FastQCFastQC Report
Thu 26 May 2016
SRR937365_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937365_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences944661
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82480.8731174463643571No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64500.6827846179740669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50730.5370180413926265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15800.16725576688356988No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT15540.1645034567956124No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14710.15571723613020966No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13400.14184982761011622No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA92650.027.852591
GGTATCA65100.026.7184541
GTACATG72950.024.6250841
TACCTGG6650.024.2837372
TACATGG75150.023.763872
ACATGGG74050.023.475473
AGTACTT63600.023.45431712-13
CATGGGG20200.023.2778114
GAGTACT62150.022.77851312-13
ATCAACG114000.022.5398333
TCAACGC114750.022.4339074
TAAGGTG3200.022.2638135
TATCAAC116100.022.2139532
CAACGCA116050.022.18265
AACGCAG118250.021.76996
GTACTTT67100.021.05827114-15
CATGGGA55150.020.1524854
AGGACCG1908.254494E-619.9983755
ACGCAGA129050.019.8786097
TACTTTT74450.019.84056314-15
Produced by FastQC (version 0.11.5)