FastQCFastQC Report
Thu 26 May 2016
SRR937364_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937364_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077236
Sequences flagged as poor quality0
Sequence length101
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84310.7826511553642841No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61620.5720195017619166No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47500.44094330304594354No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN16420.152427137600303No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA14860.1379456312265836No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT13930.12931242550378932No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13390.1242995963744249No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11490.10666186425258718No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA97000.031.7495371
GGTATCA63250.029.3799041
GTACATG80250.025.9988171
ATCAACG118450.025.835943
TCAACGC118500.025.8250374
TATCAAC119350.025.6411132
CAACGCA119550.025.635585
AACGCAG121850.025.3041366
GGACCGA1701.12071575E-725.1516176
TACATGG81800.024.9216882
GAGTACT64250.023.80643812-13
CGTCACT1801.9403706E-723.7631571
AGTACTT65500.023.67846912-13
ACATGGG81750.023.42563
GTCGGGA3950.022.8575652
ACGCAGA134400.022.7977717
CGCAGAG134450.022.7550168
GTACTTT66950.022.63297714-15
CATGGGG24300.022.0976284
TACCTGG6900.022.0381092
Produced by FastQC (version 0.11.5)