104 2316 28-29 -0.2406278158588364 2316 30-31 -0.2538536715237001 2316 32-33 -0.2123589801423975 2316 34-35 -0.2789356617416132 2316 36-37 -0.23757092199260654 2316 38-39 -0.3783847125088471 2316 40-41 -0.6226888176377585 2316 42-43 -0.5116173211041684 2316 44-45 -0.36358406015339995 2316 46-47 -0.713875641345517 2316 48-49 -0.9495461335569644 2316 50-51 -0.851770236990987 2316 52-53 -0.6372166590913011 2316 54-55 -0.7770426917464945 2316 56-57 -0.8862720503995334 2316 58-59 -0.7942929740043141 2316 60-61 -0.861135230864754 2316 62-63 -0.7448692507170733 2316 64-65 -0.8617751480430158 2316 66-67 -0.8097636798850125 2316 68-69 -0.47431391513249466 2316 70-71 -0.858320851943315 2316 72-73 -0.9177761339340655 2316 74-75 -0.4437266858130542 2316 76-77 -0.2526071369226237 2316 78-79 -0.20502523800426786 2316 80-81 -0.1376477851316018 2316 82-83 0.06349855218080336 2316 84-85 0.27850658411204776 2316 86-87 0.4183409417251269 2316 88-89 0.7471017145407437 2316 90-91 0.537904261506533 2316 92-93 1.0597741703903019 2316 94-95 0.8212275710474763 2316 96-97 0.9199472957426345 2316 98-99 1.2317682284544098 2316 100-101 1.6782572687966848 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3526.0 3 587.0 4 560.0 5 648.0 6 738.0 7 891.0 8 1126.0 9 1505.0 10 2028.0 11 2551.0 12 3822.0 13 5231.0 14 5719.0 15 7625.0 16 10329.0 17 14090.0 18 11271.0 19 7238.0 20 7051.0 21 7615.0 22 8186.0 23 9231.0 24 10529.0 25 11877.0 26 13750.0 27 15907.0 28 18542.0 29 22281.0 30 26604.0 31 32800.0 32 40606.0 33 51702.0 34 67891.0 35 92261.0 36 121844.0 37 144521.0 38 93640.0 39 8682.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.991256463833714 19.501192145783047 15.054142579048394 21.45340881133485 2 14.729775822661594 20.703909816866503 41.286682508547024 23.27963185192488 3 18.575924014228068 26.934744204124506 30.128580671991806 24.360751109655617 4 11.828368821650386 16.925609945416245 38.099619602106294 33.14640163082707 5 12.563796901487612 38.68369881516803 33.81315100035355 14.939353282990817 6 29.757984583057222 37.34842177402455 17.95207267825486 14.941520964663372 7 27.33570010965409 34.12383209330566 20.966134104234825 17.57433369280542 8 26.57182796722106 34.15427940939914 19.902193343166108 19.37169928021369 9 27.2949429514348 16.33919396738755 20.644998294944084 35.72086478623357 10-11 24.329897491240445 27.50866041813603 27.16258359463295 20.998858495990575 12-13 26.0729155455354 25.1486690873661 27.960551285028863 20.817864082069644 14-15 22.997841474390235 25.68203688694276 27.43128655716015 23.88883508150686 16-17 20.755362415039286 29.34519437108541 27.84208138671518 22.05736182716013 18-19 20.748505994990634 27.146862405437254 31.597590111733965 20.50704148783815 20-21 21.810156575632917 25.956794458237436 31.5918862470778 20.64116271905185 22-23 21.358912661472953 26.102512496793334 31.521706506713755 21.016868335019957 24-25 21.06128135282225 26.474382570973898 31.576801318390068 20.887534757813782 26-27 20.84026570472345 26.50517875041764 31.722267211063105 20.932288333795807 28-29 20.553145213362484 26.472070867836468 32.00544875788711 20.96933516091394 30-31 20.763294175226957 26.542013298423743 31.721634919861646 20.973057606487654 32-33 20.545013764118135 26.59743069794841 31.585479138581444 21.27207639935201 34-35 21.028243038645776 26.616980940777584 31.40832298214044 20.946453038436204 36-37 21.187128445401683 26.318680789682187 31.302814767661747 21.191375997254383 38-39 20.91551528878822 26.61053227633069 31.297395243488108 21.176557191392977 40-41 20.769566152905337 26.677859189382012 31.38353170333964 21.169042954373012 42-43 20.710110776420326 26.807939023982776 30.83793320795171 21.64401699164519 44-45 20.82197661057819 26.86380030818177 30.533439873691915 21.780783207548133 46-47 21.2177145805522 27.257409441108187 29.963882355140036 21.560993623199572 48-49 21.098128551693513 27.413282462896433 29.454141558545277 22.03444742686478 50-51 21.20377162894377 27.614691112200013 29.356336228994184 21.825201029862036 52-53 21.348634186914577 27.864150721037806 28.9123921502859 21.87482294176172 54-55 21.008648487605004 27.72404136819312 29.04235657551836 22.224953568683514 56-57 21.276545143519172 27.96765120744552 28.635884124640924 22.11991952439438 58-59 21.378958536919544 27.419736548589498 29.101891808955266 22.099413105535696 60-61 21.42378301497932 27.5476625100217 29.31346281893908 21.715091656059897 62-63 21.442711135018826 27.553636646754054 29.374706265394522 21.62894595283259 64-65 21.28198406663681 27.605392499622077 29.356579065818682 21.756044367922428 66-67 21.29965534208445 27.76458171370914 28.91570631091462 22.020056633291794 68-69 21.26348236767618 27.720580283256414 29.088224186245082 21.927713162822325 70-71 21.32815658438754 27.785824374809607 28.956141830992777 21.92987720981008 72-73 21.363267792955522 27.67333168746277 28.772120160630926 22.19128035895078 74-75 21.06289269713566 28.18257387349433 28.637219194650935 22.11731423471907 76-77 21.009329979056535 27.76521119597487 28.97503887963228 22.250419945336315 78-79 21.075836493965962 27.629885152511736 28.916206470627216 22.378071882895092 80-81 21.053787992044832 28.136538052071014 28.5452257664098 22.26444818947435 82-83 21.0907060717402 27.86157037077302 28.5678289668512 22.479894590635578 84-85 21.005554520754863 27.67035665899908 28.464158875938466 22.85992994430759 86-87 21.067993047259385 27.662792080216963 28.64285673843994 22.626358134083716 88-89 21.146663586307803 27.281164285050973 28.67763138523285 22.894540743408374 90-91 20.94015738936144 27.259909878792755 28.95497676757267 22.844955964273137 92-93 21.008004122392006 27.21361974897577 29.060994980081713 22.71738114855051 94-95 20.692601522253415 26.96926388934502 29.053953815995552 23.284180772406014 96-97 20.703064389902476 26.993971339227972 29.038030509123402 23.26493376174615 98-99 20.712826678249424 27.088931462196808 28.62813964774898 23.570102211804787 100-101 21.13174792897331 26.355304438516935 29.288459153878517 23.22448847863124 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1772.0 1 1058.0 2 471.5 3 566.0 4 469.0 5 467.0 6 788.0 7 1627.0 8 2613.0 9 2944.0 10 2520.0 11 2037.0 12 1956.5 13 2050.5 14 2143.5 15 2184.5 16 2158.5 17 2110.0 18 2192.0 19 2380.0 20 2527.5 21 2705.5 22 3000.0 23 3498.0 24 4132.0 25 4880.0 26 5852.0 27 6935.0 28 7836.5 29 9092.5 30 10925.0 31 12604.0 32 14522.0 33 16830.0 34 19542.5 35 21647.0 36 24297.5 37 27121.5 38 27927.0 39 27993.5 40 27838.0 41 28085.0 42 28398.0 43 28998.5 44 30270.5 45 31443.0 46 31262.0 47 31240.0 48 34130.0 49 34092.0 50 31160.5 51 28547.0 52 25793.5 53 24855.0 54 24324.5 55 22985.5 56 21044.5 57 19221.0 58 17651.5 59 15735.5 60 13574.5 61 11575.5 62 9391.5 63 6875.5 64 4836.5 65 3548.5 66 2661.0 67 2091.0 68 1596.0 69 1140.5 70 839.5 71 605.5 72 456.0 73 357.0 74 255.0 75 187.0 76 126.5 77 98.0 78 75.0 79 48.0 80 24.0 81 17.5 82 12.0 83 8.0 84 7.5 85 5.5 86 5.0 87 3.0 88 4.5 89 4.0 90 1.0 91 1.0 92 2.5 93 2.0 94 1.0 95 1.0 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3369472488855995 2 0.2863260659544296 3 0.28519612883543033 4 0.2833882294450314 5 0.2844051728521308 6 0.2497161032988514 7 0.25186298382495015 8 0.2534448957915492 9 0.26395331099824293 10-11 0.2709024242800888 12-13 0.26440528584584266 14-15 0.2515804995452003 16-17 0.24525285167880406 18-19 0.23271054965791155 20-21 0.2383037383969582 22-23 0.23485743018401029 24-25 0.23841673210885814 26-27 0.23389698363286082 28-29 0.25254094609634975 30-31 0.2648572606934424 32-33 0.25706069457234704 34-35 0.2549703109021983 36-37 0.2422020214575059 38-39 0.2265523923593652 40-41 0.22316258100236724 42-43 0.23734329184580877 44-45 0.2355353924554099 46-47 0.2480776944763024 48-49 0.2530494177998994 50-51 0.26694764436359114 52-53 0.28553510997113013 54-55 0.25417935491889876 56-57 0.22649589550341526 58-59 0.21265416579567348 60-61 0.21706092055977086 62-63 0.22372754956186688 64-65 0.21350161863492298 66-67 0.2209592036203185 68-69 0.22073321619651867 70-71 0.22078971305246864 72-73 0.22451850554516645 74-75 0.22700436720696493 76-77 0.24316246800865532 78-79 0.24163705289800622 80-81 0.2324280653781617 82-83 0.22276710301071748 84-85 0.2090948638708256 86-87 0.21468805260987225 88-89 0.22497048039276613 90-91 0.22282359986666744 92-93 0.228529782317614 94-95 0.22282359986666744 96-97 0.23231507166626175 98-99 0.23542239874350993 100-101 0.21790837339902033 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 885005.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.51840136020412 #Duplication Level Percentage of deduplicated Percentage of total 1 76.60281324084576 40.99660104344342 2 12.887840749294039 13.794732677742244 3 4.247623921103693 6.819781255104942 4 1.9902281408895848 4.260553137700067 5 1.0674401197778587 2.85638443791279 6 0.6883182254093565 2.2102614630600836 7 0.43043136672702204 1.6125199049762582 8 0.32673507392303813 1.3989071059735296 9 0.24556520974783536 1.1828031709848625 >10 1.3516253761219583 13.290278464345715 >50 0.10928170998654348 3.998534114654812 >100 0.048920228005994756 4.548069984360604 >500 0.0016942070225991705 0.5969568515544567 >1k 0.0010588793891244815 1.0106444361707025 >5k 4.235517556497926E-4 1.4229719520156072 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7356 0.8311817447359056 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5199 0.5874543081677505 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3937 0.44485624375003535 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1567 0.17706114654719465 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1206 0.13627041655131894 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1180 0.13333258004192067 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1027 0.11604454212123096 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 938 0.10598810176213694 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.1299371189993277E-4 0.0 14-15 0.0 0.0 0.0 1.6949056784989916E-4 0.0 16-17 0.0 0.0 0.0 2.824842797498319E-4 0.0 18-19 0.0 0.0 0.0 3.389811356997983E-4 0.0 20-21 0.0 0.0 0.0 3.389811356997983E-4 0.0 22-23 0.0 0.0 0.0 6.779622713995966E-4 0.0 24-25 0.0 0.0 0.0 6.779622713995966E-4 0.0 26-27 0.0 0.0 0.0 8.474528392494957E-4 0.0 28-29 0.0 0.0 0.0 0.0010734402630493613 0.0 30-31 0.0 0.0 0.0 0.002542358517748487 0.0 32-33 0.0 0.0 0.0 0.003672295636747815 0.0 34-35 0.0 0.0 0.0 0.004802232755747142 0.0 36-37 0.0 0.0 0.0 0.006271151010446269 0.0 38-39 0.0 0.0 0.0 0.008474528392494959 0.0 40-41 0.0 0.0 0.0 0.013220264292292134 0.0 42-43 0.0 0.0 0.0 0.0193219247348885 0.0 44-45 0.0 0.0 0.0 0.030621295924881782 0.0 46-47 0.0 0.0 0.0 0.05841774905226524 0.0 48-49 0.0 0.0 0.0 0.08028203230490223 0.0 50-51 0.0 0.0 0.0 0.11158129050118362 0.0 52-53 0.0 0.0 0.0 0.15446240416720808 0.0 54-55 0.0 0.0 0.0 0.20434912797102842 0.0 56-57 0.0 0.0 0.0 0.26169343676024426 0.0 58-59 0.0 0.0 0.0 0.3360432991904001 0.0 60-61 0.0 0.0 0.0 0.4304495454827939 0.0 62-63 0.0 0.0 0.0 0.5406749114411783 0.0 64-65 0.0 0.0 0.0 0.6344596923181225 0.0 66-67 0.0 0.0 0.0 0.7719730397003407 0.0 68-69 0.0 0.0 0.0 0.9542883938508822 0.0 70-71 0.0 0.0 0.0 1.0933836531996994 0.0 72-73 0.0 0.0 0.0 1.339540454573703 0.0 74-75 0.0 0.0 0.0 1.5516296518098769 0.0 76-77 0.0 0.0 0.0 1.7356399116389172 0.0 78-79 0.0 0.0 0.0 1.9806102790379716 0.0 80-81 0.0 0.0 0.0 2.2333207157021713 0.0 82-83 0.0 0.0 0.0 2.5250139829718474 0.0 84-85 0.0 0.0 0.0 2.9074976977531204 0.0 86-87 0.0 0.0 0.0 3.417042841565867 0.0 88-89 0.0 0.0 0.0 4.016304992627161 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 7725 0.0 34.452164 1 GGTATCA 4915 0.0 32.102695 1 CGTATAG 75 3.0681887E-4 31.683687 1 GTACATG 6335 0.0 29.258022 1 TACATGG 6445 0.0 28.4508 2 TATCAAC 9435 0.0 27.792479 2 ATCAACG 9430 0.0 27.605715 3 TCAACGC 9480 0.0 27.460115 4 CAACGCA 9545 0.0 27.27775 5 ACATGGG 6570 0.0 26.969542 3 AACGCAG 9705 0.0 26.86786 6 ATGGGAT 1735 0.0 25.74141 5 GAGTACT 5265 0.0 24.629606 12-13 TGCACCG 155 1.406901E-6 24.522358 5 TACCTGG 570 0.0 24.1687 2 ACGCAGA 10735 0.0 24.071457 7 CGCAGAG 10805 0.0 23.871548 8 CATGGGA 4740 0.0 23.551558 4 GCAGAGT 11015 0.0 23.157694 9 GTACTTT 5610 0.0 23.070004 14-15 >>END_MODULE