FastQCFastQC Report
Thu 26 May 2016
SRR937361_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937361_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1564703
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63360.40493307675641954No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53690.34313221103302033No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37250.2380643483140251No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA45650.036.5475431
GTATCAA70250.033.492751
GTCTACG1056.8751826E-527.1615051
ATCAACG87700.026.4348033
TCAACGC88900.026.077984
TATCAAC90050.025.9032122
CAACGCA90600.025.5886575
GTACATG63600.025.0368421
AACGCAG93500.024.840256
TACATGG66200.023.6098792
ACATGGG64300.023.2731653
GGACCGT1302.943766E-421.9213146
ACGCAGA106450.021.5527047
CGCAGAG107000.021.3975288
CATGGGG31500.020.6616924
GAGTACT61400.020.57849512-13
TGAACCG2551.1907105E-820.4931325
CGTAGAC1404.8481286E-420.3600623
AGTACTT65150.019.90437512-13
GTACTTT63850.019.6010114-15
Produced by FastQC (version 0.11.5)