FastQCFastQC Report
Thu 26 May 2016
SRR937361_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937361_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564703
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56230.3593653236428894No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42730.2730869692203568No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30470.19473344142626428No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN24270.15510930828406413No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA40350.036.7657471
GTATCAA65100.036.080981
ATCAACG80400.028.6687813
TCAACGC81200.028.444864
CAACGCA81500.028.2199125
TATCAAC82350.027.9899222
GTACATG65800.027.3870751
AACGCAG84700.027.311566
TACATGG66950.026.5487822
TATACCG1109.452934E-525.919495
ACATGGG67900.024.637493
GGACCGT1351.3429542E-524.6300456
ACGCAGA94950.024.0630687
CGCAGAG95700.023.8744898
ATACCGT1452.334733E-522.9314216
GCAGAGT103000.021.5828889
GAGTACT52100.021.37923412-13
GTGTAGC9350.021.3584631
CATGGGG32100.021.1716424
AGTACTT56050.020.67765212-13
Produced by FastQC (version 0.11.5)