FastQCFastQC Report
Thu 26 May 2016
SRR937364_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937364_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1077236
Sequences flagged as poor quality0
Sequence length101
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT92570.8593288750097471No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT69930.6491613722526912No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56190.5216127199610856No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT16820.15614034436279514No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16330.15159166607874225No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA15950.14806411965437472No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14820.13757431055033437No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA98450.030.9981231
GGTATCA65950.029.4077261
GTACATG79600.025.081431
TATCAAC121850.024.8695762
ATCAACG121950.024.8491843
TCAACGC122650.024.7073614
TACCTGG7700.024.6741662
CAACGCA123750.024.487745
TACATGG81500.024.1277032
TATATGG3150.024.1258532
AACGCAG125900.024.0695616
ACATGGG81650.023.3271353
AGTACTT70200.023.21083512-13
GAGTACT68000.022.9837412-13
CATGGGG25150.022.4740964
TAAGGTG3450.022.0279525
GTACTTT71550.021.70757914-15
ACGCAGA139400.021.5712187
AGGTAAG2457.521521E-921.3255312
CGCAGAG140350.021.2898188
Produced by FastQC (version 0.11.5)