##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937363_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1460720 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.490046689303906 31.0 31.0 34.0 30.0 34.0 2 31.61725108165836 33.0 31.0 34.0 30.0 34.0 3 31.641376170655565 33.0 31.0 34.0 30.0 34.0 4 35.28057670190043 37.0 35.0 37.0 33.0 37.0 5 35.28079097979079 37.0 35.0 37.0 33.0 37.0 6 35.309771893312885 37.0 35.0 37.0 33.0 37.0 7 35.23938605619147 37.0 35.0 37.0 32.0 37.0 8 35.21098978585903 37.0 35.0 37.0 32.0 37.0 9 36.77492743304672 39.0 37.0 39.0 33.0 39.0 10-11 36.64398995016157 39.0 37.0 39.0 32.0 39.0 12-13 36.48626465030944 39.0 37.0 39.0 32.0 39.0 14-15 37.538047332822174 40.0 37.0 41.0 32.0 41.0 16-17 37.44221479818172 40.0 37.0 41.0 32.0 41.0 18-19 37.34629463552221 40.0 37.0 41.0 32.0 41.0 20-21 37.19911105482228 39.5 37.0 41.0 31.5 41.0 22-23 37.28013445424175 40.0 37.0 41.0 32.0 41.0 24-25 37.244565693630534 40.0 37.0 41.0 31.0 41.0 26-27 37.148524700147874 39.5 37.0 41.0 31.0 41.0 28-29 36.99985760446903 39.0 36.0 41.0 31.0 41.0 30-31 36.726960676926446 39.0 36.0 41.0 30.0 41.0 32-33 36.46954789418917 39.0 35.5 40.0 30.0 41.0 34-35 36.45464394271318 39.0 35.5 41.0 30.0 41.0 36-37 36.294947697026124 39.0 35.0 41.0 30.0 41.0 38-39 36.13134858152144 39.0 35.0 40.0 29.5 41.0 40-41 35.8815608056301 39.0 35.0 40.0 28.5 41.0 42-43 35.59165753874802 38.0 35.0 40.0 27.0 41.0 44-45 35.331650487430856 38.0 35.0 40.0 26.5 41.0 46-47 34.932157086916035 38.0 34.0 40.0 24.5 41.0 48-49 34.573150911879075 38.0 34.0 40.0 24.0 41.0 50-51 33.61522707979627 37.5 32.5 39.5 22.5 40.5 52-53 33.71902246837176 37.5 33.0 39.5 23.0 40.0 54-55 33.998119420559725 38.0 33.0 40.0 22.5 41.0 56-57 33.69060189495591 38.0 33.0 40.0 20.5 41.0 58-59 33.37741285119667 37.5 33.0 40.0 19.0 41.0 60-61 32.99838949285284 37.0 32.0 40.0 17.5 41.0 62-63 32.55985541376855 36.5 31.0 39.5 13.5 41.0 64-65 32.09822827098965 36.0 31.0 39.0 9.5 40.5 66-67 31.61798599320883 35.5 30.5 39.0 7.5 40.0 68-69 31.068653129963305 35.0 30.0 38.5 4.5 40.0 70-71 30.551022098691057 35.0 29.0 38.0 2.0 40.0 72-73 30.008040897639518 34.5 29.0 37.0 2.0 39.0 74-75 29.39523180349417 34.0 28.0 36.5 2.0 39.0 76-77 28.80485205925845 34.0 27.5 36.0 2.0 39.0 78-79 28.192938413932858 34.0 26.0 35.5 2.0 38.0 80-81 27.538346500355992 33.0 26.0 35.0 2.0 37.0 82-83 26.897993797579275 32.5 25.0 35.0 2.0 36.5 84-85 26.219418163645326 32.0 23.5 35.0 2.0 36.0 86-87 25.523300153349034 32.0 20.5 35.0 2.0 36.0 88-89 24.85352737006408 31.0 18.5 34.0 2.0 35.0 90-91 24.18521790623802 31.0 11.0 34.0 2.0 35.0 92-93 23.405312106358508 30.5 4.5 34.0 2.0 35.0 94-95 22.515944191905362 30.0 2.0 34.0 2.0 35.0 96-97 21.532965250013692 29.0 2.0 34.0 2.0 35.0 98-99 20.240077838326307 29.0 2.0 34.0 2.0 35.0 100-101 17.255749561859904 22.5 2.0 31.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.23687233448984202 1101 2 0.326001831457134 1101 3 0.33428765266738125 1101 4 0.2561213649427714 1101 5 0.10730642059322548 1101 6 0.18995721721908865 1101 7 0.1974917307957753 1101 8 0.0970786452905088 1101 9 0.16427141304659898 1101 10-11 0.33111824940719714 1101 12-13 0.31633591833425356 1101 14-15 0.5194898105120558 1101 16-17 0.6025126673888579 1101 18-19 0.574384581237986 1101 20-21 0.8211403357316769 1101 22-23 0.6495676387894136 1101 24-25 0.603084856810078 1101 26-27 0.7285215018553188 1101 28-29 0.7577604663083193 1101 30-31 0.7757229247894557 1101 32-33 0.6492719237702502 1101 34-35 0.761966292367255 1101 36-37 0.6029802992749822 1101 38-39 0.663051689663881 1101 40-41 0.740913329164151 1101 42-43 0.8748718271440126 1101 44-45 1.047358759909912 1101 46-47 1.0897017057269025 1101 48-49 1.17004347688529 1101 50-51 1.2725383037201894 1101 52-53 1.2004533894792857 1101 54-55 1.2358875364838084 1101 56-57 1.3030816565709529 1101 58-59 1.2658365995628884 1101 60-61 1.2808158622415675 1101 62-63 1.4675088127402773 1101 64-65 1.4753470053059132 1101 66-67 1.443970199260491 1101 68-69 1.518377939518377 1101 70-71 1.550348688773095 1101 72-73 1.683371443606493 1101 74-75 1.8722601512425605 1101 76-77 1.683345265139522 1101 78-79 1.7390573833703904 1101 80-81 1.9031991130311283 1101 82-83 1.8402174978564716 1101 84-85 1.9907342137840516 1101 86-87 1.9571407971743149 1101 88-89 2.152247444787509 1101 90-91 2.3016459976176478 1101 92-93 2.4303966714143534 1101 94-95 2.553497772893735 1101 96-97 2.2707519625475037 1101 98-99 2.510212999681702 1101 100-101 2.530868456825168 1102 1 0.3602703680700188 1102 2 0.40965914325314756 1102 3 0.1898978906492772 1102 4 0.20018649230907926 1102 5 0.2953908613948215 1102 6 0.2620984100277326 1102 7 0.06591662637222839 1102 8 0.07607152623513969 1102 9 0.24973694343232467 1102 10-11 0.2966108744380662 1102 12-13 0.2955836392701485 1102 14-15 0.4389959864447093 1102 16-17 0.6085167978010801 1102 18-19 0.603521883441708 1102 20-21 0.5196207806994764 1102 22-23 0.48499182926082796 1102 24-25 0.495881868882762 1102 26-27 0.6564069741862042 1102 28-29 0.6914063246215747 1102 30-31 0.8328559390955661 1102 32-33 0.6718886150738328 1102 34-35 0.7033192708096507 1102 36-37 0.6961336220377063 1102 38-39 0.5596195819725054 1102 40-41 0.7065981579524845 1102 42-43 0.9429753093606834 1102 44-45 0.8305207085768345 1102 46-47 0.8255101247525758 1102 48-49 0.8080708292337917 1102 50-51 0.7617253211974813 1102 52-53 0.7699244891210029 1102 54-55 1.1065152589229612 1102 56-57 1.0124987458830574 1102 58-59 1.2114615481117 1102 60-61 1.0928565149469165 1102 62-63 1.0449968134274883 1102 64-65 1.0765360199842675 1102 66-67 1.1445706123017132 1102 68-69 1.2363667079468534 1102 70-71 1.3542602320941093 1102 72-73 1.5021571238896776 1102 74-75 1.4483195089169314 1102 76-77 1.633160040848118 1102 78-79 1.717239035606859 1102 80-81 1.9687801446319995 1102 82-83 1.8752014450845529 1102 84-85 1.8879469013795713 1102 86-87 1.9395505741535288 1102 88-89 2.097562612419967 1102 90-91 2.286419308139461 1102 92-93 2.3777693498470853 1102 94-95 2.295626903753355 1102 96-97 2.2000982908114786 1102 98-99 2.310145531509857 1102 100-101 2.28066533646696 1103 1 0.24398765902510178 1103 2 0.24324066755537999 1103 3 0.18699105515558756 1103 4 0.1322617213259889 1103 5 0.15020557564174908 1103 6 0.17774015755892236 1103 7 0.17823780739919926 1103 8 0.19497953360941267 1103 9 0.243192990764868 1103 10-11 0.11609874833228417 1103 12-13 0.07179910809383472 1103 14-15 0.21604391921878374 1103 16-17 0.13531284770603946 1103 18-19 0.07757115959108063 1103 20-21 0.05505301884847569 1103 22-23 -0.10947675147745173 1103 24-25 0.03508306077107193 1103 26-27 -0.005708055896825215 1103 28-29 -0.0937840345169505 1103 30-31 -0.07953491166114901 1103 32-33 0.03693906310379447 1103 34-35 0.22971123628204992 1103 36-37 0.22197430344339608 1103 38-39 0.253882011766855 1103 40-41 0.3514779687366385 1103 42-43 0.19185702087798262 1103 44-45 0.26640383492556907 1103 46-47 0.19184964142328909 1103 48-49 0.11962242770748333 1103 50-51 0.29077081017180717 1103 52-53 0.3323049787384278 1103 54-55 0.7010138512371427 1103 56-57 0.6135073492014911 1103 58-59 0.3799811914026847 1103 60-61 0.4481142572189398 1103 62-63 0.5794390268526683 1103 64-65 0.4341861135918066 1103 66-67 0.7208182363852913 1103 68-69 0.8172793456785499 1103 70-71 0.6830859934592048 1103 72-73 0.8627437072861319 1103 74-75 0.7752916981899389 1103 76-77 0.6584701604656829 1103 78-79 0.680547025831661 1103 80-81 0.7009196841686816 1103 82-83 1.0586404144012853 1103 84-85 0.9427955572897098 1103 86-87 1.1193577855064802 1103 88-89 1.1425956966095825 1103 90-91 0.8218897753196579 1103 92-93 1.1418451362218285 1103 94-95 1.1651590804500849 1103 96-97 1.2853651080123996 1103 98-99 1.2313422788266841 1103 100-101 1.1721806352250503 1104 1 0.36207603692267654 1104 2 0.29672130684981113 1104 3 0.07631565595151102 1104 4 0.25062659960192946 1104 5 0.21442358713905207 1104 6 0.22866307961776045 1104 7 0.17822609428945668 1104 8 0.1610594326353052 1104 9 0.21586382114626446 1104 10-11 0.22989249679715584 1104 12-13 0.36407544803809344 1104 14-15 0.5327552258481134 1104 16-17 0.4011017646906154 1104 18-19 0.313473544664177 1104 20-21 0.30156890111539525 1104 22-23 0.3604010346454132 1104 24-25 0.3564522807063497 1104 26-27 0.3080277659162505 1104 28-29 0.30604349881571835 1104 30-31 0.3716386354518164 1104 32-33 0.4611579051670631 1104 34-35 0.5177712116632236 1104 36-37 0.6360898784718927 1104 38-39 0.5871748224730666 1104 40-41 0.5787976613846482 1104 42-43 0.5647662307896937 1104 44-45 0.5986092786110646 1104 46-47 0.6489123981356144 1104 48-49 0.7608534258490067 1104 50-51 0.6232147753647013 1104 52-53 0.5141290009777251 1104 54-55 0.7387456820742884 1104 56-57 0.8466445530195372 1104 58-59 0.8354282488565516 1104 60-61 0.897043678988851 1104 62-63 0.7540962231743222 1104 64-65 0.7781657171633753 1104 66-67 1.0373627199365316 1104 68-69 0.7925540358490508 1104 70-71 0.8114515603240235 1104 72-73 1.01651110480352 1104 74-75 0.9068322276792884 1104 76-77 0.9461798073828618 1104 78-79 0.7891254234498533 1104 80-81 0.975643574227302 1104 82-83 0.9284813219756209 1104 84-85 0.9946482518018662 1104 86-87 1.2444708347625948 1104 88-89 1.2464551307740983 1104 90-91 1.2901307430922806 1104 92-93 1.604023682348057 1104 94-95 1.7851366409713663 1104 96-97 1.4186665083324144 1104 98-99 1.6761660781619447 1104 100-101 1.7079879666379085 1105 1 0.1790281188406695 1105 2 0.014555858697999469 1105 3 0.20573249363265944 1105 4 0.08866397521309466 1105 5 0.21189867417163555 1105 6 0.1719910507817275 1105 7 0.21749512617735434 1105 8 0.09695874587868758 1105 9 0.16991906439749727 1105 10-11 0.28095343192664046 1105 12-13 0.41853655001669665 1105 14-15 0.4366929003092892 1105 16-17 0.3867420041930316 1105 18-19 0.41546781406567135 1105 20-21 0.49227251864370913 1105 22-23 0.6172810968794309 1105 24-25 0.2862195367423155 1105 26-27 0.32937582937523757 1105 28-29 0.23770012754826553 1105 30-31 0.45944949754080966 1105 32-33 0.4042841789756224 1105 34-35 0.5707412316217599 1105 36-37 0.46271938542218294 1105 38-39 0.4211261546260161 1105 40-41 0.5444059742725074 1105 42-43 0.669554694968177 1105 44-45 0.543223114416044 1105 46-47 0.5480644244372286 1105 48-49 0.5775195058450606 1105 50-51 0.5279585352427674 1105 52-53 0.572488001150198 1105 54-55 0.6524317100443895 1105 56-57 0.7646494740126002 1105 58-59 0.7604535050262768 1105 60-61 0.8364698799329204 1105 62-63 0.7258156491288617 1105 64-65 0.8070519112786059 1105 66-67 0.7143753814616218 1105 68-69 0.7555177549652434 1105 70-71 0.6963943071392826 1105 72-73 0.8563841727505341 1105 74-75 0.9648981858825287 1105 76-77 0.9089386611223595 1105 78-79 0.9934800447129106 1105 80-81 1.0361684449544342 1105 82-83 1.303887268703587 1105 84-85 1.3738963043487793 1105 86-87 1.315205182097408 1105 88-89 1.5329185278081248 1105 90-91 1.4983492943209455 1105 92-93 1.6173966042140897 1105 94-95 1.7909640401314881 1105 96-97 1.9110592092113627 1105 98-99 1.9624645620877565 1105 100-101 1.9428539769374957 1106 1 -0.027503542592409502 1106 2 -0.4381809166991495 1106 3 -0.13351016688186945 1106 4 0.11582380973199946 1106 5 -0.05632952454809015 1106 6 -0.10762928260145088 1106 7 0.04686303680046677 1106 8 0.07624014207205221 1106 9 0.16306840325636074 1106 10-11 -0.3734345941914654 1106 12-13 -0.09516014392158922 1106 14-15 -0.07781695806338718 1106 16-17 0.03397995091723516 1106 18-19 0.14643677567889313 1106 20-21 0.10625581388220695 1106 22-23 0.14580558081907924 1106 24-25 0.17953513995794879 1106 26-27 0.019687993375264057 1106 28-29 0.326668291662223 1106 30-31 0.4048912907748772 1106 32-33 0.28795395731354745 1106 34-35 0.4121175587103849 1106 36-37 0.5463670901195954 1106 38-39 0.6190188340099496 1106 40-41 0.6098724757428968 1106 42-43 0.3902270153350216 1106 44-45 0.5240304400164817 1106 46-47 0.5787311195860099 1106 48-49 0.5977535576397912 1106 50-51 0.5405618719227547 1106 52-53 0.4674377658167117 1106 54-55 0.5720149454704497 1106 56-57 0.3527190273418128 1106 58-59 0.24731078825956132 1106 60-61 0.4352857359273372 1106 62-63 0.4591763964996147 1106 64-65 0.4723069330318168 1106 66-67 0.7496319697312543 1106 68-69 0.7101055119273418 1106 70-71 0.743861534903651 1106 72-73 0.6745653759775614 1106 74-75 0.47448847063249033 1106 76-77 0.5608216850686958 1106 78-79 0.7630639921918849 1106 80-81 0.7550289466669184 1106 82-83 0.8255073315066532 1106 84-85 0.8076810792332516 1106 86-87 0.8263480982389311 1106 88-89 1.1737957057999928 1106 90-91 1.0874032608117332 1106 92-93 1.112854774665319 1106 94-95 1.1671985618811433 1106 96-97 1.053762802942451 1106 98-99 0.8119098728632537 1106 100-101 0.7122300049638994 1107 1 0.16471662742742055 1107 2 0.20961384968305197 1107 3 0.2260457260089339 1107 4 0.1452354057983598 1107 5 0.004744161940841707 1107 6 0.10243393908872633 1107 7 0.22635465442571245 1107 8 6.901864727311136E-4 1107 9 0.08061752889630469 1107 10-11 0.08707693457508725 1107 12-13 0.21110221498351933 1107 14-15 0.1566841954941509 1107 16-17 0.2784782447701559 1107 18-19 0.2463210974021095 1107 20-21 0.2068314100661155 1107 22-23 0.1660555505377488 1107 24-25 0.3062164706738315 1107 26-27 0.05035742944007637 1107 28-29 0.015725227019281363 1107 30-31 -0.08063459026857345 1107 32-33 0.003328058997190908 1107 34-35 0.30090676407802874 1107 36-37 0.19879203362430076 1107 38-39 0.20353009329054572 1107 40-41 0.16682915515997365 1107 42-43 0.1536296409595579 1107 44-45 0.08561908835093135 1107 46-47 0.319540824520395 1107 48-49 0.20875023278523486 1107 50-51 0.24342297413461012 1107 52-53 0.2882833639859328 1107 54-55 0.33154173961256816 1107 56-57 0.3531393824182132 1107 58-59 0.3174224672501964 1107 60-61 0.42482075799150465 1107 62-63 0.5078245231064074 1107 64-65 0.34790727390287657 1107 66-67 0.808767471709757 1107 68-69 0.8301215449078327 1107 70-71 1.0083209611422639 1107 72-73 1.0563647895589376 1107 74-75 0.8746197375246361 1107 76-77 0.9270133110795413 1107 78-79 1.0603041263901538 1107 80-81 1.2301552502872752 1107 82-83 1.4314346606070494 1107 84-85 1.1800192783647319 1107 86-87 1.2857598233145282 1107 88-89 1.5040326384191864 1107 90-91 1.4299111214158273 1107 92-93 1.393038275961505 1107 94-95 1.2097149872647144 1107 96-97 0.9518021493243474 1107 98-99 1.320948761262386 1107 100-101 1.4306173680797336 1108 1 0.07083474999500439 1108 2 0.15799216841422847 1108 3 0.17364630830327954 1108 4 0.1398354604061396 1108 5 0.05859082725881848 1108 6 0.05788403592558211 1108 7 0.14365852973889304 1108 8 -0.012402594407191714 1108 9 0.1660525307849383 1108 10-11 0.10811755229375564 1108 12-13 0.156244739871795 1108 14-15 0.38482463793034327 1108 16-17 0.326028923193995 1108 18-19 0.2894969641591345 1108 20-21 0.21552161560922656 1108 22-23 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-0.09576865135110779 2316 64-65 0.20813312620101954 2316 66-67 0.1807345505196949 2316 68-69 0.4917546445200607 2316 70-71 0.3850831356432316 2316 72-73 0.2894929672244686 2316 74-75 0.4905267253852834 2316 76-77 0.5225049561676904 2316 78-79 0.7735528029220156 2316 80-81 1.0319756420491828 2316 82-83 0.7951835838113901 2316 84-85 1.1160147999172594 2316 86-87 1.1757125848959937 2316 88-89 1.6952232996292373 2316 90-91 1.6521211662258644 2316 92-93 1.8469804983655003 2316 94-95 1.6826235113964394 2316 96-97 2.0674976123400945 2316 98-99 2.5919405252134418 2316 100-101 2.922193439060056 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7045.0 3 1381.0 4 1504.0 5 1712.0 6 1938.0 7 2259.0 8 2781.0 9 3557.0 10 4497.0 11 5637.0 12 7091.0 13 8637.0 14 9893.0 15 11691.0 16 14197.0 17 17649.0 18 15761.0 19 13109.0 20 13761.0 21 14943.0 22 16329.0 23 18310.0 24 20543.0 25 23443.0 26 26770.0 27 30937.0 28 36280.0 29 42021.0 30 49790.0 31 59536.0 32 72653.0 33 90026.0 34 114907.0 35 147913.0 36 190297.0 37 216753.0 38 133322.0 39 11847.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.307623622694074 19.72131748816214 15.26194397776804 22.709114911375746 2 14.71231556313487 20.100837843849632 41.23531850973091 23.95152808328459 3 18.698284484847736 24.875896493797057 30.042282035386748 26.38353698596846 4 12.195413298769914 16.6912894416351 38.20008430325556 32.91321295633943 5 13.162935217502342 37.7817809351461 34.794539168545434 14.26074467880612 6 31.175708031922085 36.832414545150094 17.845993577975985 14.145883844951834 7 27.939296324331224 32.914669940576886 21.16057280618621 17.985460928905685 8 26.05941610558166 35.37186021726016 19.905198308749906 18.663525368408266 9 25.936536523894894 16.89698578688286 20.703805996579188 36.462671692643056 10-11 24.295444865774428 27.087925721457523 27.818555152758144 20.798074260009905 12-13 25.28247031680944 24.301112242422246 28.40097693120473 22.015440509563582 14-15 22.340216005369253 25.704577178205117 26.61894226268987 25.33626455373576 16-17 21.54986095170452 29.061042287494576 27.326344033447075 22.062752727353825 18-19 21.50048664747065 27.56743849836063 29.772395818502623 21.159679035666095 20-21 22.208349930335476 26.57204917310047 29.953446052280814 21.26615484428324 22-23 21.871540282222053 26.511108663828047 29.863863589814315 21.75348746413558 24-25 21.58289375498546 26.72910823491067 29.9453636277865 21.742634382317373 26-27 21.449170550832193 26.903835769270618 29.86109633988444 21.78589734001275 28-29 21.39267427472602 27.03242449759426 29.92555960687347 21.64934162080625 30-31 21.592274059482055 26.845995977788604 29.80661125258252 21.755118710146817 32-33 21.423492649153115 26.853840277877865 29.7490533910118 21.973613681957225 34-35 21.836594771587226 26.861998370710676 29.67200772505502 21.629399132647073 36-37 21.720995230532278 26.732761930445577 29.641173122311574 21.905069716710567 38-39 21.514724775599188 27.04350108148069 29.670512884285856 21.771261258634265 40-41 21.853572658626646 26.930338885603295 29.545460782605236 21.670627673164823 42-43 21.49036958862967 26.92881843006059 29.448586513821617 22.13222546748812 44-45 21.678213714970312 27.04963252322161 29.292712699786122 21.979441062021955 46-47 21.83799074179871 27.290625925365486 28.85719785519366 22.01418547764214 48-49 21.874507793463188 27.310595493129615 28.705159233854683 22.109737479552518 50-51 21.891265417290427 27.482570714132255 28.62435453127113 22.001809337306188 52-53 22.053830099820132 27.446794634153036 28.46051132072881 22.03886394529802 54-55 21.842950945118446 27.53027004119505 28.503784076851673 22.12299493683483 56-57 21.900299963225002 27.685775485896514 28.366147242698514 22.047777308179967 58-59 21.97968235543907 27.322007946087616 28.554910360407177 22.143399338066136 60-61 21.877937106642097 27.49852158486103 28.67144333107855 21.952097977418322 62-63 21.76941036349129 27.525678205822203 28.797153322781444 21.907758107905064 64-65 21.678787500437338 27.449761024859313 28.716394442413062 22.155057032290284 66-67 21.714161048455317 27.49691104142326 28.457626897711393 22.33130101241003 68-69 21.66705714753213 27.40894392134492 28.681065405602133 22.24293352552081 70-71 21.769031149287088 27.34873445837357 28.627568688457387 22.254665703881955 72-73 21.72913899469796 27.363552037937584 28.53229250139961 22.37501646596485 74-75 21.719961042520804 27.640530461174084 28.322492180888126 22.31701631541699 76-77 21.82798764698879 27.382897028847598 28.439305247765294 22.349810076398317 78-79 21.775983169197325 27.447252780431036 28.31812742224125 22.45863662813039 80-81 21.752338620838948 27.638729694986964 28.231417025660587 22.3775146585135 82-83 21.7996594280257 27.563003070910874 28.28284214310609 22.354495357957337 84-85 21.830648718471124 27.479144243427033 28.22790037530415 22.462306662797694 86-87 21.77104038718463 27.490108578396484 28.316923377712534 22.42192765670636 88-89 21.903718703913285 27.341140938607307 28.154274017236926 22.60086634024249 90-91 21.824170162024277 27.268351870992575 28.252380656707572 22.65509731027558 92-93 21.843346586757324 27.052937079969947 28.438268661427045 22.66544767184568 94-95 21.672032981395482 27.017788081224026 28.416025807708696 22.8941531296718 96-97 21.770540687874995 26.96821563704067 28.319600831737773 22.94164284334656 98-99 21.62284653266628 27.004156148210562 28.25151524092554 23.121482078197616 100-101 22.126134370995228 26.119072251234186 28.81264595550604 22.942147422264544 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2317.0 1 1282.0 2 259.0 3 248.5 4 201.0 5 212.0 6 350.5 7 672.5 8 1153.5 9 1463.5 10 1413.0 11 1322.0 12 1338.5 13 1482.5 14 1625.5 15 1726.5 16 1897.5 17 1976.5 18 2128.5 19 2307.0 20 2580.0 21 3090.0 22 3617.5 23 4371.0 24 5384.0 25 6792.0 26 8842.0 27 11269.0 28 13332.0 29 15651.5 30 18602.5 31 21731.0 32 25337.0 33 28714.0 34 32149.0 35 35364.0 36 39045.0 37 43025.5 38 45339.5 39 46536.0 40 47360.0 41 49114.0 42 50677.5 43 51785.5 44 53562.0 45 54707.0 46 54660.5 47 55199.0 48 56384.0 49 55660.0 50 53376.5 51 50480.0 52 47888.5 53 46140.5 54 44059.5 55 40494.0 56 36261.0 57 32558.5 58 29428.0 59 25892.5 60 21449.5 61 17276.5 62 13632.0 63 10242.0 64 7495.5 65 5551.5 66 4077.0 67 2986.5 68 2137.0 69 1479.5 70 1054.0 71 773.5 72 545.5 73 409.5 74 313.5 75 205.0 76 132.0 77 89.0 78 59.5 79 34.5 80 19.5 81 17.5 82 12.0 83 9.5 84 7.5 85 6.0 86 3.5 87 3.5 88 4.5 89 2.5 90 2.0 91 3.0 92 2.0 93 0.5 94 1.0 95 1.5 96 0.5 97 0.0 98 0.0 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.32846815269182317 2 0.2823265239060189 3 0.2788350950216332 4 0.2789035544115231 5 0.2801358234295416 6 0.24282545593953667 7 0.24255161837997702 8 0.24460540007667453 9 0.2571334684265294 10-11 0.2618913960238787 12-13 0.25388164740675834 14-15 0.24193548387096775 16-17 0.23909441919053617 18-19 0.2258817569417821 20-21 0.23129004874308562 22-23 0.2273878635193603 24-25 0.22985240155539732 26-27 0.22639520236595653 28-29 0.24515307519579385 30-31 0.2608302754805849 32-33 0.2517251766252259 34-35 0.2493975573689687 36-37 0.2347814776274714 38-39 0.2209526808697081 40-41 0.21756394107015717 42-43 0.2316323456925352 44-45 0.22927049674133304 46-47 0.24135357905690344 48-49 0.24874719316501454 50-51 0.262404841448053 52-53 0.2806834985486609 54-55 0.24833643682567502 56-57 0.21961772276685468 58-59 0.20685004655238512 60-61 0.21071800208116548 62-63 0.21640013144202858 64-65 0.20791116709567886 66-67 0.2128402431677529 68-69 0.21328522920203732 70-71 0.21513363272906513 72-73 0.216879347171258 74-75 0.2213634372090476 76-77 0.23437072128813188 78-79 0.23221425050659947 80-81 0.22526562243277287 82-83 0.21715318473081768 84-85 0.20325592858316446 86-87 0.20657620899282547 88-89 0.21972041185168958 90-91 0.21848814283367107 92-93 0.22146612629388246 94-95 0.21708472534092776 96-97 0.22519716304288295 98-99 0.23009200942001204 100-101 0.21112875842050496 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1460720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.204355690908855 #Duplication Level Percentage of deduplicated Percentage of total 1 79.55115989652691 49.48428645827925 2 12.001822874751527 14.931313180806605 3 3.758866543345759 7.0145361437081 4 1.6353944544375145 4.069146333550839 5 0.8235045915576182 2.561278626317335 6 0.49561546921504235 1.849766455978151 7 0.32222362713416597 1.4030599179987917 8 0.2262310803808623 1.12580468738798 9 0.17819468990481738 0.9976037285763418 >10 0.9249344700102693 10.302297183618474 >50 0.05395075742159684 2.318723079652238 >100 0.027330164080068713 2.7824285043490207 >500 3.3059195735821867E-4 0.15423194778048305 >1k 3.3059195735821867E-4 0.6411250700403813 >5k 1.1019731911940622E-4 0.36439868195602126 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5316 0.3639301166548004 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4039 0.276507475765376 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2761 0.18901637548606168 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2553 0.17477682238895886 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 6.84593898899173E-5 12-13 0.0 0.0 0.0 0.0 6.84593898899173E-5 14-15 0.0 0.0 0.0 0.0 1.0268908483487595E-4 16-17 0.0 0.0 0.0 0.0 1.369187797798346E-4 18-19 0.0 0.0 0.0 6.84593898899173E-5 1.369187797798346E-4 20-21 0.0 0.0 0.0 6.84593898899173E-5 1.7114847472479326E-4 22-23 0.0 0.0 0.0 1.369187797798346E-4 2.053781696697519E-4 24-25 0.0 0.0 0.0 1.369187797798346E-4 2.3960786461471053E-4 26-27 0.0 0.0 0.0 4.107563393395038E-4 2.738375595596692E-4 28-29 0.0 0.0 0.0 0.0014034174927433046 2.738375595596692E-4 30-31 0.0 0.0 0.0 0.0030122131551563614 2.738375595596692E-4 32-33 0.0 0.0 0.0 0.005510980886138343 2.738375595596692E-4 34-35 0.0 0.0 0.0 0.008317815871624952 2.738375595596692E-4 36-37 0.0 0.0 0.0 0.011672325976230899 2.738375595596692E-4 38-39 0.0 0.0 0.0 0.014924146996001971 2.738375595596692E-4 40-41 0.0 0.0 0.0 0.01673832082808478 2.738375595596692E-4 42-43 0.0 0.0 0.0 0.020743195136644944 2.738375595596692E-4 44-45 0.0 0.0 0.0 0.02430308341092064 3.0806725450462785E-4 46-47 0.0 0.0 0.0 0.030361739416178323 3.422969494495865E-4 48-49 0.0 0.0 0.0 0.03724190810011501 3.422969494495865E-4 50-51 0.0 0.0 0.0 0.044087847089106735 3.422969494495865E-4 52-53 0.0 0.0 0.0 0.057916643846870036 3.422969494495865E-4 54-55 0.0 0.0 0.0 0.08663535790569035 3.422969494495865E-4 56-57 0.0 0.0 0.0 0.12901172024754914 3.422969494495865E-4 58-59 0.0 0.0 0.0 0.1736130127608303 3.422969494495865E-4 60-61 0.0 0.0 0.0 0.21191604140423903 3.422969494495865E-4 62-63 0.0 0.0 0.0 0.2614464099895942 3.422969494495865E-4 64-65 0.0 0.0 0.0 0.32141683553316175 3.422969494495865E-4 66-67 0.0 0.0 0.0 0.42273673257023936 3.422969494495865E-4 68-69 0.0 0.0 0.0 0.5564721507201927 3.422969494495865E-4 70-71 0.0 0.0 0.0 0.6719631414644833 3.422969494495865E-4 72-73 0.0 0.0 0.0 0.8014198477463168 3.422969494495865E-4 74-75 0.0 0.0 0.0 0.9415904485459226 3.422969494495865E-4 76-77 0.0 0.0 0.0 1.1012377457692097 3.422969494495865E-4 78-79 0.0 0.0 0.0 1.2482542855578072 3.422969494495865E-4 80-81 0.0 0.0 0.0 1.4111191741059204 3.422969494495865E-4 82-83 0.0 0.0 0.0 1.5731283202804098 3.422969494495865E-4 84-85 0.0 0.0 0.0 1.8199928802234515 3.422969494495865E-4 86-87 0.0 0.0 0.0 2.1122802453584533 3.422969494495865E-4 88-89 0.0 3.422969494495865E-5 0.0 2.4761419026233638 3.422969494495865E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATCAA 6085 0.0 38.667015 1 GGTATCA 3560 0.0 38.186695 1 ATCAACG 7645 0.0 30.574545 3 TCAACGC 7665 0.0 30.496859 4 TATCAAC 7800 0.0 29.78527 2 CAACGCA 7825 0.0 29.752869 5 AACGCAG 8030 0.0 29.04649 6 GTACATG 5840 0.0 25.882742 1 CGCAGAG 9125 0.0 25.200819 8 ACGCAGA 9170 0.0 25.07715 7 TACATGG 5950 0.0 24.912937 2 ACATGGG 5920 0.0 23.91481 3 GCAGAGT 9645 0.0 23.201757 9 GAGTACT 5050 0.0 22.155767 12-13 AGAGTAC 8270 0.0 21.687334 10-11 AGTACTT 5390 0.0 21.507421 12-13 GTACTTT 5375 0.0 20.85566 14-15 CATGGGA 3220 0.0 19.919577 4 CATGGGG 2815 0.0 19.916168 4 ATGGGCG 195 1.0304248E-5 19.49272 5 >>END_MODULE