##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937361_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1564703 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.404711309430606 31.0 31.0 34.0 30.0 34.0 2 31.51226398875697 33.0 31.0 34.0 30.0 34.0 3 31.550353645388295 33.0 31.0 34.0 28.0 34.0 4 35.194801185911956 37.0 35.0 37.0 33.0 37.0 5 35.17449126128089 37.0 35.0 37.0 33.0 37.0 6 35.23009734115676 37.0 35.0 37.0 32.0 37.0 7 35.157300778486395 37.0 35.0 37.0 32.0 37.0 8 35.14004127300836 37.0 35.0 37.0 32.0 37.0 9 36.72179704391185 39.0 37.0 39.0 32.0 39.0 10-11 36.61447316199943 39.0 37.0 39.0 32.0 39.0 12-13 36.485974654614964 39.0 37.0 39.0 32.0 39.0 14-15 37.59517109636781 40.0 37.0 41.0 32.0 41.0 16-17 37.52863322943715 40.0 37.0 41.0 32.0 41.0 18-19 37.47401327919739 40.0 37.0 41.0 32.0 41.0 20-21 37.39962472111321 40.0 37.0 41.0 32.0 41.0 22-23 37.261323075369575 40.0 37.0 41.0 31.5 41.0 24-25 37.11137033673483 39.0 36.5 41.0 31.0 41.0 26-27 36.93452239818036 39.0 36.0 41.0 31.0 41.0 28-29 36.74720026739899 39.0 36.0 40.5 30.0 41.0 30-31 36.74080001124814 39.0 36.0 41.0 30.0 41.0 32-33 36.59590829697393 39.0 36.0 41.0 30.0 41.0 34-35 36.394084692110894 39.0 35.0 40.0 30.0 41.0 36-37 36.40221754543833 39.0 35.0 41.0 30.0 41.0 38-39 36.37596336173702 39.0 35.0 41.0 30.0 41.0 40-41 36.21528366725187 39.0 35.0 41.0 29.5 41.0 42-43 35.98318946151443 39.0 35.0 41.0 29.0 41.0 44-45 35.77038390033125 39.0 35.0 41.0 27.5 41.0 46-47 35.460750378825885 39.0 35.0 40.0 26.5 41.0 48-49 35.17129640577157 39.0 35.0 40.0 25.5 41.0 50-51 34.25852829578521 38.0 33.5 39.5 24.0 40.5 52-53 34.407354622570544 38.0 33.5 39.5 24.0 40.5 54-55 34.76652182554773 38.0 34.0 40.0 24.5 41.0 56-57 34.540396164639546 38.0 34.0 40.0 23.5 41.0 58-59 34.294610223154166 38.0 34.0 40.0 23.0 41.0 60-61 34.01198502207767 38.0 33.5 40.0 22.5 41.0 62-63 33.68765126672602 37.5 33.0 40.0 22.0 41.0 64-65 33.327550979323235 37.0 33.0 40.0 20.5 41.0 66-67 32.93269233841822 36.0 32.0 39.0 19.5 41.0 68-69 32.42732710297098 36.0 31.5 39.0 17.5 40.5 70-71 32.00673961767824 35.0 31.0 38.5 14.5 40.0 72-73 31.584618294973552 35.0 31.0 38.0 12.0 40.0 74-75 31.11097153900772 35.0 30.5 37.0 8.0 39.0 76-77 30.658507077701007 35.0 30.0 37.0 7.0 39.0 78-79 30.17858820491812 34.0 30.0 36.0 4.0 39.0 80-81 29.718837057256234 34.0 29.0 36.0 2.0 37.5 82-83 29.256541656787263 34.0 29.0 35.0 2.0 37.0 84-85 28.770101738157337 34.0 29.0 35.0 2.0 36.5 86-87 28.312965783282834 34.0 27.5 35.0 2.0 36.0 88-89 27.896184132068512 34.0 27.0 35.0 2.0 36.0 90-91 27.435844374299787 33.0 26.0 35.0 2.0 35.0 92-93 26.963893467322553 33.0 25.5 35.0 2.0 35.0 94-95 26.387886391219293 33.0 25.0 35.0 2.0 35.0 96-97 25.71689611383119 32.0 23.5 34.5 2.0 35.0 98-99 24.959546636006962 32.0 19.0 34.0 2.0 35.0 100-101 23.104746076411946 30.5 2.0 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.07549383112913333 1101 2 0.20186148246000357 1101 3 0.12867422207488133 1101 4 0.12884190205084423 1101 5 0.27191348389024483 1101 6 0.043327873655968574 1101 7 0.025673432454354383 1101 8 -0.01120797640608373 1101 9 0.16107174332027085 1101 10-11 0.09369615551712229 1101 12-13 0.20225006837400628 1101 14-15 0.2824187641332898 1101 16-17 0.1020932758106099 1101 18-19 0.3426500903282914 1101 20-21 0.35445229345821616 1101 22-23 0.22716610313013064 1101 24-25 0.2281005025024001 1101 26-27 0.4324359862153315 1101 28-29 0.3590528456403348 1101 30-31 0.1915644426347427 1101 32-33 0.2660406902888326 1101 34-35 0.1761416610189812 1101 36-37 0.2834944545688174 1101 38-39 0.30519367110617424 1101 40-41 0.34936980158777686 1101 42-43 0.3724320518721882 1101 44-45 0.25214513873942934 1101 46-47 0.39152497872036207 1101 48-49 0.4522092596973195 1101 50-51 0.3397046125896068 1101 52-53 0.4356536122473642 1101 54-55 0.5962623021856572 1101 56-57 0.5763222680862086 1101 58-59 0.6194775219465001 1101 60-61 0.673700606946575 1101 62-63 0.700222587353224 1101 64-65 0.44597208560928436 1101 66-67 0.6307053141330172 1101 68-69 0.7160964873568005 1101 70-71 0.4846784414092937 1101 72-73 0.6427938469268106 1101 74-75 0.7527362280455954 1101 76-77 0.7623278345533748 1101 78-79 0.7378301716792421 1101 80-81 0.9952375484956626 1101 82-83 0.9113217409217391 1101 84-85 0.8262184021488927 1101 86-87 0.9806124727483763 1101 88-89 0.8728165478087568 1101 90-91 0.9513330458922091 1101 92-93 0.965680816813741 1101 94-95 1.0811781260068507 1101 96-97 1.1787954085731336 1101 98-99 1.2273111582731993 1101 100-101 1.4939923415099194 1102 1 0.1418752842337696 1102 2 -0.03536039818908776 1102 3 0.058310157669954066 1102 4 -0.17635828607712511 1102 5 0.0956367886365399 1102 6 0.05175619059379244 1102 7 0.11980573160935393 1102 8 0.16693489049600174 1102 9 0.12600400674146783 1102 10-11 0.011712364475791048 1102 12-13 0.10746976558256449 1102 14-15 0.34194642104672823 1102 16-17 0.3723268855554309 1102 18-19 0.24122356270815715 1102 20-21 0.336179444843431 1102 22-23 0.34866366482349775 1102 24-25 0.4222693893944012 1102 26-27 0.2643534332452191 1102 28-29 0.28314018851669687 1102 30-31 0.19027894530425726 1102 32-33 0.22020748832978398 1102 34-35 0.2220970410584968 1102 36-37 0.12732163695835652 1102 38-39 0.4018641213520979 1102 40-41 0.5443827682292195 1102 42-43 0.32513731104737076 1102 44-45 0.3247005261359064 1102 46-47 0.3364187232026481 1102 48-49 0.5249643359994991 1102 50-51 0.5079408734673976 1102 52-53 0.5122438322204559 1102 54-55 0.796496411152738 1102 56-57 0.6672542897787324 1102 58-59 0.3674579708261376 1102 60-61 0.35008108788154146 1102 62-63 0.533970078104467 1102 64-65 0.5353144785129658 1102 66-67 0.5695637241889315 1102 68-69 0.6913606810340198 1102 70-71 0.47947667678996453 1102 72-73 0.4277561556458558 1102 74-75 0.30619603539835083 1102 76-77 0.4046032239467401 1102 78-79 0.7662986497344839 1102 80-81 0.671746447789392 1102 82-83 0.6178181333840094 1102 84-85 0.7283580293612388 1102 86-87 0.455786018172347 1102 88-89 0.7313901647003398 1102 90-91 0.7553055413729304 1102 92-93 0.5083731881720439 1102 94-95 0.4663583189693945 1102 96-97 0.7240324997363743 1102 98-99 0.903791483661017 1102 100-101 1.0827281136753868 1103 1 0.05962496049793842 1103 2 0.05980194933533056 1103 3 0.13838209896585596 1103 4 -0.10151184077648878 1103 5 -0.20537198820156277 1103 6 -0.05675526405921261 1103 7 0.2073713980255576 1103 8 0.19911055515364495 1103 9 0.11082982887061377 1103 10-11 0.12546242442687827 1103 12-13 0.2064814520464111 1103 14-15 0.21231476048175324 1103 16-17 0.20265046151744315 1103 18-19 0.1447290222531663 1103 20-21 0.20908779163244873 1103 22-23 0.12525556583226916 1103 24-25 0.15941619759890813 1103 26-27 0.10877254724819352 1103 28-29 -0.017395054724815395 1103 30-31 -0.0767281369166426 1103 32-33 0.11255053640255142 1103 34-35 0.17315489784736116 1103 36-37 0.19259477407846504 1103 38-39 0.17007630021414855 1103 40-41 0.20997964118088674 1103 42-43 0.08135223966561966 1103 44-45 0.15399438234923224 1103 46-47 0.07358679405682977 1103 48-49 0.12597295296805555 1103 50-51 -0.05722467144794763 1103 52-53 0.07437345444871823 1103 54-55 0.1054700486645288 1103 56-57 0.2854928471159397 1103 58-59 0.1930359791713272 1103 60-61 0.18153446192831524 1103 62-63 0.1924009585060631 1103 64-65 0.16717839755503405 1103 66-67 0.14907516285497735 1103 68-69 0.15815993284454777 1103 70-71 0.2595868269074728 1103 72-73 0.3731976353983768 1103 74-75 0.448158274211476 1103 76-77 0.3564685818147204 1103 78-79 0.42952177966046534 1103 80-81 0.4350786087460534 1103 82-83 0.672204793869053 1103 84-85 0.4293963820654305 1103 86-87 0.6378539307556785 1103 88-89 0.5961208644493432 1103 90-91 0.7929847151671119 1103 92-93 0.6610167909921429 1103 94-95 0.9487309429082593 1103 96-97 1.0213764270916528 1103 98-99 0.968496604282592 1103 100-101 1.1868351623236926 1104 1 -0.08434567848069818 1104 2 -0.02696363302296234 1104 3 0.0062004119618706 1104 4 -0.16265334458777403 1104 5 -0.12184992038262266 1104 6 0.025344237179851348 1104 7 0.008258188702804148 1104 8 0.12675163548865953 1104 9 0.09560475907544941 1104 10-11 0.047531968640939226 1104 12-13 0.07947401768522866 1104 14-15 0.12806211767614428 1104 16-17 0.06913294214732701 1104 18-19 0.013069308962286641 1104 20-21 0.100057238603803 1104 22-23 0.11227601816766253 1104 24-25 0.11381940974482774 1104 26-27 0.2039711105657318 1104 28-29 0.06644916874518003 1104 30-31 0.16398644007761476 1104 32-33 -0.021830247185391727 1104 34-35 0.045544088223472556 1104 36-37 0.12182796834428444 1104 38-39 0.19030874163326672 1104 40-41 0.020152482236127867 1104 42-43 0.09756197020923452 1104 44-45 0.09634624407638626 1104 46-47 0.10383319450853179 1104 48-49 0.08132334037041744 1104 50-51 0.17871607324291716 1104 52-53 0.23181393250636972 1104 54-55 0.23664370530369894 1104 56-57 0.2152111569750943 1104 58-59 -0.049295806643783635 1104 60-61 0.08548502499491661 1104 62-63 0.19110574849320017 1104 64-65 0.0739468030884538 1104 66-67 0.046928608697072605 1104 68-69 -0.03288401458166135 1104 70-71 -0.03722078939166451 1104 72-73 0.043800069602713876 1104 74-75 -0.01012994060078043 1104 76-77 -0.013909096280578126 1104 78-79 -0.08019715757093948 1104 80-81 0.030325166040821472 1104 82-83 0.210983927467975 1104 84-85 0.05847900699093245 1104 86-87 0.18691067657581328 1104 88-89 0.15266812126417406 1104 90-91 0.3353525748866808 1104 92-93 0.3860304363386078 1104 94-95 0.28545151130751734 1104 96-97 0.4184476965794026 1104 98-99 0.4004273423860454 1104 100-101 0.82094382319762 1105 1 0.13116486120175708 1105 2 0.111717814856231 1105 3 0.055393494538833465 1105 4 0.018156416032915956 1105 5 -0.03097517604628308 1105 6 0.007698161497003753 1105 7 -0.0260724623766464 1105 8 -0.01714842483323764 1105 9 -3.0705569150768497E-4 1105 10-11 0.1786586113239892 1105 12-13 0.23381770649084643 1105 14-15 0.42728337608566136 1105 16-17 0.31433776764286847 1105 18-19 0.31938031124069255 1105 20-21 0.29130470437977607 1105 22-23 0.3522526258230343 1105 24-25 0.4035796718313023 1105 26-27 0.19488776645301442 1105 28-29 0.38854343995805607 1105 30-31 0.1897730901119985 1105 32-33 0.34110345251914964 1105 34-35 0.2873229886231812 1105 36-37 0.25541135151895844 1105 38-39 0.2495103281887836 1105 40-41 0.16768562405918175 1105 42-43 0.12363005838934527 1105 44-45 0.019510908764765134 1105 46-47 -0.06482365714955307 1105 48-49 -0.1651394720131094 1105 50-51 -0.3030962667471684 1105 52-53 -0.3613989256628898 1105 54-55 -0.1629563579638571 1105 56-57 -0.02156285107550815 1105 58-59 0.012491712999256777 1105 60-61 -0.041084657040812544 1105 62-63 0.10638390159299149 1105 64-65 0.04730879465875404 1105 66-67 0.18558732380225962 1105 68-69 0.13732522939671554 1105 70-71 0.08767339114626083 1105 72-73 -0.00915048437257937 1105 74-75 0.15747624066459665 1105 76-77 0.13765479049867935 1105 78-79 0.11209489517933946 1105 80-81 0.16548575547924216 1105 82-83 0.12207188601363939 1105 84-85 0.01208029462647886 1105 86-87 0.0552719061179161 1105 88-89 0.3253818125415755 1105 90-91 0.33084620266599885 1105 92-93 0.2929896748739296 1105 94-95 0.4279471024300818 1105 96-97 0.4758100096103419 1105 98-99 0.445456164982037 1105 100-101 0.7783237141059196 1106 1 -0.05522697918087616 1106 2 -0.009270363511191704 1106 3 -0.16438771343762326 1106 4 -0.07866169014230451 1106 5 -0.07899455041727776 1106 6 -0.13949013945900646 1106 7 0.019473778636367456 1106 8 -0.07343925539328211 1106 9 -0.0625991452135537 1106 10-11 -0.035980456947648065 1106 12-13 -0.24814871360899105 1106 14-15 -0.2245398965195733 1106 16-17 -0.390389817303479 1106 18-19 -0.17107600563736014 1106 20-21 -0.20301239486702372 1106 22-23 -0.22258618810658248 1106 24-25 -0.18345994481579453 1106 26-27 -0.10053504184539008 1106 28-29 -0.03278573312716304 1106 30-31 -0.008403172149009208 1106 32-33 0.007964843696285584 1106 34-35 0.04253877450454269 1106 36-37 -0.29328780720913983 1106 38-39 -0.3977222466090282 1106 40-41 -0.06710286584448966 1106 42-43 -0.1993674288547922 1106 44-45 -0.22192727099805154 1106 46-47 -0.12090345480603304 1106 48-49 -0.08479726450213576 1106 50-51 -0.16301813805947774 1106 52-53 -0.11457311894008626 1106 54-55 0.006556640112556522 1106 56-57 -0.12136763271933404 1106 58-59 -0.2119211934630414 1106 60-61 -0.02384921219550762 1106 62-63 0.11271471642807285 1106 64-65 0.04746194425162997 1106 66-67 -0.006200240312978167 1106 68-69 0.03272646927101164 1106 70-71 -0.011774787958479749 1106 72-73 0.1724327344287495 1106 74-75 0.13566939244876508 1106 76-77 -0.05397049362051831 1106 78-79 -0.14756309025404946 1106 80-81 -0.07612452376983114 1106 82-83 -0.22883707146314336 1106 84-85 -0.1430945250308291 1106 86-87 -0.2226193221831636 1106 88-89 -0.019124665048796174 1106 90-91 -0.05830415646893172 1106 92-93 0.016870089332215343 1106 94-95 -0.06833131078164456 1106 96-97 0.26790728389213214 1106 98-99 0.24058008178029056 1106 100-101 0.8093045502510137 1107 1 -0.06561277463770665 1107 2 -0.26308616233243853 1107 3 -0.11045080899646109 1107 4 -0.09878284937843773 1107 5 0.013096991223086718 1107 6 -0.0462391472239716 1107 7 -0.24380829759784461 1107 8 -0.1606805404130469 1107 9 -0.08253532851666279 1107 10-11 -0.08682638673990084 1107 12-13 -0.03319495089022695 1107 14-15 0.03730861623370885 1107 16-17 -0.013216350090459628 1107 18-19 -0.16568673853078053 1107 20-21 -0.07415353552513437 1107 22-23 -0.03897545789397583 1107 24-25 0.07963581508007422 1107 26-27 0.10925715511913126 1107 28-29 -0.10019320829950829 1107 30-31 0.027317456554698083 1107 32-33 -0.07295927863982854 1107 34-35 0.008593345085593285 1107 36-37 0.033764386833290416 1107 38-39 -0.028698946343496345 1107 40-41 0.061087969000148234 1107 42-43 0.09278999370664565 1107 44-45 0.19046852924402202 1107 46-47 0.10093027327666704 1107 48-49 0.09715705984646661 1107 50-51 0.08297572556499944 1107 52-53 0.08498441326649697 1107 54-55 0.18595749234225423 1107 56-57 0.1553677565276672 1107 58-59 0.2602442849343163 1107 60-61 0.18126647818694863 1107 62-63 0.2979128216311224 1107 64-65 0.4019906041277963 1107 66-67 0.4262558982742064 1107 68-69 0.4048043856913779 1107 70-71 0.4992703193610666 1107 72-73 0.37144589452810095 1107 74-75 0.3017027176056253 1107 76-77 0.5117743763132552 1107 78-79 0.5724743271602293 1107 80-81 0.5217627628879065 1107 82-83 0.6900064134298205 1107 84-85 0.5173323873589375 1107 86-87 0.46688282447594176 1107 88-89 0.5380422810923768 1107 90-91 0.8155745944877815 1107 92-93 0.6802886188271628 1107 94-95 0.73421224233795 1107 96-97 0.8608171429753213 1107 98-99 1.2235359904879637 1107 100-101 1.2116878579155852 1108 1 0.1315487706346019 1108 2 -0.08096268415378205 1108 3 -0.1292048734684066 1108 4 -0.11974785882653549 1108 5 0.11721550707277117 1108 6 -0.006466093283556518 1108 7 -0.044659393232556965 1108 8 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-1.6638279053967935 2316 92-93 -1.4524369521043354 2316 94-95 -1.4953345381582537 2316 96-97 -1.8487549049909013 2316 98-99 -1.940645377061383 2316 100-101 -1.9897100375811796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7149.0 3 1491.0 4 1427.0 5 1637.0 6 1811.0 7 2168.0 8 2695.0 9 3276.0 10 4201.0 11 5157.0 12 6547.0 13 7673.0 14 8854.0 15 10281.0 16 12767.0 17 15675.0 18 13722.0 19 10796.0 20 10674.0 21 11409.0 22 12588.0 23 13687.0 24 15413.0 25 17651.0 26 20345.0 27 24041.0 28 28370.0 29 33659.0 30 40898.0 31 50757.0 32 63225.0 33 80843.0 34 107318.0 35 147957.0 36 211387.0 37 289222.0 38 235445.0 39 32481.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.38961658667799 19.70376717490423 15.233978908130496 22.672637330287284 2 14.75981877663329 20.12029415780947 41.15574452044212 23.964142545115116 3 18.72353465745793 24.923853539792656 29.995326392802262 26.357285409947156 4 12.20934817186419 16.703733075695613 38.22291518278153 32.86400356965867 5 13.245975069371042 37.73810340307508 34.667124760189964 14.348796767363915 6 31.19572444060705 36.85428177237924 17.837996062119615 14.11199772489409 7 27.982840535743225 32.777151803095336 21.179075805142585 18.060931856018847 8 26.072646794118302 35.35940657799073 19.91916034372868 18.64878628416228 9 25.918476760489856 16.88785350234019 20.72195793105171 36.47171180611824 10-11 24.317269198505763 27.110873951157078 27.771384352546384 20.80047249779077 12-13 25.278261150864612 24.295735288135734 28.405073799940233 22.02092976105942 14-15 22.388746527882297 25.686337677176475 26.591506032854547 25.333409762086685 16-17 21.559105001537358 29.06973839294865 27.27612227118992 22.095034334324076 18-19 21.531212331987053 27.614520366506163 29.716083256796416 21.13818404471037 20-21 22.230626966526025 26.541203974284045 29.931386756122752 21.29678230306718 22-23 21.970969212993207 26.501753657830484 29.747653890199754 21.77962323897655 24-25 21.618959482783463 26.755964865652427 29.84603906022345 21.77903659134066 26-27 21.534809553960006 26.908920063296794 29.72957306049481 21.826697322248386 28-29 21.484860402612345 27.04835498756088 29.816667136389608 21.650117473437167 30-31 21.669063549971014 26.875862797915513 29.674963406158017 21.78011024595546 32-33 21.498351095042008 26.872570523677865 29.62756784596896 22.001510535311162 34-35 21.935907433591193 26.897773109176416 29.57237995654144 21.59393950069095 36-37 21.782552680871177 26.707450755342542 29.625574396909265 21.884422166877012 38-39 21.59347802419484 27.052302608406077 29.584435379029006 21.76978398837008 40-41 21.87533721916548 26.917109968110474 29.502566307894828 21.70498650482922 42-43 21.53867386331324 26.894627363481032 29.40057048879644 22.166128284409286 44-45 21.721312394211477 27.046216273777894 29.298116664024455 21.93435466798617 46-47 21.8821253080695 27.324643766620383 28.748808712538494 22.04442221277163 48-49 21.926468200348644 27.315311379759716 28.67197010482719 22.08625031506445 50-51 21.945893971093742 27.54162913841104 28.564308803199907 21.948168087295315 52-53 22.029155792799045 27.49878995355536 28.39382391013335 22.078230343512246 54-55 21.816156870382315 27.56807729488406 28.492751042131786 22.123014792601843 56-57 21.963848200094517 27.72345608491901 28.260123387692737 22.05257232729373 58-59 22.012302777008568 27.353366296537583 28.47879987898908 22.155531047464773 60-61 21.87075678266435 27.54348112149485 28.6312815624933 21.954480533347507 62-63 21.815177418229066 27.51188007036152 28.780571050216793 21.892371461192624 64-65 21.757463234905686 27.41912607722775 28.684537513634933 22.138873174231634 66-67 21.727594622824753 27.56260210606671 28.408365348715233 22.301437922393305 68-69 21.71445402984262 27.47692007229336 28.635118629307627 22.17350726855639 70-71 21.807689056580575 27.3868529376356 28.582872906949042 22.22258509883478 72-73 21.790980538083218 27.393959229055238 28.488583968944965 22.326476263916582 74-75 21.713593714327615 27.75360794405706 28.300190935816044 22.232607405799286 76-77 21.82218594294853 27.529862884200124 28.346626897523258 22.301324275328092 78-79 21.7730177359415 27.512180750651265 28.320761635158696 22.394039878248535 80-81 21.78790124919354 27.72889474467816 28.150799736809667 22.332404269318634 82-83 21.76552001521724 27.627903908512113 28.28782998705638 22.31874608921427 84-85 21.798041739888784 27.594352081503782 28.20146201985153 22.406144158755904 86-87 21.744868668983724 27.618207520135503 28.2645607170816 22.372363093799176 88-89 21.90992688440063 27.5114214104173 28.051662849122742 22.526988856059326 90-91 21.777990534802854 27.432004918316245 28.246665086999123 22.543339459881782 92-93 21.8554215864373 27.174874049708936 28.428725088132385 22.54097927572138 94-95 21.67545543855694 27.237774598421222 28.38432827696095 22.702441686060883 96-97 21.662919290417072 27.257567383930066 28.264556011160796 22.81495731449207 98-99 21.55147292868703 27.2400703721565 28.189210670078964 23.019246029077507 100-101 21.57687560730025 27.004422986724634 28.576144045616154 22.84255736035896 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2223.0 1 1262.0 2 301.5 3 307.0 4 267.5 5 242.5 6 399.0 7 812.5 8 1343.0 9 1626.0 10 1522.5 11 1424.5 12 1507.5 13 1630.5 14 1789.0 15 1879.0 16 1935.0 17 2056.0 18 2292.5 19 2561.0 20 2810.0 21 3247.5 22 3860.5 23 4592.0 24 5615.0 25 7157.0 26 9376.0 27 11868.0 28 14076.0 29 16724.0 30 19812.0 31 23069.5 32 26875.0 33 30503.0 34 34125.0 35 37541.5 36 41704.5 37 45861.5 38 48174.0 39 50070.0 40 51378.0 41 52744.5 42 53995.5 43 55538.5 44 57555.0 45 58508.0 46 58406.0 47 59085.5 48 60900.5 49 60189.0 50 57504.0 51 54467.5 52 51739.0 53 49930.5 54 47748.5 55 43962.5 56 39212.5 57 35045.5 58 31584.5 59 27605.0 60 22818.5 61 18377.5 62 14309.5 63 10670.5 64 7865.0 65 5836.5 66 4160.0 67 2943.0 68 2126.5 69 1512.0 70 1135.0 71 787.5 72 508.5 73 355.0 74 260.5 75 197.5 76 130.0 77 72.0 78 54.5 79 40.0 80 23.0 81 16.0 82 15.0 83 10.5 84 5.5 85 4.0 86 4.5 87 3.5 88 4.0 89 6.5 90 4.5 91 2.0 92 2.0 93 2.0 94 1.5 95 2.0 96 2.0 97 1.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3530382443185704 2 0.31060207592111727 3 0.3118802737644141 4 0.31251937268606245 5 0.2948802424485669 6 0.22093649721384828 7 0.22892523373445312 8 0.22930869308744214 9 0.22394026214559568 10-11 0.2324722327496017 12-13 0.23164140415145879 14-15 0.21320340026190274 16-17 0.22898914362661796 18-19 0.21505678713468307 20-21 0.21828423668900743 22-23 0.21697408389962825 24-25 0.20885752759469367 26-27 0.22282183903271102 28-29 0.22362071268477146 30-31 0.2331432866173325 32-33 0.23435757456846443 34-35 0.2202015334539526 36-37 0.21675039927705128 38-39 0.20534248352562756 40-41 0.20569398793253416 42-43 0.20674850115325402 44-45 0.2243556764446671 46-47 0.21518460691901273 48-49 0.22461131601332648 50-51 0.23371847564681603 52-53 0.2436564638784485 54-55 0.23774479885320088 56-57 0.19946277344646238 58-59 0.18281424653752182 60-61 0.19297591939173123 62-63 0.19518081067141815 64-65 0.19156990176410477 66-67 0.20540639341779238 68-69 0.19240073036224767 70-71 0.1985680349561546 72-73 0.2138424991835511 74-75 0.2034571417067648 76-77 0.2146413728356116 78-79 0.21253234639417193 80-81 0.19783307119625895 82-83 0.21192520241860596 84-85 0.18914132586184088 86-87 0.19783307119625895 88-89 0.20908121221727063 90-91 0.20406428568233076 92-93 0.2092090320016003 94-95 0.20713196050624305 96-97 0.2023387185938801 98-99 0.21026354522232013 100-101 0.1901638841364783 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1564703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.22013321810608 #Duplication Level Percentage of deduplicated Percentage of total 1 79.07861373093486 48.41203267310984 2 12.08563616341567 14.797685118997354 3 3.904754109015963 7.17148700213713 4 1.6843941446677089 4.124753357134199 5 0.8948452800235887 2.739127362631877 6 0.5275549141104909 1.9378189273026456 7 0.3412456268831476 1.4623771916517692 8 0.25132282419821056 1.2308813422532083 9 0.17047639279661272 0.9392928729795729 >10 0.9708827680425273 10.593606934301492 >50 0.06222096868181607 2.6106602396755116 >100 0.02732312871192921 2.844128053665252 >500 2.0855671926959935E-4 0.08745991043992976 >1k 4.171134385391987E-4 0.6897203448124096 >5k 1.0427835963479968E-4 0.35896866890782847 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5623 0.3593653236428894 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 4273 0.2730869692203568 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3047 0.19473344142626428 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 2427 0.15510930828406413 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 6.390989216483896E-5 6.390989216483896E-5 14-15 0.0 0.0 0.0 6.390989216483896E-5 6.390989216483896E-5 16-17 0.0 0.0 0.0 9.586483824725843E-5 6.390989216483896E-5 18-19 0.0 0.0 0.0 2.2368462257693633E-4 6.390989216483896E-5 20-21 0.0 0.0 0.0 2.556395686593558E-4 1.9172967649451684E-4 22-23 0.0 0.0 0.0 2.875945147417753E-4 1.9172967649451684E-4 24-25 0.0 0.0 0.0 3.834593529890337E-4 1.9172967649451684E-4 26-27 0.0 0.0 0.0 5.432340834011311E-4 1.9172967649451684E-4 28-29 0.0 0.0 0.0 0.0010545132207198428 1.9172967649451684E-4 30-31 0.0 0.0 0.0 0.0025883506326759773 1.9172967649451684E-4 32-33 0.0 0.0 0.0 0.005368430941846472 1.9172967649451684E-4 34-35 0.0 0.0 0.0 0.008691745334418098 1.9172967649451684E-4 36-37 0.0 0.0 0.0 0.012270699295649078 1.9172967649451684E-4 38-39 0.0 0.0 0.0 0.015402284011726186 1.9172967649451684E-4 40-41 0.0 0.0 0.0 0.017958679698319747 1.9172967649451684E-4 42-43 0.0 0.0 0.0 0.020994399576149594 1.9172967649451684E-4 44-45 0.0 0.0 0.0 0.023135380963671702 1.9172967649451684E-4 46-47 0.0 0.0 0.0 0.02754516352304559 1.9172967649451684E-4 48-49 0.0 0.0 0.0 0.03425570220035368 1.9172967649451684E-4 50-51 0.0 0.0 0.0 0.03968804303436499 1.9172967649451684E-4 52-53 0.0 0.0 0.0 0.05352453468805262 1.9172967649451684E-4 54-55 0.0 0.0 0.0 0.07947195090697723 1.9172967649451684E-4 56-57 0.0 0.0 0.0 0.12270699295649079 1.9172967649451684E-4 58-59 0.0 0.0 0.0 0.16469579210878998 1.9172967649451684E-4 60-61 0.0 0.0 0.0 0.2013161603192427 1.9172967649451684E-4 62-63 0.0 0.0 0.0 0.2507185069626632 1.9172967649451684E-4 64-65 0.0 0.0 0.0 0.31136899462709533 1.9172967649451684E-4 66-67 0.0 0.0 0.0 0.41704400132165653 1.9172967649451684E-4 68-69 0.0 0.0 0.0 0.5415724262048452 1.9172967649451684E-4 70-71 0.0 0.0 0.0 0.6498038285859999 2.2368462257693633E-4 72-73 0.0 0.0 0.0 0.7701461555323917 2.556395686593558E-4 74-75 0.0 0.0 0.0 0.9013851190928885 2.556395686593558E-4 76-77 0.0 0.0 0.0 1.04959215902315 2.556395686593558E-4 78-79 0.0 0.0 0.0 1.1831638336476635 2.556395686593558E-4 80-81 0.0 0.0 0.0 1.332968620882046 2.556395686593558E-4 82-83 0.0 0.0 0.0 1.4895478566859013 3.834593529890337E-4 84-85 0.0 6.390989216483896E-5 0.0 1.7245445301760143 3.834593529890337E-4 86-87 0.0 6.390989216483896E-5 0.0 2.0082405414957343 3.834593529890337E-4 88-89 0.0 6.390989216483896E-5 0.0 2.3548558416517382 3.834593529890337E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4035 0.0 36.765747 1 GTATCAA 6510 0.0 36.08098 1 ATCAACG 8040 0.0 28.668781 3 TCAACGC 8120 0.0 28.44486 4 CAACGCA 8150 0.0 28.219912 5 TATCAAC 8235 0.0 27.989922 2 GTACATG 6580 0.0 27.387075 1 AACGCAG 8470 0.0 27.31156 6 TACATGG 6695 0.0 26.548782 2 TATACCG 110 9.452934E-5 25.91949 5 ACATGGG 6790 0.0 24.63749 3 GGACCGT 135 1.3429542E-5 24.630045 6 ACGCAGA 9495 0.0 24.063068 7 CGCAGAG 9570 0.0 23.874489 8 ATACCGT 145 2.334733E-5 22.931421 6 GCAGAGT 10300 0.0 21.582888 9 GAGTACT 5210 0.0 21.379234 12-13 GTGTAGC 935 0.0 21.358463 1 CATGGGG 3210 0.0 21.171642 4 AGTACTT 5605 0.0 20.677652 12-13 >>END_MODULE