FastQCFastQC Report
Thu 26 May 2016
SRR937363_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937363_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460720
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53160.3639301166548004No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT40390.276507475765376No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27610.18901637548606168No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25530.17477682238895886No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA60850.038.6670151
GGTATCA35600.038.1866951
ATCAACG76450.030.5745453
TCAACGC76650.030.4968594
TATCAAC78000.029.785272
CAACGCA78250.029.7528695
AACGCAG80300.029.046496
GTACATG58400.025.8827421
CGCAGAG91250.025.2008198
ACGCAGA91700.025.077157
TACATGG59500.024.9129372
ACATGGG59200.023.914813
GCAGAGT96450.023.2017579
GAGTACT50500.022.15576712-13
AGAGTAC82700.021.68733410-11
AGTACTT53900.021.50742112-13
GTACTTT53750.020.8556614-15
CATGGGA32200.019.9195774
CATGGGG28150.019.9161684
ATGGGCG1951.0304248E-519.492725
Produced by FastQC (version 0.11.5)