##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937362_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1424932 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.508245305740907 31.0 31.0 34.0 30.0 34.0 2 31.62816401063349 33.0 31.0 34.0 30.0 34.0 3 31.659275670698673 33.0 31.0 34.0 30.0 34.0 4 35.2853146676473 37.0 35.0 37.0 33.0 37.0 5 35.28678421145711 37.0 35.0 37.0 33.0 37.0 6 35.322976113947895 37.0 35.0 37.0 33.0 37.0 7 35.25560237260445 37.0 35.0 37.0 33.0 37.0 8 35.24283825473777 37.0 35.0 37.0 32.0 37.0 9 36.832776581619335 39.0 37.0 39.0 33.0 39.0 10-11 36.70246194204355 39.0 37.0 39.0 32.0 39.0 12-13 36.55796802935158 39.0 37.0 39.0 32.0 39.0 14-15 37.69940600674278 40.0 37.0 41.0 32.0 41.0 16-17 37.64896079251501 40.0 37.0 41.0 32.0 41.0 18-19 37.61466862980128 40.0 37.0 41.0 32.0 41.0 20-21 37.54987395889769 40.0 37.0 41.0 32.0 41.0 22-23 37.39255241653637 40.0 37.0 41.0 32.0 41.0 24-25 37.233173583020104 40.0 37.0 41.0 31.0 41.0 26-27 37.06369005678867 39.0 36.5 41.0 31.0 41.0 28-29 37.127838731953524 39.0 37.0 41.0 31.0 41.0 30-31 36.97241692936926 39.0 36.5 41.0 31.0 41.0 32-33 36.77753675263101 39.0 36.0 41.0 30.0 41.0 34-35 36.65621798092821 39.0 36.0 41.0 30.0 41.0 36-37 36.652697462054334 39.0 36.0 41.0 30.0 41.0 38-39 36.563579876092334 39.0 36.0 41.0 30.0 41.0 40-41 36.3838814764494 39.0 35.5 41.0 30.0 41.0 42-43 36.14697753998085 39.0 35.0 41.0 29.5 41.0 44-45 35.9252241510472 39.0 35.0 41.0 28.5 41.0 46-47 35.60454393613169 39.0 35.0 40.5 27.0 41.0 48-49 35.29509478347037 39.0 35.0 40.0 26.0 41.0 50-51 34.43249397164216 38.0 34.0 39.5 25.0 40.5 52-53 34.53569468578149 38.0 34.0 39.5 24.5 40.5 54-55 34.86141057959257 38.0 34.0 40.0 25.0 41.0 56-57 34.66042800638908 38.0 34.0 40.0 24.0 41.0 58-59 34.406402200245346 38.0 34.0 40.0 23.0 41.0 60-61 34.1154560357968 38.0 34.0 40.0 23.0 41.0 62-63 33.779870899102555 37.5 33.0 40.0 22.0 41.0 64-65 33.40374137151808 37.0 33.0 40.0 21.5 41.0 66-67 33.02502119399382 36.5 32.5 39.0 20.0 41.0 68-69 32.54236377595562 36.0 32.0 39.0 18.0 40.5 70-71 32.09673935317615 35.0 31.0 39.0 16.0 40.0 72-73 31.697030454786614 35.0 31.0 38.0 13.0 40.0 74-75 31.229021805952847 35.0 31.0 37.0 9.0 39.0 76-77 30.78144851824508 35.0 30.5 37.0 7.0 39.0 78-79 30.291309339673752 34.0 30.0 36.0 4.5 39.0 80-81 29.794560722897657 34.0 29.5 36.0 2.0 37.5 82-83 29.291258810946765 34.0 29.0 35.0 2.0 37.0 84-85 28.834145418869113 34.0 29.0 35.0 2.0 36.5 86-87 28.380704833634166 34.0 28.0 35.0 2.0 36.0 88-89 27.95079344137124 34.0 27.0 35.0 2.0 36.0 90-91 27.497217761970397 33.0 26.0 35.0 2.0 35.0 92-93 27.014573677901822 33.0 25.5 35.0 2.0 35.0 94-95 26.45412412662499 33.0 25.0 35.0 2.0 35.0 96-97 25.772006313283722 32.0 23.5 34.5 2.0 35.0 98-99 24.95586842038778 32.0 19.0 34.0 2.0 35.0 100-101 23.001553758354785 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.05958917139735931 1101 2 0.0882305439161648 1101 3 0.03203099244849028 1101 4 0.09804260698751222 1101 5 0.04906052344711043 1101 6 0.04322775530500422 1101 7 -0.05835009129875601 1101 8 0.08769094723286486 1101 9 0.07618625695844372 1101 10-11 0.1333364800658785 1101 12-13 0.030836430920558655 1101 14-15 0.14111067957251322 1101 16-17 0.20863552738704527 1101 18-19 0.30340273223205827 1101 20-21 0.22535004328682362 1101 22-23 0.3169345334482898 1101 24-25 0.31472543388865404 1101 26-27 0.12321831012053508 1101 28-29 0.15399622100888877 1101 30-31 0.12647604481396968 1101 32-33 0.2558031139637009 1101 34-35 0.1367833071795559 1101 36-37 0.19583659794525943 1101 38-39 0.0497013839851661 1101 40-41 0.02511315295675587 1101 42-43 0.1963795323768096 1101 44-45 0.22325997396279718 1101 46-47 -0.017752817349702354 1101 48-49 0.22564377617525366 1101 50-51 0.15118540653529067 1101 52-53 0.1630560726635153 1101 54-55 0.2846168390255528 1101 56-57 0.163074331743168 1101 58-59 0.38172121448256746 1101 60-61 0.28393029596052344 1101 62-63 0.2527192561509253 1101 64-65 0.06322881406387637 1101 66-67 -0.03295273592389236 1101 68-69 0.22896237026971988 1101 70-71 0.07892334379746302 1101 72-73 0.07596421026641664 1101 74-75 0.03508954289997135 1101 76-77 0.17801967993132806 1101 78-79 0.10260439790345544 1101 80-81 0.18039761014752287 1101 82-83 0.31096659268738236 1101 84-85 0.282146167636558 1101 86-87 0.15345316527831443 1101 88-89 0.16125979298692883 1101 90-91 0.3571257220435804 1101 92-93 0.35517368790258885 1101 94-95 0.4204440590701246 1101 96-97 0.4901628142065739 1101 98-99 0.5760471055533181 1101 100-101 1.2578922839992153 1102 1 0.05759036037986931 1102 2 -0.038983774099627055 1102 3 0.04969161790506149 1102 4 0.068426299198876 1102 5 0.04079287812759702 1102 6 -0.056322806492751454 1102 7 -0.01877622272116497 1102 8 0.005430885313359113 1102 9 -0.006726394796523039 1102 10-11 0.14285364707461667 1102 12-13 0.004553340511122883 1102 14-15 0.14241446779389122 1102 16-17 0.22337480452900849 1102 18-19 0.26462651210474064 1102 20-21 0.22092662519101935 1102 22-23 0.24158040953970783 1102 24-25 0.09702202135865434 1102 26-27 0.11126227992608761 1102 28-29 0.18480273994999408 1102 30-31 0.09877715449498226 1102 32-33 -0.013654046783507567 1102 34-35 -0.018384884283904057 1102 36-37 0.12224839743738158 1102 38-39 0.20909732095250178 1102 40-41 0.11446595498827605 1102 42-43 0.09371595008996536 1102 44-45 0.04366332039894871 1102 46-47 0.05596739693352504 1102 48-49 0.062286556291439865 1102 50-51 -0.03215789885309306 1102 52-53 -0.09858235917285896 1102 54-55 -0.04241728627447827 1102 56-57 -0.008109270023972215 1102 58-59 -0.07243626281941573 1102 60-61 -0.06709868097620131 1102 62-63 -0.19748406941348406 1102 64-65 -0.02222149379433347 1102 66-67 0.21261375797112692 1102 68-69 -0.13166569526755012 1102 70-71 0.01415919449492975 1102 72-73 -0.20860774506805413 1102 74-75 0.13665961977755714 1102 76-77 0.19527278460311592 1102 78-79 0.11736089423350649 1102 80-81 0.18714701008490664 1102 82-83 0.11786105123957569 1102 84-85 0.27730566254037115 1102 86-87 0.11968772888495138 1102 88-89 0.16597506762428083 1102 90-91 0.40349229942556875 1102 92-93 0.39687351745001465 1102 94-95 0.5545938833996225 1102 96-97 0.6532961846452281 1102 98-99 0.7087729552071984 1102 100-101 1.1828985455221535 1103 1 0.005931358389339891 1103 2 -0.04654709347638786 1103 3 -0.013636681909616755 1103 4 -0.0423047049880978 1103 5 -0.16844660296238345 1103 6 -0.088784883505582 1103 7 -0.08484184150076857 1103 8 0.03211150855448608 1103 9 -0.015015913637896006 1103 10-11 0.06962075531814094 1103 12-13 0.08057914873406702 1103 14-15 0.08007594078448932 1103 16-17 -0.102928991201658 1103 18-19 -0.07800853491290383 1103 20-21 -0.06383416827456045 1103 22-23 -0.1997480961916338 1103 24-25 -0.19066827996607572 1103 26-27 0.09673829063375194 1103 28-29 0.0732257614339673 1103 30-31 -0.0010052602211985118 1103 32-33 0.015314653882292362 1103 34-35 0.12083125958321261 1103 36-37 0.019556380140379304 1103 38-39 -0.039765675821470836 1103 40-41 0.21360625288105695 1103 42-43 6.615194495651622E-4 1103 44-45 0.07443791028158131 1103 46-47 0.06541588344597926 1103 48-49 0.01721208309492539 1103 50-51 -0.01740808761004331 1103 52-53 0.029284344101220938 1103 54-55 -0.07532963441093443 1103 56-57 -0.0197844021538387 1103 58-59 -0.10648424140407542 1103 60-61 -0.15265294001937235 1103 62-63 -0.17616380034903045 1103 64-65 0.011231728801583074 1103 66-67 -0.029928626543942016 1103 68-69 -0.05125008678378862 1103 70-71 0.19892994059966185 1103 72-73 0.18417350278697953 1103 74-75 0.050446084188290996 1103 76-77 0.24868585344792749 1103 78-79 0.27883148246880296 1103 80-81 0.2258988118557923 1103 82-83 0.2630003881878942 1103 84-85 0.25175826738627904 1103 86-87 0.40842992121112687 1103 88-89 0.435204252880645 1103 90-91 0.638302512892519 1103 92-93 0.5223889955198935 1103 94-95 0.6185354717035558 1103 96-97 0.7951683103916771 1103 98-99 0.9956361578978417 1103 100-101 1.1674764163437636 1104 1 0.17168779316741833 1104 2 0.10639659110487187 1104 3 0.1061857894568412 1104 4 0.19686373602927887 1104 5 0.2096164075393645 1104 6 0.10463939281996204 1104 7 0.13779072020720662 1104 8 0.08576172555323325 1104 9 0.27916876051646256 1104 10-11 0.21596058056965717 1104 12-13 0.3328620798144044 1104 14-15 0.485033563542153 1104 16-17 0.35698237814263933 1104 18-19 0.4329002133378097 1104 20-21 0.3775016538279061 1104 22-23 0.3127592384595985 1104 24-25 0.4295103981526651 1104 26-27 0.30987423329849406 1104 28-29 0.4148724791858953 1104 30-31 0.24898805672528823 1104 32-33 0.13153529355744098 1104 34-35 0.33296340761017973 1104 36-37 0.33078016753373873 1104 38-39 0.2629201765552196 1104 40-41 0.21529305558796352 1104 42-43 0.35992510041675985 1104 44-45 0.3266526742160778 1104 46-47 0.38942167898999 1104 48-49 0.3632662746392654 1104 50-51 0.29344078048117694 1104 52-53 0.24933624469480975 1104 54-55 0.2798318883094453 1104 56-57 0.44752343666694827 1104 58-59 0.31234205400559745 1104 60-61 0.32238585510627615 1104 62-63 0.1510258018889843 1104 64-65 0.23332530224366366 1104 66-67 0.451772170477021 1104 68-69 0.48375274014784253 1104 70-71 0.48515676878236746 1104 72-73 0.7183863927932741 1104 74-75 0.5467226209230311 1104 76-77 0.4985087525298084 1104 78-79 0.6731613793760189 1104 80-81 0.6583197747234095 1104 82-83 0.5803207911492869 1104 84-85 0.8180003596632055 1104 86-87 0.9132808156041783 1104 88-89 0.6744886448764866 1104 90-91 0.6450393649871877 1104 92-93 0.9253934958881267 1104 94-95 1.0170145423848673 1104 96-97 1.1135222475789952 1104 98-99 1.2132093139431284 1104 100-101 1.1075858981264268 1105 1 -0.10158200624119829 1105 2 0.039867115093258576 1105 3 -0.016956808907526266 1105 4 0.042933823138682214 1105 5 -0.023523127344354577 1105 6 -0.047136759981121656 1105 7 0.11632637645804067 1105 8 0.1602735665719095 1105 9 0.1473365327000593 1105 10-11 0.10683107525518665 1105 12-13 -0.09282354047383023 1105 14-15 0.027756465057912294 1105 16-17 0.05021474980944873 1105 18-19 0.03071748337696789 1105 20-21 0.1035429042607845 1105 22-23 0.11509614141385072 1105 24-25 0.08431340302759338 1105 26-27 0.17015420879065601 1105 28-29 0.07297305458747871 1105 30-31 -0.038288508979711366 1105 32-33 -0.1675972752376751 1105 34-35 0.12668693514154938 1105 36-37 -0.02851083648874919 1105 38-39 0.08970265610981443 1105 40-41 -0.14552036512116473 1105 42-43 0.07972484201882679 1105 44-45 0.209234455829872 1105 46-47 0.2516855911879716 1105 48-49 0.31688983946516913 1105 50-51 0.02264374273377001 1105 52-53 0.1353574493086782 1105 54-55 0.1159616466940534 1105 56-57 0.09441466978450563 1105 58-59 0.22552201351437873 1105 60-61 0.2734827143726406 1105 62-63 0.2492293368792673 1105 64-65 0.41896523671181995 1105 66-67 0.29960076207509445 1105 68-69 0.13787944837129373 1105 70-71 0.21000392773705556 1105 72-73 0.1793300114298937 1105 74-75 0.06189217791709112 1105 76-77 -0.01868070376897535 1105 78-79 0.20208729642229173 1105 80-81 0.2598385285527556 1105 82-83 0.28922220035038393 1105 84-85 0.12822905515323058 1105 86-87 0.08451673025293971 1105 88-89 -0.07155913210212006 1105 90-91 0.08357095879629028 1105 92-93 0.3337784422106189 1105 94-95 0.3490672076130288 1105 96-97 0.4423898918955693 1105 98-99 0.5017825311305906 1105 100-101 0.73843548122667 1106 1 -0.06739821757558317 1106 2 -0.11205487632692623 1106 3 -0.20731580642389602 1106 4 -0.07671790411351509 1106 5 -0.15688844682956926 1106 6 -0.06996358319177887 1106 7 -0.1389090039890135 1106 8 -0.17227328373289907 1106 9 -0.022727251449857988 1106 10-11 -0.6084855876484028 1106 12-13 -0.5345428902141691 1106 14-15 -0.5361956121604194 1106 16-17 -0.5689360178581992 1106 18-19 -0.29621015265825434 1106 20-21 -0.24876997675072943 1106 22-23 -0.28096956190518085 1106 24-25 -0.2748026502575698 1106 26-27 -0.27767261442000546 1106 28-29 -0.08103321093521032 1106 30-31 -0.15367572957126896 1106 32-33 -0.12959208219184148 1106 34-35 -0.21975695738498757 1106 36-37 -0.1643442638990038 1106 38-39 -0.3492564768773079 1106 40-41 -0.3138964093749479 1106 42-43 -0.2865824508238006 1106 44-45 -0.0850588383674662 1106 46-47 -0.024525178737519582 1106 48-49 0.047307258747174785 1106 50-51 0.02702114169516534 1106 52-53 -0.21064475209119138 1106 54-55 -0.0460402160289064 1106 56-57 -0.17521306785378243 1106 58-59 -0.07800022626886971 1106 60-61 -0.36532683631600804 1106 62-63 -0.20039811852827683 1106 64-65 -0.04903402948822588 1106 66-67 0.024260674019096484 1106 68-69 -0.34514013844288627 1106 70-71 -0.21157966901164116 1106 72-73 0.05167280890110604 1106 74-75 0.023710590651344177 1106 76-77 0.1628698719817585 1106 78-79 0.08587031742025886 1106 80-81 0.1699889575435023 1106 82-83 -0.015286435910621066 1106 84-85 -0.019071407705201437 1106 86-87 0.10408378827958487 1106 88-89 0.16091010474592338 1106 90-91 0.18685894705549444 1106 92-93 0.44836694977440317 1106 94-95 0.5289356880826581 1106 96-97 0.6143841343882315 1106 98-99 0.5664557079199319 1106 100-101 0.6729099675666994 1107 1 -0.022826089385876713 1107 2 -0.03435597607776586 1107 3 -0.1052294301601826 1107 4 -0.007259975310930145 1107 5 -0.05085556886181308 1107 6 0.09134681002642253 1107 7 0.037952911410357615 1107 8 0.12234395142476018 1107 9 0.17734613687101586 1107 10-11 0.21548769184295935 1107 12-13 0.23596753450731 1107 14-15 0.08846340064136626 1107 16-17 0.1624360573487209 1107 18-19 0.14321405331591563 1107 20-21 -0.05335066094467322 1107 22-23 0.16518703803181012 1107 24-25 0.2329893994603367 1107 26-27 0.2694784867628002 1107 28-29 0.3230545185375391 1107 30-31 0.1679147564384138 1107 32-33 0.16787727370929417 1107 34-35 0.30757034736627986 1107 36-37 0.16123156592600907 1107 38-39 0.2710971474317674 1107 40-41 0.2835922780838871 1107 42-43 0.1452741662968009 1107 44-45 0.03366592320999473 1107 46-47 -0.0732560977740917 1107 48-49 0.05875887973409277 1107 50-51 -0.13440846279549135 1107 52-53 0.09605237967844005 1107 54-55 -0.030678608502491045 1107 56-57 -0.15233363233527086 1107 58-59 -0.20053187274480422 1107 60-61 -0.06765915295260072 1107 62-63 -0.17172404859099544 1107 64-65 -0.18938095935046562 1107 66-67 -0.07235934631483332 1107 68-69 -0.01759750161841822 1107 70-71 0.06123103207951175 1107 72-73 -0.05576894583154868 1107 74-75 0.09390150768316019 1107 76-77 0.04047927427863485 1107 78-79 0.17263679220331696 1107 80-81 0.17416332103406518 1107 82-83 0.21472813435601168 1107 84-85 0.1974253918480251 1107 86-87 0.17691399992434143 1107 88-89 0.05595424513599312 1107 90-91 0.3937230820040831 1107 92-93 0.4109625335874476 1107 94-95 0.2394030105903191 1107 96-97 0.41830486068201367 1107 98-99 0.5348826203121781 1107 100-101 0.4847387329245514 1108 1 -0.061595530224181516 1108 2 -0.0397184665739303 1108 3 -0.07976449651031459 1108 4 0.0399249068242753 1108 5 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54-55 -0.7814444355957519 2316 56-57 -1.2448966003407165 2316 58-59 -1.348665523754562 2316 60-61 -1.2085089373864548 2316 62-63 -1.1782080513139377 2316 64-65 -1.16990626000851 2316 66-67 -1.366760299344108 2316 68-69 -1.2751423166853542 2316 70-71 -1.6835783620661608 2316 72-73 -1.6036529939368336 2316 74-75 -1.5875711494532112 2316 76-77 -1.747675933345601 2316 78-79 -1.6275787739414511 2316 80-81 -1.7229208632182633 2316 82-83 -1.8816185867694735 2316 84-85 -1.902897957369131 2316 86-87 -1.986428409878247 2316 88-89 -2.1395302114104098 2316 90-91 -2.3937173536664353 2316 92-93 -2.4004567992920656 2316 94-95 -2.9745463880935894 2316 96-97 -2.726311658492026 2316 98-99 -3.3475317205092416 2316 100-101 -3.8415660095306343 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6318.0 3 1459.0 4 1328.0 5 1427.0 6 1556.0 7 1821.0 8 2326.0 9 2873.0 10 3618.0 11 4399.0 12 5648.0 13 6980.0 14 7860.0 15 9530.0 16 11557.0 17 14272.0 18 12415.0 19 9304.0 20 9500.0 21 9862.0 22 11066.0 23 12238.0 24 13593.0 25 15478.0 26 17598.0 27 20900.0 28 24795.0 29 29790.0 30 35934.0 31 44369.0 32 56121.0 33 72053.0 34 96671.0 35 134263.0 36 195099.0 37 270946.0 38 220284.0 39 29678.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.41895323324212 19.55596409113161 15.290359616962679 22.734723058663594 2 14.699768036973287 20.149244800191486 41.17121968904986 23.979767473785362 3 18.723746945723097 24.952183979574095 30.047467563596893 26.27660151110592 4 12.222594552279364 16.69191627642409 38.17008850504476 32.915400666251784 5 13.170377650182896 37.798528372339256 34.70616726318369 14.324926714294161 6 31.154523035455284 36.848991121758935 17.854605613929454 14.141880228856325 7 27.943018500349893 32.871123489220146 21.184219317586408 18.001638692843557 8 26.032043225085406 35.29607875270702 19.997369470525058 18.67450855168252 9 25.998188308966462 16.82615080358625 20.72677223611623 36.44888865133106 10-11 24.297291308339226 27.107317090328415 27.778035714913806 20.817355886418554 12-13 25.251139111584404 24.30826842378468 28.424614657436546 22.01597780719437 14-15 22.353109563923248 25.65507166665143 26.669883266949924 25.3219355024754 16-17 21.539448928276276 29.08839302983946 27.304460973079426 22.06769706880484 18-19 21.507070712754008 27.547788059367683 29.82489023111423 21.120250996764078 20-21 22.218998963751705 26.542584108585597 29.954713804536215 21.283703123126482 22-23 21.909713907557695 26.536822521134045 29.795057511399076 21.758406059909188 24-25 21.646398812443326 26.634586884910444 29.930780944384615 21.788233358261618 26-27 21.435997759593718 26.904354130459012 29.833864662161712 21.82578344778556 28-29 21.448724319256556 26.98304519698314 29.934917441471587 21.63331304228872 30-31 21.59075601849556 26.815185435678618 29.811411269461257 21.78264727636456 32-33 21.404229788299574 26.8298181224793 29.74349487876004 22.022457210461088 34-35 21.793140706230595 26.894613388745487 29.656029395264948 21.65621650975897 36-37 21.733000046062 26.7057619340132 29.674615936115174 21.88662208380963 38-39 21.52913246067923 26.96484301930631 29.6938443990318 21.812180120982656 40-41 21.76545313841029 26.93476495984186 29.59589414130362 21.703887760444225 42-43 21.5149921094161 26.88125726116567 29.441793933392784 22.161956696025452 44-45 21.64894183773474 27.09069816590834 29.30776991375124 21.952590082605685 46-47 21.864859951055223 27.247659931221303 28.866410443333884 22.021069674389587 48-49 21.829219785124124 27.330433293275785 28.775916514296746 22.064430407303345 50-51 21.90622971068982 27.506448615420542 28.642896182743243 21.944425491146387 52-53 21.998781381021768 27.522143756231177 28.463301767912185 22.01577309483487 54-55 21.817544294021282 27.486173175221374 28.542027005830374 22.15425552492697 56-57 21.87158850831169 27.717388820309907 28.33175177823867 22.07927089313973 58-59 21.94234069400853 27.362662823603618 28.522472812778304 22.172523669609554 60-61 21.83834740973274 27.560339605513935 28.68407258014899 21.91724040460434 62-63 21.794366024847207 27.471658892683877 28.865209475609525 21.868765606859387 64-65 21.70992378220816 27.393544181814537 28.769570822708463 22.126961213268835 66-67 21.716843759877005 27.475505025912696 28.50624692593305 22.301404288277254 68-69 21.69064332152225 27.382744894275028 28.756165839867283 22.170445944335444 70-71 21.760371168483868 27.337285928907423 28.620350065941054 22.281992836667655 72-73 21.70298210497929 27.4087713271821 28.559780802960695 22.328465764877915 74-75 21.67286850543011 27.68763829315177 28.322191354649657 22.317301846768466 76-77 21.732347254263463 27.436408905654062 28.44991422265401 22.381329617428467 78-79 21.750982845863117 27.47583804289466 28.34769730492254 22.425481806319677 80-81 21.725443396667593 27.67745975666058 28.232661547370945 22.36443529930088 82-83 21.708325437310737 27.618827961869858 28.353395383472463 22.319451217346945 84-85 21.74069104103335 27.571400296221714 28.22494428704468 22.462964375700256 86-87 21.72454962667231 27.5911627615913 28.332755593587162 22.351532018149232 88-89 21.817984937959878 27.361499158512835 28.218103909865423 22.602411993661864 90-91 21.703341617968505 27.35423864741875 28.36622048708592 22.576199247526826 92-93 21.770082936930667 27.123997567089614 28.509244707892545 22.596674788087174 94-95 21.591160780392727 27.195443419980354 28.42346437614215 22.789931423484767 96-97 21.631571823578323 27.174324602249655 28.348371469202142 22.84573210496988 98-99 21.490277208381297 27.188129144044144 28.246022877901318 23.075570769673245 100-101 21.504423005464115 26.95387931837589 28.6748139166739 22.866883759486093 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1996.0 1 1114.5 2 245.5 3 277.0 4 258.5 5 233.5 6 347.5 7 707.5 8 1228.5 9 1531.0 10 1429.0 11 1299.0 12 1352.0 13 1456.0 14 1570.5 15 1713.0 16 1871.0 17 2002.5 18 2186.0 19 2377.0 20 2577.5 21 2990.0 22 3590.5 23 4293.0 24 5240.0 25 6662.0 26 8713.5 27 11016.0 28 12987.0 29 15256.5 30 17991.0 31 20994.5 32 24527.5 33 27682.5 34 30949.0 35 34164.0 36 38016.0 37 41856.5 38 44255.0 39 45835.0 40 46522.0 41 47804.0 42 49085.5 43 50336.0 44 52119.5 45 53334.0 46 53652.0 47 54215.0 48 55743.5 49 55043.5 50 52112.0 51 49298.0 52 46910.5 53 45162.0 54 43389.5 55 40001.0 56 35521.5 57 31841.5 58 28846.0 59 25153.5 60 20789.0 61 16808.0 62 13019.5 63 9616.0 64 6994.5 65 5146.0 66 3660.0 67 2614.5 68 1867.5 69 1306.5 70 944.5 71 629.0 72 454.5 73 349.0 74 252.5 75 167.0 76 117.5 77 78.5 78 46.5 79 30.5 80 22.5 81 17.0 82 13.5 83 9.5 84 8.0 85 9.5 86 9.0 87 8.5 88 6.5 89 4.5 90 4.5 91 4.0 92 5.0 93 4.5 94 3.0 95 3.0 96 6.0 97 7.0 98 5.0 99 4.0 100 1.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.36682452215263606 2 0.3120850679190305 3 0.3075936255203757 4 0.3121552467065095 5 0.31327810730617317 6 0.22836177445660563 7 0.21523834119803611 8 0.2221158623709763 9 0.21446637453576733 10-11 0.23839734106610003 12-13 0.22527390780753045 14-15 0.22043157147148074 16-17 0.22155443207114447 18-19 0.21622084422274185 20-21 0.20881698214370933 22-23 0.21029073668076792 24-25 0.20018499128379458 26-27 0.20130785188345832 28-29 0.21913326390311957 30-31 0.2311338365620254 32-33 0.22499319265761453 34-35 0.2345725971484955 36-37 0.20600983064455008 38-39 0.20422027156383601 40-41 0.20116749430850034 42-43 0.20941350183728066 44-45 0.2145014639295068 46-47 0.21934380026555653 48-49 0.22650203658841264 50-51 0.23222160776794962 52-53 0.2562227530857613 54-55 0.22730909264442092 56-57 0.1970971246347194 58-59 0.19667605190984552 60-61 0.19316711253589647 62-63 0.20218508672694557 64-65 0.19351800647329137 66-67 0.19316711253589647 68-69 0.1881142398374098 70-71 0.19850070038429907 72-73 0.19878141553421497 74-75 0.2040448245951386 76-77 0.20608000943202906 78-79 0.20446589732001247 80-81 0.2053782215572392 82-83 0.19667605190984552 84-85 0.18853531256228367 86-87 0.1910968383052665 88-89 0.19281621859850154 90-91 0.19800944887194616 92-93 0.1941847049543417 94-95 0.19123719588022445 96-97 0.19050031861169514 98-99 0.20078151097736593 100-101 0.1861492337879983 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1424932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.36423003680356 #Duplication Level Percentage of deduplicated Percentage of total 1 79.74835500718336 49.73444756724659 2 11.900821568832438 14.843711478912391 3 3.667450320587374 6.861531463249798 4 1.5981244556383174 3.98663204715478 5 0.8494812066152296 2.648862069064682 6 0.4999208635343564 1.8706307840192453 7 0.3297076123431153 1.439337296673591 8 0.2355929090901496 1.175405630203027 9 0.16794868408370223 0.9426591331717008 >10 0.9200326046759013 10.288361382756941 >50 0.055881880272745925 2.404680390088034 >100 0.025894813013780167 2.6519307952680213 >500 3.3774605552600723E-4 0.1522874616271234 >1k 3.3774605552600723E-4 0.6234728719450694 >5k 1.1258201850866909E-4 0.3760496286191207 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5356 0.37587758573742464 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3952 0.2773465681169347 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2788 0.1956584594914003 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2140 0.1501826052050203 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 3.508939373949073E-5 14-15 0.0 0.0 0.0 0.0 7.017878747898146E-5 16-17 0.0 0.0 0.0 0.0 7.017878747898146E-5 18-19 0.0 0.0 0.0 7.017878747898146E-5 7.017878747898146E-5 20-21 0.0 0.0 0.0 7.017878747898146E-5 7.017878747898146E-5 22-23 0.0 0.0 0.0 1.403575749579629E-4 7.017878747898146E-5 24-25 0.0 0.0 0.0 2.456257561764351E-4 7.017878747898146E-5 26-27 0.0 0.0 0.0 3.5089393739490726E-4 1.403575749579629E-4 28-29 0.0 0.0 0.0 9.123242372267589E-4 1.403575749579629E-4 30-31 0.0 0.0 0.0 0.0023860787742853696 1.403575749579629E-4 32-33 0.0 0.0 0.0 0.005158140879705137 1.403575749579629E-4 34-35 0.0 0.0 0.0 0.007368772685293052 1.403575749579629E-4 36-37 0.0 0.0 0.0 0.01112333781541856 1.403575749579629E-4 38-39 0.0 0.0 0.0 0.01410593628327527 1.403575749579629E-4 40-41 0.0 0.0 0.0 0.01642183627008166 1.403575749579629E-4 42-43 0.0 0.0 0.0 0.018948272619324993 1.403575749579629E-4 44-45 0.0 0.0 0.0 0.021650155937265776 1.403575749579629E-4 46-47 0.0 0.0 0.0 0.026808296816970917 1.403575749579629E-4 48-49 0.0 0.0 0.0 0.03312438769007925 1.403575749579629E-4 50-51 0.0 0.0 0.0 0.03915976341327165 1.403575749579629E-4 52-53 0.0 0.0 0.0 0.051827034553227805 2.1053636243694435E-4 54-55 0.0 0.0 0.0 0.07674050410826622 2.1053636243694435E-4 56-57 0.0 0.0 0.0 0.11719857508989903 2.1053636243694435E-4 58-59 0.0 0.0 0.0 0.15369154457896939 2.1053636243694435E-4 60-61 0.0 0.0 0.0 0.18790370347497284 2.1053636243694435E-4 62-63 0.0 0.0 0.0 0.23320411079265535 2.1053636243694435E-4 64-65 0.0 0.0 0.0 0.2914174150064705 2.1053636243694435E-4 66-67 0.0 0.0 0.0 0.39275558412611966 2.1053636243694435E-4 68-69 0.0 0.0 0.0 0.5151824788832029 2.1053636243694435E-4 70-71 0.0 0.0 0.0 0.6222753085761286 2.1053636243694435E-4 72-73 0.0 0.0 0.0 0.7455443487829594 2.1053636243694435E-4 74-75 0.0 0.0 0.0 0.8883581813026867 2.1053636243694435E-4 76-77 0.0 0.0 0.0 1.0454183076806471 2.1053636243694435E-4 78-79 0.0 0.0 0.0 1.1880216038379374 2.1053636243694435E-4 80-81 0.0 0.0 0.0 1.3416078802356886 2.1053636243694435E-4 82-83 0.0 0.0 0.0 1.499685599032094 2.807151499159258E-4 84-85 0.0 0.0 0.0 1.7461535006582771 3.5089393739490726E-4 86-87 0.0 0.0 0.0 2.0382025247520583 3.5089393739490726E-4 88-89 0.0 3.508939373949073E-5 0.0 2.4023953423742324 3.5089393739490726E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3635 0.0 39.265995 1 GTATCAA 6225 0.0 38.90256 1 TCAACGC 7770 0.0 30.837055 4 CAACGCA 7810 0.0 30.738909 5 ATCAACG 7835 0.0 30.640825 3 TATCAAC 7855 0.0 30.624409 2 AACGCAG 8060 0.0 29.702442 6 ACGCAGA 8915 0.0 26.747246 7 CGCAGAG 8985 0.0 26.43406 8 GTACATG 5830 0.0 25.624823 1 TACATGG 5920 0.0 24.573278 2 GCAGAGT 9460 0.0 24.45399 9 ACATGGG 5835 0.0 23.300928 3 GAGTACT 5175 0.0 22.717552 12-13 AGTACTT 5450 0.0 21.832724 12-13 GTACTTT 5375 0.0 21.738924 14-15 AGAGTAC 8360 0.0 21.42136 10-11 CAGAGTA 9290 0.0 19.353615 10-11 CATGGGA 2910 0.0 19.277575 4 TACTTTT 6260 0.0 18.855299 14-15 >>END_MODULE