##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937366_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 856854 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.279555210105805 33.0 31.0 34.0 31.0 34.0 2 32.4910019676631 34.0 31.0 34.0 31.0 34.0 3 32.558465036050485 34.0 31.0 34.0 31.0 34.0 4 36.16074383733985 37.0 35.0 37.0 35.0 37.0 5 36.03480406230233 37.0 35.0 37.0 35.0 37.0 6 36.134853779056876 37.0 35.0 37.0 35.0 37.0 7 36.09885231322956 37.0 35.0 37.0 35.0 37.0 8 36.08217269219727 37.0 35.0 37.0 35.0 37.0 9 37.78871896495786 39.0 37.0 39.0 35.0 39.0 10-11 37.71796362040674 39.0 37.0 39.0 35.0 39.0 12-13 37.63608152614098 39.0 37.0 39.0 35.0 39.0 14-15 38.824154990231705 40.0 38.0 41.0 35.0 41.0 16-17 38.818832613257335 40.0 38.0 41.0 35.0 41.0 18-19 38.872604901185035 40.0 38.0 41.0 35.0 41.0 20-21 38.807531388077784 40.0 38.0 41.0 35.0 41.0 22-23 38.6773563524241 40.0 38.0 41.0 34.5 41.0 24-25 38.53647354158352 40.0 38.0 41.0 34.0 41.0 26-27 38.30511032217858 40.0 38.0 41.0 34.0 41.0 28-29 38.266205794686144 40.0 38.0 41.0 34.0 41.0 30-31 38.33226255581464 40.0 38.0 41.0 34.0 41.0 32-33 38.24496471977723 40.0 37.0 41.0 34.0 41.0 34-35 38.11332035562651 40.0 37.0 41.0 34.0 41.0 36-37 37.93994601180598 40.0 37.0 41.0 33.5 41.0 38-39 37.788482635314764 40.0 37.0 41.0 33.0 41.0 40-41 37.60298020432886 40.0 37.0 41.0 33.0 41.0 42-43 37.34604203283173 40.0 36.5 41.0 33.0 41.0 44-45 36.9636390797032 40.0 36.0 41.0 31.5 41.0 46-47 36.501848039455965 40.0 36.0 41.0 30.5 41.0 48-49 36.08950532996286 39.5 35.5 41.0 30.0 41.0 50-51 35.85190650916026 39.0 35.0 41.0 29.0 41.0 52-53 35.61246315008158 39.0 35.0 41.0 28.0 41.0 54-55 35.403765402273905 39.0 35.0 40.5 28.0 41.0 56-57 35.16509405336265 38.5 35.0 40.0 27.0 41.0 58-59 34.9109404869441 38.0 35.0 40.0 26.5 41.0 60-61 34.5766688373982 38.0 34.0 40.0 26.0 41.0 62-63 34.212364066690476 37.5 34.0 40.0 25.5 41.0 64-65 33.86935288859012 37.0 34.0 40.0 25.0 41.0 66-67 33.47722599182591 36.0 33.0 39.0 23.5 41.0 68-69 33.0359471975389 36.0 33.0 39.0 22.0 40.0 70-71 32.653150361671884 35.0 33.0 38.5 21.5 40.0 72-73 32.22350248700479 35.0 32.0 37.5 20.0 40.0 74-75 31.792863778426664 35.0 32.0 37.0 19.0 39.0 76-77 30.47037651688619 34.0 30.0 35.5 18.0 38.5 78-79 31.069309940783377 34.5 31.0 36.0 17.5 38.5 80-81 30.895870825134736 35.0 31.0 36.0 14.5 37.5 82-83 30.579169263375093 34.5 31.0 35.0 9.5 37.0 84-85 30.25693116913733 34.0 31.0 35.0 7.0 36.5 86-87 29.939120900410103 34.0 31.0 35.0 2.0 36.0 88-89 29.59516323667743 34.0 30.5 35.0 2.0 36.0 90-91 29.182881214302554 34.0 30.0 35.0 2.0 35.5 92-93 28.79728226745747 34.0 29.0 35.0 2.0 35.0 94-95 28.420359827928678 34.0 29.0 35.0 2.0 35.0 96-97 28.042794338358693 34.0 29.0 35.0 2.0 35.0 98-99 27.460389401228213 33.5 28.0 35.0 2.0 35.0 100-101 25.961750776678407 32.0 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.001560119424645734 1101 2 0.052683409098726486 1101 3 0.01651979383238711 1101 4 0.07130507725063495 1101 5 -0.09943158550917985 1101 6 -0.009652393035679552 1101 7 -0.001888583761754603 1101 8 -0.0736894203787486 1101 9 -0.0822201926870818 1101 10-11 0.007521577589166384 1101 12-13 0.11217092979217824 1101 14-15 0.16050669978339016 1101 16-17 0.19816452024643638 1101 18-19 0.14144258196765946 1101 20-21 0.010933457002273883 1101 22-23 0.03823688168565553 1101 24-25 0.0026240976267359883 1101 26-27 -0.06216969104425374 1101 28-29 -0.01478556683435528 1101 30-31 0.014397230003226014 1101 32-33 0.0641761358814037 1101 34-35 0.11518862470323654 1101 36-37 0.175163143928053 1101 38-39 0.2057287649669135 1101 40-41 0.05751568605166568 1101 42-43 0.024327342642266103 1101 44-45 0.09092175357969978 1101 46-47 0.20906241170103357 1101 48-49 0.2880967320928889 1101 50-51 0.26602551474989866 1101 52-53 0.4816612606838291 1101 54-55 0.3954902284179198 1101 56-57 0.2893441743537011 1101 58-59 0.24526566545926443 1101 60-61 0.26411349253424987 1101 62-63 0.29996123380851714 1101 64-65 0.37686951315208006 1101 66-67 0.45897422209467464 1101 68-69 0.35519136697833176 1101 70-71 0.26272820449717216 1101 72-73 0.2696790868011547 1101 74-75 0.2709091333254712 1101 76-77 0.3702466398085882 1101 78-79 0.2643930890212438 1101 80-81 0.36411034457652747 1101 82-83 0.32513962099892524 1101 84-85 0.34606878476035874 1101 86-87 0.15761918115765639 1101 88-89 0.1683702191934593 1101 90-91 0.4167342872074826 1101 92-93 0.47678154925553784 1101 94-95 0.34981685613121627 1101 96-97 0.42579827781217716 1101 98-99 0.48809809881214505 1101 100-101 0.5590479405052271 1102 1 0.1464802614531635 1102 2 0.12424760882188224 1102 3 0.10664829115477659 1102 4 0.044938657138494875 1102 5 -0.08139022587048572 1102 6 0.040737013975039815 1102 7 0.12350187192134854 1102 8 0.0694389790675558 1102 9 0.08541637941264213 1102 10-11 0.08508138687061972 1102 12-13 0.17789472346938595 1102 14-15 0.2955715077365255 1102 16-17 0.20265633361071878 1102 18-19 0.1280097878849702 1102 20-21 0.1962107958912469 1102 22-23 0.1043256133513637 1102 24-25 0.12746295163756116 1102 26-27 0.17710308961543575 1102 28-29 0.028690292727439726 1102 30-31 0.10112732969701455 1102 32-33 0.23171762835193732 1102 34-35 0.1921178323263888 1102 36-37 0.2442706538205286 1102 38-39 0.26474594921165817 1102 40-41 0.3292113193798727 1102 42-43 0.5425931181704442 1102 44-45 0.5730516770965224 1102 46-47 0.7319556288880733 1102 48-49 0.8366943075623468 1102 50-51 0.7340542903200173 1102 52-53 0.6269995623938627 1102 54-55 0.5579121023255524 1102 56-57 0.6052881892308619 1102 58-59 0.5900915159325635 1102 60-61 0.6897717092319127 1102 62-63 0.6185451066255325 1102 64-65 0.8584681093293298 1102 66-67 0.8106115492385157 1102 68-69 0.6584911894031364 1102 70-71 0.7188000175818061 1102 72-73 0.654767664661577 1102 74-75 0.5402361653029857 1102 76-77 0.504882890315443 1102 78-79 0.6689750882102707 1102 80-81 0.6051462930657401 1102 82-83 0.5617578623054307 1102 84-85 0.6971083686471609 1102 86-87 0.7242462313121614 1102 88-89 0.6713099974342036 1102 90-91 0.5052904750736431 1102 92-93 0.31205469345498216 1102 94-95 0.301852678779742 1102 96-97 0.5132344835756797 1102 98-99 0.6556948213613971 1102 100-101 0.6725555398844101 1103 1 -0.001181968164139846 1103 2 -0.02396925196569555 1103 3 -0.030609057708232967 1103 4 -0.06723133731519226 1103 5 -0.06145539503298636 1103 6 0.011019875871880913 1103 7 0.013055393829283446 1103 8 0.012171924159069647 1103 9 0.14604311263504854 1103 10-11 0.04351247394770752 1103 12-13 0.12675426312551252 1103 14-15 0.06324829642204577 1103 16-17 -0.08940550776476641 1103 18-19 0.014674374684751967 1103 20-21 -0.05711976428623444 1103 22-23 -0.046875163132277464 1103 24-25 -0.11976735895589741 1103 26-27 -0.08876282829914572 1103 28-29 -0.1445369673945791 1103 30-31 -0.04855445066904451 1103 32-33 0.047082298346388995 1103 34-35 0.17426425495533238 1103 36-37 0.10909871815774608 1103 38-39 0.021033386815652477 1103 40-41 0.1019449577603524 1103 42-43 -0.15563414195158032 1103 44-45 -0.04739757415139678 1103 46-47 -0.061133666730334824 1103 48-49 0.11137054161670079 1103 50-51 0.18315786769106523 1103 52-53 0.09471448197233912 1103 54-55 0.09099793149915314 1103 56-57 0.283069664549771 1103 58-59 0.23698835453489409 1103 60-61 0.20546153174993975 1103 62-63 0.2685291609793836 1103 64-65 0.10324556357225134 1103 66-67 0.19464649100223852 1103 68-69 0.22989024653015377 1103 70-71 0.0925881484747606 1103 72-73 0.42938496915409274 1103 74-75 0.11808350307337179 1103 76-77 -0.04327226775443904 1103 78-79 0.2604400077887483 1103 80-81 0.11079451824599573 1103 82-83 0.19348345853394022 1103 84-85 0.016033770754756205 1103 86-87 0.2893453716338463 1103 88-89 0.4390880063083067 1103 90-91 0.22044577180131952 1103 92-93 0.2333081598997957 1103 94-95 0.18790509142533196 1103 96-97 0.21065822178976745 1103 98-99 0.11236980749561809 1103 100-101 0.2020591449870217 1104 1 -0.05569297060912959 1104 2 -0.058512854247680934 1104 3 -0.0263099542037466 1104 4 0.09306409870762877 1104 5 -0.2044729174453792 1104 6 -0.07600538900179288 1104 7 -0.06052651326118763 1104 8 -0.06217811659072936 1104 9 -0.06527718076348776 1104 10-11 0.031930363108266135 1104 12-13 -0.0938192902567252 1104 14-15 -0.08271034253476017 1104 16-17 -0.2230342771209166 1104 18-19 -0.11850105318811188 1104 20-21 -0.11922652875241369 1104 22-23 -0.11938127560171097 1104 24-25 -0.12726022774155155 1104 26-27 -0.22021657728040367 1104 28-29 -0.29554858108651416 1104 30-31 -0.2538223805793933 1104 32-33 -0.2590892582958233 1104 34-35 -0.13933802703325426 1104 36-37 -0.022779847449434953 1104 38-39 -0.1061273711305617 1104 40-41 -0.05811922316874529 1104 42-43 0.1482645948047434 1104 44-45 0.25193412919008296 1104 46-47 0.11650284508710484 1104 48-49 -0.15364270206708142 1104 50-51 -0.03249311030403845 1104 52-53 0.12274219183379387 1104 54-55 0.14447490786426442 1104 56-57 0.04887243553591958 1104 58-59 0.0014993569798846806 1104 60-61 0.010800773803730124 1104 62-63 0.07045153261752546 1104 64-65 -0.10315521067387579 1104 66-67 -0.060435008590260964 1104 68-69 5.97255495108584E-4 1104 70-71 0.030681058010209483 1104 72-73 0.06123908488351759 1104 74-75 -0.08911189219965365 1104 76-77 -0.1303433761488968 1104 78-79 -0.0023564469789754128 1104 80-81 0.1408240618482779 1104 82-83 0.13275709341576558 1104 84-85 -0.04036598474646169 1104 86-87 0.026212731047049687 1104 88-89 0.27426832415671143 1104 90-91 0.11952890138567085 1104 92-93 0.05121362037249355 1104 94-95 -0.1824881441084898 1104 96-97 -0.19121219385815635 1104 98-99 0.1653730674793188 1104 100-101 0.4954536030147274 1105 1 -0.045566972376441583 1105 2 -0.023563467073813626 1105 3 0.013711357905357602 1105 4 0.03480390559410296 1105 5 -0.07682958767550474 1105 6 0.03192973265367982 1105 7 0.0051208248879746066 1105 8 0.033294502086612 1105 9 -0.05262860879151532 1105 10-11 -0.09551694194670546 1105 12-13 -0.01777852260882895 1105 14-15 0.09008801841024905 1105 16-17 0.07013184129449002 1105 18-19 0.015946840284293273 1105 20-21 -0.11000238152858088 1105 22-23 -0.13729779722382318 1105 24-25 -0.13544589026732723 1105 26-27 -0.19298390383972475 1105 28-29 -0.06759895840833252 1105 30-31 -0.1266620749318932 1105 32-33 -0.07491082156374063 1105 34-35 0.012099132798638834 1105 36-37 -0.05084090554345977 1105 38-39 -0.054683757245008735 1105 40-41 -0.03401298944549325 1105 42-43 -0.2428283991826916 1105 44-45 0.2000160737003327 1105 46-47 0.12007385924550817 1105 48-49 0.0077862254133904685 1105 50-51 -0.04654200593993352 1105 52-53 0.08566365355336103 1105 54-55 0.058141641131271626 1105 56-57 0.22873661203643536 1105 58-59 0.18303300491400165 1105 60-61 0.22385629445705035 1105 62-63 0.3048184757785961 1105 64-65 0.281591182780339 1105 66-67 0.13164312116230548 1105 68-69 0.099806702581823 1105 70-71 0.09208828888476006 1105 72-73 0.199043772860918 1105 74-75 0.3520525426689858 1105 76-77 0.24738801025735668 1105 78-79 0.3989112939443267 1105 80-81 0.5523527181898338 1105 82-83 0.5440917848467741 1105 84-85 0.4395924621335858 1105 86-87 0.3964385336669842 1105 88-89 0.34738588611734755 1105 90-91 0.2431992118462496 1105 92-93 0.28427979708036233 1105 94-95 0.4168246364969441 1105 96-97 0.2599182611045663 1105 98-99 0.05466270274975926 1105 100-101 -0.1464988443418207 1106 1 -0.12290907605171952 1106 2 -0.008645891433815223 1106 3 -0.1599759359191708 1106 4 -0.04517709131942382 1106 5 -0.08761964405081812 1106 6 0.043277355472966406 1106 7 -0.05421417401598916 1106 8 -0.03768981654809522 1106 9 -0.2539055123271794 1106 10-11 -0.07805862003838371 1106 12-13 -0.19086624155868037 1106 14-15 -0.1128589111935483 1106 16-17 -0.06635851471550325 1106 18-19 -0.07201155762108868 1106 20-21 -0.20527177395683083 1106 22-23 -0.1693154774266219 1106 24-25 -0.028910982407090557 1106 26-27 0.10871186193958948 1106 28-29 0.09990584010012782 1106 30-31 0.015136619140569962 1106 32-33 -0.007256625799875849 1106 34-35 0.10961330300610683 1106 36-37 0.14457620380295566 1106 38-39 0.07773216288092755 1106 40-41 0.13485784685071422 1106 42-43 -0.009491425118696384 1106 44-45 0.2798191076255776 1106 46-47 0.31037434173142486 1106 48-49 0.2172639384133177 1106 50-51 0.30318619951337666 1106 52-53 0.4667694834833682 1106 54-55 0.4883728937233869 1106 56-57 0.38699229978701055 1106 58-59 0.2532122892583786 1106 60-61 0.3785527224419951 1106 62-63 0.47149418062278414 1106 64-65 0.4722766152494984 1106 66-67 0.5104533813014172 1106 68-69 0.40133252516417883 1106 70-71 0.3865379822614017 1106 72-73 0.37985187758564365 1106 74-75 0.407536132266479 1106 76-77 0.3551400169237091 1106 78-79 0.46885380349742434 1106 80-81 0.4483054732172853 1106 82-83 0.5309332167690535 1106 84-85 0.4497546834171935 1106 86-87 0.6294039240666045 1106 88-89 0.5625282450511264 1106 90-91 0.5748504257753311 1106 92-93 0.5787871565755296 1106 94-95 0.5548112564298684 1106 96-97 0.8305237400732395 1106 98-99 0.9671129323067404 1106 100-101 0.8849437022550752 1107 1 -0.023454695436868178 1107 2 0.09981862682218434 1107 3 0.05742124532206816 1107 4 0.08238987480601878 1107 5 0.02771127163367737 1107 6 0.06101987587187807 1107 7 -0.012247636473752266 1107 8 0.009671924159064815 1107 9 0.055740082332015106 1107 10-11 0.01824732243255056 1107 12-13 0.03989820251945275 1107 14-15 0.10586193278568601 1107 16-17 0.09339752253826816 1107 18-19 0.005166798927170646 1107 20-21 -0.017725824892302455 1107 22-23 0.005700594443482032 1107 24-25 0.09883112589258758 1107 26-27 0.02710838382206049 1107 28-29 0.09550091139330164 1107 30-31 0.11474100387641073 1107 32-33 0.12700654077062978 1107 34-35 0.1547188004098814 1107 36-37 0.12106841512743927 1107 38-39 0.20001065954291875 1107 40-41 0.3385737456391382 1107 42-43 0.4207673731999364 1107 44-45 0.3885115167576956 1107 46-47 0.5564420908454153 1107 48-49 0.6441356931318509 1107 50-51 0.6855442313274338 1107 52-53 0.6539569062147663 1107 54-55 0.5727782345294514 1107 56-57 0.6677666342467461 1107 58-59 0.7060792636258029 1107 60-61 0.5581509256893327 1107 62-63 0.8158397670399893 1107 64-65 0.6179046544813431 1107 66-67 0.6305555819113309 1107 68-69 0.5804205495604577 1107 70-71 0.4795578454444538 1107 72-73 0.5757486055177239 1107 74-75 0.6150153212551857 1107 76-77 0.5438868231546508 1107 78-79 0.6532051593039014 1107 80-81 0.7662111849126596 1107 82-83 0.6036728524733341 1107 84-85 0.6305034677244556 1107 86-87 0.7833984019368749 1107 88-89 0.7940501275204248 1107 90-91 0.6157488021043491 1107 92-93 0.5622475538391889 1107 94-95 0.46722327324351554 1107 96-97 0.6308097369412806 1107 98-99 0.7029758681016816 1107 100-101 1.1299379328658077 1108 1 -0.1045540611874003 1108 2 -0.02144987212073346 1108 3 -0.04033773987877254 1108 4 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-1.1895654078875602 2316 90-91 -1.376297265036058 2316 92-93 -1.407006305509011 2316 94-95 -1.093745851970894 2316 96-97 -1.3318440243917244 2316 98-99 -1.0487577756130975 2316 100-101 -1.4561573869087603 >>END_MODULE >>Per sequence quality scores pass #Quality Count 4 7.0 5 15.0 6 30.0 7 84.0 8 182.0 9 374.0 10 653.0 11 1260.0 12 2055.0 13 2964.0 14 3566.0 15 5368.0 16 8028.0 17 11924.0 18 10259.0 19 5059.0 20 4049.0 21 4015.0 22 4130.0 23 4377.0 24 5046.0 25 5918.0 26 6998.0 27 8674.0 28 10642.0 29 13390.0 30 16842.0 31 21339.0 32 27389.0 33 36863.0 34 51762.0 35 77391.0 36 120782.0 37 192372.0 38 173020.0 39 20027.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.947112471233304 20.111061942252928 15.335935520556152 20.605890065957624 2 15.523414724095353 21.738359160370376 39.94531156999909 22.79291454553518 3 19.297453241742467 26.991529478767678 30.168500117873055 23.5425171616168 4 12.591337350367802 17.424733121627913 37.48446345980806 32.49946606819622 5 12.878284731811343 39.24776679673502 33.26057598143637 14.613372490017262 6 30.47391971094259 37.45457219082831 17.329673433280348 14.741834664948755 7 27.90078589818102 34.46188032033462 20.362395460603558 17.274938320880803 8 27.38296139132221 34.30199310500972 19.291034411930152 19.024011091737915 9 27.896701188300455 16.368366139388975 20.532436097631567 35.202496574678996 10-11 24.936220172864925 27.798551445170354 26.528206672315235 20.737021709649486 12-13 26.456670029989795 25.345314466821854 27.692268188935117 20.505747314253234 14-15 23.38368030026119 25.914274777266606 27.254701501072525 23.447343421399676 16-17 21.154654118437914 29.5510670429268 27.63527975594442 21.658999082690865 18-19 21.112173135680056 27.204984746526247 31.53226804099648 20.150574076797216 20-21 22.232142231932162 26.05624762211532 31.63549449497814 20.076115650974376 22-23 21.764280929272843 26.259275338977318 31.47488101657015 20.501562715179695 24-25 21.637263443272776 26.517052858112354 31.562798877983965 20.282884820630905 26-27 21.378919972456615 26.57761749705309 31.762134845885413 20.28132768460488 28-29 21.025373791036483 26.7025275755139 31.88664249225925 20.385456141190367 30-31 21.18870362179214 26.66702457048624 31.669411244326547 20.474860563395076 32-33 21.027502935155816 26.775565032082476 31.557184771267917 20.639747261493792 34-35 21.470985722188377 26.52126266551828 31.4918877661772 20.51586384611614 36-37 21.520990559018824 26.42284321809139 31.39291089041891 20.66325533247087 38-39 21.39232152204092 26.796654488331967 31.375171485774338 20.43585250385278 40-41 21.303654185723804 26.870361660634906 31.311963662378496 20.514020491262798 42-43 21.26612936171206 27.04019282283527 30.7968532060298 20.896824609422872 44-45 21.418993200708638 27.034652344506764 30.543243072915573 21.003111381869022 46-47 21.72377091079694 27.542031664670997 29.906436802535787 20.82776062199628 48-49 21.6846666635545 27.680626292521932 29.432854215197025 21.201852828726548 50-51 21.77195024814656 27.864655818825295 29.224749884023215 21.138644049004935 52-53 21.956993639493493 28.1773355896598 28.684892338215555 21.180778432631147 54-55 21.559403095161542 28.129837831087812 28.755090572659153 21.555668501091493 56-57 21.733749273505172 28.362533173679534 28.465993039654364 21.437724513160937 58-59 21.91009203434891 27.704136293931054 28.948047158559103 21.437724513160937 60-61 21.84333620430085 27.94110782000201 29.139853463950683 21.075702511746456 62-63 21.868311287570577 27.90323672410936 29.262161348374406 20.96629063994566 64-65 21.704806186351465 27.95388712662834 29.066737157088607 21.274569529931586 66-67 21.778972847182835 28.11488304892082 28.681257250360037 21.42488685353631 68-69 21.816318766090838 28.10087832933032 28.807474785669434 21.275328118909407 70-71 21.755281529875568 28.32279478184148 28.672445947617682 21.24947774066527 72-73 21.895270372782292 28.03272202732321 28.460857975804515 21.611149624089983 74-75 21.670319564359854 28.683533017293495 28.19751089450478 21.44863652384187 76-77 21.600120907412464 28.291517574756025 28.676180539508483 21.43218097832303 78-79 21.609107269149703 28.100586564338847 28.580131504316952 21.710174662194493 80-81 21.58634959981514 28.646595569373545 28.32909690565721 21.437957925154112 82-83 21.581690589590465 28.421577197382504 28.248384487815237 21.748347725211794 84-85 21.556485443544315 28.262357567542175 28.101594963433072 22.079562025480435 86-87 21.410648721952633 28.112665634985657 28.264150018556254 22.21253562450546 88-89 21.47769631699216 27.893725185387474 28.37111106442871 22.257467433191653 90-91 21.353206806359108 27.779191327599506 28.6187232065846 22.248878659456782 92-93 21.30715719976915 27.82199404437136 28.718781237727388 22.152067518132107 94-95 21.058254965256626 27.737630914951673 28.602072231675407 22.602041888116293 96-97 21.139248926888364 27.65774566028752 28.485424588086182 22.717580824737936 98-99 21.101249350816076 27.799426967537887 28.21519644743215 22.88412723421389 100-101 20.8699275575883 27.951285644170206 28.393305160960118 22.785481637281375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 885.0 1 622.0 2 469.5 3 592.5 4 521.0 5 505.5 6 887.5 7 1848.0 8 2744.5 9 2965.5 10 2594.5 11 2143.5 12 2066.5 13 2114.0 14 2198.5 15 2255.5 16 2267.5 17 2327.5 18 2358.0 19 2397.0 20 2553.0 21 2812.0 22 3041.5 23 3447.5 24 3957.5 25 4560.0 26 5566.0 27 6628.0 28 7418.0 29 8721.0 30 10491.0 31 11779.5 32 13625.0 33 16057.0 34 18647.5 35 20504.0 36 22950.0 37 25387.5 38 26421.0 39 27063.0 40 26820.5 41 27004.5 42 27202.0 43 28043.0 44 29483.5 45 30710.0 46 30562.5 47 30462.0 48 34035.5 49 33537.0 50 29962.5 51 27737.5 52 25069.5 53 24221.5 54 23822.5 55 22572.5 56 20698.5 57 18751.0 58 17233.0 59 15664.5 60 13418.5 61 11145.5 62 8943.0 63 6528.5 64 4566.5 65 3289.0 66 2439.0 67 1980.0 68 1486.0 69 994.0 70 703.5 71 503.0 72 381.5 73 301.5 74 210.0 75 150.0 76 100.5 77 63.0 78 40.0 79 18.5 80 17.5 81 12.5 82 5.5 83 3.5 84 1.5 85 1.0 86 1.5 87 1.0 88 1.0 89 1.5 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04609886865206908 2 0.0 3 0.0 4 3.5011798976254996E-4 5 0.014588249573439582 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.007294124786719791 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0014004719590501999 24-25 4.0847098805630834E-4 26-27 0.0028009439181003997 28-29 0.002917649914687916 30-31 0.0015171779556377165 32-33 0.0 34-35 0.0 36-37 5.251769846438249E-4 38-39 5.251769846438249E-4 40-41 1.1670599658751667E-4 42-43 0.012720953628039315 44-45 0.0 46-47 0.0 48-49 2.3341199317503334E-4 50-51 1.7505899488127498E-4 52-53 4.6682398635006667E-4 54-55 5.8352998293758334E-5 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 5.835299829375833E-4 84-85 5.8352998293758334E-5 86-87 0.0 88-89 0.0 90-91 0.007819301771363616 92-93 0.0020423549402815416 94-95 0.0 96-97 0.0 98-99 0.0010503539692876499 100-101 0.012195776643395492 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 856854.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.63725568793015 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96788265942564 38.71135725267507 2 13.614008710772389 14.059800974717243 3 4.693867409190502 7.271352947208366 4 2.1284904700083795 4.396376265165812 5 1.1523557860481433 2.975224518381685 6 0.712951808557561 2.2088924898995406 7 0.49134554340791614 1.7760214819255684 8 0.33670644462292915 1.3909277418214472 9 0.2770188052867018 1.2874041791058923 >10 1.4576417424397317 13.856453664420759 >50 0.10985427253655718 3.904563877422504 >100 0.05447185666721798 4.851806252593183 >500 0.001815728552702882 0.6040439007093532 >1k 0.0011348303454393012 1.1544106367844644 >5k 4.539321381757205E-4 1.5513638171691324 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7607 0.8877825160412393 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5630 0.6570547607877189 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4518 0.5272776925824002 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1405 0.16397192520546092 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1368 0.1596538033317228 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1316 0.15358509150917193 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1243 0.1450655537582832 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.1670599658751665E-4 0.0 10-11 0.0 0.0 0.0 1.1670599658751665E-4 0.0 12-13 0.0 0.0 0.0 2.334119931750333E-4 0.0 14-15 0.0 0.0 0.0 2.334119931750333E-4 0.0 16-17 0.0 0.0 0.0 2.9176499146879164E-4 0.0 18-19 0.0 0.0 0.0 4.084709880563083E-4 0.0 20-21 0.0 0.0 0.0 4.668239863500666E-4 0.0 22-23 0.0 0.0 0.0 9.336479727001332E-4 0.0 24-25 0.0 0.0 0.0 0.0011670599658751665 0.0 26-27 0.0 0.0 0.0 0.0015171779556377165 0.0 28-29 0.0 0.0 0.0 0.0019840019419877833 0.0 30-31 0.0 0.0 0.0 0.00315106190786295 0.0 32-33 0.0 0.0 0.0 0.004259768875444358 0.0 34-35 0.0 0.0 0.0 0.005893652827669592 0.0 36-37 0.0 0.0 0.0 0.007760948773069858 0.0 38-39 0.0 0.0 0.0 0.009861656711645158 0.0 40-41 0.0 0.0 0.0 0.013362836609270658 0.0 42-43 0.0 0.0 0.0 0.019139783440352734 0.0 44-45 0.0 0.0 0.0 0.028826381157116616 0.0 46-47 0.0 0.0 0.0 0.05257605146267626 0.0 48-49 1.1670599658751665E-4 0.0 0.0 0.07270783587402288 0.0 50-51 1.1670599658751665E-4 0.0 0.0 0.10112574604308319 0.0 52-53 1.1670599658751665E-4 0.0 0.0 0.14267308082823912 0.0 54-55 1.1670599658751665E-4 0.0 0.0 0.18171123668676342 0.0 56-57 1.1670599658751665E-4 0.0 0.0 0.22209151150604423 0.0 58-59 1.1670599658751665E-4 0.0 0.0 0.2856962796462408 0.0 60-61 1.1670599658751665E-4 0.0 0.0 0.37095001015342166 0.0 62-63 1.1670599658751665E-4 0.0 0.0 0.4755185830958366 0.0 64-65 1.1670599658751665E-4 0.0 0.0 0.5650320824784618 0.0 66-67 1.1670599658751665E-4 0.0 0.0 0.7035037474295505 0.0 68-69 1.1670599658751665E-4 0.0 0.0 0.8748281504200248 0.0 70-71 1.1670599658751665E-4 0.0 0.0 1.0063558085741562 0.0 72-73 1.1670599658751665E-4 0.0 0.0 1.2503880474386535 0.0 74-75 1.1670599658751665E-4 0.0 0.0 1.4455204737329814 0.0 76-77 1.1670599658751665E-4 0.0 0.0 1.621629822583544 0.0 78-79 1.1670599658751665E-4 0.0 0.0 1.8492065159292015 0.0 80-81 1.1670599658751665E-4 0.0 0.0 2.094930991744218 0.0 82-83 1.1670599658751665E-4 0.0 0.0 2.3926479890389727 0.0 84-85 1.1670599658751665E-4 0.0 0.0 2.788222964472361 0.0 86-87 1.1670599658751665E-4 0.0 0.0 3.312116183153723 0.0 88-89 1.1670599658751665E-4 0.0 0.0 3.9343925569583615 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TATCACG 55 0.0025801077 34.543144 2 GTATCAA 9195 0.0 28.533566 1 CGTATAG 150 3.3140168E-8 28.518063 1 CGTAAGA 70 0.008393141 27.141039 2 TAGGGCG 75 1.0612384E-6 25.331636 30-31 AGTACTT 5805 0.0 24.67321 12-13 TAAGGTG 290 0.0 24.56732 5 GTACATG 6705 0.0 24.243544 1 GGTATCA 6370 0.0 23.653126 1 TACATGG 6955 0.0 23.424025 2 ATCAACG 11200 0.0 23.36674 3 TATCAAC 11320 0.0 23.160994 2 TCAACGC 11300 0.0 23.159954 4 GAGTACT 5720 0.0 23.129684 12-13 CAACGCA 11430 0.0 22.854988 5 ACATGGG 6940 0.0 22.584944 3 AACGCAG 11650 0.0 22.50493 6 GTACTTT 6090 0.0 21.408665 14-15 CATGGGG 1880 0.0 21.221983 4 TGAACCG 180 5.169213E-6 21.109697 5 >>END_MODULE