FastQCFastQC Report
Thu 26 May 2016
SRR937365_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937365_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944661
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT79600.8426303192362128No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57680.6105894072053362No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44380.46979816039828043No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN15210.16101014014551251No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14540.1539176487650067No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT12280.12999372261583783No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT11440.12110164387012906No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.10755180959095378No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA56550.031.6227361
GTATCAA84600.031.425751
TATAACG650.005814993329.2600842
GTACATG70900.028.0397571
ACGACGG856.40456E-427.9424886
TACATGG72300.026.8976232
CGACGGT908.943896E-426.3901277
ACATGGG70200.025.601213
TATCAAC104450.025.4012242
ATCAACG103650.025.3665663
TCAACGC104400.025.1843344
CAACGCA104850.024.9388855
AACGCAG107750.024.3793356
GAGTACT55750.024.32496512-13
CATGGGA52700.023.7274444
ATGGGAT18850.023.7082375
GTCGGGA3650.023.4481472
ATGGGAG19150.023.336835
AGTACTT58700.022.65744212-13
TACCTGG5500.022.4770622
Produced by FastQC (version 0.11.5)