FastQCFastQC Report
Thu 26 May 2016
SRR937362_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937362_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1424932
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59500.4175637854999396No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48840.34275319804734544No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT34170.2398009168156796No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47300.037.4743581
GTACCGT1201.506869E-731.6651696
GTATCAA71400.030.8155021
TCAACGC86150.025.4735954
ATCAACG86200.025.3486083
CAACGCA87250.025.152445
TATCAAC87700.024.9692132
AACGCAG90750.024.3376926
TACGACA1852.541965E-723.1086374
GTACATG57050.022.990051
GAGTACT56300.022.86705212-13
TACGTCA1302.9415326E-421.9235784
ACGCAGA100700.021.8424227
TACATGG58800.021.730942
CGCAGAG101050.021.7197558
GTACTTT59700.021.44161614-15
ACATGGG56900.020.5364993
CCTATAC3102.6011548E-1019.919813
GTATAGA4800.019.8004591
GCAGAGT109950.019.7455969
Produced by FastQC (version 0.11.5)