##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937362_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1424932 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.178190257500006 33.0 31.0 34.0 31.0 34.0 2 32.40528811199412 34.0 31.0 34.0 31.0 34.0 3 32.491253617716495 34.0 31.0 34.0 31.0 34.0 4 36.10660719248357 37.0 35.0 37.0 35.0 37.0 5 35.95923173877771 37.0 35.0 37.0 35.0 37.0 6 36.048046503271735 37.0 35.0 37.0 35.0 37.0 7 36.01116123436066 37.0 35.0 37.0 35.0 37.0 8 35.980435557626606 37.0 35.0 37.0 35.0 37.0 9 37.68806300932255 39.0 37.0 39.0 35.0 39.0 10-11 37.60703949381444 39.0 37.0 39.0 35.0 39.0 12-13 37.52499066622127 39.0 37.0 39.0 35.0 39.0 14-15 38.74515590919427 40.0 38.0 41.0 35.0 41.0 16-17 38.720658950742916 40.0 38.0 41.0 34.5 41.0 18-19 38.723829277467274 40.0 38.0 41.0 34.5 41.0 20-21 38.63521417162363 40.0 38.0 41.0 34.0 41.0 22-23 38.50972046385371 40.0 38.0 41.0 34.0 41.0 24-25 38.367781409919914 40.0 38.0 41.0 34.0 41.0 26-27 38.15704714330228 40.0 38.0 41.0 33.5 41.0 28-29 38.149709249283475 40.0 38.0 41.0 34.0 41.0 30-31 38.29014752984704 40.0 38.0 41.0 34.0 41.0 32-33 38.25691471593031 40.0 38.0 41.0 34.0 41.0 34-35 38.15809877243265 40.0 38.0 41.0 34.0 41.0 36-37 38.01295430238075 40.0 37.5 41.0 33.0 41.0 38-39 37.8820470029447 40.0 37.0 41.0 33.0 41.0 40-41 37.73304796299051 40.0 37.0 41.0 33.0 41.0 42-43 37.544198951248205 40.0 37.0 41.0 33.0 41.0 44-45 37.276940232937434 40.0 37.0 41.0 32.0 41.0 46-47 36.97004804439791 40.0 36.5 41.0 31.0 41.0 48-49 36.67935487447822 39.5 36.0 41.0 31.0 41.0 50-51 36.4841645074993 39.0 36.0 41.0 30.0 41.0 52-53 36.25685436217307 39.0 35.5 41.0 30.0 41.0 54-55 36.06793306627965 39.0 35.0 40.0 29.5 41.0 56-57 35.83593392526801 39.0 35.0 40.0 29.0 41.0 58-59 35.58477386991098 38.5 35.0 40.0 28.0 41.0 60-61 35.25236853407742 38.0 34.5 40.0 28.0 41.0 62-63 34.894807611872004 38.0 34.0 40.0 27.5 41.0 64-65 34.55367484202755 37.0 34.0 40.0 26.5 41.0 66-67 34.172768244379384 37.0 34.0 39.0 26.0 41.0 68-69 33.7256826992446 36.0 33.0 39.0 26.0 40.0 70-71 33.35372144074243 36.0 33.0 39.0 25.5 40.0 72-73 32.91720903172923 35.0 32.5 38.0 25.0 40.0 74-75 32.476435366740304 35.0 32.0 37.0 24.0 39.0 76-77 31.157019422681223 34.0 30.5 36.0 22.5 38.5 78-79 31.781228507746334 35.0 31.5 36.0 23.5 39.0 80-81 31.607989714596908 35.0 32.0 36.0 23.0 37.5 82-83 31.267678387459895 34.5 31.0 35.5 21.5 37.0 84-85 30.93439476410102 34.0 31.0 35.0 20.0 37.0 86-87 30.60657526113527 34.0 31.0 35.0 19.5 36.0 88-89 30.24491098522596 34.0 31.0 35.0 18.0 36.0 90-91 29.812968969747327 34.0 30.0 35.0 13.5 35.5 92-93 29.404934060011286 34.0 30.0 35.0 7.0 35.0 94-95 29.006447676099633 34.0 29.0 35.0 2.0 35.0 96-97 28.582374808061015 34.0 29.0 35.0 2.0 35.0 98-99 27.94951794190881 33.5 29.0 35.0 2.0 35.0 100-101 26.325984678567117 32.0 25.0 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.06094069733248375 1101 2 0.09236275188102638 1101 3 0.1392030683226011 1101 4 0.04442359823450204 1101 5 -0.02266557660546198 1101 6 -0.02910512777472718 1101 7 0.021119286556377403 1101 8 0.060534455336558324 1101 9 0.10996839204408815 1101 10-11 0.05677559259351028 1101 12-13 0.13794111490214078 1101 14-15 0.19561613501443986 1101 16-17 0.17286926009521864 1101 18-19 0.15970634787849747 1101 20-21 0.12452993916424049 1101 22-23 0.09543316778599831 1101 24-25 -0.07514416344874775 1101 26-27 -0.03178185452599536 1101 28-29 -0.006814016522270094 1101 30-31 0.09782727510627609 1101 32-33 0.09857421224515406 1101 34-35 0.12432253819930139 1101 36-37 0.12740058706408774 1101 38-39 0.08207353653972405 1101 40-41 0.16402370745135642 1101 42-43 0.06413344049780534 1101 44-45 0.026272598145638426 1101 46-47 -0.05101673104044835 1101 48-49 3.1930523744705397E-4 1101 50-51 -0.05878804615286981 1101 52-53 0.04524338927150495 1101 54-55 0.01365378038041598 1101 56-57 -0.12316825889180194 1101 58-59 -0.09748059315362667 1101 60-61 -0.005927907504407415 1101 62-63 -0.15208309964675237 1101 64-65 0.03482858223414809 1101 66-67 0.0041218842895887065 1101 68-69 0.05054015308490989 1101 70-71 -0.23528847253568586 1101 72-73 -0.16903600295605514 1101 74-75 -0.2811970880196313 1101 76-77 -0.17435502721090046 1101 78-79 -0.13378946599495478 1101 80-81 -0.12741823943936836 1101 82-83 -0.14336733060655504 1101 84-85 -0.039528353331967025 1101 86-87 -0.24672327854031906 1101 88-89 -0.10939168286696344 1101 90-91 -0.08445358084707166 1101 92-93 -0.1722729340179292 1101 94-95 -0.2503255239961355 1101 96-97 -0.19630750993546187 1101 98-99 -0.23240709661257952 1101 100-101 -0.09507204608764397 1102 1 0.07858706043124641 1102 2 0.04166667924736345 1102 3 0.008331325184187222 1102 4 0.03600011056622776 1102 5 -0.15876986574867402 1102 6 -0.08628918176296452 1102 7 -0.02027320657334286 1102 8 0.03444234137684532 1102 9 0.045748125581503984 1102 10-11 0.08033579017934755 1102 12-13 0.03031858037680024 1102 14-15 0.11510321192680806 1102 16-17 0.020779476813487463 1102 18-19 -0.01940468979499599 1102 20-21 -0.048041285659216726 1102 22-23 0.01608053968566736 1102 24-25 0.0836252733620455 1102 26-27 0.01841799241455533 1102 28-29 -0.058766915955253296 1102 30-31 -0.07919676218457283 1102 32-33 -0.02911302468390886 1102 34-35 0.037943437771424726 1102 36-37 0.026500667072440365 1102 38-39 -0.07606453004113689 1102 40-41 -0.01114013573228334 1102 42-43 0.11225115712910139 1102 44-45 -0.013606171812959644 1102 46-47 0.11404936281282119 1102 48-49 0.1627032061662348 1102 50-51 -0.12128291518268242 1102 52-53 -0.07191318358826493 1102 54-55 0.09445473352334943 1102 56-57 -0.16010393913669674 1102 58-59 -0.09027914604610743 1102 60-61 0.051182051795699124 1102 62-63 0.03738293159128858 1102 64-65 0.051500930653112675 1102 66-67 0.010194691886908913 1102 68-69 0.0620163071183768 1102 70-71 -0.07530116951276256 1102 72-73 -0.07566052029699222 1102 74-75 -0.1357359964274636 1102 76-77 -0.10132445010720303 1102 78-79 -0.09872250248571746 1102 80-81 -0.10322528017405475 1102 82-83 -0.12015725651186315 1102 84-85 -0.12344997993648477 1102 86-87 0.10162681016458919 1102 88-89 -0.03013819554654873 1102 90-91 -0.21749702885903588 1102 92-93 -0.16973440825872288 1102 94-95 -0.4053230889594346 1102 96-97 -0.17755859700541876 1102 98-99 -0.07772834752571711 1102 100-101 -0.024501551775195196 1103 1 0.007781926242209636 1103 2 0.05170305785948415 1103 3 -0.001992650380366001 1103 4 -0.02172176464826947 1103 5 -0.12208718334082391 1103 6 -0.019504227407828978 1103 7 -0.04127190243441703 1103 8 -0.0292620995593893 1103 9 -0.0740075199672674 1103 10-11 0.009692986967046124 1103 12-13 0.10980413189028582 1103 14-15 0.10582906819698223 1103 16-17 -0.06404961234843398 1103 18-19 -0.02810913073123089 1103 20-21 -0.052867629265506366 1103 22-23 -0.025219480905995795 1103 24-25 -0.05246265305706288 1103 26-27 -0.034970366429568855 1103 28-29 -0.1216758321471687 1103 30-31 -0.1082948471595202 1103 32-33 -0.047482507833066734 1103 34-35 -0.0104449210726969 1103 36-37 0.10632014678964907 1103 38-39 0.09327550702386844 1103 40-41 0.14396625454845235 1103 42-43 0.10859750897205345 1103 44-45 -0.06086988243139757 1103 46-47 -0.0703617155041556 1103 48-49 -0.20996135557444262 1103 50-51 -0.21531270446129014 1103 52-53 -0.06796301542298266 1103 54-55 -0.16770773473116662 1103 56-57 -0.1857693931829587 1103 58-59 -0.284529952553072 1103 60-61 -0.2877897376201872 1103 62-63 -0.23031972473378204 1103 64-65 -0.23611729983628038 1103 66-67 -0.12044399299195163 1103 68-69 -0.20476144015322717 1103 70-71 -0.09750138915720896 1103 72-73 -0.057601971323144596 1103 74-75 -0.15115503685576925 1103 76-77 -0.4615976328925697 1103 78-79 2.451685967166384E-4 1103 80-81 -0.027590370434193545 1103 82-83 -0.2276073217188106 1103 84-85 -0.213153116733789 1103 86-87 -0.07091694712829266 1103 88-89 0.02423410179712704 1103 90-91 0.2400635449620907 1103 92-93 0.10613730565438217 1103 94-95 -0.047642053884960234 1103 96-97 0.0064399615778718555 1103 98-99 0.024622534484027625 1103 100-101 0.29325327010688085 1104 1 0.04305441438313551 1104 2 0.17050435864901914 1104 3 0.11772489704592459 1104 4 0.12170525172921742 1104 5 0.01788736759139198 1104 6 0.050333013506893565 1104 7 0.03186142047335494 1104 8 0.11312369708063841 1104 9 0.10261221829762945 1104 10-11 0.09495037140702323 1104 12-13 0.07556317305922988 1104 14-15 0.17948667665291396 1104 16-17 0.17704421638629242 1104 18-19 0.12630415731772615 1104 20-21 0.022874757734079765 1104 22-23 0.011815328446537876 1104 24-25 0.10077809325289167 1104 26-27 0.0036760612778365953 1104 28-29 -0.07729868605161272 1104 30-31 0.09552057492209798 1104 32-33 0.0972851290793102 1104 34-35 0.0568383909416994 1104 36-37 0.12637331716339872 1104 38-39 0.04345133816181601 1104 40-41 0.14596214848037192 1104 42-43 0.17880866673088036 1104 44-45 0.24390241925232914 1104 46-47 0.3489247081399469 1104 48-49 0.22609852993148394 1104 50-51 0.37116166402356754 1104 52-53 0.4185322041888355 1104 54-55 0.4573275183761254 1104 56-57 0.3717416238307578 1104 58-59 0.332666747087778 1104 60-61 0.3092866269590928 1104 62-63 0.28800687876466213 1104 64-65 0.17603162265500316 1104 66-67 0.04696950490489371 1104 68-69 0.12490189434295473 1104 70-71 0.1746843435928227 1104 72-73 0.3033203435262024 1104 74-75 0.2079598461707448 1104 76-77 0.27695329398012447 1104 78-79 0.2127652678801617 1104 80-81 0.2619505839860423 1104 82-83 0.1508379594439333 1104 84-85 0.15528696501332107 1104 86-87 0.1890494905769593 1104 88-89 0.09782487971792264 1104 90-91 0.23156637657186252 1104 92-93 0.1720602821260222 1104 94-95 0.07602217079395146 1104 96-97 0.19452380984047934 1104 98-99 0.3440828511805698 1104 100-101 0.8142343824610414 1105 1 -0.026529218638522423 1105 2 -0.036985851532392644 1105 3 -0.02281094567764086 1105 4 -0.06618867192351274 1105 5 -0.19126644577603713 1105 6 -0.0963644211610486 1105 7 -0.03887101779085356 1105 8 -0.03897079131809278 1105 9 -0.0063790974007105206 1105 10-11 0.027319374310671662 1105 12-13 -0.05636062618955151 1105 14-15 0.013362445852941107 1105 16-17 -0.024483861079808378 1105 18-19 -0.02938758993179391 1105 20-21 -0.10578410371667957 1105 22-23 -0.1417169979340258 1105 24-25 -0.14017431624123589 1105 26-27 -0.15633918952799064 1105 28-29 -0.1773100076105223 1105 30-31 -0.24263041471535018 1105 32-33 -0.07897622577830532 1105 34-35 -0.1071044418455358 1105 36-37 -0.026563628413200036 1105 38-39 -0.09487779953497721 1105 40-41 -0.09967638744226548 1105 42-43 -0.17089863767254343 1105 44-45 0.06325429330330934 1105 46-47 0.01879970481007831 1105 48-49 0.01366079850549795 1105 50-51 0.18630258413332257 1105 52-53 0.15978996278656155 1105 54-55 0.16484803037697304 1105 56-57 0.09708826332568066 1105 58-59 0.01466613439165343 1105 60-61 0.10043649776012842 1105 62-63 0.21824476469662812 1105 64-65 0.1310460742919659 1105 66-67 -0.09168629306521581 1105 68-69 -0.1052237749609688 1105 70-71 -0.041788857611258834 1105 72-73 0.027663693109296617 1105 74-75 -0.03434748753553407 1105 76-77 0.08645940762193405 1105 78-79 0.06716669040073953 1105 80-81 0.12811876907355213 1105 82-83 0.10997612242110222 1105 84-85 0.08709721568595441 1105 86-87 0.09976634504831239 1105 88-89 0.28887685233306826 1105 90-91 0.09031760862386307 1105 92-93 0.22573412799298964 1105 94-95 0.2654122051582135 1105 96-97 0.08359051380169191 1105 98-99 0.09667315726224146 1105 100-101 0.1491543989771955 1106 1 -0.3216071143260386 1106 2 -0.1103150454813644 1106 3 -0.16589911456452455 1106 4 -0.17444392141550225 1106 5 -0.25748060247055093 1106 6 -0.20263841077495925 1106 7 -0.15075293244427712 1106 8 -0.155057944174267 1106 9 -0.1902270314716077 1106 10-11 -0.32768034345858155 1106 12-13 -0.38119269889218543 1106 14-15 -0.3413987869309807 1106 16-17 -0.22914913540810034 1106 18-19 -0.1699850724334908 1106 20-21 -0.3561566483091312 1106 22-23 -0.3476508994919456 1106 24-25 -0.11465642281157074 1106 26-27 -0.1722287808578571 1106 28-29 -0.14497765267712026 1106 30-31 -0.17314379245298994 1106 32-33 -0.25842841588894316 1106 34-35 -0.2293174131708966 1106 36-37 -0.25098505537187776 1106 38-39 -0.31063192010395824 1106 40-41 -0.28369224881051736 1106 42-43 -0.4896627492101331 1106 44-45 -0.30051151161878664 1106 46-47 -0.20169307008266912 1106 48-49 -0.5381962798417561 1106 50-51 -0.21959744973436557 1106 52-53 -0.13992075069278087 1106 54-55 -0.050872222501780584 1106 56-57 -0.19739977009044196 1106 58-59 -0.2340384264572961 1106 60-61 -0.07039918749613605 1106 62-63 -0.3042518485343564 1106 64-65 -0.2817672018426052 1106 66-67 -0.2961695285585506 1106 68-69 -0.2015773536469041 1106 70-71 -0.07843652074063101 1106 72-73 -0.32351603143349195 1106 74-75 -0.3268218045371114 1106 76-77 -0.32612385044986425 1106 78-79 -0.3168999725028492 1106 80-81 -0.22602296721003157 1106 82-83 -0.40150220225715216 1106 84-85 -0.47664601077600466 1106 86-87 -0.3484217335247308 1106 88-89 -0.21122757304512163 1106 90-91 -0.13603286552380212 1106 92-93 -0.2574985430388459 1106 94-95 -0.6243936200845077 1106 96-97 -0.4951913782732653 1106 98-99 -0.510388256149362 1106 100-101 -0.6470679424091195 1107 1 -0.10772562393453455 1107 2 -0.036167781970753765 1107 3 -0.043945266868561816 1107 4 -0.013739608330183728 1107 5 -0.1735347451517626 1107 6 -0.07871847206315863 1107 7 -0.08635163794589573 1107 8 -0.04162394354420229 1107 9 -0.18925794764425063 1107 10-11 -0.07192258219615155 1107 12-13 -0.07011088344412286 1107 14-15 -0.001269337092793421 1107 16-17 -0.009478201479069526 1107 18-19 -0.15743262663345092 1107 20-21 -0.1660622816682391 1107 22-23 -0.0755991410361716 1107 24-25 -0.10768220538166418 1107 26-27 0.039890315857206815 1107 28-29 -0.018959523971908254 1107 30-31 -0.11133453764873735 1107 32-33 -0.24293828930304073 1107 34-35 -0.028504592768221926 1107 36-37 -0.020362078025598862 1107 38-39 0.04920963272131473 1107 40-41 -0.0893433285138201 1107 42-43 0.00111806672479986 1107 44-45 0.04042037685960764 1107 46-47 0.1696289220701459 1107 48-49 0.1829617520624751 1107 50-51 0.01469834457785879 1107 52-53 0.1322860182163481 1107 54-55 0.06582988535399181 1107 56-57 0.10953166731827224 1107 58-59 0.12660789195494004 1107 60-61 -0.012788102637763643 1107 62-63 0.07350647835512802 1107 64-65 0.018268239346504345 1107 66-67 -0.00208623818423348 1107 68-69 0.06948724239514092 1107 70-71 0.0377311053440863 1107 72-73 0.15941062320465704 1107 74-75 0.12408874762945032 1107 76-77 0.13614041194381343 1107 78-79 0.3795474957790752 1107 80-81 0.3013600052675578 1107 82-83 0.21666731402431338 1107 84-85 0.24636695568732492 1107 86-87 0.1640456892206359 1107 88-89 0.20633586310346175 1107 90-91 0.23782138169102396 1107 92-93 0.4352499748750631 1107 94-95 0.27510984527620863 1107 96-97 0.22381655482522334 1107 98-99 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48-49 -0.1825079552213964 2316 50-51 -0.24585305092929133 2316 52-53 -0.4345451594555385 2316 54-55 -0.32159353193667783 2316 56-57 -0.11785657865404886 2316 58-59 -0.1034465668605904 2316 60-61 -0.02896123628877234 2316 62-63 0.11970570685524251 2316 64-65 0.27058841179941595 2316 66-67 0.0509940885686575 2316 68-69 0.03834360726920494 2316 70-71 0.22406534632434472 2316 72-73 0.029452601874957907 2316 74-75 -0.3092729496703015 2316 76-77 0.028856545367911934 2316 78-79 0.1023634703649634 2316 80-81 0.03467064742775605 2316 82-83 -0.06266102725092892 2316 84-85 -0.15998089999681397 2316 86-87 0.060216553754333546 2316 88-89 -0.08458766764307768 2316 90-91 -0.0877458976373191 2316 92-93 -0.25174798291935474 2316 94-95 0.14265579490180258 2316 96-97 0.21652134266275525 2316 98-99 -0.28625021781229165 2316 100-101 -0.6868846588641873 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 7.0 5 21.0 6 59.0 7 122.0 8 244.0 9 584.0 10 985.0 11 1684.0 12 2585.0 13 3614.0 14 4660.0 15 6592.0 16 8951.0 17 12536.0 18 11111.0 19 6861.0 20 5811.0 21 5904.0 22 6308.0 23 6756.0 24 7732.0 25 9633.0 26 11500.0 27 14651.0 28 18587.0 29 23587.0 30 29714.0 31 38257.0 32 48887.0 33 65380.0 34 90657.0 35 130251.0 36 204757.0 37 325732.0 38 288697.0 39 31507.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.089081299077705 20.476355639933946 15.650592021748654 21.783971039239695 2 15.529302450923973 21.075742561750317 40.003031723619095 23.39192326370662 3 19.195372130038486 25.218747280571986 30.308112948547723 25.27776764084181 4 13.088563586587702 17.457266351844407 37.58097760523141 31.87319245633648 5 13.367697305067283 38.19306993236699 34.49115211953272 13.948080643033014 6 31.524241156770987 37.35883536898603 17.24454219569776 13.87238127854522 7 28.14478164572064 33.24677949544259 20.844433278219594 17.76400558061718 8 26.461192534099876 35.76086437808962 19.548792503782636 18.22915058402787 9 26.265955147333347 16.960107570045448 20.464625680383346 36.30931160223786 10-11 24.640017909626565 27.425063090729946 27.369341133471632 20.56557786617186 12-13 25.5720397196729 24.520585007883366 28.225819752525815 21.681555519917914 14-15 22.58507774406077 25.965519758135823 26.483228673368274 24.96617382443513 16-17 21.652471837252584 29.417123062714573 27.167226225532165 21.763178874500678 18-19 21.68387684464943 27.75469285551872 29.730260812445785 20.831169487386063 20-21 22.321591486470936 26.692536907024333 30.087962092226157 20.897909514278577 22-23 22.11656282414237 26.645151917940314 29.985564041057916 21.252721216859403 24-25 21.957587853931653 26.873892044617183 29.93714743684216 21.23137266460901 26-27 21.71462077445217 27.092000583898983 29.92085782621145 21.27252081543739 28-29 21.66593503303931 27.23052635161856 29.903147147488568 21.200391467853564 30-31 21.861101139752595 27.024463573077544 29.82922835019224 21.285206936977623 32-33 21.762239097107475 26.98241985667381 29.72956945649668 21.52577158972203 34-35 22.084702989335632 27.043746648962895 29.687486841477345 21.18406352022412 36-37 22.09096032153181 26.834880895820074 29.689654712620765 21.38450407002735 38-39 21.897523099445166 27.205342593229403 29.66452292961178 21.23261137771365 40-41 22.12811633401325 27.14716461808866 29.49952331041878 21.22519573747931 42-43 21.85160268996576 27.14229214196051 29.407278966209788 21.59882620186394 44-45 22.000383176179636 27.286038912734085 29.32957502533454 21.38400288575174 46-47 22.201234865944482 27.55478857938484 28.76740784823416 21.47656870643652 48-49 22.21969188705145 27.605141859401012 28.597610271928765 21.577555981618772 50-51 22.265062473156615 27.792799937119806 28.514308051191218 21.427829538532364 52-53 22.500805303281776 27.769874849904802 28.228529281108038 21.50079056570538 54-55 22.18533937058049 27.900208571356387 28.304052403904183 21.610399654158936 56-57 22.27018552464258 28.05768977045922 28.192573399993826 21.479551304904373 58-59 22.37752398009168 27.650196640962516 28.293104513057465 21.67917486588834 60-61 22.21281436587851 27.814976433963164 28.533115966235584 21.43909323392274 62-63 22.163408499493308 27.825187447541357 28.628488938419515 21.382915114545817 64-65 22.128494552722515 27.70493609519612 28.525712104156547 21.640857247924814 66-67 22.16003991769432 27.857469689781688 28.231031375532307 21.75145901699169 68-69 22.09242265595832 27.756131520662038 28.47788526048962 21.673560562890017 70-71 22.17979524636965 27.74434148436557 28.334860891607462 21.74100237765732 72-73 22.243728121763002 27.704971184589862 28.260506466273476 21.79079422737366 74-75 22.16196983434999 28.04933849474922 27.980808908776 21.807882762124787 76-77 22.205796487130613 27.767605752414852 28.234470136118777 21.79212762433576 78-79 22.23699095816502 27.840310976243078 28.091375588449136 21.83132247714277 80-81 22.147407735948104 28.034109697866285 27.99445868294066 21.824023883244955 82-83 22.144752475386493 27.994342174241666 27.989675275048793 21.87123007532305 84-85 22.11503574559049 28.00482970754373 27.990934297871267 21.889200248994513 86-87 22.078316719675044 28.016494822209058 27.99137081629158 21.913817641824313 88-89 22.167057796442215 27.8687684745658 27.823643514216815 22.14053021477516 90-91 22.110050373811934 27.898529491752626 27.926111390868147 22.065308743567293 92-93 22.126020944539498 27.69863415551622 28.10371350811913 22.071631391825143 94-95 22.040455263830133 27.758482510042587 27.930771433303487 22.2702907928238 96-97 22.11845898611302 27.73935879045456 27.865224445798116 22.2769577776343 98-99 21.907798009004047 27.822536782895824 27.752918595144273 22.51674661295586 100-101 21.754586797785045 27.964121890679085 27.84304959016149 22.438241721374386 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 245.0 1 171.0 2 144.5 3 206.5 4 183.5 5 179.0 6 325.5 7 710.0 8 1194.0 9 1526.5 10 1512.0 11 1422.0 12 1524.0 13 1664.0 14 1807.0 15 1939.0 16 1994.5 17 2133.5 18 2390.5 19 2589.0 20 2840.5 21 3178.5 22 3685.0 23 4413.5 24 5312.5 25 6641.0 26 8649.5 27 10941.0 28 12859.0 29 15171.0 30 18112.5 31 21024.0 32 24519.5 33 27813.0 34 31048.5 35 34344.5 36 38483.5 37 41860.0 38 43951.5 39 45953.0 40 46736.5 41 48291.5 42 49493.0 43 50463.0 44 52635.0 45 53965.5 46 53590.0 47 54105.0 48 55944.5 49 55108.0 50 52590.5 51 49970.5 52 46939.5 53 45076.5 54 43875.5 55 40264.0 56 35302.5 57 31529.5 58 28362.0 59 24826.5 60 20257.0 61 16028.0 62 12690.0 63 9424.5 64 6643.0 65 4750.0 66 3370.5 67 2453.5 68 1735.0 69 1185.5 70 837.0 71 573.5 72 415.0 73 321.0 74 235.5 75 160.0 76 104.0 77 56.0 78 28.5 79 19.5 80 11.5 81 7.5 82 4.0 83 3.0 84 3.0 85 2.0 86 1.5 87 0.5 88 0.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.04631799973612776 2 0.0 3 0.0 4 7.017878747898146E-5 5 0.012912896896132587 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.006772252991721711 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0012632181746216663 24-25 7.017878747898145E-4 26-27 0.0019650060494114806 28-29 0.002701883317940786 30-31 0.0012281287808821755 32-33 3.508939373949073E-5 34-35 0.0 36-37 2.1053636243694435E-4 38-39 4.210727248738887E-4 40-41 3.508939373949073E-5 42-43 0.010912801452981616 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 2.1053636243694435E-4 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.1053636243694435E-4 84-85 7.017878747898146E-5 86-87 0.0 88-89 0.0 90-91 0.005930107541973933 92-93 0.001824648474453518 94-95 0.0 96-97 0.0 98-99 0.0011930393871426846 100-101 0.011474231752813467 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1424932.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.19656981167548 #Duplication Level Percentage of deduplicated Percentage of total 1 77.86139661636822 46.86988997051763 2 13.110533345392863 15.784182715884818 3 3.9910465700182836 7.207419404212609 4 1.7222321392006208 4.1468986879720555 5 0.9063824541119295 2.728055733751326 6 0.5224567049947376 1.88700609094762 7 0.3338495348768851 1.4067617782968265 8 0.26377017216531823 1.2702447666389793 9 0.19185964048452533 1.039436301822272 >10 1.004204314146684 10.78863600798957 >50 0.061529947606099526 2.529227421624091 >100 0.029923546904722816 3.0998756450170464 >500 3.4929159820009E-4 0.15923808867984243 >1k 3.4929159820009E-4 0.6661086244776507 >5k 1.1643053273336333E-4 0.4170187621677212 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5950 0.4175637854999396 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4884 0.34275319804734544 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3417 0.2398009168156796 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 7.017878747898146E-5 7.017878747898146E-5 20-21 0.0 0.0 0.0 7.017878747898146E-5 7.017878747898146E-5 22-23 0.0 0.0 0.0 1.403575749579629E-4 7.017878747898146E-5 24-25 0.0 0.0 0.0 2.456257561764351E-4 7.017878747898146E-5 26-27 0.0 0.0 0.0 3.5089393739490726E-4 1.0526818121847219E-4 28-29 0.0 0.0 0.0 0.001017592418445231 1.403575749579629E-4 30-31 0.0 0.0 0.0 0.002526436349243332 1.403575749579629E-4 32-33 0.0 0.0 0.0 0.00533358784840259 1.403575749579629E-4 34-35 0.0 0.0 0.0 0.007614398441469487 1.403575749579629E-4 36-37 0.0 0.0 0.0 0.011439142359073977 1.403575749579629E-4 38-39 0.0 0.0 0.0 0.01452700900814916 2.1053636243694435E-4 40-41 0.0 0.0 0.0 0.017018355963653004 2.1053636243694435E-4 42-43 0.0 0.0 0.0 0.01972023928159379 2.1053636243694435E-4 44-45 0.0 0.0 0.0 0.022351943812055592 2.1053636243694435E-4 46-47 0.0 0.0 0.0 0.02768553166045818 2.1053636243694435E-4 48-49 0.0 0.0 0.0 0.034141980108524476 2.1053636243694435E-4 50-51 0.0 0.0 0.0 0.0401071770442379 2.1053636243694435E-4 52-53 0.0 0.0 0.0 0.053090252727849466 2.1053636243694435E-4 54-55 0.0 0.0 0.0 0.07810899046410635 2.1053636243694435E-4 56-57 0.0 0.0 0.0 0.11923375992678949 2.1053636243694435E-4 58-59 0.0 0.0 0.0 0.1555863718409019 2.1053636243694435E-4 60-61 0.0 0.0 0.0 0.18958799437446838 2.1053636243694435E-4 62-63 0.0 0.0 0.0 0.23467786532971396 2.807151499159258E-4 64-65 0.0 0.0 0.0 0.29338242105588197 2.807151499159258E-4 66-67 0.0 0.0 0.0 0.3949662159317076 2.807151499159258E-4 68-69 0.0 0.0 0.0 0.518410703107236 2.807151499159258E-4 70-71 0.0 0.0 0.0 0.6256088009813802 2.807151499159258E-4 72-73 0.0 0.0 0.0 0.749088377550648 2.807151499159258E-4 74-75 0.0 0.0 0.0 0.8926039979451651 2.807151499159258E-4 76-77 0.0 0.0 0.0 1.0514887727975792 2.807151499159258E-4 78-79 0.0 0.0 0.0 1.195776359854365 2.807151499159258E-4 80-81 0.0 0.0 0.0 1.3515732680577037 3.5089393739490726E-4 82-83 0.0 0.0 0.0 1.5122826913845713 4.210727248738887E-4 84-85 0.0 0.0 0.0 1.7629262308657534 4.210727248738887E-4 86-87 0.0 0.0 0.0 2.060554468564114 4.210727248738887E-4 88-89 0.0 0.0 0.0 2.4318353437216653 4.210727248738887E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4730 0.0 37.474358 1 GTACCGT 120 1.506869E-7 31.665169 6 GTATCAA 7140 0.0 30.815502 1 TCAACGC 8615 0.0 25.473595 4 ATCAACG 8620 0.0 25.348608 3 CAACGCA 8725 0.0 25.15244 5 TATCAAC 8770 0.0 24.969213 2 AACGCAG 9075 0.0 24.337692 6 TACGACA 185 2.541965E-7 23.108637 4 GTACATG 5705 0.0 22.99005 1 GAGTACT 5630 0.0 22.867052 12-13 TACGTCA 130 2.9415326E-4 21.923578 4 ACGCAGA 10070 0.0 21.842422 7 TACATGG 5880 0.0 21.73094 2 CGCAGAG 10105 0.0 21.719755 8 GTACTTT 5970 0.0 21.441616 14-15 ACATGGG 5690 0.0 20.536499 3 CCTATAC 310 2.6011548E-10 19.91981 3 GTATAGA 480 0.0 19.800459 1 GCAGAGT 10995 0.0 19.745596 9 >>END_MODULE