FastQCFastQC Report
Thu 26 May 2016
SRR937366_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937366_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856854
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75220.8778625063313003No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52110.6081549482175493No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39210.4576042126196528No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN12650.1476330856832086No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA12210.14249802183335783No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12080.1409808438777201No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT10190.11892341052267946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9400.10970363679226565No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCGG651.3164025E-436.5925031
GTATCAA72650.030.644021
GGTATCA48850.027.9481331
GTATTAG2355.456968E-1226.3154621
GTACATG61200.025.8060871
CGGGTCG1501.0594431E-625.333716
TACATGG63400.024.9003982
TATCAAC89400.024.8923452
CAACGGT1151.2721182E-424.8090924
ATCAACG89200.024.7882353
TCAACGC90200.024.4607034
CAACGCA91550.024.0985935
AACGCAG93800.023.598436
ACATGGG63350.023.5689643
GAGTACT49200.022.34912312-13
AGTACTT51500.022.27329812-13
CATGGGG15600.021.9465074
CCAACGG1302.9194917E-421.9465053
CCTAGAC1553.8728773E-521.4745393
ACGGTGT1553.9044688E-521.4519316
Produced by FastQC (version 0.11.5)