##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937365_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 944661 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.721521265300463 33.0 31.0 34.0 30.0 34.0 2 31.800437405587825 33.0 31.0 34.0 30.0 34.0 3 31.83429399541211 33.0 31.0 34.0 30.0 34.0 4 35.477578729300774 37.0 35.0 37.0 33.0 37.0 5 35.476465102295954 37.0 35.0 37.0 33.0 37.0 6 35.55420410073032 37.0 35.0 37.0 33.0 37.0 7 35.495132116177125 37.0 35.0 37.0 33.0 37.0 8 35.48929827737146 37.0 35.0 37.0 33.0 37.0 9 37.09329907765855 39.0 37.0 39.0 33.0 39.0 10-11 36.997494339239154 39.0 37.0 39.0 33.0 39.0 12-13 36.88899774628147 39.0 37.0 39.0 33.0 39.0 14-15 38.00743123723748 40.0 38.0 41.0 33.0 41.0 16-17 37.978775984189035 40.0 38.0 41.0 33.0 41.0 18-19 37.98605796153329 40.0 38.0 41.0 33.0 41.0 20-21 37.948719699447736 40.0 38.0 41.0 33.0 41.0 22-23 37.806352225824924 40.0 37.5 41.0 33.0 41.0 24-25 37.664724700183456 40.0 37.0 41.0 32.0 41.0 26-27 37.48511000242415 40.0 37.0 41.0 32.0 41.0 28-29 37.29687792763753 39.5 37.0 41.0 32.0 41.0 30-31 37.223210230971745 39.0 36.5 41.0 32.0 41.0 32-33 37.023465031371046 39.0 36.0 41.0 31.0 41.0 34-35 36.80648931203892 39.0 35.0 41.0 31.0 41.0 36-37 36.782640015836364 39.0 35.0 41.0 31.0 41.0 38-39 36.734402076512104 39.0 35.0 41.0 31.0 41.0 40-41 36.54040920499523 39.0 35.0 41.0 30.5 41.0 42-43 36.25740503736261 39.0 35.0 41.0 30.0 41.0 44-45 35.93488087260933 39.0 35.0 41.0 29.5 41.0 46-47 35.48095613135294 39.0 35.0 41.0 26.5 41.0 48-49 35.09581744138903 39.0 35.0 40.5 24.5 41.0 50-51 34.13655692359481 38.0 34.0 39.5 23.5 40.5 52-53 34.252242868076486 38.0 34.0 39.5 24.0 40.5 54-55 34.597040631506964 38.0 34.5 40.0 23.5 41.0 56-57 34.3863804052459 38.0 34.0 40.0 23.0 41.0 58-59 34.1467907535084 38.0 34.0 40.0 22.0 41.0 60-61 33.859716871978414 38.0 34.0 40.0 21.5 41.0 62-63 33.538882731477216 37.0 33.0 40.0 20.0 41.0 64-65 33.18389665710768 37.0 33.0 40.0 18.5 41.0 66-67 32.78809964632815 36.0 32.5 39.0 17.0 41.0 68-69 32.294495062249844 36.0 32.0 39.0 12.0 40.5 70-71 31.86291484458446 35.0 31.5 38.5 8.0 40.0 72-73 31.464895343408905 35.0 31.0 37.5 7.0 40.0 74-75 31.005982040118095 35.0 31.0 37.0 3.5 39.0 76-77 30.574583898350838 35.0 30.5 36.5 2.0 39.0 78-79 30.108323514996385 34.5 30.0 36.0 2.0 39.0 80-81 29.669657157435314 34.0 29.5 36.0 2.0 37.5 82-83 29.247435852649787 34.0 29.0 35.0 2.0 37.0 84-85 28.78585651360647 34.0 29.0 35.0 2.0 36.5 86-87 28.3691197159616 34.0 29.0 35.0 2.0 36.0 88-89 27.994750497797625 34.0 27.5 35.0 2.0 36.0 90-91 27.570598341627313 34.0 26.5 35.0 2.0 35.0 92-93 27.139428853313518 33.0 26.0 35.0 2.0 35.0 94-95 26.62652845835702 33.0 25.0 35.0 2.0 35.0 96-97 26.021938557853026 33.0 24.5 35.0 2.0 35.0 98-99 25.351064032494197 32.5 21.0 35.0 2.0 35.0 100-101 23.58586043035544 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.018775741865653117 1101 2 -0.19718362018677382 1101 3 0.1507923136067575 1101 4 -0.06992069260405032 1101 5 0.09015397420233029 1101 6 -0.05061645229200451 1101 7 -0.016297158047542837 1101 8 -0.08886584479420634 1101 9 -0.10259566482595517 1101 10-11 -0.08592204910204515 1101 12-13 -2.9230677158409435E-4 1101 14-15 0.023528642547631762 1101 16-17 0.08108004147518244 1101 18-19 0.19458567393230197 1101 20-21 0.04999581398895003 1101 22-23 -0.08115242394039512 1101 24-25 -0.0018827669516028323 1101 26-27 0.02526074297115599 1101 28-29 0.06052436900670699 1101 30-31 0.014548692896262594 1101 32-33 -0.06074053962638004 1101 34-35 -0.11090992088162466 1101 36-37 -0.025507513356174627 1101 38-39 -0.10297890526419451 1101 40-41 0.18509032162072003 1101 42-43 0.2997023975719486 1101 44-45 0.03764451700286742 1101 46-47 0.20651002941612262 1101 48-49 0.4759784676097354 1101 50-51 0.3328286341989539 1101 52-53 0.38825803460773045 1101 54-55 0.3509805394536514 1101 56-57 0.3276951561439816 1101 58-59 0.27056509530076767 1101 60-61 0.4479182378922175 1101 62-63 0.43895504099493365 1101 64-65 0.4826064245190196 1101 66-67 0.4776103765508566 1101 68-69 0.48074654964708685 1101 70-71 0.5075520978713257 1101 72-73 0.4618032702718615 1101 74-75 0.648597337380366 1101 76-77 0.7689885657696784 1101 78-79 0.7669643107620416 1101 80-81 0.8402093846370136 1101 82-83 0.9008238682113081 1101 84-85 0.8589678369334557 1101 86-87 1.052901044285317 1101 88-89 0.8083486115140275 1101 90-91 0.734021786945366 1101 92-93 0.6859138284877062 1101 94-95 0.5967243423252171 1101 96-97 0.6423490052975183 1101 98-99 0.9458396686547914 1101 100-101 1.0919285886479884 1102 1 -0.023320413100961446 1102 2 -0.12364092748688549 1102 3 -0.1876311810802207 1102 4 -0.07821188326256845 1102 5 -0.1180554577234858 1102 6 -0.1664670216878008 1102 7 -0.228917804006322 1102 8 -0.22559145289908145 1102 9 -0.00797256480949926 1102 10-11 -0.31166199154700536 1102 12-13 -0.057221197405354474 1102 14-15 -0.08617512133418614 1102 16-17 -0.011194276564992833 1102 18-19 -0.04348837019667684 1102 20-21 0.004367055646255835 1102 22-23 0.10752269587955254 1102 24-25 0.11858892003813537 1102 26-27 0.08864708343213579 1102 28-29 0.030797425034116088 1102 30-31 0.0811561460275172 1102 32-33 -0.05304148432198019 1102 34-35 0.2082095725511195 1102 36-37 0.2177412366162983 1102 38-39 0.2758762910798538 1102 40-41 0.22233297920710982 1102 42-43 0.08713779068114746 1102 44-45 0.1841947778922588 1102 46-47 0.3290360503313252 1102 48-49 0.39929629140217315 1102 50-51 0.3387564662450089 1102 52-53 0.4611088229769962 1102 54-55 0.4088387716251347 1102 56-57 0.33175491128240964 1102 58-59 0.3386250292459323 1102 60-61 0.5342203461272987 1102 62-63 0.6154534868015915 1102 64-65 0.5858261767032502 1102 66-67 0.44545302143937704 1102 68-69 0.3403447138979274 1102 70-71 0.313024944513451 1102 72-73 0.28662838303024074 1102 74-75 0.2831283519271963 1102 76-77 0.2500693877105604 1102 78-79 0.2292516376401892 1102 80-81 0.29608363944698013 1102 82-83 0.2539600476814421 1102 84-85 0.29053344540112036 1102 86-87 0.31090167399599977 1102 88-89 0.40235981673622234 1102 90-91 0.44470859917108285 1102 92-93 0.18029988000737163 1102 94-95 0.33326265685145273 1102 96-97 0.303658596205846 1102 98-99 0.7054521504016051 1102 100-101 0.509999147341297 1103 1 0.10240128382816849 1103 2 0.08330110072033392 1103 3 0.05171492996775129 1103 4 0.0310651771720174 1103 5 0.02721746641825007 1103 6 0.1525601751869985 1103 7 0.08308881006581714 1103 8 0.19408178995701064 1103 9 0.21675789551543545 1103 10-11 0.10927043526546498 1103 12-13 -0.012857520850459991 1103 14-15 -0.01988765647456603 1103 16-17 0.060371270313616776 1103 18-19 -0.05771062350215317 1103 20-21 0.005715222351845739 1103 22-23 0.024598579119199826 1103 24-25 0.007073865405850199 1103 26-27 -0.1056456566995223 1103 28-29 -0.1570145609734226 1103 30-31 -0.38250829471746073 1103 32-33 -0.14853090193144425 1103 34-35 -0.07496975297838304 1103 36-37 0.03408802330871907 1103 38-39 -0.12227311751269809 1103 40-41 -0.08230804877156572 1103 42-43 -0.23102736629690668 1103 44-45 -0.26951483486730154 1103 46-47 -0.14384762767226533 1103 48-49 -0.2416036770011445 1103 50-51 -0.2224755295787375 1103 52-53 -0.12825795704640797 1103 54-55 -9.15995297177119E-5 1103 56-57 0.1118329538367675 1103 58-59 0.11253079120677256 1103 60-61 -0.12007886320633077 1103 62-63 0.03654296036091864 1103 64-65 0.14387446342627186 1103 66-67 0.25187026998037254 1103 68-69 0.2860255849172759 1103 70-71 0.18137574760617525 1103 72-73 0.45105851252668216 1103 74-75 0.41517719065548775 1103 76-77 0.3831888294109369 1103 78-79 0.6710806483893066 1103 80-81 0.608398102609371 1103 82-83 0.6542456666953989 1103 84-85 0.6074750115224852 1103 86-87 0.8657125497486042 1103 88-89 0.8682082375890303 1103 90-91 0.776530578721907 1103 92-93 0.835335616728333 1103 94-95 0.6930423917640383 1103 96-97 0.41345519100775263 1103 98-99 0.5583059052238468 1103 100-101 1.0517620298578407 1104 1 0.2362554952130722 1104 2 0.13117198925866802 1104 3 0.1646253072476611 1104 4 0.01605074132344697 1104 5 0.18847864571110762 1104 6 0.18144532785586875 1104 7 0.0346547119891909 1104 8 0.230373244165321 1104 9 0.32641460040004233 1104 10-11 0.40380275349743755 1104 12-13 0.31035907838911214 1104 14-15 0.38077505982288073 1104 16-17 0.22098113846235634 1104 18-19 0.32439405017590417 1104 20-21 0.4104889197615904 1104 22-23 0.3570059378779149 1104 24-25 0.2482342308320895 1104 26-27 0.3653849334595023 1104 28-29 0.2320866616846402 1104 30-31 0.17133203826175958 1104 32-33 0.18920195862197886 1104 34-35 0.2709389365951367 1104 36-37 0.020340328367090876 1104 38-39 0.08509403487681055 1104 40-41 0.04247201758336416 1104 42-43 0.1051517022993238 1104 44-45 0.3581107531708767 1104 46-47 0.3452345009650486 1104 48-49 0.39517454658855655 1104 50-51 0.34899825971297815 1104 52-53 0.48492313244047835 1104 54-55 0.33777773842034975 1104 56-57 0.43706980299015896 1104 58-59 0.37275634413244774 1104 60-61 0.31796328203893864 1104 62-63 0.42823212764375995 1104 64-65 0.3182810430464187 1104 66-67 0.4597698764385143 1104 68-69 0.6386722853038904 1104 70-71 0.5856112242967413 1104 72-73 0.6712273087960412 1104 74-75 0.43815056538910113 1104 76-77 0.4028832225606678 1104 78-79 0.562402992622463 1104 80-81 0.5643847829280482 1104 82-83 0.7662202447367648 1104 84-85 0.8781119316697534 1104 86-87 0.8145027705344354 1104 88-89 0.5249916127199015 1104 90-91 0.7463344741584343 1104 92-93 0.7373941828314479 1104 94-95 0.961349264714471 1104 96-97 1.0105822456973854 1104 98-99 0.9356940994805178 1104 100-101 1.3634927426542944 1105 1 -0.027787902937724596 1105 2 -0.03799214612710955 1105 3 -0.15087697021036917 1105 4 -0.027116745635709094 1105 5 -0.0359614386618361 1105 6 -0.10094835139911851 1105 7 -0.2081620547707601 1105 8 -0.2153415497240303 1105 9 -0.07429156443844676 1105 10-11 -0.095855419367048 1105 12-13 -0.021156271872541765 1105 14-15 0.04666126751482835 1105 16-17 0.012079598572789507 1105 18-19 0.08656921018157249 1105 20-21 0.026674712536532752 1105 22-23 0.16812699192828973 1105 24-25 0.15322990935894865 1105 26-27 0.0172451122656625 1105 28-29 -0.03804917986319367 1105 30-31 -0.03956696710465479 1105 32-33 0.03488437620133311 1105 34-35 0.09630904089409142 1105 36-37 0.1692122988198932 1105 38-39 0.05383838986568179 1105 40-41 0.08538660804389053 1105 42-43 0.015618004535646435 1105 44-45 0.016992978437734507 1105 46-47 -0.0971196875289948 1105 48-49 0.2328122153830705 1105 50-51 0.24183197887558805 1105 52-53 0.06892645622303206 1105 54-55 0.14110694018450687 1105 56-57 -0.08802392156386674 1105 58-59 0.17532060346530187 1105 60-61 -0.10379651631084386 1105 62-63 0.1780917839824312 1105 64-65 0.19467232117420963 1105 66-67 0.09495278174684074 1105 68-69 0.14403313525845363 1105 70-71 0.03193533200285259 1105 72-73 -0.06038141863433566 1105 74-75 -0.1503041536317724 1105 76-77 -0.11882488485642284 1105 78-79 0.12685755601472337 1105 80-81 0.17791724677913479 1105 82-83 0.14590543028220182 1105 84-85 -0.05206709681862165 1105 86-87 0.02892912624031041 1105 88-89 -0.041711923106639404 1105 90-91 0.0756348957452353 1105 92-93 0.04587163474637279 1105 94-95 0.1807091864555126 1105 96-97 0.2910368202059743 1105 98-99 0.5129275785304053 1105 100-101 0.6172229660091482 1106 1 0.05028341150761406 1106 2 0.10219323696620464 1106 3 -0.18201600904195914 1106 4 0.037530771025942045 1106 5 0.01615639387966894 1106 6 -0.033070537359641605 1106 7 0.09346261702225434 1106 8 -0.006601154076847138 1106 9 0.008015263929515015 1106 10-11 -0.1296125560266148 1106 12-13 -0.1867520931949258 1106 14-15 -0.14602702919486887 1106 16-17 -0.2539913954125339 1106 18-19 -0.044538614030990686 1106 20-21 -0.014818056706559446 1106 22-23 0.014868125238137964 1106 24-25 0.06186517295999039 1106 26-27 0.1350440205853758 1106 28-29 0.13597874550590205 1106 30-31 0.31901909379181603 1106 32-33 0.4037228900529186 1106 34-35 0.4517963139456711 1106 36-37 0.24241209203248815 1106 38-39 -0.039764653899780456 1106 40-41 0.19395982030782477 1106 42-43 0.0029719269208428045 1106 44-45 0.018266126760835277 1106 46-47 0.26917215177542886 1106 48-49 0.17778794658363495 1106 50-51 0.11445781611515571 1106 52-53 0.05963424959887931 1106 54-55 0.21241496120790515 1106 56-57 -0.025604131041923495 1106 58-59 -0.11129195282304494 1106 60-61 -0.3612899143811319 1106 62-63 0.09633820261506543 1106 64-65 0.19175303628374962 1106 66-67 0.06701116290983578 1106 68-69 0.07453110290710896 1106 70-71 -0.08912146046452207 1106 72-73 0.007843308483383993 1106 74-75 0.09334068597950562 1106 76-77 0.216180156342336 1106 78-79 0.10439323364404629 1106 80-81 0.15201402570254174 1106 82-83 0.049089850483877484 1106 84-85 0.18333874613481171 1106 86-87 -0.09450655274945774 1106 88-89 0.175191511106096 1106 90-91 0.37025555120688836 1106 92-93 0.3367491812319692 1106 94-95 0.23471501217666102 1106 96-97 0.4785471180070431 1106 98-99 0.44508831038851326 1106 100-101 0.8202030173734443 1107 1 -0.130380158268121 1107 2 -0.19935346867256953 1107 3 -0.2833087000313981 1107 4 -0.2586224728803188 1107 5 -0.23850199121349647 1107 6 0.007710992643666259 1107 7 -0.30596671992991276 1107 8 -0.02586103194103373 1107 9 -0.2806045318148662 1107 10-11 -0.1620168220808651 1107 12-13 0.08740104632175161 1107 14-15 -0.007041000000576503 1107 16-17 -0.08216614357267105 1107 18-19 -0.2539789118892699 1107 20-21 -0.17925774562560548 1107 22-23 -0.1341480029522728 1107 24-25 -0.12869800883446914 1107 26-27 -0.2943625246414143 1107 28-29 -0.28678166300532837 1107 30-31 -0.1447142256228986 1107 32-33 -0.157762573697795 1107 34-35 -0.2443952061406165 1107 36-37 -0.2899486783626273 1107 38-39 -0.1450241490021682 1107 40-41 -0.21167199763674205 1107 42-43 -0.013199759144889356 1107 44-45 0.09488807988991255 1107 46-47 0.1448006640499031 1107 48-49 0.027092885950686707 1107 50-51 -0.24518169186195138 1107 52-53 -0.0519669126051312 1107 54-55 0.2874014775535656 1107 56-57 0.14742160099001467 1107 58-59 0.02799854598517726 1107 60-61 0.10517493242716114 1107 62-63 0.43094160919508795 1107 64-65 0.09556673567654883 1107 66-67 0.24307289194928217 1107 68-69 0.2354911390954939 1107 70-71 0.4519906365481958 1107 72-73 0.45047253408666776 1107 74-75 0.38225625220599113 1107 76-77 0.3634999538265511 1107 78-79 0.5774694206405826 1107 80-81 0.3737200994363832 1107 82-83 0.42955508005396936 1107 84-85 0.5247100772759623 1107 86-87 0.33302098000277525 1107 88-89 0.3203974195564321 1107 90-91 0.2908411767614574 1107 92-93 0.5023719520794394 1107 94-95 0.5606018886663868 1107 96-97 0.6231788837568217 1107 98-99 0.6357993774240214 1107 100-101 0.5668929977134098 1108 1 -0.2651569560337812 1108 2 -0.23053094697904442 1108 3 -0.18809550877898218 1108 4 -0.10075200954323549 1108 5 5.346595724091685E-4 1108 6 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-0.8904692881830805 2316 86-87 -0.8236930349745002 2316 88-89 -0.5333809388310264 2316 90-91 -0.8037787794474873 2316 92-93 -1.0279214602495195 2316 94-95 -0.8974592838789519 2316 96-97 -0.985164876017965 2316 98-99 -0.9664119481446747 2316 100-101 -1.31874580582177 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3504.0 3 634.0 4 478.0 5 602.0 6 695.0 7 915.0 8 1096.0 9 1396.0 10 1858.0 11 2462.0 12 3603.0 13 4766.0 14 5548.0 15 7349.0 16 9767.0 17 13762.0 18 10875.0 19 6418.0 20 6129.0 21 6287.0 22 6890.0 23 7492.0 24 8481.0 25 9370.0 26 10990.0 27 12724.0 28 15024.0 29 18253.0 30 22258.0 31 27498.0 32 34955.0 33 45432.0 34 62232.0 35 90404.0 36 129042.0 37 181478.0 38 152123.0 39 21868.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.026017213774 19.42047740014853 15.091322672735366 21.46218271334211 2 14.79636754837127 20.85951596339512 41.08958680915823 23.254529679075382 3 18.565601147824943 26.964115602369144 30.097163678689732 24.373119571116185 4 11.792736869494108 16.84389951200344 38.18225264309344 33.18111097540901 5 12.63021980880743 38.724299804730016 33.69463766746339 14.950842718999164 6 29.754223341302 37.27564908572526 18.016194117191418 14.953933455781318 7 27.477903387209913 34.11946231663976 20.91688872051353 17.485745575636795 8 26.733453877138697 34.145836781989736 19.7706696547948 19.350039686076766 9 27.379407454725076 16.39213971852985 20.51465977086382 35.71379305588126 10-11 24.414271009094477 27.52029543790392 27.090456634087843 20.974976918913757 12-13 26.063275765372314 25.189117888480272 27.91012330534083 20.837483040806593 14-15 22.988253305896265 25.74028893801249 27.388369802036138 23.88308795405511 16-17 20.7252808356838 29.464666594507865 27.799761451495492 22.010291118312843 18-19 20.781925641892123 27.147027633231176 31.609012776039208 20.462033948837487 20-21 21.884806310203945 26.003967220298502 31.492556554640526 20.618669914857023 22-23 21.45461953321372 26.110917179759507 31.428444105860347 21.006019181166423 24-25 21.052578533614827 26.481980500535755 31.540807774323937 20.924633191525476 26-27 20.913192055130438 26.516376199770907 31.619623745051967 20.95080800004669 28-29 20.587537781541123 26.59624192707214 31.89984980229185 20.91637048909488 30-31 20.74896454680418 26.584484809638976 31.652183233033476 21.014367410523366 32-33 20.562544769585863 26.661926617674368 31.52251081102592 21.253017801713845 34-35 21.009233498649248 26.649445374655944 31.36365469134309 20.977666435351715 36-37 21.183975146580426 26.34946301589626 31.245238656064917 21.2213231814584 38-39 20.853525794720113 26.728471158375378 31.23644700380651 21.181556043098 40-41 20.7817627934634 26.753274584513797 31.342016050277927 21.12294657174487 42-43 20.724491230303656 26.882295371541787 30.810344873314822 21.58286852483974 44-45 20.83959381167632 26.92647651790072 30.51739139661722 21.716538273805735 46-47 21.197962089431257 27.31429317138692 29.98586788381109 21.501876855370735 48-49 21.113937132864756 27.49126727445588 29.483771773454215 21.911023819225154 50-51 21.170271301356163 27.669384933173518 29.407313903803185 21.753029861667137 52-53 21.36454378828032 27.937004564717423 28.97093800113985 21.72751364586241 54-55 21.01248479728654 27.830888517679718 29.02131669825718 22.13530998677656 56-57 21.169855415002278 28.01779213897642 28.766574710912217 22.04577773510908 58-59 21.346725385501454 27.50223271580975 29.08316521672723 22.067876681961565 60-61 21.382594702735616 27.703394352257792 29.32451339909389 21.5894975459127 62-63 21.39551288851172 27.76636257558078 29.328259255330437 21.509865280577063 64-65 21.256570049590284 27.76615662043544 29.30329629531412 21.67397703466016 66-67 21.247852357857784 27.926747566712834 28.842827339385014 21.98257273604437 68-69 21.27270470700678 27.849657526617666 29.027602601461123 21.850035164914434 70-71 21.369040957403644 27.909050260720957 28.94806213597589 21.77384664589951 72-73 21.336891161749456 27.762891602057245 28.787270330416515 22.112946905776784 74-75 20.97317207332553 28.349432989061853 28.642658721423352 22.034736216189263 76-77 20.998439258950388 27.945858943385737 28.971268996001065 22.08443280166281 78-79 21.06653878330082 27.76195445109973 28.92680357189275 22.244703193706695 80-81 20.954250543146173 28.32533819336004 28.58768203883495 22.13272922465884 82-83 21.04972788938819 27.97606890745693 28.710839174379426 22.263364028775452 84-85 20.993207518349564 27.80080478735507 28.443351169763247 22.762636524532116 86-87 20.948287247454992 27.88702617064036 28.58365816129253 22.581028420612117 88-89 21.07013653868596 27.462417381894568 28.692909960852546 22.774536118566928 90-91 20.85707071671515 27.441263900293443 29.010954736125168 22.69071064686624 92-93 20.874139191341147 27.406312296235942 29.09090330404117 22.628645208381737 94-95 20.587136925058406 27.24443916339725 29.099518976759388 23.06890493478496 96-97 20.626750403123143 27.221738945939066 29.0736654502249 23.07784520071289 98-99 20.56820394104819 27.30449257426399 28.806667752354876 23.320635732332946 100-101 20.624048988779062 27.12473607698358 29.200320552767895 23.050894381469462 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1917.0 1 1174.5 2 562.0 3 651.0 4 525.5 5 532.0 6 925.0 7 2004.5 8 3138.0 9 3414.0 10 2874.0 11 2338.5 12 2284.0 13 2325.0 14 2365.5 15 2358.0 16 2344.5 17 2418.5 18 2497.0 19 2531.0 20 2669.5 21 2890.0 22 3225.5 23 3802.0 24 4419.5 25 5090.0 26 6237.0 27 7424.5 28 8232.0 29 9569.5 30 11566.5 31 13430.0 32 15624.5 33 18095.5 34 20764.5 35 22812.5 36 25574.0 37 28717.0 38 29694.5 39 29772.0 40 29448.0 41 29760.0 42 30077.5 43 30817.0 44 32488.0 45 33662.0 46 33248.5 47 33349.5 48 36820.5 49 36508.5 50 33104.5 51 30239.5 52 27349.0 53 26647.5 54 26203.0 55 24639.5 56 22513.5 57 20417.5 58 18817.5 59 16954.5 60 14492.0 61 12355.5 62 10012.0 63 7216.5 64 4977.0 65 3666.0 66 2804.0 67 2218.0 68 1673.5 69 1198.0 70 835.0 71 614.0 72 486.0 73 358.5 74 253.5 75 201.0 76 140.5 77 81.5 78 46.5 79 29.5 80 21.5 81 18.0 82 11.0 83 4.5 84 5.5 85 6.5 86 9.0 87 6.5 88 3.5 89 3.5 90 3.5 91 3.0 92 1.5 93 2.0 94 1.5 95 0.5 96 1.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36394008009222356 2 0.3215968479698008 3 0.32329057725469773 4 0.32350229341530984 5 0.3057181359238923 6 0.23500493827944627 7 0.24580246247066406 8 0.23976855189321886 9 0.2383923968492401 10-11 0.2467551851934186 12-13 0.24617296575173528 14-15 0.22658922089511474 16-17 0.24294429430240055 18-19 0.22807123401939958 20-21 0.23156455066949944 22-23 0.23013546658536768 24-25 0.2192320843138438 26-27 0.23728088700602648 28-29 0.2367515966044962 30-31 0.24559074631005195 32-33 0.24728447559494887 34-35 0.23532251252036443 36-37 0.23024132466567374 38-39 0.21859693583200746 40-41 0.2181205744706302 42-43 0.21896743911307864 44-45 0.23722795796587348 46-47 0.22516013681098296 48-49 0.23595766100220078 50-51 0.24691397231387768 52-53 0.2576585674649425 54-55 0.25353010233300627 56-57 0.21145151541134863 58-59 0.19721360361018397 60-61 0.20621154043619883 62-63 0.20758769548017753 64-65 0.2049412434725261 66-67 0.21780300022971205 68-69 0.20748183739987147 70-71 0.210287076527982 72-73 0.22637750473450263 74-75 0.21621512902512116 76-77 0.22865345346108285 78-79 0.22605993049358447 80-81 0.21224545101364406 82-83 0.2245779173692997 84-85 0.20404144978992464 86-87 0.20986364420675774 88-89 0.2192320843138438 90-91 0.2175383550289469 92-93 0.22103167167904675 94-95 0.2209258135987407 96-97 0.21393918029854095 98-99 0.22171974920103615 100-101 0.20266529474594588 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 944661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.59633975513536 #Duplication Level Percentage of deduplicated Percentage of total 1 75.91896880987137 39.93059877383519 2 13.060183696935065 13.738357179769528 3 4.488572325283093 7.082474251082626 4 2.0956953338579067 4.409036152113691 5 1.1369812775589605 2.990052678485947 6 0.70250126812255 2.2169397225952245 7 0.4642833483599579 1.7093723313094544 8 0.3365485852755978 1.4160978988212387 9 0.24202344008818552 1.1456592375226369 >10 1.3929826597195343 13.600565880383838 >50 0.10556571167868209 3.804634839881645 >100 0.051873408578527264 4.645071139025531 >500 0.0022116569145585237 0.7171968606785637 >1k 0.001206358317031922 1.1422063244159784 >5k 4.0211943901064066E-4 1.4517367300789328 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7960 0.8426303192362128 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5768 0.6105894072053362 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4438 0.46979816039828043 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1521 0.16101014014551251 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1454 0.1539176487650067 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1228 0.12999372261583783 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1144 0.12110164387012906 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1016 0.10755180959095378 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 1.0585808030605688E-4 0.0 5 0.0 0.0 0.0 1.0585808030605688E-4 0.0 6 0.0 0.0 0.0 1.0585808030605688E-4 0.0 7 0.0 0.0 0.0 1.0585808030605688E-4 0.0 8 0.0 0.0 0.0 1.0585808030605688E-4 0.0 9 0.0 0.0 0.0 1.0585808030605688E-4 0.0 10-11 0.0 0.0 0.0 1.0585808030605688E-4 0.0 12-13 0.0 0.0 0.0 1.0585808030605688E-4 0.0 14-15 0.0 0.0 0.0 1.0585808030605688E-4 0.0 16-17 0.0 0.0 0.0 2.646452007651422E-4 0.0 18-19 0.0 0.0 0.0 4.234323212242275E-4 0.0 20-21 0.0 0.0 0.0 4.234323212242275E-4 0.0 22-23 0.0 0.0 0.0 7.410065621423982E-4 0.0 24-25 0.0 0.0 0.0 0.0011115098432135971 0.0 26-27 0.0 0.0 0.0 0.0012173679235196542 0.0 28-29 0.0 0.0 0.0 0.001958374485662052 0.0 30-31 0.0 0.0 0.0 0.0031228133690286777 0.0 32-33 0.0 0.0 0.0 0.004710684573619532 0.0 34-35 0.0 0.0 0.0 0.006139768657751299 0.0 36-37 0.0 0.0 0.0 0.008309859304025466 0.0 38-39 0.0 0.0 0.0 0.01010944666922843 0.0 40-41 0.0 0.0 0.0 0.01397326660039951 0.0 42-43 0.0 0.0 0.0 0.019848390057385666 0.0 44-45 0.0 0.0 0.0 0.031069346569827697 0.0 46-47 0.0 0.0 0.0 0.057533866646341913 0.0 48-49 0.0 0.0 0.0 0.07717054054311547 0.0 50-51 0.0 0.0 0.0 0.10792231287202499 0.0 52-53 0.0 0.0 0.0 0.1457136475412873 0.0 54-55 0.0 0.0 0.0 0.1857280018969768 0.0 56-57 0.0 0.0 0.0 0.2354283706006705 0.0 58-59 0.0 0.0 0.0 0.3029128967957818 0.0 60-61 0.0 0.0 0.0 0.38590563175573034 0.0 62-63 0.0 0.0 0.0 0.4937220865474493 0.0 64-65 0.0 0.0 0.0 0.5831192353659143 0.0 66-67 0.0 0.0 0.0 0.7149125453469551 0.0 68-69 0.0 0.0 0.0 0.8820624541502189 0.0 70-71 0.0 0.0 0.0 1.0151789901350856 0.0 72-73 0.0 0.0 0.0 1.2458437471219834 0.0 74-75 0.0 0.0 0.0 1.435276781829672 0.0 76-77 0.0 0.0 0.0 1.601950329271559 0.0 78-79 0.0 5.292904015302844E-5 0.0 1.81991211662173 0.0 80-81 0.0 1.0585808030605688E-4 0.0 2.054969983941329 0.0 82-83 0.0 1.0585808030605688E-4 0.0 2.330306850817383 0.0 84-85 0.0 1.0585808030605688E-4 0.0 2.709119991192608 0.0 86-87 0.0 1.0585808030605688E-4 0.0 3.2014659226960784 0.0 88-89 0.0 1.0585808030605688E-4 0.0 3.7882901908727047 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5655 0.0 31.622736 1 GTATCAA 8460 0.0 31.42575 1 TATAACG 65 0.0058149933 29.260084 2 GTACATG 7090 0.0 28.039757 1 ACGACGG 85 6.40456E-4 27.942488 6 TACATGG 7230 0.0 26.897623 2 CGACGGT 90 8.943896E-4 26.390127 7 ACATGGG 7020 0.0 25.60121 3 TATCAAC 10445 0.0 25.401224 2 ATCAACG 10365 0.0 25.366566 3 TCAACGC 10440 0.0 25.184334 4 CAACGCA 10485 0.0 24.938885 5 AACGCAG 10775 0.0 24.379335 6 GAGTACT 5575 0.0 24.324965 12-13 CATGGGA 5270 0.0 23.727444 4 ATGGGAT 1885 0.0 23.708237 5 GTCGGGA 365 0.0 23.448147 2 ATGGGAG 1915 0.0 23.33683 5 AGTACTT 5870 0.0 22.657442 12-13 TACCTGG 550 0.0 22.477062 2 >>END_MODULE