##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937364_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1077236 Sequences flagged as poor quality 0 Sequence length 101 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.25743755314527 33.0 31.0 34.0 31.0 34.0 2 32.449124425845405 34.0 31.0 34.0 31.0 34.0 3 32.50017266411446 34.0 31.0 34.0 31.0 34.0 4 36.13766992562447 37.0 35.0 37.0 35.0 37.0 5 36.07166953202455 37.0 35.0 37.0 35.0 37.0 6 36.12254139297239 37.0 35.0 37.0 35.0 37.0 7 36.06560308047633 37.0 35.0 37.0 35.0 37.0 8 36.04551648849463 37.0 35.0 37.0 35.0 37.0 9 37.75473805182894 39.0 37.0 39.0 35.0 39.0 10-11 37.67461958196718 39.0 37.0 39.0 35.0 39.0 12-13 37.581572190309274 39.0 37.0 39.0 35.0 39.0 14-15 38.74855649087108 40.0 38.0 41.0 34.5 41.0 16-17 38.73316710544393 40.0 38.0 41.0 34.5 41.0 18-19 38.77168466334211 40.0 38.0 41.0 35.0 41.0 20-21 38.70420409269649 40.0 38.0 41.0 34.0 41.0 22-23 38.56128647761493 40.0 38.0 41.0 34.0 41.0 24-25 38.490407394479945 40.0 38.0 41.0 34.0 41.0 26-27 38.411730112992885 40.0 38.0 41.0 34.0 41.0 28-29 38.282160083769945 40.0 38.0 41.0 34.0 41.0 30-31 38.30221882670093 40.0 38.0 41.0 34.0 41.0 32-33 38.19068198612003 40.0 37.0 41.0 34.0 41.0 34-35 38.05667838802268 40.0 37.0 41.0 34.0 41.0 36-37 37.869833072789994 40.0 37.0 41.0 33.0 41.0 38-39 37.729853996710105 40.0 37.0 41.0 33.0 41.0 40-41 37.53321463449049 40.0 37.0 41.0 33.0 41.0 42-43 37.275505553100714 40.0 36.0 41.0 33.0 41.0 44-45 36.9128273655912 40.0 36.0 41.0 31.5 41.0 46-47 36.45100052356216 40.0 36.0 41.0 30.5 41.0 48-49 36.04683235614108 39.0 35.0 41.0 29.5 41.0 50-51 35.79297479846571 39.0 35.0 41.0 28.5 41.0 52-53 35.5443709642084 39.0 35.0 41.0 28.0 41.0 54-55 35.311752949214466 39.0 35.0 40.0 27.5 41.0 56-57 35.049950057369045 38.0 35.0 40.0 26.5 41.0 58-59 34.789505270896996 38.0 34.5 40.0 26.0 41.0 60-61 34.46695663717143 38.0 34.0 40.0 26.0 41.0 62-63 34.10071284286823 37.0 34.0 40.0 25.5 41.0 64-65 33.70779522778667 37.0 33.5 39.5 24.0 41.0 66-67 33.29536981682752 36.0 33.0 39.0 22.5 40.5 68-69 32.8725200420335 36.0 33.0 39.0 22.0 40.0 70-71 32.46827204066704 35.0 32.0 38.5 20.5 40.0 72-73 32.00525975737907 35.0 32.0 37.5 19.5 40.0 74-75 31.54406601710303 35.0 31.0 37.0 18.0 39.0 76-77 30.270925776710023 33.5 29.5 35.5 17.0 38.0 78-79 30.86031055404758 34.0 31.0 36.0 16.0 38.5 80-81 30.67328143508015 34.0 31.0 36.0 12.0 37.0 82-83 30.343186636911504 34.0 31.0 35.0 7.5 37.0 84-85 29.99474209922431 34.0 30.5 35.0 4.5 36.5 86-87 29.599026582847213 34.0 30.0 35.0 2.0 36.0 88-89 29.155483106765836 34.0 29.5 35.0 2.0 36.0 90-91 28.67708097389987 34.0 29.0 35.0 2.0 35.0 92-93 28.34954921669903 34.0 29.0 35.0 2.0 35.0 94-95 27.955612790512014 34.0 29.0 35.0 2.0 35.0 96-97 27.520559561693073 33.5 27.5 35.0 2.0 35.0 98-99 26.83643417041391 33.0 26.5 35.0 2.0 35.0 100-101 24.49101079057885 31.0 20.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05811202362168899 1101 2 -0.05783297162924583 1101 3 -0.074253523802156 1101 4 -0.08492234797664366 1101 5 -0.07147546500441848 1101 6 -7.410414035646795E-4 1101 7 -0.07204098894724353 1101 8 0.016267277657647128 1101 9 -0.10461089349941943 1101 10-11 -0.06347968900414713 1101 12-13 -0.12542627127377415 1101 14-15 -0.23154898582874495 1101 16-17 -0.16402372095432582 1101 18-19 -0.16998737293536692 1101 20-21 -0.1276073136764282 1101 22-23 -0.18139753127437785 1101 24-25 -0.10994524639590253 1101 26-27 -0.2026349448565412 1101 28-29 -0.24402570017773684 1101 30-31 -0.16274049747152475 1101 32-33 -0.08106890231164243 1101 34-35 0.03240218832085162 1101 36-37 0.025104125495197138 1101 38-39 -0.012140901122322134 1101 40-41 0.0979293554048084 1101 42-43 -0.10786802477969104 1101 44-45 0.03722317776679773 1101 46-47 -0.2096787865895564 1101 48-49 -0.0721080300839958 1101 50-51 0.26940410012732485 1101 52-53 0.10175177138395242 1101 54-55 0.0026254261616855956 1101 56-57 0.042170676824731856 1101 58-59 -0.060936820469287056 1101 60-61 -0.004035598269481966 1101 62-63 -0.09656502014426138 1101 64-65 -0.2506945785584165 1101 66-67 -0.24777938051699522 1101 68-69 -0.09127589598180208 1101 70-71 -0.11542399482427612 1101 72-73 -0.14949614834418412 1101 74-75 -0.1691123714479552 1101 76-77 -0.1081577902532409 1101 78-79 -0.2087525491189588 1101 80-81 -0.4337303738123559 1101 82-83 -0.295489644296012 1101 84-85 -0.2441700699355458 1101 86-87 -0.5387614815287272 1101 88-89 -0.37333585715938256 1101 90-91 -0.3993760583805894 1101 92-93 -0.5343110499255062 1101 94-95 -0.5286664811654269 1101 96-97 -0.5962421269314824 1101 98-99 -0.3375271535998152 1101 100-101 0.4207470116732104 1102 1 -0.013473041152764154 1102 2 0.05807942932744936 1102 3 0.011150140662159913 1102 4 0.007912142302167524 1102 5 -0.060066350144680314 1102 6 -0.005515220634549678 1102 7 -0.076638561888835 1102 8 -0.05775109055833383 1102 9 -0.10107295829417495 1102 10-11 -0.16918697686019613 1102 12-13 -0.12695356711196126 1102 14-15 -0.1470759900341747 1102 16-17 -0.09068766881364354 1102 18-19 -0.13974122211011064 1102 20-21 -0.1034372325246764 1102 22-23 -0.14510581021703928 1102 24-25 -0.06299208250003119 1102 26-27 -0.10971244190390905 1102 28-29 -0.14819645522190683 1102 30-31 -0.06364374569108833 1102 32-33 -0.12699525167955983 1102 34-35 -0.2747364818918854 1102 36-37 -0.08412715496429968 1102 38-39 -0.0197765089006694 1102 40-41 0.055022160267064635 1102 42-43 -0.19805051600892654 1102 44-45 -0.10096398191027589 1102 46-47 0.04812398717488264 1102 48-49 0.20744229777047707 1102 50-51 0.11656965632385408 1102 52-53 0.06837753975202787 1102 54-55 0.047061079021105456 1102 56-57 0.1231159038945222 1102 58-59 0.17820465220641069 1102 60-61 -0.04893251887159522 1102 62-63 0.008501198500134421 1102 64-65 0.04943154389364679 1102 66-67 0.01907359416615151 1102 68-69 0.12190096650289917 1102 70-71 0.12202054702472509 1102 72-73 -0.06322571880166805 1102 74-75 -0.08134311221257917 1102 76-77 0.009992107820526286 1102 78-79 0.15199774875891947 1102 80-81 -0.029691783014357043 1102 82-83 0.13703135820029289 1102 84-85 0.10765783420674069 1102 86-87 0.27947317594511745 1102 88-89 0.22448253231906534 1102 90-91 0.1127583622433086 1102 92-93 0.08471461782345457 1102 94-95 0.03344868123974365 1102 96-97 0.39006565716500674 1102 98-99 0.3702855833154892 1102 100-101 0.8252178231268239 1103 1 -0.16469954347837046 1103 2 -0.08137232459882426 1103 3 -0.08396492836062208 1103 4 -0.09244339431006665 1103 5 -0.08139155958870248 1103 6 -0.11511203070369902 1103 7 -0.11863508687655155 1103 8 -0.22893212891622738 1103 9 -0.11452523859630759 1103 10-11 -0.22063526741966655 1103 12-13 -0.12886051504035123 1103 14-15 -0.19742785831910226 1103 16-17 -0.16607674441845433 1103 18-19 -0.26637100753043086 1103 20-21 -0.418205918285274 1103 22-23 -0.22017929321813057 1103 24-25 -0.2867222007482795 1103 26-27 -0.46353775033453815 1103 28-29 -0.3313618606827049 1103 30-31 -0.549530293061423 1103 32-33 -0.24318515225041892 1103 34-35 -0.20543450052932144 1103 36-37 -0.0422651353113892 1103 38-39 -0.303204869379627 1103 40-41 -0.16085831980635135 1103 42-43 0.06767816518664915 1103 44-45 -0.10657681383566597 1103 46-47 0.14602261789892879 1103 48-49 0.0960308302299353 1103 50-51 0.2760993884078644 1103 52-53 0.22711916373799568 1103 54-55 0.0343410084960567 1103 56-57 0.034011942333940226 1103 58-59 0.0883451899016805 1103 60-61 0.0520993291967784 1103 62-63 0.034224033662972886 1103 64-65 -0.03058632027808983 1103 66-67 -0.07370975791641143 1103 68-69 -0.0531274484769213 1103 70-71 -0.03354098654570947 1103 72-73 -0.08406007111480918 1103 74-75 0.135657081102444 1103 76-77 0.29325946948965154 1103 78-79 0.09514828293724875 1103 80-81 -0.17780835896271086 1103 82-83 -0.10424355440035171 1103 84-85 -0.23321827881034807 1103 86-87 -0.13070579184580566 1103 88-89 0.1357212391114082 1103 90-91 -0.11546836833377228 1103 92-93 0.07698783949989263 1103 94-95 0.16125262253719086 1103 96-97 0.07383953914035857 1103 98-99 0.40843065576204296 1103 100-101 0.7727307554362888 1104 1 -0.16802788775989796 1104 2 -0.13505775564365763 1104 3 -0.17819795403575966 1104 4 -0.11868673069226787 1104 5 -0.14936739040441438 1104 6 -0.16444589989815483 1104 7 -0.19382194534454555 1104 8 -0.15080958302156944 1104 9 -0.21171473941395647 1104 10-11 -0.273002988254504 1104 12-13 -0.22892680475643346 1104 14-15 -0.30486108355631103 1104 16-17 -0.3376366084205884 1104 18-19 -0.1925434347041275 1104 20-21 -0.2803417867960434 1104 22-23 -0.3120760106068232 1104 24-25 -0.2587581034432844 1104 26-27 -0.3281658350005898 1104 28-29 -0.24715659815695545 1104 30-31 -0.36100996267532537 1104 32-33 -0.23058433182856675 1104 34-35 -0.26236743473850765 1104 36-37 -0.3381771155029938 1104 38-39 -0.31796876712254374 1104 40-41 -0.3161125304736814 1104 42-43 -0.33759798806963204 1104 44-45 -0.25779820762891603 1104 46-47 -0.22084361083985726 1104 48-49 -0.031079122928417746 1104 50-51 -0.047148438120963476 1104 52-53 0.008033961819982949 1104 54-55 0.30114210239186434 1104 56-57 0.19139804874856026 1104 58-59 -0.11969596053670983 1104 60-61 -0.07365249319298073 1104 62-63 -0.09680093931299893 1104 64-65 -0.04757796823581373 1104 66-67 0.023147139447708298 1104 68-69 0.08554278443789087 1104 70-71 0.14040715503249857 1104 72-73 0.22004688861245825 1104 74-75 0.36001658847977325 1104 76-77 0.2676732559028032 1104 78-79 0.12948266027586897 1104 80-81 0.08573137329151237 1104 82-83 0.05787175736918826 1104 84-85 0.21725383331397197 1104 86-87 0.13002528902302757 1104 88-89 0.28627973574379695 1104 90-91 0.16090431383591053 1104 92-93 0.39342653421611473 1104 94-95 0.5188198130542965 1104 96-97 0.4091534798445551 1104 98-99 0.5892687702700208 1104 100-101 1.1827984771950213 1105 1 -0.005687517437586109 1105 2 -0.08020677423391476 1105 3 -0.08496866187406482 1105 4 -0.049355553556615916 1105 5 -0.14810935099974643 1105 6 -0.12118602556308389 1105 7 -0.12562369872248524 1105 8 -0.07985894079592981 1105 9 -0.08333679935501692 1105 10-11 -0.08646447325070028 1105 12-13 -0.08131684794216909 1105 14-15 -0.08177571295597375 1105 16-17 -0.09866009182281488 1105 18-19 -0.035781994191090405 1105 20-21 -0.0015134547292419143 1105 22-23 -0.09898081664170633 1105 24-25 -0.3081992837298273 1105 26-27 -0.10276942516239984 1105 28-29 -0.3447797609097165 1105 30-31 -0.15978737078979321 1105 32-33 -0.1791865874955363 1105 34-35 -0.0673249903887978 1105 36-37 -0.291826588192194 1105 38-39 -0.4014511499372162 1105 40-41 -0.2738651114237669 1105 42-43 -0.07302627750673452 1105 44-45 0.03591113198148577 1105 46-47 0.04819495050056588 1105 48-49 0.23680797324788472 1105 50-51 -0.026530956238580927 1105 52-53 -0.028139883934656496 1105 54-55 -0.043159882669208116 1105 56-57 -0.16276321197836552 1105 58-59 -0.03311829108367448 1105 60-61 -0.10156336005859146 1105 62-63 -0.0569421733500306 1105 64-65 0.1536538909749794 1105 66-67 0.1037751860808811 1105 68-69 0.28801530681847964 1105 70-71 0.3186815327316097 1105 72-73 0.3467034230034969 1105 74-75 0.23514864786479706 1105 76-77 0.5504251418034904 1105 78-79 0.23501432672629718 1105 80-81 0.10556460777337762 1105 82-83 0.07901168471336106 1105 84-85 0.10048090131760645 1105 86-87 0.26380955495819336 1105 88-89 0.3892987986318701 1105 90-91 0.5438649462381875 1105 92-93 0.713401790457663 1105 94-95 0.7514713801589998 1105 96-97 0.452394819529097 1105 98-99 0.542838312278807 1105 100-101 1.0643470009101073 1106 1 -0.03200811651091584 1106 2 0.030606726650269422 1106 3 0.023848010226146243 1106 4 0.046910858227271035 1106 5 -0.016174168284351254 1106 6 0.002691452875609457 1106 7 0.027204709840788155 1106 8 -0.008353452912736259 1106 9 -0.08549910436475017 1106 10-11 -0.0416346173879063 1106 12-13 -0.09709759935242346 1106 14-15 -0.07575188437946423 1106 16-17 0.03826037091786816 1106 18-19 0.1511839127668111 1106 20-21 0.04641167098329646 1106 22-23 -0.020133864861975326 1106 24-25 0.03646546637637016 1106 26-27 -0.045630113938671 1106 28-29 0.015297123790396938 1106 30-31 0.013037110800361518 1106 32-33 -0.01754656991059278 1106 34-35 -0.02845042630751493 1106 36-37 -0.12001410486330855 1106 38-39 -0.0832909985882111 1106 40-41 0.13587748660237509 1106 42-43 -0.05421941049034018 1106 44-45 0.12894705267144246 1106 46-47 -0.18156199703111753 1106 48-49 -0.2506189208374465 1106 50-51 -0.11069167070071018 1106 52-53 0.031343262188258336 1106 54-55 -0.018605186602101753 1106 56-57 -0.1393489434820765 1106 58-59 0.05339490255808954 1106 60-61 0.041667427410665425 1106 62-63 0.16523682746993984 1106 64-65 0.37348967434436986 1106 66-67 0.22823553600135682 1106 68-69 0.13026830267909872 1106 70-71 0.1823766662486932 1106 72-73 7.284469605082222E-4 1106 74-75 -0.07194311981645996 1106 76-77 0.1690055286720913 1106 78-79 -0.049289882791303086 1106 80-81 -0.1324760302239163 1106 82-83 -0.14899417857857955 1106 84-85 -0.14757290068391526 1106 86-87 -0.11891657941838218 1106 88-89 0.0646461752162395 1106 90-91 0.20696769956500205 1106 92-93 0.44420407847520593 1106 94-95 0.45847225940580927 1106 96-97 0.47963294722734773 1106 98-99 0.43050403548400595 1106 100-101 1.050842782501249 1107 1 -0.030836291654246395 1107 2 -0.113275445216523 1107 3 -0.09159389611174618 1107 4 -0.11619076644053195 1107 5 -0.16347522178286056 1107 6 -0.09394787544759708 1107 7 -0.09350468903339504 1107 8 -0.08231631801180583 1107 9 -0.06845998295378308 1107 10-11 -0.0019479470862933113 1107 12-13 -0.08231856205356536 1107 14-15 0.01108167200730037 1107 16-17 -0.06369633511042139 1107 18-19 -0.15158982971387047 1107 20-21 -0.10367437298937432 1107 22-23 -0.09167586944505501 1107 24-25 -0.16818446683469546 1107 26-27 -0.2414348030174409 1107 28-29 -0.1482274127223704 1107 30-31 -0.1453835692393497 1107 32-33 -0.11490589691200626 1107 34-35 -0.04651838960727872 1107 36-37 -0.0749315750942472 1107 38-39 -0.16481480302818596 1107 40-41 -0.28887629672696136 1107 42-43 -0.2542678340554616 1107 44-45 -0.21244194107162429 1107 46-47 -0.2825997991859097 1107 48-49 -0.3795987723130949 1107 50-51 -0.46913122449217326 1107 52-53 -0.46095694612203886 1107 54-55 -0.35891227588830077 1107 56-57 -0.41219698626766643 1107 58-59 -0.43729086917500837 1107 60-61 -0.2878616057575698 1107 62-63 -0.29028466636526673 1107 64-65 -0.1279188667591029 1107 66-67 -0.015589188486352157 1107 68-69 0.016200939272323467 1107 70-71 0.0769019437309737 1107 72-73 0.04347336407486324 1107 74-75 -0.0639843700849525 1107 76-77 0.2593418500317135 1107 78-79 0.22010845758950026 1107 80-81 0.18421853400847965 1107 82-83 0.016032314576666096 1107 84-85 0.19710787871941804 1107 86-87 0.21259896378344578 1107 88-89 0.4490145781409751 1107 90-91 0.2884575058326142 1107 92-93 0.5267185960125431 1107 94-95 0.5394009369595203 1107 96-97 0.4471780377006134 1107 98-99 0.5666727528441733 1107 100-101 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24-25 0.14065986961220034 2316 26-27 0.15335044827367028 2316 28-29 0.37915970256225506 2316 30-31 0.28732166581069407 2316 32-33 0.12786279015128343 2316 34-35 -0.1815626278620215 2316 36-37 -0.16095328094455397 2316 38-39 -0.4232113708605212 2316 40-41 -0.46565737588763767 2316 42-43 -0.3704162989227697 2316 44-45 -0.46020620534521584 2316 46-47 -0.4122890438314286 2316 48-49 -0.6890500770878418 2316 50-51 -1.1825499891355449 2316 52-53 -0.8957016361849455 2316 54-55 -0.7571270018099909 2316 56-57 -0.6014084867638019 2316 58-59 -0.8394943920771851 2316 60-61 -0.8253206664689046 2316 62-63 -0.813505865925265 2316 64-65 -1.354378128412165 2316 66-67 -1.0437096375186243 2316 68-69 -0.9667811603409184 2316 70-71 -0.9358381764611856 2316 72-73 -1.0707019358358494 2316 74-75 -1.0485936834235474 2316 76-77 -0.9935371063970777 2316 78-79 -1.1714575828432885 2316 80-81 -1.3018180871044898 2316 82-83 -1.3821969122948339 2316 84-85 -1.2488097931738018 2316 86-87 -1.3788637055928277 2316 88-89 -1.28154741652682 2316 90-91 -0.9193493912334318 2316 92-93 -0.9136452121026046 2316 94-95 -0.8872014697838395 2316 96-97 -1.1206149384536097 2316 98-99 -0.8402238988865172 2316 100-101 -0.969362119422243 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 3.0 4 6.0 5 21.0 6 43.0 7 125.0 8 264.0 9 502.0 10 888.0 11 1561.0 12 2555.0 13 3858.0 14 4687.0 15 6868.0 16 9777.0 17 14589.0 18 12536.0 19 6529.0 20 5424.0 21 5296.0 22 5505.0 23 6143.0 24 6832.0 25 8216.0 26 9834.0 27 12188.0 28 15127.0 29 18589.0 30 23256.0 31 29285.0 32 37423.0 33 49222.0 34 68833.0 35 100926.0 36 153712.0 37 235724.0 38 200017.0 39 20871.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.8587886000091 20.089237192010458 15.318401013695857 20.73357319428458 2 15.558893315856507 21.624787883063693 39.86972214073796 22.94659666034184 3 19.20275594205912 26.89206450582788 30.135272122357588 23.76990742975541 4 12.55277395111192 17.355528407888336 37.47832415552395 32.61337348547579 5 12.831609894848436 39.270260961913536 33.34651557221592 14.551613571022095 6 30.410142252951072 37.48333698465331 17.30048011763439 14.806040644761223 7 27.931576738987555 34.25321842196139 20.48214133207579 17.333063506975257 8 27.354080257250963 34.2487625738464 19.395842693708715 19.001314475193922 9 27.82695713845434 16.30886825171086 20.44408096276025 35.42009364707455 10-11 24.950115272129953 27.685780288117662 26.598179503329593 20.765924936422792 12-13 26.388105592527143 25.23778556664471 27.78731658680525 20.586792254022896 14-15 23.29484903957907 25.882954153036103 27.168605579464483 23.653591227920344 16-17 21.037915554251807 29.487688862978956 27.724751122316743 21.74964446045249 18-19 21.093103089759346 27.222818398196868 31.462372219272282 20.2217062927715 20-21 22.161717580920058 25.99820280792695 31.61145747078635 20.228622140366642 22-23 21.780369400634868 26.133658364056377 31.430291446174753 20.655680789134 24-25 21.559651976950086 26.493707225261144 31.527694939091326 20.418945858697445 26-27 21.381393591532362 26.54115041249277 31.6104902291152 20.466965766859662 28-29 21.03593398325213 26.630424944405068 31.807356308394407 20.5262847639484 30-31 21.191813833974482 26.59733465028889 31.583261544402692 20.627589971333936 32-33 21.05782762551567 26.778301133642024 31.43837562056968 20.725495620272625 34-35 21.482572064060243 26.544647598112203 31.332874133430373 20.639906204397178 36-37 21.632930604742697 26.347036626177207 31.28760205526185 20.732430713818246 38-39 21.309210947718636 26.720270248944285 31.285548383862672 20.68497041947441 40-41 21.31724458996123 26.82714564669591 31.202427328893872 20.653182434448986 42-43 21.247409161067203 26.924160661026587 30.798500620872936 21.029929557033274 44-45 21.385899369265125 26.989270219928695 30.454946945213003 21.169883465593177 46-47 21.66693277981798 27.366101764144535 29.918977828442422 21.047987627595067 48-49 21.663544478647204 27.507946262471734 29.4363537794875 21.39215547939356 50-51 21.71901050466193 27.741878288508737 29.216299863725308 21.322811343104018 52-53 21.930709705208514 28.027238228206265 28.689210163789554 21.352841902795674 54-55 21.55530450152056 27.947311452643618 28.84196220698157 21.655421838854252 56-57 21.743610499463443 28.213084226668993 28.553353211366865 21.4899520625007 58-59 21.952385549684564 27.582349642975167 28.88424634898945 21.58101845835082 60-61 21.871808962938484 27.825471855749345 29.13920440831907 21.163514772993107 62-63 21.90791989870372 27.82454355405872 29.119385167224266 21.148151380013296 64-65 21.691718434957615 27.809551481755157 29.0796538548656 21.419076228421627 66-67 21.77624030388884 27.968848051866075 28.628916968983585 21.625994675261502 68-69 21.809009353567834 27.980776728590577 28.78333995521873 21.42687396262286 70-71 21.824976142646552 28.12470990572168 28.63448677912732 21.415827172504446 72-73 21.848183684912126 27.977527672673396 28.465860777025647 21.70842786538883 74-75 21.617082979031522 28.543466798361735 28.184214044090616 21.65523617851613 76-77 21.586263362902834 28.10256991040032 28.690556201240952 21.620610525455888 78-79 21.5501524271376 28.0228287951758 28.59340942931725 21.833609348369347 80-81 21.539719030796885 28.570307978153338 28.289264510870648 21.600708480179126 82-83 21.60288368306407 28.30695480599146 28.25162789683017 21.838533614114304 84-85 21.48407420695942 28.14536907374977 28.06107884988403 22.30947786940678 86-87 21.46265999279638 28.10308047633016 28.204961586876042 22.229297943997416 88-89 21.535624505679348 27.800546955356115 28.313480054509878 22.35034848445466 90-91 21.364623180797185 27.78436148203438 28.453439130598255 22.39757620657018 92-93 21.39130265263107 27.696737926139992 28.567317435215955 22.344641986012988 94-95 21.066971397168306 27.540761727235257 28.639221117749496 22.753045757846934 96-97 21.213967969878468 27.45661117898028 28.58533320460883 22.74408764653242 98-99 21.176633314287653 27.52641138025193 28.32080665816032 22.9761486473001 100-101 21.0018071111891 27.551598757257107 28.553053080235646 22.89354105131815 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1114.0 1 774.5 2 596.5 3 792.5 4 636.5 5 577.0 6 1075.5 7 2245.0 8 3383.5 9 3625.5 10 3085.0 11 2559.0 12 2415.0 13 2489.0 14 2613.5 15 2628.5 16 2665.5 17 2732.5 18 2839.0 19 2903.5 20 3017.0 21 3322.0 22 3707.0 23 4260.5 24 4919.5 25 5706.5 26 6931.0 27 8181.0 28 9108.0 29 10723.0 30 12975.0 31 14898.5 32 17341.0 33 20092.0 34 23069.0 35 25401.0 36 28910.5 37 32550.0 38 33724.5 39 34086.5 40 33553.0 41 33723.5 42 34111.0 43 35224.0 44 37287.5 45 38822.0 46 38317.0 47 37973.5 48 42258.5 49 42236.0 50 38290.5 51 35196.0 52 31808.0 53 30788.0 54 30066.5 55 28490.5 56 26260.5 57 23663.0 58 21755.5 59 19921.5 60 17136.0 61 14226.5 62 11300.0 63 8284.0 64 5873.0 65 4252.0 66 3107.5 67 2534.0 68 1993.5 69 1334.0 70 909.5 71 638.0 72 473.5 73 392.0 74 301.5 75 227.5 76 151.0 77 81.5 78 55.0 79 36.0 80 21.5 81 13.5 82 10.5 83 7.5 84 4.5 85 3.0 86 2.5 87 2.0 88 0.5 89 0.0 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07231470169953473 2 0.0 3 0.0 4 0.0 5 0.002692074902806813 6 0.0 7 0.0 8 0.0 9 0.0 10-11 2.3207542265575977E-4 12-13 0.005337734721082474 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 4.177357607803675E-4 24-25 7.890564370295832E-4 26-27 4.6415084531151955E-4 28-29 0.0022743391420264455 30-31 3.713206762492156E-4 32-33 0.0 34-35 0.0 36-37 9.28301690623039E-5 38-39 6.498111834361273E-4 40-41 2.3207542265575977E-4 42-43 0.011464525879194531 44-45 4.641508453115195E-5 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 4.641508453115195E-5 82-83 2.3207542265575977E-4 84-85 5.105659298426714E-4 86-87 0.0 88-89 0.0 90-91 0.007565658778577768 92-93 0.002088678803901838 94-95 0.0 96-97 0.0 98-99 1.3924525359345583E-4 100-101 0.00951509232888615 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1077236.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 49.04247391648354 #Duplication Level Percentage of deduplicated Percentage of total 1 73.13486271113243 35.86714596896317 2 14.189130367220038 13.917401118639464 3 5.119302281152663 7.531897457820724 4 2.362700164542127 4.6349064476811455 5 1.3337900262549598 3.2706181286337377 6 0.837979058574372 2.4657939673615847 7 0.5498257583565699 1.8875370788969 8 0.3934318607770397 1.5435897416057238 9 0.285228092061768 1.258946213866886 >10 1.5995264935396518 14.39084429817688 >50 0.12987103248438275 4.450338190561035 >100 0.060945275538558115 5.414469905140402 >500 0.0020819812233574342 0.7432352884525558 >1k 7.570840812208852E-4 0.5933258353676454 >5k 5.678130609156639E-4 2.02995035883214 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9257 0.8593288750097471 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6993 0.6491613722526912 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5619 0.5216127199610856 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 1682 0.15614034436279514 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1633 0.15159166607874225 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 1595 0.14806411965437472 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1482 0.13757431055033437 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 4.641508453115195E-5 0.0 12-13 0.0 0.0 0.0 1.3924525359345586E-4 0.0 14-15 0.0 0.0 0.0 1.856603381246078E-4 0.0 16-17 0.0 0.0 0.0 2.784905071869117E-4 0.0 18-19 0.0 0.0 0.0 4.641508453115195E-4 0.0 20-21 0.0 0.0 0.0 5.105659298426714E-4 0.0 22-23 0.0 0.0 0.0 6.962262679672793E-4 0.0 24-25 0.0 0.0 0.0 7.890564370295831E-4 0.0 26-27 0.0 0.0 0.0 0.0014388676204657104 0.0 28-29 0.0 0.0 0.0 0.0020422637193706857 0.0 30-31 0.0 0.0 0.0 0.0037596218470233077 0.0 32-33 0.0 0.0 0.0 0.005152074382957866 0.0 34-35 0.0 0.0 0.0 0.006683772172485881 0.0 36-37 0.0 0.0 0.0 0.008726035891856566 0.0 38-39 0.0 0.0 0.0 0.010907544864820706 0.0 40-41 0.0 0.0 0.0 0.01503848738809323 0.0 42-43 0.0 0.0 0.0 0.021118863461674138 0.0 44-45 0.0 0.0 0.0 0.03430074746852129 0.0 46-47 0.0 0.0 0.0 0.06043244005955983 0.0 48-49 0.0 0.0 0.0 0.0831758314798243 0.0 50-51 0.0 0.0 0.0 0.11561997556709949 0.0 52-53 0.0 0.0 0.0 0.1561867594473263 0.0 54-55 0.0 0.0 0.0 0.19949203331489107 0.0 56-57 0.0 0.0 0.0 0.24878485308697446 0.0 58-59 0.0 0.0 0.0 0.32123880004010263 0.0 60-61 0.0 0.0 0.0 0.41165538470678664 0.0 62-63 0.0 0.0 0.0 0.5170640416770327 0.0 64-65 0.0 0.0 0.0 0.6060881738077821 0.0 66-67 0.0 0.0 0.0 0.7413881452160901 0.0 68-69 0.0 0.0 0.0 0.9039801863287154 0.0 70-71 0.0 0.0 0.0 1.0409046856956135 0.0 72-73 0.0 0.0 0.0 1.2579880360478113 0.0 74-75 0.0 0.0 0.0 1.4450408267083537 0.0 76-77 0.0 0.0 0.0 1.6140845645708088 0.0 78-79 0.0 0.0 0.0 1.8295898020489476 0.0 80-81 0.0 0.0 0.0 2.071273147202656 0.0 82-83 0.0 0.0 0.0 2.3401093168070877 0.0 84-85 0.0 0.0 0.0 2.6954167888930556 0.0 86-87 0.0 0.0 0.0 3.1454574485071056 0.0 88-89 0.0 0.0 0.0 3.666930923214597 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 9845 0.0 30.998123 1 GGTATCA 6595 0.0 29.407726 1 GTACATG 7960 0.0 25.08143 1 TATCAAC 12185 0.0 24.869576 2 ATCAACG 12195 0.0 24.849184 3 TCAACGC 12265 0.0 24.707361 4 TACCTGG 770 0.0 24.674166 2 CAACGCA 12375 0.0 24.48774 5 TACATGG 8150 0.0 24.127703 2 TATATGG 315 0.0 24.125853 2 AACGCAG 12590 0.0 24.069561 6 ACATGGG 8165 0.0 23.327135 3 AGTACTT 7020 0.0 23.210835 12-13 GAGTACT 6800 0.0 22.98374 12-13 CATGGGG 2515 0.0 22.474096 4 TAAGGTG 345 0.0 22.027952 5 GTACTTT 7155 0.0 21.707579 14-15 ACGCAGA 13940 0.0 21.571218 7 AGGTAAG 245 7.521521E-9 21.325531 2 CGCAGAG 14035 0.0 21.289818 8 >>END_MODULE