##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937365_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 944661 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.20964451798052 33.0 31.0 34.0 31.0 34.0 2 32.4151573950867 34.0 31.0 34.0 31.0 34.0 3 32.48759502085934 34.0 31.0 34.0 31.0 34.0 4 36.101927569784294 37.0 35.0 37.0 35.0 37.0 5 36.00469904018479 37.0 35.0 37.0 35.0 37.0 6 36.09871054272379 37.0 35.0 37.0 35.0 37.0 7 36.050585342255054 37.0 35.0 37.0 35.0 37.0 8 36.038566215817106 37.0 35.0 37.0 35.0 37.0 9 37.72946908996984 39.0 37.0 39.0 35.0 39.0 10-11 37.660867761027504 39.0 37.0 39.0 35.0 39.0 12-13 37.582225793168135 39.0 37.0 39.0 35.0 39.0 14-15 38.75566631839358 40.0 38.0 41.0 35.0 41.0 16-17 38.74549176900497 40.0 38.0 41.0 35.0 41.0 18-19 38.792755284700014 40.0 38.0 41.0 35.0 41.0 20-21 38.708649452025654 40.0 38.0 41.0 34.5 41.0 22-23 38.590085226340456 40.0 38.0 41.0 34.0 41.0 24-25 38.448162356654926 40.0 38.0 41.0 34.0 41.0 26-27 38.36146141314185 40.0 38.0 41.0 34.0 41.0 28-29 38.25794756002418 40.0 38.0 41.0 34.0 41.0 30-31 38.2792266220369 40.0 37.5 41.0 34.0 41.0 32-33 38.154072730852654 40.0 37.0 41.0 34.0 41.0 34-35 38.02806085992753 40.0 37.0 41.0 34.0 41.0 36-37 37.846511605750635 40.0 37.0 41.0 33.0 41.0 38-39 37.69847066831382 40.0 37.0 41.0 33.0 41.0 40-41 37.51163009799282 40.0 36.5 41.0 33.0 41.0 42-43 37.25092811071909 40.0 36.0 41.0 32.5 41.0 44-45 36.865406743794864 40.0 36.0 41.0 31.5 41.0 46-47 36.39968147303636 39.5 36.0 41.0 30.5 41.0 48-49 36.00237386745086 39.0 35.0 41.0 29.5 41.0 50-51 35.76638762476698 39.0 35.0 41.0 28.5 41.0 52-53 35.52695252582673 39.0 35.0 41.0 28.0 41.0 54-55 35.29806247955616 39.0 35.0 40.0 27.0 41.0 56-57 35.04854704491876 38.0 35.0 40.0 26.5 41.0 58-59 34.806587230763206 38.0 34.5 40.0 26.0 41.0 60-61 34.47564629004479 38.0 34.0 40.0 26.0 41.0 62-63 34.10312800041496 37.0 34.0 40.0 25.0 41.0 64-65 33.74841080556941 37.0 33.5 39.5 24.5 41.0 66-67 33.3276503422921 36.0 33.0 39.0 22.5 41.0 68-69 32.9172306255895 36.0 33.0 39.0 22.0 40.0 70-71 32.5082675160719 35.0 32.5 38.5 20.5 40.0 72-73 32.076939769928046 35.0 32.0 37.5 20.0 40.0 74-75 31.628199957445055 35.0 31.5 37.0 18.0 39.0 76-77 30.291801503396456 33.5 29.5 35.5 16.0 38.5 78-79 30.911460830922415 34.5 31.0 36.0 16.0 38.5 80-81 30.77129308820836 35.0 31.0 36.0 12.0 37.5 82-83 30.454124813028166 34.0 31.0 35.0 7.5 37.0 84-85 30.093486446460687 34.0 31.0 35.0 4.5 36.5 86-87 29.73777947856427 34.0 30.5 35.0 2.0 36.0 88-89 29.349974223557446 34.0 30.0 35.0 2.0 36.0 90-91 28.92240285139325 34.0 29.0 35.0 2.0 35.0 92-93 28.570654446409876 34.0 29.0 35.0 2.0 35.0 94-95 28.16910722470812 34.0 29.0 35.0 2.0 35.0 96-97 27.71898914001954 33.5 28.5 35.0 2.0 35.0 98-99 27.26524224033807 33.0 27.0 35.0 2.0 35.0 100-101 25.446257969790224 31.5 22.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07337618140746116 1101 2 0.1407392842325379 1101 3 0.12815419752276824 1101 4 0.059846722287673515 1101 5 0.007153650099652964 1101 6 -0.10270814712600895 1101 7 -0.011341031226031362 1101 8 -0.013570836273416376 1101 9 0.13860640065864516 1101 10-11 -0.05423624763953683 1101 12-13 -0.03478424934727542 1101 14-15 -0.12674750467343188 1101 16-17 0.005339329192914022 1101 18-19 -0.1232323435392857 1101 20-21 -0.0699694295512785 1101 22-23 -0.10406007215905788 1101 24-25 0.034015462857205137 1101 26-27 -0.16239836244112382 1101 28-29 -0.17866094096166307 1101 30-31 -0.09685317517638481 1101 32-33 -0.009855769875194653 1101 34-35 -0.07878856616351726 1101 36-37 -0.04550320702742994 1101 38-39 -0.1570028336221796 1101 40-41 -0.12067737843324977 1101 42-43 -0.0893751966935028 1101 44-45 0.12181271263990112 1101 46-47 0.1696537732588297 1101 48-49 0.2785955958959718 1101 50-51 0.09038634089863962 1101 52-53 0.14920851338047214 1101 54-55 0.06879248769733692 1101 56-57 0.18030170855691807 1101 58-59 0.13244142324377606 1101 60-61 0.1726900912748377 1101 62-63 0.06721950001800536 1101 64-65 0.0016899816133317813 1101 66-67 0.1824659227467862 1101 68-69 0.1446720069480989 1101 70-71 -0.15030851052291183 1101 72-73 -0.09862075119210445 1101 74-75 0.049612952302314994 1101 76-77 -0.10600096671328174 1101 78-79 0.11853546263882819 1101 80-81 0.12653305123589575 1101 82-83 -0.09143063501460702 1101 84-85 0.031706799624490856 1101 86-87 -0.07452370924145768 1101 88-89 -0.031327371776516344 1101 90-91 0.12644212348100226 1101 92-93 -0.16760394201049778 1101 94-95 -0.3863245953824723 1101 96-97 -0.2763820365109737 1101 98-99 -0.2682884401832659 1101 100-101 0.08583672385803709 1102 1 8.713452035067348E-4 1102 2 0.001988495474556373 1102 3 0.030067688750222032 1102 4 -0.0010019877772009522 1102 5 -0.14308482366823227 1102 6 -0.01588192783898279 1102 7 -0.007615890869217878 1102 8 -0.010973603631597939 1102 9 0.03271588480120613 1102 10-11 -0.013982057018601779 1102 12-13 8.838076065629252E-4 1102 14-15 -0.04065529999932238 1102 16-17 -0.06098857644895617 1102 18-19 -0.00824309137902901 1102 20-21 -0.048801562417644107 1102 22-23 0.008645183074598606 1102 24-25 0.037958280980952 1102 26-27 -0.03380943265127456 1102 28-29 -0.11199550521094181 1102 30-31 -0.06831438901499354 1102 32-33 -0.002066663351726561 1102 34-35 -0.18272030604376965 1102 36-37 -0.0784088294627523 1102 38-39 0.0036155008060205773 1102 40-41 -0.0027614616535274195 1102 42-43 -0.09221116224340165 1102 44-45 -0.05105388328149729 1102 46-47 -0.2724682351295371 1102 48-49 -0.14292930164646833 1102 50-51 -0.259194111119605 1102 52-53 -0.1733086319829269 1102 54-55 -0.2371380653208064 1102 56-57 -0.3694525617496325 1102 58-59 -0.07833738375249055 1102 60-61 0.010772168749980437 1102 62-63 -0.17006287080383942 1102 64-65 -0.12416923010810166 1102 66-67 0.10485246145002947 1102 68-69 0.05280777827242389 1102 70-71 -0.03027379869329394 1102 72-73 0.012258861341472027 1102 74-75 -0.31784428632564143 1102 76-77 -0.27252607804638984 1102 78-79 -0.12610001132211934 1102 80-81 -0.07644207260072733 1102 82-83 -0.06276065912760131 1102 84-85 -0.1741337669596419 1102 86-87 -0.28200268973208864 1102 88-89 -0.062004712427967235 1102 90-91 0.010650184555160536 1102 92-93 0.10016141431375303 1102 94-95 -0.05247006632443174 1102 96-97 -0.26467570195838874 1102 98-99 -0.1495743715914486 1102 100-101 0.12644269729876 1103 1 -0.04224376288087228 1103 2 -0.06865256747278181 1103 3 0.0030225529531833217 1103 4 -0.015367862362104745 1103 5 -0.09418177802289307 1103 6 -0.11972510838841544 1103 7 -0.05529191123596888 1103 8 -0.08119629154755614 1103 9 -0.12720391208083726 1103 10-11 -0.2443274849288386 1103 12-13 -0.13566256270905086 1103 14-15 -0.06920034150586929 1103 16-17 -0.08228105556455034 1103 18-19 -0.15891898560882822 1103 20-21 -0.1504766860880551 1103 22-23 -0.16009920476779627 1103 24-25 -0.11686858044448911 1103 26-27 -0.08660118054722687 1103 28-29 -0.2742366590767915 1103 30-31 -0.32884307767778154 1103 32-33 -0.1288598999134578 1103 34-35 -0.22601056964501964 1103 36-37 -0.30765212016346055 1103 38-39 -0.35272637778529514 1103 40-41 -0.19280675855618057 1103 42-43 -0.3282093363798353 1103 44-45 -0.32397053173531276 1103 46-47 -0.18641941888110125 1103 48-49 0.07135583280177826 1103 50-51 -0.1364469357755027 1103 52-53 -0.20313221509655932 1103 54-55 -0.23709123229490814 1103 56-57 -0.34330724449022654 1103 58-59 -0.21814895263387513 1103 60-61 -0.28136475353341694 1103 62-63 -0.3202207917671771 1103 64-65 -0.23154674301215294 1103 66-67 -0.17141836831878976 1103 68-69 -0.11071909826448234 1103 70-71 -0.10503398237866435 1103 72-73 -0.08080960104911128 1103 74-75 -0.10463318927137877 1103 76-77 -0.1426802648564589 1103 78-79 -0.09801081126766675 1103 80-81 -0.10686948952830377 1103 82-83 -0.2200809108519941 1103 84-85 -0.22862773923698754 1103 86-87 -0.12046278237405872 1103 88-89 -0.11441118063084232 1103 90-91 -0.2457203558480039 1103 92-93 0.17267798383831945 1103 94-95 0.22372770392398067 1103 96-97 0.02254156706425192 1103 98-99 0.082628766346172 1103 100-101 0.24889544902002925 1104 1 0.13150413709720965 1104 2 0.09223506101140089 1104 3 0.06012264534773948 1104 4 -0.020784028718438208 1104 5 -0.04341731237995816 1104 6 0.05389272158253533 1104 7 0.018872981651703924 1104 8 0.07127715302659965 1104 9 0.08630440278853513 1104 10-11 0.020485118150041615 1104 12-13 -0.08816714719624485 1104 14-15 0.0022109757380803785 1104 16-17 0.024295296811118305 1104 18-19 -0.05416841887522139 1104 20-21 -0.07801103289954625 1104 22-23 -0.018844138124535448 1104 24-25 0.03521369299982524 1104 26-27 0.14158614332965413 1104 28-29 0.07832834709353165 1104 30-31 -0.015981781001549678 1104 32-33 0.08404259627734945 1104 34-35 0.19438530450872094 1104 36-37 0.12191497097140314 1104 38-39 0.15173636901133847 1104 40-41 0.19281685344306254 1104 42-43 0.21374977422323838 1104 44-45 0.3240430493675035 1104 46-47 0.4000829025161181 1104 48-49 0.38316535648925054 1104 50-51 0.14895004685661917 1104 52-53 0.29041586403213415 1104 54-55 0.5176521581624414 1104 56-57 0.33010939344997325 1104 58-59 0.4072180306005464 1104 60-61 0.3166987411337132 1104 62-63 0.2777257680413143 1104 64-65 0.3142282884160892 1104 66-67 0.17220800910976664 1104 68-69 0.41707807966487564 1104 70-71 0.2202118371200399 1104 72-73 0.3372581481246577 1104 74-75 0.22544732598526096 1104 76-77 0.2851738106917914 1104 78-79 0.4251647166051207 1104 80-81 0.5056401055374948 1104 82-83 0.6730521616578677 1104 84-85 0.7433956759272533 1104 86-87 0.6098684540342667 1104 88-89 0.49130305748569114 1104 90-91 0.28628968068693084 1104 92-93 0.32163857711132593 1104 94-95 0.4299283910524352 1104 96-97 0.4070435655290403 1104 98-99 0.49020121149571594 1104 100-101 0.792117776465826 1105 1 -0.045841616881737934 1105 2 0.02429344397451416 1105 3 -9.379636603767949E-4 1105 4 0.06306227690836863 1105 5 -0.06610018294975362 1105 6 -0.12774115654358553 1105 7 -0.03242610041738203 1105 8 -0.037205718099009744 1105 9 -0.0347149090550829 1105 10-11 0.05202006094263112 1105 12-13 -0.030707534211259713 1105 14-15 0.02526624334981875 1105 16-17 -0.061337958800542935 1105 18-19 -0.08478626447496396 1105 20-21 -0.03421755762921208 1105 22-23 -0.2287100196077887 1105 24-25 -0.12027874650649295 1105 26-27 -0.11827126195226612 1105 28-29 -0.1074088461999878 1105 30-31 -0.039049554484101634 1105 32-33 -0.15693580747329605 1105 34-35 -0.1287955776276064 1105 36-37 -0.17369880327261455 1105 38-39 -0.010277187352620842 1105 40-41 0.10992585739214178 1105 42-43 0.10309018680582227 1105 44-45 0.07796502940813355 1105 46-47 -0.1439544372350383 1105 48-49 0.15656314600710886 1105 50-51 0.16692167798345992 1105 52-53 0.054124382753421685 1105 54-55 0.17697278367830904 1105 56-57 0.23626742319171257 1105 58-59 0.22735998612967023 1105 60-61 0.20922676123203132 1105 62-63 0.1845757893012987 1105 64-65 0.11635275795872957 1105 66-67 0.08149598040740358 1105 68-69 0.11434985447027657 1105 70-71 0.2459190441144372 1105 72-73 0.3988572043482783 1105 74-75 0.3829740538102655 1105 76-77 0.34268275238941825 1105 78-79 0.35418764681402237 1105 80-81 0.2612163343782541 1105 82-83 0.4763403719565922 1105 84-85 0.6033591032028376 1105 86-87 0.5676687825218067 1105 88-89 0.44778679568501545 1105 90-91 0.33945283227754075 1105 92-93 0.47081207800480485 1105 94-95 0.5293368633630458 1105 96-97 0.7680206631109598 1105 98-99 0.8067887206319497 1105 100-101 0.9156750853135911 1106 1 -0.18439900265362041 1106 2 -0.06921436874986853 1106 3 -0.07030924228070745 1106 4 0.021399452234760474 1106 5 -0.1374781156501257 1106 6 -0.10779554404927438 1106 7 -0.051058884988137265 1106 8 -0.07358548739803439 1106 9 -0.2591087181304488 1106 10-11 -0.1786023117009634 1106 12-13 -0.30740472519786266 1106 14-15 -0.23161911208114105 1106 16-17 -0.043461354044133316 1106 18-19 -0.1128178237396611 1106 20-21 -0.1253381700101528 1106 22-23 -0.0863373457771317 1106 24-25 -0.03084432819432692 1106 26-27 -0.0040129076958166365 1106 28-29 -0.03546066095596245 1106 30-31 0.10841117782748455 1106 32-33 -0.026324545117830667 1106 34-35 -0.06626279471961283 1106 36-37 -0.008263967757784485 1106 38-39 0.028379813111435226 1106 40-41 -0.12121612906691581 1106 42-43 -0.37231979794210446 1106 44-45 -0.3006014108706694 1106 46-47 -0.04098398455504082 1106 48-49 0.14882864712645727 1106 50-51 0.10051087545787141 1106 52-53 0.18383246182239787 1106 54-55 -0.06945454067710699 1106 56-57 0.1629300740308821 1106 58-59 0.041147778114840605 1106 60-61 0.1291767573774436 1106 62-63 0.18218707735403683 1106 64-65 -0.05492367054467451 1106 66-67 -0.13219265036673988 1106 68-69 -0.19740029555640604 1106 70-71 -0.20372943241564911 1106 72-73 -0.1933385362544655 1106 74-75 -0.19369298067999807 1106 76-77 -0.08201897038546235 1106 78-79 -0.12747470926239757 1106 80-81 -0.1529479981201689 1106 82-83 -0.1698770662074729 1106 84-85 -0.220239753420028 1106 86-87 -0.11580213195315991 1106 88-89 -0.053518474219405476 1106 90-91 -0.26281207931630846 1106 92-93 -0.03394569503997502 1106 94-95 -0.5805427525081903 1106 96-97 -0.09361468060507505 1106 98-99 0.18142876997069735 1106 100-101 0.8507498114487682 1107 1 -0.07555546523865075 1107 2 -0.04440444478027672 1107 3 -0.09772890579829152 1107 4 -0.01950386561411932 1107 5 -0.1343638264561875 1107 6 -0.12180114629016714 1107 7 -0.15602260960842074 1107 8 -0.061928650750331826 1107 9 -0.18927208782615423 1107 10-11 -0.10849600831004835 1107 12-13 -0.21181866468671018 1107 14-15 -0.2341070637835685 1107 16-17 -0.18139478828739186 1107 18-19 -0.2615033849206583 1107 20-21 -0.32605323864630265 1107 22-23 -0.10991199302733179 1107 24-25 -0.1668987499630319 1107 26-27 -0.14450388669610703 1107 28-29 -0.36901147258496536 1107 30-31 -0.2353785119614642 1107 32-33 -0.25585113836383044 1107 34-35 -0.025333379475263484 1107 36-37 -0.13650656914616377 1107 38-39 -0.14142724014464392 1107 40-41 -0.02385544566311637 1107 42-43 0.03388616270302691 1107 44-45 0.07275662799705174 1107 46-47 0.06507583067539713 1107 48-49 0.14554907442781229 1107 50-51 0.1489128449413144 1107 52-53 0.22294375851499382 1107 54-55 0.302215866616244 1107 56-57 0.2590467815834714 1107 58-59 0.32878457196281374 1107 60-61 0.42967956719702016 1107 62-63 0.38636440045718956 1107 64-65 0.23808048764033884 1107 66-67 0.24374903065349685 1107 68-69 0.3743153472224492 1107 70-71 0.3705176577247542 1107 72-73 0.35741219882217834 1107 74-75 0.2950851392636302 1107 76-77 0.4177287547915647 1107 78-79 0.5047955901617165 1107 80-81 0.5744473078282972 1107 82-83 0.497502929268979 1107 84-85 0.7125681523509719 1107 86-87 0.5912894003119185 1107 88-89 0.48037334337719884 1107 90-91 0.5889721126213345 1107 92-93 0.4758843239241308 1107 94-95 0.4122369680756961 1107 96-97 0.5360719766665305 1107 98-99 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-0.6664509765334756 2316 52-53 -0.6067947524665058 2316 54-55 -0.5712929989255855 2316 56-57 -0.3724091789677786 2316 58-59 -0.280549699750253 2316 60-61 -0.3738211105216678 2316 62-63 -0.2688607800122469 2316 64-65 -0.7449144463357484 2316 66-67 -0.43917368488251185 2316 68-69 -0.36024508627218665 2316 70-71 -0.46131227474869974 2316 72-73 -0.41780598979828554 2316 74-75 -0.8093004060085924 2316 76-77 -0.7834003674860632 2316 78-79 -0.8970593964372391 2316 80-81 -0.9518809901471315 2316 82-83 -0.9741379166220838 2316 84-85 -1.0618051878515438 2316 86-87 -1.4423734392374108 2316 88-89 -1.4264135033602479 2316 90-91 -1.2009795577559181 2316 92-93 -1.1066302270949002 2316 94-95 -1.3121262523454078 2316 96-97 -1.0842276915273033 2316 98-99 -1.1828963626472522 2316 100-101 -1.6052013991887577 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 8.0 5 15.0 6 46.0 7 98.0 8 253.0 9 430.0 10 821.0 11 1436.0 12 2257.0 13 3369.0 14 4234.0 15 6111.0 16 8812.0 17 13105.0 18 11186.0 19 5623.0 20 4642.0 21 4490.0 22 4736.0 23 5183.0 24 5958.0 25 6906.0 26 8292.0 27 10018.0 28 12620.0 29 15588.0 30 19521.0 31 24656.0 32 32024.0 33 42199.0 34 58990.0 35 87282.0 36 133434.0 37 209242.0 38 180827.0 39 20247.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.886603485017304 20.135867377848577 15.397773249335915 20.579755887798203 2 15.465336242313379 21.63569788527313 39.96386005138351 22.93510582102998 3 19.190482088283524 27.075744632201392 30.186807754316096 23.54696552519899 4 12.673752806562355 17.461184488403777 37.39531959083735 32.46974311419652 5 12.773587382793641 39.33157435562972 33.32313380371164 14.571704457865001 6 30.333527053620298 37.53653426996563 17.337012960204774 14.792925716209307 7 27.952037820974933 34.387891529342276 20.36561263776106 17.294458011921737 8 27.332768051184498 34.25525135471879 19.42379329727807 18.988187296818648 9 27.885029656141196 16.377727036471285 20.436325835405505 35.30091747198201 10-11 24.91639858107829 27.743550331812152 26.59091462439965 20.749136462709902 12-13 26.42854080422262 25.35912342073825 27.71211690546475 20.500218869574375 14-15 23.39828785140913 25.87817216969897 27.26454251842724 23.458997460464655 16-17 21.091375636339386 29.552188562881287 27.71877954102053 21.637656259758792 18-19 21.13895884343696 27.182237860989282 31.567144192466927 20.11165910310683 20-21 22.168746248654276 26.053420221645652 31.71830953114398 20.059523998556095 22-23 21.749939528219546 26.200980145672155 31.53234168434948 20.51673864175882 24-25 21.623717328836474 26.468584712269344 31.607843884489593 20.299854074404585 26-27 21.30058407721358 26.6219588684728 31.789361696496858 20.28809535781676 28-29 20.954238575523156 26.723443897197573 31.888813315301533 20.433504211977738 30-31 21.107466064545907 26.67618330808618 31.75297805244421 20.463372574923703 32-33 21.061153154412008 26.71815603692753 31.613086599319757 20.607604209340703 34-35 21.407679580293884 26.606475762204646 31.547189944329233 20.438654713172237 36-37 21.614102836890694 26.40878579723308 31.448794858684753 20.52831650719147 38-39 21.302509577509817 26.765686314984954 31.45879844727368 20.473005660231554 40-41 21.237353929081436 26.95861266634274 31.31975385879167 20.484279545784148 42-43 21.191252607171972 26.971392573927222 30.92550634720649 20.911848471694316 44-45 21.371529045869366 26.974756023589414 30.621513961092923 21.0322009694483 46-47 21.598488770045552 27.485150757785064 30.0077488114784 20.90861166069098 48-49 21.69598406200743 27.637745180546247 29.446383411615386 21.219887345830937 50-51 21.72652411817573 27.843692075781686 29.273411308395286 21.156372497647304 52-53 21.870914539713187 28.148563347063128 28.799590540945374 21.180931572278308 54-55 21.492789476859954 28.07366875524659 28.851143426054428 21.582398341839028 56-57 21.784375559062987 28.296076581969615 28.522348228623812 21.397199630343582 58-59 21.884305586871903 27.782770750565543 28.912488183591783 21.420435478970763 60-61 21.840956702986574 27.943516245510292 29.19285330928238 21.022673742220753 62-63 21.82618950078388 27.894556883368743 29.29320676941252 20.98604684643486 64-65 21.593301724110553 28.027990993594525 29.11954658867043 21.259160693624484 66-67 21.74949532160214 28.14152378472277 28.75793538634494 21.351045507330145 68-69 21.798190038542927 28.093146642022905 28.850878780853662 21.257784538580506 70-71 21.753253283453006 28.257491311698058 28.71601558654374 21.27323981830519 72-73 21.79100322285096 28.0139796254845 28.59117111385519 21.60384603780935 74-75 21.603834603101006 28.645725821220523 28.284538051216256 21.465901524462215 76-77 21.56577862323098 28.285226128738245 28.7248547362493 21.424140511781474 78-79 21.611985675284572 28.123210336829825 28.608199131752027 21.656604856133576 80-81 21.45305112259907 28.699834490803838 28.374532437844074 21.472581948753017 82-83 21.57411548630059 28.42823986471287 28.276491744362374 21.721152904624162 84-85 21.503020692298 28.27033475855104 28.100536600289416 22.126107948861538 86-87 21.37618680140283 28.15650270308608 28.334344278000255 22.132966217510834 88-89 21.481462662267205 27.847608824753006 28.448459288570188 22.2224692244096 90-91 21.288226712486846 27.823954383089728 28.638670621939056 22.24914828248437 92-93 21.374451055334045 27.764942383635905 28.705234247266326 22.155372313763724 94-95 21.067663426350826 27.665268281425824 28.668855811767397 22.598212480455953 96-97 21.149756367628175 27.637533464385637 28.55638160144221 22.656328566543976 98-99 21.065738501544736 27.6823024592459 28.407907822871625 22.84405121633774 100-101 20.879037111971147 27.83924551114386 28.54414374926882 22.73757362761617 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1085.0 1 768.0 2 579.5 3 754.5 4 638.5 5 574.0 6 1010.5 7 2115.0 8 3166.5 9 3427.5 10 2901.5 11 2295.5 12 2262.5 13 2362.5 14 2404.0 15 2430.5 16 2417.0 17 2478.0 18 2625.0 19 2678.5 20 2788.5 21 3078.5 22 3459.0 23 3872.5 24 4359.5 25 5101.5 26 6222.5 27 7351.5 28 8149.0 29 9619.0 30 11604.5 31 13076.5 32 15131.0 33 17680.0 34 20241.0 35 22405.0 36 25475.5 37 28511.0 38 29583.5 39 29841.0 40 29419.0 41 29617.5 42 29825.0 43 30687.0 44 32453.0 45 33641.0 46 33389.5 47 33411.0 48 37370.0 49 37054.0 50 33225.0 51 30637.5 52 27717.0 53 26876.0 54 26218.0 55 24586.5 56 22572.0 57 20652.0 58 18940.5 59 17062.0 60 14723.0 61 12339.5 62 9855.5 63 7129.5 64 5001.0 65 3707.5 66 2717.0 67 2175.0 68 1694.5 69 1144.0 70 774.0 71 543.5 72 433.5 73 349.0 74 254.0 75 194.0 76 122.5 77 63.5 78 37.0 79 24.0 80 15.5 81 9.5 82 8.0 83 4.0 84 2.5 85 1.0 86 1.0 87 1.0 88 0.5 89 0.0 90 0.0 91 0.0 92 0.5 93 0.5 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.053458330554558724 2 0.0 3 0.0 4 0.0 5 0.01831344789294784 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0036521037705589624 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 5.822194416833129E-4 24-25 1.587871204590853E-4 26-27 8.997936826014836E-4 28-29 0.0035462456902529056 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.015984570126214587 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 5.292904015302844E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 5.292904015302844E-5 82-83 3.705032810711991E-4 84-85 0.001058580803060569 86-87 0.0 88-89 0.0 90-91 0.010479949950299631 92-93 7.939356022954266E-4 94-95 0.0 96-97 0.0 98-99 1.587871204590853E-4 100-101 0.013496905239022253 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 944661.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 50.69858866759181 #Duplication Level Percentage of deduplicated Percentage of total 1 74.61532208617874 37.828915227470546 2 13.541706999458128 13.73090866045153 3 4.801771014144563 7.303290405662412 4 2.2000879908797693 4.461654243284876 5 1.2503767998456157 3.169616952743632 6 0.7407684960795526 2.25335503683887 7 0.5255556068630398 1.8651449274007093 8 0.36714601923062207 1.4891028007933649 9 0.27496648965681053 1.2546371660834046 >10 1.5036225709265372 14.01454949282382 >50 0.11870226842674199 4.117850201124516 >100 0.05663017913888319 5.0932360588461245 >500 0.0018807070337265514 0.6932945716199821 >1k 8.35869792767356E-4 0.629834386962224 >5k 6.26902344575517E-4 2.0946098678940506 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8248 0.8731174463643571 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6450 0.6827846179740669 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5073 0.5370180413926265 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1580 0.16725576688356988 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1554 0.1645034567956124 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1471 0.15571723613020966 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1340 0.14184982761011622 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 1.0585808030605688E-4 0.0 14-15 0.0 0.0 0.0 1.0585808030605688E-4 0.0 16-17 0.0 0.0 0.0 2.646452007651422E-4 0.0 18-19 0.0 0.0 0.0 8.46864642448455E-4 0.0 20-21 0.0 0.0 0.0 9.52722722754512E-4 0.0 22-23 0.0 0.0 0.0 0.0011644388833666258 0.0 24-25 0.0 0.0 0.0 0.0014820131242847963 0.0 26-27 0.0 0.0 0.0 0.0015349421644378248 0.0 28-29 0.0 0.0 0.0 0.002275948726580223 0.0 30-31 0.0 0.0 0.0 0.0034403876099468487 0.0 32-33 0.0 0.0 0.0 0.005028258814537702 0.0 34-35 0.0 0.0 0.0 0.006351484818363413 0.0 36-37 0.0 0.0 0.0 0.008733291625249693 0.0 38-39 0.0 0.0 0.0 0.010374091869993576 0.0 40-41 0.0 0.0 0.0 0.014079124680705565 0.0 42-43 0.0 0.0 0.0 0.020271822378609895 0.0 44-45 0.0 0.0 0.0 0.031228133690286777 0.0 46-47 0.0 0.0 0.0 0.05705750528496466 0.0 48-49 0.0 0.0 0.0 0.07690589534235033 0.0 50-51 0.0 0.0 0.0 0.10792231287202499 0.0 52-53 0.0 0.0 0.0 0.14619000890266456 0.0 54-55 0.0 0.0 0.0 0.1868395117401904 0.0 56-57 0.0 0.0 0.0 0.2368574546848023 0.0 58-59 0.0 0.0 0.0 0.30471248416098473 0.0 60-61 0.0 0.0 0.0 0.3891872322452181 0.0 62-63 0.0 0.0 0.0 0.4981151968801506 0.0 64-65 1.0585808030605688E-4 0.0 0.0 0.5875123456986157 0.0 66-67 1.0585808030605688E-4 0.0 0.0 0.7206288816834823 0.0 68-69 1.0585808030605688E-4 0.0 0.0 0.8892608036110308 0.0 70-71 1.0585808030605688E-4 0.0 0.0 1.02364763655957 0.0 72-73 1.0585808030605688E-4 0.0 0.0 1.2569059165139662 0.0 74-75 1.0585808030605688E-4 0.0 0.0 1.4489854032293066 0.0 76-77 1.0585808030605688E-4 0.0 0.0 1.619152267321293 0.0 78-79 1.0585808030605688E-4 0.0 0.0 1.8389665710768202 0.0 80-81 1.0585808030605688E-4 0.0 0.0 2.076988464644989 0.0 82-83 1.0585808030605688E-4 0.0 0.0 2.355183499689306 0.0 84-85 1.0585808030605688E-4 0.0 0.0 2.737807530955549 0.0 86-87 1.0585808030605688E-4 0.0 0.0 3.236822521518301 0.0 88-89 1.0585808030605688E-4 0.0 0.0 3.8347618881270638 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9265 0.0 27.85259 1 GGTATCA 6510 0.0 26.718454 1 GTACATG 7295 0.0 24.625084 1 TACCTGG 665 0.0 24.283737 2 TACATGG 7515 0.0 23.76387 2 ACATGGG 7405 0.0 23.47547 3 AGTACTT 6360 0.0 23.454317 12-13 CATGGGG 2020 0.0 23.277811 4 GAGTACT 6215 0.0 22.778513 12-13 ATCAACG 11400 0.0 22.539833 3 TCAACGC 11475 0.0 22.433907 4 TAAGGTG 320 0.0 22.263813 5 TATCAAC 11610 0.0 22.213953 2 CAACGCA 11605 0.0 22.1826 5 AACGCAG 11825 0.0 21.7699 6 GTACTTT 6710 0.0 21.058271 14-15 CATGGGA 5515 0.0 20.152485 4 AGGACCG 190 8.254494E-6 19.998375 5 ACGCAGA 12905 0.0 19.878609 7 TACTTTT 7445 0.0 19.840563 14-15 >>END_MODULE