FastQCFastQC Report
Thu 26 May 2016
SRR937367_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937367_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885005
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75700.855362399082491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57390.6484709125937141No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44750.5056468607521991No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT14510.16395387596680244No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA13570.15333246704820877No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12990.14677883175801268No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12200.13785232851791798No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA96800.028.3734021
GGTATCA66850.025.3761521
GCACCGT1351.3424291E-524.6292636
AGTACTT60550.023.73137712-13
TATCAAC115900.023.6485252
GAGTACT59250.023.61068712-13
ATCAACG115850.023.494723
CAACGCA117050.023.2944345
TCAACGC117050.023.253854
GTACATG70350.023.0329321
AACGCAG118450.023.0178076
GTACTTT62250.022.47155414-15
TACATGG72400.022.3075582
GTATAGG3059.094947E-1221.8115791
ACATGGG70500.021.3590453
TACCTGG6200.020.6863462
ACGCAGA131650.020.6389147
CGCAGAG131600.020.610668
TACTTTT69100.020.58760814-15
CCTAGAC2108.5996544E-720.357993