##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937364_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1077236 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.770613867341975 33.0 31.0 34.0 30.0 34.0 2 31.8479488245844 33.0 31.0 34.0 30.0 34.0 3 31.879243731178683 33.0 31.0 34.0 30.0 34.0 4 35.50833429257841 37.0 35.0 37.0 33.0 37.0 5 35.53175441593114 37.0 35.0 37.0 33.0 37.0 6 35.56956507209191 37.0 35.0 37.0 33.0 37.0 7 35.46089157807574 37.0 35.0 37.0 33.0 37.0 8 35.472353319049866 37.0 35.0 37.0 33.0 37.0 9 37.08434456330832 39.0 37.0 39.0 33.0 39.0 10-11 36.97476551099295 39.0 37.0 39.0 33.0 39.0 12-13 36.82702258372353 39.0 37.0 39.0 33.0 39.0 14-15 37.95829883145383 40.0 38.0 41.0 33.0 41.0 16-17 37.9140986747565 40.0 38.0 41.0 33.0 41.0 18-19 37.92652399288549 40.0 38.0 41.0 33.0 41.0 20-21 37.83962659992797 40.0 38.0 41.0 33.0 41.0 22-23 37.69071633328259 40.0 37.0 41.0 32.5 41.0 24-25 37.61936938609553 40.0 37.0 41.0 32.0 41.0 26-27 37.6153178133668 40.0 37.0 41.0 32.0 41.0 28-29 37.5252145305207 40.0 37.0 41.0 32.0 41.0 30-31 37.26657529083692 39.5 36.5 41.0 32.0 41.0 32-33 36.996871623302596 39.0 36.0 41.0 31.0 41.0 34-35 36.98762156110639 39.0 36.0 41.0 31.0 41.0 36-37 36.83710626083792 39.0 35.5 41.0 31.0 41.0 38-39 36.70394788142988 39.0 35.0 41.0 31.0 41.0 40-41 36.45292721372104 39.0 35.0 41.0 30.0 41.0 42-43 36.139110185697476 39.0 35.0 41.0 30.0 41.0 44-45 35.81410248079344 39.0 35.0 41.0 28.5 41.0 46-47 35.358184743176054 39.0 35.0 40.5 26.5 41.0 48-49 34.946394754724125 39.0 35.0 40.0 24.0 41.0 50-51 33.95017758411342 38.0 34.0 39.5 23.5 40.5 52-53 34.05362845281814 38.0 33.5 39.5 23.0 40.5 54-55 34.42035310739708 38.0 34.0 40.0 23.0 41.0 56-57 34.209188144473444 38.0 34.0 40.0 22.5 41.0 58-59 33.94157222744134 38.0 34.0 40.0 21.5 41.0 60-61 33.62390878136267 37.5 33.5 40.0 20.0 41.0 62-63 33.298921963246684 37.0 33.0 40.0 18.5 41.0 64-65 32.87407169830938 36.5 32.5 39.5 16.5 41.0 66-67 32.51061559398312 36.0 32.0 39.0 13.0 41.0 68-69 32.03147360466973 35.5 31.5 39.0 8.0 40.0 70-71 31.571859369720283 35.0 31.0 38.5 7.0 40.0 72-73 31.111645451878694 35.0 31.0 37.5 4.0 40.0 74-75 30.62332812865519 35.0 30.0 37.0 2.0 39.0 76-77 30.144228841219565 34.5 30.0 36.5 2.0 39.0 78-79 29.649878021157853 34.0 29.0 36.0 2.0 38.5 80-81 29.163653089945008 34.0 29.0 35.5 2.0 37.0 82-83 28.685693292834625 34.0 28.5 35.0 2.0 37.0 84-85 28.16076050187703 34.0 27.5 35.0 2.0 36.0 86-87 27.709736770772608 33.5 26.5 35.0 2.0 36.0 88-89 27.244643699245106 33.0 26.0 35.0 2.0 35.5 90-91 26.742525778937946 33.0 25.0 35.0 2.0 35.0 92-93 26.24168705836047 33.0 24.5 35.0 2.0 35.0 94-95 25.699820652113374 32.0 22.5 35.0 2.0 35.0 96-97 24.96484196592019 32.0 18.5 34.0 2.0 35.0 98-99 24.067948898848535 31.5 2.0 34.0 2.0 35.0 100-101 21.58491361224467 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.038696228967459945 1101 2 0.03522812324349189 1101 3 -0.009685318508125818 1101 4 -0.11681059573859898 1101 5 -0.03110272571467476 1101 6 -0.022476526391649543 1101 7 0.13237517338459526 1101 8 -0.24567324036214444 1101 9 -0.14904248683670573 1101 10-11 -0.20402449974937298 1101 12-13 -0.15585906871078237 1101 14-15 -0.1150550433802735 1101 16-17 -0.18396624467602862 1101 18-19 -0.09606275610146042 1101 20-21 0.03431690667960652 1101 22-23 -0.11185937818849823 1101 24-25 -0.30357966138856796 1101 26-27 -0.27294035728028376 1101 28-29 -0.16289577326029558 1101 30-31 0.02203749889230977 1101 32-33 -0.2420425405673683 1101 34-35 -0.08400887971669846 1101 36-37 -0.08261225177002984 1101 38-39 -0.3611954935794799 1101 40-41 -0.2988305569075109 1101 42-43 -0.28777812867250674 1101 44-45 -0.08033402916436927 1101 46-47 -0.09727161054635758 1101 48-49 -0.11117020371693087 1101 50-51 -0.2706305227987187 1101 52-53 -0.14771929856148347 1101 54-55 -0.08658196738444701 1101 56-57 -0.1782993127465602 1101 58-59 -0.30207283025562504 1101 60-61 -0.19371521600576358 1101 62-63 -0.3876061477697377 1101 64-65 -0.24466797776077698 1101 66-67 -0.36271458072623375 1101 68-69 -0.5052091133548515 1101 70-71 -0.3810498616545779 1101 72-73 -0.23843509382763983 1101 74-75 -0.09365775629018813 1101 76-77 -0.1239751522435597 1101 78-79 -0.19977771249054044 1101 80-81 -0.28241295080475837 1101 82-83 -0.21765330100588542 1101 84-85 -0.3659314124475159 1101 86-87 -0.35224552069349 1101 88-89 -0.43572748011724727 1101 90-91 -0.3653521127035759 1101 92-93 -0.19700880326452008 1101 94-95 -0.09842652796436724 1101 96-97 0.20529807616561513 1101 98-99 0.329440864506914 1101 100-101 0.5864109046471278 1102 1 -0.08897385189382234 1102 2 -0.12302737842003708 1102 3 -0.12374161060098743 1102 4 -0.15248622807915524 1102 5 -0.10502291477635595 1102 6 -0.04773471142507901 1102 7 -0.05748007336169536 1102 8 -0.1691521751241325 1102 9 -0.10408166264303986 1102 10-11 -0.1713665692846078 1102 12-13 -0.08880125396961347 1102 14-15 0.029025479792622377 1102 16-17 -0.033212734190328774 1102 18-19 -0.11414108215269181 1102 20-21 -0.08628340365947196 1102 22-23 -0.06976579195961108 1102 24-25 0.017263424504250224 1102 26-27 -0.16236100150981514 1102 28-29 -0.06413829145684957 1102 30-31 -0.10491047138439313 1102 32-33 -0.1427317117552107 1102 34-35 0.21846482831243463 1102 36-37 0.1903949292508429 1102 38-39 0.17445893438751625 1102 40-41 0.15712737826287793 1102 42-43 0.060499930958840764 1102 44-45 0.050708708976721084 1102 46-47 0.10192634128544142 1102 48-49 0.016354060767248768 1102 50-51 -0.08196620662234722 1102 52-53 -0.024516950162592366 1102 54-55 0.03748598420916238 1102 56-57 -0.016442843698847298 1102 58-59 -0.26870295568675573 1102 60-61 -0.1315082648054542 1102 62-63 0.013164645732778979 1102 64-65 -0.12981753830739962 1102 66-67 -0.10662006615243769 1102 68-69 -0.18153626023958225 1102 70-71 0.019293939667992532 1102 72-73 0.07750571518021232 1102 74-75 -0.08029464388318885 1102 76-77 0.11178640138055584 1102 78-79 -0.2053672434556013 1102 80-81 -0.10550863829993062 1102 82-83 -0.1920398700975774 1102 84-85 0.008751901685691621 1102 86-87 0.050878667803516464 1102 88-89 -0.17078987035556636 1102 90-91 -0.1816112777857235 1102 92-93 -0.08739497266829588 1102 94-95 0.04385714240232019 1102 96-97 0.15590611883927252 1102 98-99 -0.13497442053505893 1102 100-101 0.4913929448331942 1103 1 -0.038179746371767465 1103 2 -0.09804082895351485 1103 3 -0.2324423007123393 1103 4 -0.2939794352686178 1103 5 -0.1957924716191286 1103 6 -0.1243526766796137 1103 7 0.022222198206321764 1103 8 0.00951923824162293 1103 9 -0.21865284100293536 1103 10-11 -0.138710886638016 1103 12-13 -0.20923855352173604 1103 14-15 -0.46923439383059673 1103 16-17 -0.41688599918796143 1103 18-19 -0.4488110258584257 1103 20-21 -0.4918510511087604 1103 22-23 -0.4827717008779544 1103 24-25 -0.43576655562855393 1103 26-27 -0.46123243770071554 1103 28-29 -0.41232456562413944 1103 30-31 -0.39231722017500914 1103 32-33 -0.40533969450126506 1103 34-35 -0.355967570820205 1103 36-37 -0.47457528160228435 1103 38-39 -0.7208831051093014 1103 40-41 -0.565657126722563 1103 42-43 -0.5381096565348358 1103 44-45 -0.8237122193546469 1103 46-47 -0.853632746733453 1103 48-49 -0.8043346065469095 1103 50-51 -0.8238219377141505 1103 52-53 -0.6797506633715926 1103 54-55 -0.6573784740080058 1103 56-57 -0.6293897682431009 1103 58-59 -0.6642455886374208 1103 60-61 -0.4706774410738248 1103 62-63 -0.7571153815642333 1103 64-65 -0.710033375699183 1103 66-67 -0.6445403807668235 1103 68-69 -0.45969969761366514 1103 70-71 -0.525527779624614 1103 72-73 -0.5591502492870895 1103 74-75 -0.5478386812791669 1103 76-77 -0.7207506720162691 1103 78-79 -0.7611243629219615 1103 80-81 -0.893585002393646 1103 82-83 -0.7613808638299773 1103 84-85 -0.49680969011215836 1103 86-87 -0.38814237791432404 1103 88-89 -0.4036515033784305 1103 90-91 -0.5426285457087054 1103 92-93 -0.7248256529275672 1103 94-95 -0.9460776661808659 1103 96-97 -0.5706748137776323 1103 98-99 -0.19642618144862212 1103 100-101 0.11843527150763933 1104 1 -0.10375113138798042 1104 2 -0.09440297868050251 1104 3 -0.07117582067316874 1104 4 -0.19949464725711863 1104 5 -0.049132826580922995 1104 6 -0.1052302878476965 1104 7 -0.2659024726548438 1104 8 -0.20898968199821866 1104 9 -0.14510128284814527 1104 10-11 -0.13720493211229012 1104 12-13 -0.09992634974668135 1104 14-15 -0.18329127826012837 1104 16-17 -0.3343772452498399 1104 18-19 -0.16614544015298094 1104 20-21 -0.20096507865940794 1104 22-23 -0.3188097470174398 1104 24-25 -0.1500786670137515 1104 26-27 -0.23877154170396864 1104 28-29 -0.22664522307029955 1104 30-31 -0.2551055919887162 1104 32-33 -0.22273115952708622 1104 34-35 0.040962314523987686 1104 36-37 -0.02371513211551246 1104 38-39 0.10673707098575136 1104 40-41 0.01942699423423022 1104 42-43 0.013867717191338613 1104 44-45 0.02173111333195976 1104 46-47 0.02588165222439187 1104 48-49 0.3131922096065338 1104 50-51 0.008187911043670226 1104 52-53 -0.05578542967147371 1104 54-55 -0.1522104168741194 1104 56-57 -0.019602911622904173 1104 58-59 0.17095979399513084 1104 60-61 0.06961954609322873 1104 62-63 0.36607839690339006 1104 64-65 0.5066092696069475 1104 66-67 0.2429414877254743 1104 68-69 0.09138149458493672 1104 70-71 0.03717836522418949 1104 72-73 0.08002928266594012 1104 74-75 -0.04849349432830863 1104 76-77 -0.06856957867480418 1104 78-79 -0.018096331842016866 1104 80-81 0.02105370710495791 1104 82-83 0.0016426401474056718 1104 84-85 0.10271265437262755 1104 86-87 0.0891920825751562 1104 88-89 0.13969550365243677 1104 90-91 0.11171447008072022 1104 92-93 0.2742897142758842 1104 94-95 0.2235674182172609 1104 96-97 0.20394939051480065 1104 98-99 0.4136159021272334 1104 100-101 0.720337809456769 1105 1 0.0031961189966125403 1105 2 -0.15322037530349064 1105 3 -0.12843862348302082 1105 4 -0.0409307119801241 1105 5 -0.13102586311271835 1105 6 -0.046192792249676984 1105 7 -0.014496156045943565 1105 8 -0.09778409565455348 1105 9 -0.13532888835664636 1105 10-11 -0.1759029655858697 1105 12-13 -0.11169816966401669 1105 14-15 -0.123967653994562 1105 16-17 -0.23077434087010573 1105 18-19 -0.09331816456126063 1105 20-21 -0.1696948063853938 1105 22-23 -0.1350120434606481 1105 24-25 -0.10184223901161005 1105 26-27 0.02102633793200681 1105 28-29 -0.12998933739202556 1105 30-31 -0.1413908773296555 1105 32-33 -0.1288392536089802 1105 34-35 -0.03494287147123032 1105 36-37 -0.02517705267599979 1105 38-39 0.12300701890337962 1105 40-41 0.07206308490574287 1105 42-43 -0.061176765969939595 1105 44-45 0.18005178132076338 1105 46-47 0.16353039279488968 1105 48-49 0.11847875530371965 1105 50-51 -0.18321247447706668 1105 52-53 -0.07941131701787185 1105 54-55 -0.0631189854430616 1105 56-57 0.08430697290621936 1105 58-59 0.04965166469741433 1105 60-61 0.23372186663147687 1105 62-63 0.18851181117136662 1105 64-65 0.18148601418093335 1105 66-67 -0.03727925996233239 1105 68-69 -0.06612828244321634 1105 70-71 -0.15925533633086886 1105 72-73 -0.0031252685174969486 1105 74-75 0.033903453014751506 1105 76-77 -0.04628513666045819 1105 78-79 0.02755103716433993 1105 80-81 -9.434478261844959E-4 1105 82-83 0.1481775872309754 1105 84-85 0.22409376385680702 1105 86-87 0.2397351478356633 1105 88-89 0.42641148793505224 1105 90-91 0.8302912869398362 1105 92-93 0.9661580997224739 1105 94-95 0.7279809818745164 1105 96-97 0.7054750239364829 1105 98-99 0.9270025534719224 1105 100-101 1.2531325567368086 1106 1 0.0276359696234465 1106 2 0.07044297195948346 1106 3 0.04794668420396775 1106 4 0.19563113661160259 1106 5 0.18586481390732246 1106 6 0.10083900882320052 1106 7 -0.17587128817994113 1106 8 -0.0828628327004779 1106 9 -0.1330665566731355 1106 10-11 -0.46779685333866183 1106 12-13 -0.024915768431093 1106 14-15 -0.1622624338370926 1106 16-17 0.1262930993361735 1106 18-19 -0.20160136106798632 1106 20-21 -0.15266927859033075 1106 22-23 -0.03981937479838393 1106 24-25 -0.2558425650244729 1106 26-27 -0.39176398640139354 1106 28-29 -0.05042861502655427 1106 30-31 -0.17196199259002043 1106 32-33 0.015097895062773148 1106 34-35 0.10833792933491537 1106 36-37 -0.0989695701868385 1106 38-39 0.20374645065307817 1106 40-41 0.21790814976254325 1106 42-43 0.44613999998247067 1106 44-45 0.5832815314763593 1106 46-47 0.6536394836141639 1106 48-49 0.8182517811970413 1106 50-51 0.5492482804896923 1106 52-53 0.5558784639607595 1106 54-55 0.572965451888372 1106 56-57 0.6332101372068237 1106 58-59 0.587133882177632 1106 60-61 0.4700274740527206 1106 62-63 0.4257466787409854 1106 64-65 0.5624555072803261 1106 66-67 0.6530282788381356 1106 68-69 0.392218634759395 1106 70-71 0.5718698773063515 1106 72-73 0.4943388700676046 1106 74-75 0.7488712715935293 1106 76-77 0.6651595201863039 1106 78-79 0.4281992347083765 1106 80-81 0.4450427657813911 1106 82-83 0.6249392915867062 1106 84-85 0.6350008699492946 1106 86-87 0.5310708620508144 1106 88-89 0.8947666295530645 1106 90-91 0.8844995696423901 1106 92-93 1.1042455403239195 1106 94-95 1.047115467119962 1106 96-97 1.3849531316787953 1106 98-99 1.6376661378351614 1106 100-101 1.9973779357514587 1107 1 -0.021384311080716145 1107 2 -0.12943768492333518 1107 3 -0.09363949893878498 1107 4 -0.10692400079820885 1107 5 0.047238695939384456 1107 6 -0.1048696338299493 1107 7 0.023581690758980756 1107 8 -0.13706036780121167 1107 9 -0.1519930532599716 1107 10-11 -0.18812629880621756 1107 12-13 0.03630191265206406 1107 14-15 6.434630346987547E-4 1107 16-17 -0.03926687915918592 1107 18-19 -0.04498893896192868 1107 20-21 -0.04961319240909745 1107 22-23 -0.12363154220410877 1107 24-25 -0.08682621158582293 1107 26-27 -0.11366274937629584 1107 28-29 0.0480682209192409 1107 30-31 0.10926644081232695 1107 32-33 0.11455367011306805 1107 34-35 0.08074602941536568 1107 36-37 0.018740999241245504 1107 38-39 0.027191399668531346 1107 40-41 0.10977171986295531 1107 42-43 -0.0011641038193559439 1107 44-45 0.08675315793622218 1107 46-47 0.049901209806229474 1107 48-49 0.30306349487015183 1107 50-51 0.25836913223604796 1107 52-53 0.1373453929535131 1107 54-55 0.25214980078207105 1107 56-57 0.24168019403143148 1107 58-59 0.28204540431710967 1107 60-61 0.5530014435268029 1107 62-63 0.5305744827640808 1107 64-65 0.6380709377480329 1107 66-67 0.444273463489516 1107 68-69 0.5061452393778083 1107 70-71 0.6522326600472219 1107 72-73 0.6962403528963073 1107 74-75 0.5302515846817464 1107 76-77 0.4386071382171579 1107 78-79 0.6995586659284854 1107 80-81 0.8331687611017209 1107 82-83 0.7927101045052503 1107 84-85 0.9015490831705826 1107 86-87 0.6721070373771632 1107 88-89 0.6629404002490524 1107 90-91 0.8208848585378838 1107 92-93 0.7980147874200973 1107 94-95 0.8376381111737139 1107 96-97 0.9395017694006249 1107 98-99 1.2075781365845373 1107 100-101 1.6966625125282278 1108 1 -0.09057478481242143 1108 2 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0.2833464356389257 2315 56-57 0.26910898071436407 2315 58-59 0.22684073329752863 2315 60-61 -0.015079025727693818 2315 62-63 0.1627379684496688 2315 64-65 -0.116945385707524 2315 66-67 0.2394706006065448 2315 68-69 0.27579168409331345 2315 70-71 0.06283752479573579 2315 72-73 0.0960242016442443 2315 74-75 0.24286190254068174 2315 76-77 0.08643934744439008 2315 78-79 -0.13294115469288315 2315 80-81 0.024586390433711358 2315 82-83 0.13733681757118887 2315 84-85 0.13866431600404638 2315 86-87 -0.03871577240973423 2315 88-89 0.17763151024403 2315 90-91 0.12527873813894885 2315 92-93 0.09401643770351242 2315 94-95 0.33921215867317756 2315 96-97 0.21925081869386887 2315 98-99 0.6487607799080628 2315 100-101 0.8968930140708835 2316 1 0.013314390255651176 2316 2 0.263850861190722 2316 3 0.05724081229302769 2316 4 0.03169057845673251 2316 5 0.04134302405859103 2316 6 0.2065486212086114 2316 7 0.29293061882805915 2316 8 -0.11326845228788329 2316 9 -0.09551599686992063 2316 10-11 -0.22537923887379918 2316 12-13 0.013921768181177185 2316 14-15 0.15884778970380609 2316 16-17 0.22468976714399957 2316 18-19 0.46858705740869055 2316 20-21 0.42517379899598495 2316 22-23 0.47336738265403255 2316 24-25 0.4322684319704777 2316 26-27 0.2594145800459202 2316 28-29 0.22585297296335938 2316 30-31 0.1820073481989013 2316 32-33 0.5329443961341909 2316 34-35 0.2380119374751999 2316 36-37 0.1859721671684582 2316 38-39 0.31430917016820814 2316 40-41 0.13597907930215314 2316 42-43 0.2596551584137501 2316 44-45 -0.22072394609981671 2316 46-47 -0.03641465816883027 2316 48-49 -0.03453518927222632 2316 50-51 -0.32458044381831996 2316 52-53 -0.4786321678624361 2316 54-55 -0.6379402501162659 2316 56-57 -0.4359315823970604 2316 58-59 -0.12309663248630187 2316 60-61 -0.3948779997195899 2316 62-63 -0.5387980611375411 2316 64-65 -0.527769174779344 2316 66-67 -0.45910670791170816 2316 68-69 -0.22264246050224656 2316 70-71 -0.1367091227824062 2316 72-73 -0.22027560747052632 2316 74-75 -0.4867849597833427 2316 76-77 -0.3707934132332511 2316 78-79 -0.7675987543111127 2316 80-81 -0.5791149934842075 2316 82-83 -0.4897062509088883 2316 84-85 -0.47098135328729285 2316 86-87 -0.34950973566433063 2316 88-89 -0.6505121308917374 2316 90-91 -0.8418561938581881 2316 92-93 -0.7670250798340668 2316 94-95 -0.7626877458842074 2316 96-97 -0.805167577870197 2316 98-99 -0.6195612196147238 2316 100-101 -0.10587183918609355 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4054.0 3 938.0 4 666.0 5 755.0 6 860.0 7 1080.0 8 1349.0 9 1723.0 10 2219.0 11 2945.0 12 4353.0 13 5933.0 14 6455.0 15 8634.0 16 11561.0 17 15916.0 18 12674.0 19 7612.0 20 7479.0 21 8014.0 22 8410.0 23 9429.0 24 10608.0 25 11910.0 26 13570.0 27 16083.0 28 18758.0 29 22417.0 30 27557.0 31 33610.0 32 42666.0 33 55660.0 34 74458.0 35 105258.0 36 149147.0 37 200250.0 38 153746.0 39 18478.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.97985636958535 19.487193807974606 15.04349404487636 21.48945577756368 2 14.863374757480308 20.87311741907868 40.98961992453392 23.27388789890709 3 18.542916344506814 26.938797536605268 30.122713828077824 24.395572290810094 4 11.925683597021091 16.85061539549442 38.04212304932469 33.18157795815981 5 12.660975755004205 38.81888459620209 33.636581879899055 14.883557768894645 6 29.953916621756083 37.28751243767202 17.84611632818514 14.912454612386759 7 27.579081597036385 34.09409278969438 20.835191019811703 17.49163459345753 8 26.730611906807226 34.30845791517794 19.65450584060287 19.30642433741197 9 27.421321138551853 16.36170406809125 20.510665780721006 35.7063090126359 10-11 24.474648454914092 27.518192673417897 27.047635534137264 20.959523337530747 12-13 26.156013279389306 25.193378547289907 27.8294787923462 20.821129380974586 14-15 23.06932462072322 25.778816706696656 27.23204024526084 23.91981842731928 16-17 20.877928331868226 29.44188988015601 27.67319860559725 22.006983182378516 18-19 20.91488518219347 27.277837129573452 31.313071622934523 20.494206065298556 20-21 22.12156543812764 26.029019302777428 31.326954285246366 20.52246097384857 22-23 21.61790958945792 26.189336299840555 31.20262184151426 20.990132269187267 24-25 21.24685615495771 26.55549962362255 31.311487002649063 20.886157218770673 26-27 21.03966269540319 26.631876624385725 31.372593722345314 20.955866957865773 28-29 20.697413817976045 26.643808268671805 31.717330221017253 20.941447692334897 30-31 20.9087262326803 26.589521670620712 31.459419765139927 21.042332331559056 32-33 20.722028347084017 26.71454625073818 31.339177974584697 21.224247427593106 34-35 21.232062899749568 26.63791735332568 31.11678795768024 21.01323178924451 36-37 21.300092494198175 26.43500933316708 31.025708548362186 21.239189624272562 38-39 21.03535086833673 26.750317190540905 31.10947133048812 21.104860610634248 40-41 20.963229211929594 26.845075874235512 31.046607594960264 21.145087318874626 42-43 20.91127006829245 26.897397554350604 30.61695837594438 21.574374001412565 44-45 21.047769737301348 26.96409707517975 30.28806105591363 21.70007213160528 46-47 21.41368909804301 27.291029574717708 29.778556107449827 21.51672521978945 48-49 21.295930002559746 27.5175109952761 29.238964000651574 21.947595001512575 50-51 21.3671828458239 27.719807428809723 29.084225694030742 21.828784031335637 52-53 21.52207755448627 27.94586194824735 28.776330686608965 21.75572981065741 54-55 21.178863907572644 27.833249869218868 28.82411473740452 22.163771485803966 56-57 21.425670290773763 27.99856518298337 28.536481990580643 22.039282535662228 58-59 21.547959217373226 27.5326454982401 28.87051436922892 22.048880915157753 60-61 21.56920293309829 27.689406008060676 29.113768026623937 21.6276230322171 62-63 21.57688199853377 27.659210247211753 29.177443183721163 21.586464570533312 64-65 21.43727615237918 27.733475975626774 29.107586399367413 21.721661472626632 66-67 21.4455989807789 27.872524800256016 28.721410573721194 21.96046564524389 68-69 21.35958232821136 27.811930476104536 28.925557557788505 21.902929637895603 70-71 21.43195379671821 27.935151297513634 28.7809958489239 21.85189905684425 72-73 21.534408787720487 27.803065226421776 28.556881873837938 22.105644112019807 74-75 21.193284980803664 28.35168301134754 28.453793654625986 22.001238353222814 76-77 21.222465766426787 28.04312515964656 28.66435674475022 22.070052329176434 78-79 21.25469776582213 27.843061356845965 28.66890974613688 22.233331131195026 80-81 21.213700136681297 28.329873655866457 28.38607209217288 22.07035411527937 82-83 21.30717671209745 28.069183059909747 28.371315852272648 22.252324375720153 84-85 21.24063079578868 27.885189249435626 28.181366116380254 22.692813838395445 86-87 21.169265639545515 27.872024370284663 28.39149328937899 22.567216700790834 88-89 21.249199839223508 27.513063089885968 28.45943929198797 22.77829777890255 90-91 21.112258760910425 27.49781326186887 28.69619228407124 22.69373569314946 92-93 21.18207213849922 27.510647255438762 28.719328810042725 22.587951796019286 94-95 20.82317088771589 27.249061829691446 28.856848724660562 23.070918557932103 96-97 20.88132133065415 27.223669390050944 28.848488119292327 23.046521160002587 98-99 20.8762049574595 27.21484650896175 28.557829117746408 23.351119415832343 100-101 21.023180785567966 26.89196254309535 29.0635680808825 23.021288590454184 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2039.0 1 1302.0 2 630.5 3 708.5 4 625.0 5 617.5 6 993.5 7 2071.5 8 3200.0 9 3519.0 10 3035.5 11 2477.5 12 2404.0 13 2461.5 14 2471.5 15 2509.0 16 2535.5 17 2567.0 18 2663.0 19 2746.5 20 3000.0 21 3268.0 22 3549.5 23 4071.0 24 4825.0 25 5792.0 26 7048.0 27 8422.0 28 9453.0 29 10828.5 30 13005.5 31 15206.5 32 17599.0 33 20408.5 34 23458.5 35 25755.0 36 29063.5 37 32555.0 38 33511.5 39 33762.5 40 33841.5 41 34350.0 42 34571.0 43 35357.0 44 37153.0 45 38463.5 46 38364.5 47 38295.5 48 41782.5 49 41794.5 50 37978.0 51 34676.0 52 31561.5 53 30617.0 54 29989.0 55 28290.5 56 26016.5 57 23751.5 58 21723.5 59 19424.0 60 16827.0 61 14272.5 62 11441.5 63 8367.0 64 5943.0 65 4452.5 66 3265.0 67 2523.0 68 1989.0 69 1448.5 70 993.0 71 680.0 72 513.5 73 408.0 74 303.5 75 220.0 76 144.0 77 92.0 78 64.0 79 36.5 80 23.5 81 19.0 82 13.0 83 10.0 84 9.0 85 5.5 86 4.5 87 5.0 88 5.0 89 7.0 90 5.0 91 1.0 92 1.0 93 1.0 94 0.5 95 0.5 96 0.0 97 0.0 98 0.5 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.2739418289028588 2 0.23959466634980636 3 0.22826938572420527 4 0.23021881927451368 5 0.2357886294182519 6 0.2672580567303729 7 0.2665154153778745 8 0.2576037191478933 9 0.2485991927488498 10-11 0.2566754174572703 12-13 0.2589033415147656 14-15 0.24367919378854774 16-17 0.25969239795179516 18-19 0.24729957038197758 20-21 0.23875919482824562 22-23 0.23871277974371446 24-25 0.2336535355298189 26-27 0.24126560939292785 28-29 0.2594603225291394 30-31 0.2607599448960116 32-33 0.26034220913523126 34-35 0.2684184338436517 36-37 0.23894485516637023 38-39 0.23824862889840295 40-41 0.23179693214857283 42-43 0.240708628378554 44-45 0.26094560523413624 46-47 0.2650765477574088 48-49 0.27022862214036664 50-51 0.27217805569067505 52-53 0.2974278616756217 54-55 0.27124975400005197 56-57 0.23527806348840924 58-59 0.2151339168018893 60-61 0.21012108767252488 62-63 0.2198218403395356 64-65 0.2122097664764267 66-67 0.2132773134206432 68-69 0.21583014306985654 70-71 0.2166656145914173 72-73 0.220239576100316 74-75 0.2247882543843689 76-77 0.24103353397027208 78-79 0.23421051654419273 80-81 0.22956900809107755 82-83 0.2207037269456275 84-85 0.22014674593125366 86-87 0.230636555035294 88-89 0.22761957454076917 90-91 0.23913051550449485 92-93 0.22543806556780505 94-95 0.2232101415103097 96-97 0.2349995729812223 98-99 0.24256523175980008 100-101 0.21295240782892513 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1077236.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.151225570501694 #Duplication Level Percentage of deduplicated Percentage of total 1 74.96815228493729 38.347128681305485 2 13.29772815058046 13.603901844111027 3 4.714332655791144 7.2343167927226535 4 2.2584707445705527 4.62094185999629 5 1.2293206229009213 3.1440628240237336 6 0.7682175828186009 2.3577162519587893 7 0.5060781587815883 1.8120562639298838 8 0.34533908524471235 1.4131613958130396 9 0.25554730855302393 1.1764402221357293 >10 1.4792923798645197 13.908110240372817 >50 0.11636227928022988 4.1042112148849075 >100 0.057529148061535036 5.110434147637182 >500 0.002177759169258245 0.7212829520195958 >1k 0.0010888795846291225 1.0915786657398234 >5k 3.6295986154304084E-4 1.3546566433490297 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8431 0.7826511553642841 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6162 0.5720195017619166 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4750 0.44094330304594354 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 1642 0.152427137600303 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1486 0.1379456312265836 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1393 0.12931242550378932 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1339 0.1242995963744249 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1149 0.10666186425258718 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.641508453115195E-5 0.0 12-13 0.0 0.0 0.0 9.28301690623039E-5 0.0 14-15 0.0 0.0 0.0 9.28301690623039E-5 0.0 16-17 0.0 0.0 0.0 1.3924525359345586E-4 0.0 18-19 0.0 0.0 0.0 2.7849050718691166E-4 0.0 20-21 0.0 0.0 0.0 3.2490559171806366E-4 0.0 22-23 0.0 0.0 0.0 4.1773576078036755E-4 0.0 24-25 0.0 0.0 0.0 5.569810143738234E-4 0.0 26-27 0.0 0.0 0.0 0.0012532072823411026 0.0 28-29 0.0 0.0 0.0 0.0018566033812460777 0.0 30-31 0.0 0.0 0.0 0.0035739615088986997 0.0 32-33 0.0 0.0 0.0 0.004966414044833259 0.0 34-35 0.0 0.0 0.0 0.006312451496236665 0.0 36-37 0.0 0.0 0.0 0.008261885046545047 0.0 38-39 0.0 0.0 0.0 0.010304148765915733 0.0 40-41 0.0 0.0 0.0 0.014435091289188256 0.0 42-43 0.0 0.0 0.0 0.02056188244730031 0.0 44-45 0.0 0.0 0.0 0.03365093628508516 0.0 46-47 0.0 0.0 0.0 0.05931847803081219 0.0 48-49 0.0 0.0 0.0 0.08206186945107664 0.0 50-51 0.0 0.0 0.0 0.11432035320022724 0.0 52-53 0.0 0.0 0.0 0.15484072199592291 0.0 54-55 0.0 0.0 0.0 0.19782109027176958 0.0 56-57 0.0 0.0 0.0 0.2468818346211972 0.0 58-59 0.0 0.0 0.0 0.31873238547542043 0.0 60-61 0.0 0.0 0.0 0.4095667059028848 0.0 62-63 0.0 0.0 0.0 0.5153002684648489 0.0 64-65 0.0 0.0 0.0 0.6043708156801295 0.0 66-67 0.0 0.0 0.0 0.7389745608204702 0.0 68-69 0.0 0.0 0.0 0.8999420739745051 0.0 70-71 0.0 0.0 0.0 1.035845441481718 0.0 72-73 0.0 0.0 0.0 1.250932943199076 0.0 74-75 0.0 0.0 0.0 1.4357578098021233 0.0 76-77 0.0 0.0 0.0 1.6032698498750506 0.0 78-79 0.0 0.0 0.0 1.8177539554935038 0.0 80-81 0.0 0.0 0.0 2.054888622363159 0.0 82-83 0.0 0.0 0.0 2.3214504528255646 0.0 84-85 0.0 0.0 0.0 2.67100245442967 0.0 86-87 0.0 0.0 0.0 3.1160302849143546 0.0 88-89 0.0 4.641508453115195E-5 0.0 3.6300309310123318 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9700 0.0 31.749537 1 GGTATCA 6325 0.0 29.379904 1 GTACATG 8025 0.0 25.998817 1 ATCAACG 11845 0.0 25.83594 3 TCAACGC 11850 0.0 25.825037 4 TATCAAC 11935 0.0 25.641113 2 CAACGCA 11955 0.0 25.63558 5 AACGCAG 12185 0.0 25.304136 6 GGACCGA 170 1.12071575E-7 25.151617 6 TACATGG 8180 0.0 24.921688 2 GAGTACT 6425 0.0 23.806438 12-13 CGTCACT 180 1.9403706E-7 23.763157 1 AGTACTT 6550 0.0 23.678469 12-13 ACATGGG 8175 0.0 23.4256 3 GTCGGGA 395 0.0 22.857565 2 ACGCAGA 13440 0.0 22.797771 7 CGCAGAG 13445 0.0 22.755016 8 GTACTTT 6695 0.0 22.632977 14-15 CATGGGG 2430 0.0 22.097628 4 TACCTGG 690 0.0 22.038109 2 >>END_MODULE