FastQCFastQC Report
Thu 26 May 2016
SRR937362_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937362_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424932
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53560.37587758573742464No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39520.2773465681169347No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT27880.1956584594914003No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN21400.1501826052050203No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36350.039.2659951
GTATCAA62250.038.902561
TCAACGC77700.030.8370554
CAACGCA78100.030.7389095
ATCAACG78350.030.6408253
TATCAAC78550.030.6244092
AACGCAG80600.029.7024426
ACGCAGA89150.026.7472467
CGCAGAG89850.026.434068
GTACATG58300.025.6248231
TACATGG59200.024.5732782
GCAGAGT94600.024.453999
ACATGGG58350.023.3009283
GAGTACT51750.022.71755212-13
AGTACTT54500.021.83272412-13
GTACTTT53750.021.73892414-15
AGAGTAC83600.021.4213610-11
CAGAGTA92900.019.35361510-11
CATGGGA29100.019.2775754
TACTTTT62600.018.85529914-15
Produced by FastQC (version 0.11.5)