FastQCFastQC Report
Thu 26 May 2016
SRR937366_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937366_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences856854
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT76070.8877825160412393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56300.6570547607877189No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT45180.5272776925824002No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14050.16397192520546092No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13680.1596538033317228No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13160.15358509150917193No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12430.1450655537582832No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATCACG550.002580107734.5431442
GTATCAA91950.028.5335661
CGTATAG1503.3140168E-828.5180631
CGTAAGA700.00839314127.1410392
TAGGGCG751.0612384E-625.33163630-31
AGTACTT58050.024.6732112-13
TAAGGTG2900.024.567325
GTACATG67050.024.2435441
GGTATCA63700.023.6531261
TACATGG69550.023.4240252
ATCAACG112000.023.366743
TATCAAC113200.023.1609942
TCAACGC113000.023.1599544
GAGTACT57200.023.12968412-13
CAACGCA114300.022.8549885
ACATGGG69400.022.5849443
AACGCAG116500.022.504936
GTACTTT60900.021.40866514-15
CATGGGG18800.021.2219834
TGAACCG1805.169213E-621.1096975
Produced by FastQC (version 0.11.5)