FastQCFastQC Report
Thu 26 May 2016
SRR937363_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937363_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1460720
Sequences flagged as poor quality0
Sequence length101
%GC44

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59950.41041404239005425No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48540.3323018785256586No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32790.22447833944903883No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA43800.040.0308951
GTATCAA66450.032.6071551
GTACATG57050.025.3198431
ATCAACG85550.025.0956213
TCAACGC85750.024.9816974
TATCAAC86150.024.9759752
CAACGCA86650.024.7222215
AACGCAG88250.024.274836
TACATGG59100.024.1108932
ACATGGG59950.022.4221253
TACCGTC1302.9406397E-421.9246187
GAGTACT58900.021.7753512-13
ACGCAGA99200.021.5010077
CGCAGAG99650.021.2132328
GTACTTT61000.020.98497614-15
AGTACTT61000.019.42931212-13
AGAGTAC90750.019.31540710-11
GCAGAGT107650.019.0631149
GTATTAG4250.019.0065381
ACTTTTT70300.018.27643416-17
Produced by FastQC (version 0.11.5)