##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937363_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1460720 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality warn #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.19720411851689 33.0 31.0 34.0 31.0 34.0 2 32.398438441316614 34.0 31.0 34.0 31.0 34.0 3 32.47016402869818 34.0 31.0 34.0 31.0 34.0 4 36.10582657867353 37.0 35.0 37.0 35.0 37.0 5 36.02973396681089 37.0 35.0 37.0 35.0 37.0 6 36.06718672983186 37.0 35.0 37.0 35.0 37.0 7 36.00490032312832 37.0 35.0 37.0 35.0 37.0 8 35.97903088887672 37.0 35.0 37.0 35.0 37.0 9 37.67322827098965 39.0 37.0 39.0 35.0 39.0 10-11 37.5893662029684 39.0 37.0 39.0 35.0 39.0 12-13 37.482142710444165 39.0 37.0 39.0 35.0 39.0 14-15 38.7055695136645 40.0 38.0 41.0 34.5 41.0 16-17 38.64650275206748 40.0 38.0 41.0 34.0 41.0 18-19 38.62996159428227 40.0 38.0 41.0 34.0 41.0 20-21 38.519009118790734 40.0 38.0 41.0 34.0 41.0 22-23 38.46694780656115 40.0 38.0 41.0 34.0 41.0 24-25 38.510111109589786 40.0 38.0 41.0 34.0 41.0 26-27 38.35439714661263 40.0 38.0 41.0 34.0 41.0 28-29 38.20910852182485 40.0 38.0 41.0 34.0 41.0 30-31 38.26037912810121 40.0 38.0 41.0 34.0 41.0 32-33 38.17377526151487 40.0 38.0 41.0 34.0 41.0 34-35 38.05337675940632 40.0 37.0 41.0 33.5 41.0 36-37 37.88663296182705 40.0 37.0 41.0 33.0 41.0 38-39 37.78457473027 40.0 37.0 41.0 33.0 41.0 40-41 37.60014547620352 40.0 37.0 41.0 33.0 41.0 42-43 37.406000465523846 40.0 37.0 41.0 32.5 41.0 44-45 37.116696560600246 40.0 36.5 41.0 31.5 41.0 46-47 36.79091920422805 39.5 36.0 41.0 31.0 41.0 48-49 36.47971856344816 39.0 36.0 41.0 30.0 41.0 50-51 36.24344432882414 39.0 35.5 40.5 30.0 41.0 52-53 36.009399816528834 39.0 35.0 40.0 29.5 41.0 54-55 35.794685839859795 39.0 35.0 40.0 29.0 41.0 56-57 35.48996111506654 38.0 35.0 40.0 28.0 41.0 58-59 35.22030573963525 38.0 34.0 40.0 28.0 41.0 60-61 34.87548435018347 38.0 34.0 40.0 27.0 41.0 62-63 34.49146824853497 37.5 34.0 40.0 26.0 41.0 64-65 34.069299386603866 37.0 33.0 39.5 26.0 41.0 66-67 33.62357844076894 36.0 33.0 39.0 25.5 40.0 68-69 33.165489279259546 36.0 32.0 39.0 24.0 40.0 70-71 32.72092084725341 35.0 32.0 38.0 23.0 40.0 72-73 32.22644620461142 35.0 31.0 37.5 22.0 39.5 74-75 31.72740566296073 35.0 31.0 37.0 20.5 39.0 76-77 30.420751410263435 33.5 29.5 35.5 20.0 38.0 78-79 31.067442425653105 34.0 31.0 36.0 20.0 38.0 80-81 30.888735349690563 34.0 31.0 36.0 19.5 37.5 82-83 30.46902281066871 34.0 30.0 35.0 18.0 37.0 84-85 30.011400884495316 34.0 30.0 35.0 14.0 36.5 86-87 29.618609658250726 34.0 29.5 35.0 7.5 36.0 88-89 29.14741291965606 34.0 29.0 35.0 4.5 36.0 90-91 28.558336642203844 33.5 29.0 35.0 2.0 35.0 92-93 28.034767443452544 33.0 27.5 35.0 2.0 35.0 94-95 27.50757297770962 33.0 26.5 35.0 2.0 35.0 96-97 26.79258105591763 32.5 25.5 34.0 2.0 35.0 98-99 25.76225662686894 32.0 24.0 34.0 2.0 35.0 100-101 22.759807834492577 29.0 9.5 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21342412413123668 1101 2 0.19003932015519354 1101 3 0.15653097510480762 1101 4 0.10153154269116982 1101 5 0.13079932152604812 1101 6 0.1265627409547605 1101 7 0.11980698554277325 1101 8 0.08125987120141787 1101 9 0.15833833298397337 1101 10-11 0.1981376266095296 1101 12-13 0.23537965571537 1101 14-15 0.23827825806413472 1101 16-17 0.22862996430721694 1101 18-19 0.2479980219149951 1101 20-21 0.20181505147727563 1101 22-23 0.12962749916854932 1101 24-25 0.16487653400472624 1101 26-27 0.18783314599841816 1101 28-29 0.11577515253728876 1101 30-31 0.2742377364915072 1101 32-33 0.27048315647663657 1101 34-35 0.42071514350575256 1101 36-37 0.40116723510595165 1101 38-39 0.2298665020127615 1101 40-41 0.18530620454616553 1101 42-43 0.3056900849044837 1101 44-45 0.3565048608330841 1101 46-47 0.41711202865025854 1101 48-49 0.3366043428189869 1101 50-51 0.3349110431960156 1101 52-53 0.4365552273965463 1101 54-55 0.43391222847717614 1101 56-57 0.5008875180821519 1101 58-59 0.5719070500382415 1101 60-61 0.6679243712482545 1101 62-63 0.7803907957564746 1101 64-65 0.7018765727844922 1101 66-67 0.5519250920330805 1101 68-69 0.7442739024644851 1101 70-71 0.7502406802726682 1101 72-73 0.7716241368861461 1101 74-75 0.8624278816596664 1101 76-77 0.8437836652877486 1101 78-79 0.9206762332559428 1101 80-81 0.9165989534035397 1101 82-83 0.8243737221948813 1101 84-85 1.1015423122240726 1101 86-87 1.0748368063168776 1101 88-89 1.2237612953021149 1101 90-91 1.3862708642545591 1101 92-93 1.5897400803154262 1101 94-95 1.8161624121062907 1101 96-97 2.010902464726808 1101 98-99 2.4493359093436986 1101 100-101 3.194146596165087 1102 1 0.1596503805246101 1102 2 0.2850391590905943 1102 3 0.21509943292915068 1102 4 0.09588927074970144 1102 5 0.09813148326354337 1102 6 0.07959666115977626 1102 7 0.12062054073960837 1102 8 0.09872177942322935 1102 9 0.2184995407537329 1102 10-11 0.13786761075361653 1102 12-13 0.047433730470821445 1102 14-15 0.263905425725234 1102 16-17 0.29994847492496035 1102 18-19 0.3483525429983487 1102 20-21 0.3671491173562771 1102 22-23 0.2414695055323861 1102 24-25 0.2696896811712364 1102 26-27 0.13012871027930117 1102 28-29 0.10618822955121487 1102 30-31 0.20124665947040654 1102 32-33 0.20810462603005675 1102 34-35 0.24430287539403395 1102 36-37 0.1881977765335634 1102 38-39 0.133191054342646 1102 40-41 0.10227632885224835 1102 42-43 0.16562633911453162 1102 44-45 0.06632604333358927 1102 46-47 -0.06784042150024305 1102 48-49 0.05450488363812411 1102 50-51 0.102994302857212 1102 52-53 -0.04798382361082787 1102 54-55 -0.11004212223562604 1102 56-57 -0.04583732273232499 1102 58-59 -0.10125708194883032 1102 60-61 -0.09938506248439438 1102 62-63 -0.08501521231107034 1102 64-65 0.010423197407327223 1102 66-67 0.08665044109586262 1102 68-69 -0.04394493779880548 1102 70-71 -0.04085191039776248 1102 72-73 0.19374224376861804 1102 74-75 0.040397823425863066 1102 76-77 0.009804045328625222 1102 78-79 -0.025483091847586792 1102 80-81 0.3374945799627369 1102 82-83 0.21857163837686144 1102 84-85 0.24778055898219975 1102 86-87 0.46256636811378726 1102 88-89 0.5426588260028176 1102 90-91 0.8565184742348357 1102 92-93 0.8591497964480119 1102 94-95 0.8305340275126554 1102 96-97 0.9851382131940092 1102 98-99 1.2733853597554834 1102 100-101 2.2264083726718447 1103 1 0.13932039610204328 1103 2 0.10313613935441168 1103 3 0.065952619158125 1103 4 0.043907792179396665 1103 5 0.029102000463034017 1103 6 0.009876197552387112 1103 7 -0.012865232344296373 1103 8 0.006581239500690117 1103 9 -0.017359290509759262 1103 10-11 -0.01895609267485554 1103 12-13 0.030647970249013667 1103 14-15 0.06835142632545654 1103 16-17 0.06538299967021999 1103 18-19 0.030759328472747427 1103 20-21 0.04960270659784527 1103 22-23 0.048615476121049994 1103 24-25 0.10638133366577307 1103 26-27 0.046046993045507634 1103 28-29 0.0683468478754321 1103 30-31 0.01313696001491138 1103 32-33 -0.021180240990084087 1103 34-35 0.0797894202041789 1103 36-37 0.16140529943525195 1103 38-39 0.23729370822342588 1103 40-41 0.007597309802044094 1103 42-43 -0.04579771016162226 1103 44-45 0.058234350823624936 1103 46-47 0.17419472062600505 1103 48-49 0.2441855461793878 1103 50-51 -0.008883135583332091 1103 52-53 0.01721168036927878 1103 54-55 -0.004689343306608862 1103 56-57 0.002695682855545556 1103 58-59 0.04158730088455087 1103 60-61 0.1171527449556109 1103 62-63 0.21340274890253852 1103 64-65 0.16412426782019907 1103 66-67 0.13397083610536953 1103 68-69 0.3253153483115483 1103 70-71 0.3699451113106136 1103 72-73 0.0816257845629309 1103 74-75 0.08008313061577965 1103 76-77 0.0816989190656372 1103 78-79 0.12508319800707213 1103 80-81 0.267496509278331 1103 82-83 0.35789966490137815 1103 84-85 0.39526673222044906 1103 86-87 0.4710131976051599 1103 88-89 0.80218678678769 1103 90-91 0.9875157874842309 1103 92-93 0.919146866746555 1103 94-95 0.580183535179895 1103 96-97 0.8587701855261933 1103 98-99 1.168510979637439 1103 100-101 1.6423105778081144 1104 1 0.1472764371561368 1104 2 0.15410875339395602 1104 3 0.10666490400959106 1104 4 0.03815275092794934 1104 5 0.024799384819147008 1104 6 -0.018298090680978873 1104 7 0.04011848714305444 1104 8 0.07981408618389452 1104 9 0.0877942648094816 1104 10-11 0.021894797308519287 1104 12-13 0.09963854155062535 1104 14-15 0.16037245597169658 1104 16-17 0.06983256159990958 1104 18-19 0.11866302257459438 1104 20-21 0.14344441492295346 1104 22-23 -0.014475059828264136 1104 24-25 -0.02782694530216645 1104 26-27 0.13913817499057046 1104 28-29 0.08148629493145876 1104 30-31 0.06645681025747052 1104 32-33 0.1618924697358608 1104 34-35 0.18144643479516276 1104 36-37 0.14760228696540167 1104 38-39 0.194388409780494 1104 40-41 0.06261715819890412 1104 42-43 0.14650936782213364 1104 44-45 0.08271723477581361 1104 46-47 0.05650618570177102 1104 48-49 0.26796440124775955 1104 50-51 0.13411938167043758 1104 52-53 0.175984480114856 1104 54-55 0.1441502069667635 1104 56-57 0.0990917364604087 1104 58-59 0.016775739974242754 1104 60-61 0.19990018617355787 1104 62-63 0.157214961487675 1104 64-65 -0.14735660289688468 1104 66-67 0.18261502590601708 1104 68-69 0.09904362404240885 1104 70-71 0.08268109497757337 1104 72-73 0.07362732785387038 1104 74-75 -0.07038560673938576 1104 76-77 0.033842702101932076 1104 78-79 -0.08811048429647883 1104 80-81 0.15640351996494317 1104 82-83 0.11398622265268799 1104 84-85 0.20411162083232526 1104 86-87 0.2019304121850709 1104 88-89 0.401439064455122 1104 90-91 0.4234495139994472 1104 92-93 0.5052043262937325 1104 94-95 0.5430105616543663 1104 96-97 0.8445749613514693 1104 98-99 1.3001587018280354 1104 100-101 2.0899393119829917 1105 1 0.0898810434590942 1105 2 0.08864968950904739 1105 3 0.03567130693262044 1105 4 0.031548130544898356 1105 5 0.0825626209880923 1105 6 0.03945564142410518 1105 7 0.043456525574676164 1105 8 -0.01928126773688632 1105 9 0.06662695456515877 1105 10-11 0.08819004281257037 1105 12-13 0.11728987616758246 1105 14-15 0.21528904901119716 1105 16-17 0.21162157842210405 1105 18-19 0.2011410488269334 1105 20-21 0.26811995674260203 1105 22-23 0.19911575150198502 1105 24-25 0.19295439434844042 1105 26-27 0.1770301736248001 1105 28-29 0.18523723213776577 1105 30-31 0.19725877724669516 1105 32-33 0.26218463169919204 1105 34-35 0.3110197375277579 1105 36-37 0.3953887200902386 1105 38-39 0.3447808924179796 1105 40-41 0.2608916426034398 1105 42-43 0.16257563578746215 1105 44-45 0.17577046809353192 1105 46-47 0.3673684506961976 1105 48-49 0.553725377561527 1105 50-51 0.47773476276579885 1105 52-53 0.40106482465477455 1105 54-55 0.42422071304056885 1105 56-57 0.4734749403155405 1105 58-59 0.536922771362299 1105 60-61 0.6270791688163158 1105 62-63 0.876618915882247 1105 64-65 0.897805828594656 1105 66-67 0.7331178612850735 1105 68-69 0.7273178620452967 1105 70-71 0.7071674355257755 1105 72-73 0.709408544303642 1105 74-75 0.525629726483654 1105 76-77 0.5255748705421013 1105 78-79 0.47865714838201256 1105 80-81 0.4854891234201979 1105 82-83 0.7074548187616543 1105 84-85 0.8372023072017392 1105 86-87 0.7976884316789636 1105 88-89 0.9699149646131708 1105 90-91 1.2120441093551264 1105 92-93 1.1720789676913235 1105 94-95 1.2888428536473704 1105 96-97 1.4827855221264414 1105 98-99 1.6944515114757124 1105 100-101 2.1661560961597814 1106 1 -0.17502827660157294 1106 2 0.040851001110524976 1106 3 -0.05488046287491244 1106 4 0.02518807386548616 1106 5 -0.03969971354861457 1106 6 -0.0827767868899727 1106 7 -0.1339144326349384 1106 8 -0.17710850945749002 1106 9 -0.24716479843792172 1106 10-11 -0.2732141967505086 1106 12-13 -0.4250829689822595 1106 14-15 -0.2016994655752029 1106 16-17 -0.269540705558839 1106 18-19 -0.21499585046742453 1106 20-21 -0.08595334198496829 1106 22-23 -0.12878853434452964 1106 24-25 -0.04151786763007692 1106 26-27 -0.04953758889131166 1106 28-29 0.14473388192877934 1106 30-31 0.05545593045912511 1106 32-33 -0.02442129685341854 1106 34-35 -0.3217022983739568 1106 36-37 0.06427414365492012 1106 38-39 0.073242359674218 1106 40-41 0.03192497380061354 1106 42-43 0.0931006324052035 1106 44-45 0.03167837116889416 1106 46-47 0.17963178210698771 1106 48-49 0.3566269932095949 1106 50-51 0.4117641278282065 1106 52-53 0.2559530679490152 1106 54-55 0.20387985330786762 1106 56-57 0.23878657027587735 1106 58-59 0.12725700900283954 1106 60-61 0.2634971153981027 1106 62-63 0.2784837824791353 1106 64-65 0.332943860053156 1106 66-67 0.26901387307666624 1106 68-69 0.4207520220720653 1106 70-71 0.4713857082278068 1106 72-73 0.438754025259783 1106 74-75 0.3887631865624037 1106 76-77 0.33582024397825805 1106 78-79 0.5224719006944056 1106 80-81 0.32591557191385334 1106 82-83 0.3174374446704107 1106 84-85 0.4663430729434488 1106 86-87 0.5861869968923266 1106 88-89 0.7922826829820018 1106 90-91 0.8046319944867513 1106 92-93 0.8099680156053672 1106 94-95 0.5286587167623118 1106 96-97 0.7082973101625285 1106 98-99 0.8948410044228297 1106 100-101 1.5645133900738664 1107 1 -6.292983546813957E-4 1107 2 0.048705513100856024 1107 3 0.006073173357762585 1107 4 -0.10404011407802471 1107 5 -0.10082034506312709 1107 6 0.004386866336083983 1107 7 -0.033687519732659155 1107 8 -0.03924207844768546 1107 9 0.04044766356706475 1107 10-11 -0.009842210818248986 1107 12-13 -0.020880385787435785 1107 14-15 -0.049747197557088896 1107 16-17 -0.007048311728055978 1107 18-19 -0.010301025386020513 1107 20-21 0.04861923251606726 1107 22-23 0.004521899850367106 1107 24-25 -0.059707094069231914 1107 26-27 -0.07885368229112544 1107 28-29 -0.13793519721050274 1107 30-31 -0.12642325382984865 1107 32-33 -0.05122959415376016 1107 34-35 -0.04669582999446931 1107 36-37 0.096711006179099 1107 38-39 0.004670549194514706 1107 40-41 0.008903455777669933 1107 42-43 -0.011574411260838247 1107 44-45 -0.04510487683916864 1107 46-47 0.15847068469712866 1107 48-49 0.10772536346465955 1107 50-51 0.003524451612705093 1107 52-53 0.03176691909673224 1107 54-55 0.06869932280758206 1107 56-57 0.06822579581913146 1107 58-59 0.12215612736809334 1107 60-61 0.22707476354549527 1107 62-63 0.2699417587973443 1107 64-65 0.40792438324292135 1107 66-67 0.3193565000916294 1107 68-69 0.3893905948286118 1107 70-71 0.49100854974211927 1107 72-73 0.4310199218846904 1107 74-75 0.297556169033637 1107 76-77 0.4259706400724319 1107 78-79 0.4725953195250234 1107 80-81 0.5459667957455814 1107 82-83 0.5431819175207764 1107 84-85 0.4654876700727044 1107 86-87 0.6817357125696013 1107 88-89 0.8809276130267456 1107 90-91 0.940096345294382 1107 92-93 0.9594074704983626 1107 94-95 1.086560747152589 1107 96-97 1.1199272414507746 1107 98-99 1.6284156208533034 1107 100-101 2.250874211690654 1108 1 -0.023150763031509314 1108 2 0.11043776804844896 1108 3 0.018980980996907704 1108 4 0.008231569525072757 1108 5 -0.01209296095965584 1108 6 -0.05638126422460488 1108 7 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0.7388455631395665 2316 76-77 0.7163658144941571 2316 78-79 0.8179294393132643 2316 80-81 0.5386343604875243 2316 82-83 0.6298441577158655 2316 84-85 0.8583174251639996 2316 86-87 0.9477627136201967 2316 88-89 0.8123261437278835 2316 90-91 1.0364174745175312 2316 92-93 1.3690467407568185 2316 94-95 1.5867070891147712 2316 96-97 1.506078307254814 2316 98-99 1.86688719728215 2316 100-101 2.1792970159860765 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 2.0 4 8.0 5 17.0 6 46.0 7 121.0 8 283.0 9 623.0 10 1055.0 11 1764.0 12 2702.0 13 3876.0 14 4998.0 15 7225.0 16 9673.0 17 13512.0 18 12263.0 19 7664.0 20 7033.0 21 7363.0 22 7724.0 23 8729.0 24 10321.0 25 12324.0 26 14782.0 27 18579.0 28 23132.0 29 28834.0 30 36415.0 31 46210.0 32 58491.0 33 76791.0 34 106228.0 35 150368.0 36 224431.0 37 316721.0 38 223282.0 39 17126.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.06981524007421 20.43262464292098 15.653261978027697 21.844298138977106 2 15.570061339613343 21.07631852784928 39.98459663727477 23.36902349526261 3 19.22942110739909 25.161427241360425 30.23180349416726 25.377348157073225 4 13.007489457253957 17.460088175694178 37.60433211019223 31.928090256859633 5 13.33428264922116 38.18036682387429 34.521438679099965 13.96391184780458 6 31.518360808368477 37.3608220603538 17.26888109973164 13.851936031546085 7 28.17740566296073 33.19424667287365 20.816172846267595 17.812174817898022 8 26.411632619530096 35.778177884878694 19.562749876773097 18.247439618818117 9 26.262048852620623 16.910085437318585 20.527000383372584 36.30086532668821 10-11 24.58722410865874 27.49476285667342 27.36989292951421 20.54812010515362 12-13 25.570048630000525 24.602343797603897 28.165143456105312 21.662464116290263 14-15 22.603373678733778 25.933991456268142 26.476737499315405 24.98589736568268 16-17 21.684374828851524 29.446437373350125 27.136891396023877 21.732296401774466 18-19 21.730584917027223 27.81566624678241 29.67361985869982 20.780128977490552 20-21 22.32179654115305 26.76307131150571 30.102798760609506 20.81233338673173 22-23 22.162160126869768 26.6541864387228 29.919412505078864 21.264240929328572 24-25 21.98704884375434 26.84342436841035 29.930944966143386 21.23858182169192 26-27 21.726806011675297 27.1202465134652 29.881679287405756 21.271268187453746 28-29 21.66398467550988 27.26159289393863 29.894496937532374 21.179925493019123 30-31 21.861984122854818 26.997753156671113 29.842351285260104 21.297911435213965 32-33 21.755849437006127 27.0569429064826 29.65635038944902 21.53085726706225 34-35 22.101326742976067 27.110055315187033 29.601737499315405 21.186880442521495 36-37 22.1452092118955 26.943767457144425 29.58849060737171 21.322532723588367 38-39 21.876718973081417 27.206558137958726 29.639639917177803 21.277082971782058 40-41 22.12391834163974 27.16574702886248 29.42771373021524 21.282620899282545 42-43 21.922398196628436 27.201128305368176 29.364421067405633 21.512052430597752 44-45 22.004080179637437 27.358391752012707 29.22240401993537 21.41512404841448 46-47 22.227155101593734 27.621378498274822 28.698005093378608 21.453461306752832 48-49 22.207575716085216 27.685867243551126 28.518401884002408 21.588155156361246 50-51 22.22835314091681 27.809573361082208 28.444260364751628 21.517813133249355 52-53 22.457870932783468 27.850350460851658 28.221263562237652 21.470515044127225 54-55 22.191316610986362 27.910071745440607 28.259248863574125 21.639362779998905 56-57 22.272338298921078 28.0309710279862 28.13537159756832 21.5613190755244 58-59 22.346787885426362 27.71123144750534 28.30586970808916 21.63611095897913 60-61 22.19521879621009 27.86605235774139 28.467194260364753 21.47153458568377 62-63 22.137473300837943 27.856091516512404 28.594049509830764 21.412385672818885 64-65 22.067233966810885 27.795265348595212 28.48797168519634 21.649528999397557 66-67 22.13716523358344 27.899220932143052 28.209684265293827 21.753929568979682 68-69 22.137439071142996 27.762507530532886 28.38565912700586 21.714394271318255 70-71 22.200490169231614 27.752683608083682 28.274823374774083 21.772002847910617 72-73 22.263666946438523 27.75029882646432 28.18984414471279 21.796190082384367 74-75 22.10978147762747 28.108740894901146 27.932663344104274 21.84881428336711 76-77 22.21688619311025 27.82816008543732 28.154505997042556 21.80044772440988 78-79 22.18638753491429 27.87337751245961 28.0414795443343 21.8987554082918 80-81 22.168211566898517 28.079337586943424 27.944917574894575 21.807533271263488 82-83 22.176354226889806 28.004670320360564 27.920362206551797 21.898613246197833 84-85 22.09989268972269 27.973684165487168 27.93466224646449 21.99176089832565 86-87 22.100368311517606 27.996570184566515 27.92876115888055 21.974300345035324 88-89 22.161365627909525 27.900145133906566 27.828639301166547 22.109849937017362 90-91 22.120180874156624 27.86966900330589 27.922935750922495 22.087214371614987 92-93 22.1013328185122 27.697993053337726 28.069253026713536 22.131421101436537 94-95 21.967112109096885 27.714414809135217 27.98729393723643 22.33117914453146 96-97 22.051864833780602 27.723177611041127 27.864169724519417 22.360787830658854 98-99 21.923414876760656 27.72225510186967 27.781678076037448 22.572651945332233 100-101 22.037462107401872 27.65123233319126 27.991619552323883 22.319686007082986 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 173.0 2 138.5 3 191.0 4 183.5 5 160.5 6 283.0 7 710.5 8 1243.5 9 1535.5 10 1483.0 11 1368.5 12 1445.5 13 1642.0 14 1834.0 15 1919.0 16 1963.5 17 2104.5 18 2282.0 19 2445.0 20 2719.0 21 3153.0 22 3732.0 23 4507.5 24 5468.0 25 6813.5 26 8858.5 27 11153.0 28 13248.0 29 15726.5 30 18621.0 31 21597.5 32 25004.0 33 28275.0 34 32111.5 35 35601.5 36 39357.0 37 43223.0 38 45631.0 39 47495.0 40 48426.5 41 49683.0 42 50782.0 43 51863.5 44 54045.5 45 55429.5 46 55110.5 47 55630.0 48 57081.0 49 56190.0 50 53795.0 51 50704.5 52 47733.0 53 46229.5 54 44669.5 55 41000.0 56 36354.5 57 32287.0 58 28961.5 59 25314.5 60 20774.0 61 16648.0 62 12952.5 63 9627.0 64 6835.0 65 4904.5 66 3527.5 67 2592.5 68 1864.5 69 1278.5 70 905.0 71 616.5 72 426.0 73 316.0 74 234.5 75 166.0 76 105.5 77 55.5 78 30.5 79 19.5 80 12.5 81 5.5 82 2.5 83 2.0 84 0.0 85 0.5 86 1.0 87 0.5 88 0.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04169176844295964 2 0.0 3 0.0 4 0.0 5 0.0013007284079084287 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006777479599101813 14-15 0.0 16-17 0.0 18-19 0.0 20-21 1.369187797798346E-4 22-23 6.503642039542144E-4 24-25 6.84593898899173E-5 26-27 0.0019853223068076018 28-29 0.003525658579330741 30-31 2.738375595596692E-4 32-33 1.7114847472479326E-4 34-35 0.0 36-37 0.0 38-39 3.422969494495865E-5 40-41 0.0 42-43 0.008386275261514869 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 3.422969494495865E-5 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 4.1075633933950384E-4 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 4.4498603428446245E-4 84-85 1.7114847472479326E-4 86-87 0.0 88-89 0.0 90-91 0.010029300618872885 92-93 0.00126649871296347 94-95 0.0 96-97 0.0 98-99 3.765266443945452E-4 100-101 0.01215154170546032 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1460720.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.04145446384297 #Duplication Level Percentage of deduplicated Percentage of total 1 77.80363948419998 46.714436772118475 2 13.067185571781643 15.691456549574267 3 3.9693178713962363 7.1497085468386565 4 1.777700878092482 4.269429852892936 5 0.9200045206367653 2.7619204766171013 6 0.5512945022863973 1.9860314253117417 7 0.3785847904685911 1.5911547020336412 8 0.2554422670147104 1.2269700194483641 9 0.18069377946981427 0.9764205598742881 >10 1.0054759071479815 10.857425209504308 >50 0.059857259561100475 2.4661405472098146 >100 0.029890513275536885 3.0759017830615685 >500 4.5632733440681606E-4 0.1862416903197036 >1k 3.4224550080511203E-4 0.636125994115148 >5k 1.1408183360170402E-4 0.41063587108003785 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5995 0.41041404239005425 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4854 0.3323018785256586 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3279 0.22447833944903883 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 6.84593898899173E-5 20-21 0.0 0.0 0.0 0.0 6.84593898899173E-5 22-23 0.0 0.0 0.0 6.84593898899173E-5 6.84593898899173E-5 24-25 0.0 0.0 0.0 6.84593898899173E-5 1.0268908483487595E-4 26-27 0.0 0.0 0.0 3.422969494495865E-4 1.369187797798346E-4 28-29 0.0 0.0 0.0 0.0014034174927433046 1.369187797798346E-4 30-31 0.0 0.0 0.0 0.0030806725450462785 1.369187797798346E-4 32-33 0.0 0.0 0.0 0.005579440276028261 2.053781696697519E-4 34-35 0.0 0.0 0.0 0.008386275261514869 2.053781696697519E-4 36-37 0.0 0.0 0.0 0.011740785366120818 2.053781696697519E-4 38-39 0.0 0.0 0.0 0.015163754860616682 2.053781696697519E-4 40-41 0.0 0.0 0.0 0.016909469302809572 2.053781696697519E-4 42-43 0.0 0.0 0.0 0.02081165452653486 2.053781696697519E-4 44-45 0.0 0.0 0.0 0.024508461580590394 2.053781696697519E-4 46-47 0.0 0.0 0.0 0.030977873925187582 2.053781696697519E-4 48-49 0.0 0.0 0.0 0.037926501999014185 2.053781696697519E-4 50-51 0.0 0.0 0.0 0.04466975190317104 2.053781696697519E-4 52-53 0.0 0.0 0.0 0.058430089271044414 2.738375595596692E-4 54-55 0.0 0.0 0.0 0.08735418149953447 3.422969494495865E-4 56-57 0.0 0.0 0.0 0.12969631414644833 3.422969494495865E-4 58-59 0.0 0.0 0.0 0.17474259269401393 3.422969494495865E-4 60-61 0.0 0.0 0.0 0.21321676981214743 3.422969494495865E-4 62-63 0.0 0.0 0.0 0.26308943534695217 3.422969494495865E-4 64-65 0.0 0.0 0.0 0.32357330631469416 3.422969494495865E-4 66-67 0.0 0.0 0.0 0.4258516348102306 3.422969494495865E-4 68-69 0.0 0.0 0.0 0.5603743359439181 3.422969494495865E-4 70-71 0.0 0.0 0.0 0.6768237581466674 3.422969494495865E-4 72-73 0.0 0.0 0.0 0.8077523413111343 3.422969494495865E-4 74-75 0.0 0.0 0.0 0.9507982364861165 3.422969494495865E-4 76-77 0.0 0.0 0.0 1.113355057779725 3.422969494495865E-4 78-79 0.0 0.0 0.0 1.264136864012268 3.422969494495865E-4 80-81 0.0 0.0 0.0 1.4294320609014732 3.422969494495865E-4 82-83 0.0 0.0 0.0 1.593529218467605 3.422969494495865E-4 84-85 0.0 0.0 0.0 1.8456993811271154 3.422969494495865E-4 86-87 0.0 0.0 0.0 2.14394271318254 4.7921572922942106E-4 88-89 0.0 0.0 0.0 2.5202982091023607 5.476751191193384E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4380 0.0 40.030895 1 GTATCAA 6645 0.0 32.607155 1 GTACATG 5705 0.0 25.319843 1 ATCAACG 8555 0.0 25.095621 3 TCAACGC 8575 0.0 24.981697 4 TATCAAC 8615 0.0 24.975975 2 CAACGCA 8665 0.0 24.722221 5 AACGCAG 8825 0.0 24.27483 6 TACATGG 5910 0.0 24.110893 2 ACATGGG 5995 0.0 22.422125 3 TACCGTC 130 2.9406397E-4 21.924618 7 GAGTACT 5890 0.0 21.77535 12-13 ACGCAGA 9920 0.0 21.501007 7 CGCAGAG 9965 0.0 21.213232 8 GTACTTT 6100 0.0 20.984976 14-15 AGTACTT 6100 0.0 19.429312 12-13 AGAGTAC 9075 0.0 19.315407 10-11 GCAGAGT 10765 0.0 19.063114 9 GTATTAG 425 0.0 19.006538 1 ACTTTTT 7030 0.0 18.276434 16-17 >>END_MODULE