##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937361_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1564703 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.09614987636631 31.0 31.0 34.0 31.0 34.0 2 32.31602419117238 34.0 31.0 34.0 31.0 34.0 3 32.403429916092705 34.0 31.0 34.0 31.0 34.0 4 36.03009069452797 37.0 35.0 37.0 35.0 37.0 5 35.91180179241684 37.0 35.0 37.0 35.0 37.0 6 35.99485908827426 37.0 35.0 37.0 35.0 37.0 7 35.9441772655897 37.0 35.0 37.0 35.0 37.0 8 35.92079966613473 37.0 35.0 37.0 35.0 37.0 9 37.60746927691709 39.0 37.0 39.0 35.0 39.0 10-11 37.52740136626568 39.0 37.0 39.0 35.0 39.0 12-13 37.45043564177995 39.0 37.0 39.0 34.5 39.0 14-15 38.647015439990845 40.0 38.0 41.0 34.0 41.0 16-17 38.62074240287134 40.0 38.0 41.0 34.0 41.0 18-19 38.62316299003709 40.0 38.0 41.0 34.0 41.0 20-21 38.51080684321561 40.0 38.0 41.0 34.0 41.0 22-23 38.388916618680994 40.0 38.0 41.0 34.0 41.0 24-25 38.2576792528678 40.0 38.0 41.0 34.0 41.0 26-27 38.21027217305776 40.0 38.0 41.0 34.0 41.0 28-29 38.11821093204269 40.0 38.0 41.0 33.0 41.0 30-31 38.206747222955414 40.0 38.0 41.0 34.0 41.0 32-33 38.13981535153955 40.0 38.0 41.0 34.0 41.0 34-35 38.04743967385504 40.0 37.0 41.0 33.0 41.0 36-37 37.897663006973204 40.0 37.0 41.0 33.0 41.0 38-39 37.77701519074227 40.0 37.0 41.0 33.0 41.0 40-41 37.62420472127937 40.0 37.0 41.0 33.0 41.0 42-43 37.42307198235065 40.0 37.0 41.0 32.5 41.0 44-45 37.157127582678626 40.0 36.5 41.0 31.5 41.0 46-47 36.83968011820774 40.0 36.0 41.0 31.0 41.0 48-49 36.556710442812474 39.0 36.0 41.0 30.5 41.0 50-51 36.35263305560224 39.0 36.0 41.0 30.0 41.0 52-53 36.13363590406614 39.0 35.0 40.5 29.5 41.0 54-55 35.90827268817149 39.0 35.0 40.0 29.0 41.0 56-57 35.66089922496474 38.5 35.0 40.0 28.0 41.0 58-59 35.42842379672052 38.0 35.0 40.0 28.0 41.0 60-61 35.10321032170323 38.0 34.0 40.0 27.5 41.0 62-63 34.74044211585202 38.0 34.0 40.0 26.5 41.0 64-65 34.384771742624636 37.0 34.0 40.0 26.0 41.0 66-67 33.95434820537827 36.5 33.0 39.0 26.0 41.0 68-69 33.55666123219551 36.0 33.0 39.0 25.5 40.0 70-71 33.156756585754614 35.5 32.5 38.5 25.0 40.0 72-73 32.70581669492549 35.0 32.0 38.0 24.0 40.0 74-75 32.25644387465225 35.0 32.0 37.0 22.5 39.0 76-77 30.923260836081994 34.0 30.0 36.0 21.5 38.5 78-79 31.57431633990604 34.5 31.0 36.0 22.0 39.0 80-81 31.43783420879234 35.0 31.0 36.0 22.0 37.5 82-83 31.09617032753181 34.0 31.0 35.5 20.5 37.0 84-85 30.72353922757226 34.0 31.0 35.0 19.5 37.0 86-87 30.347131372535234 34.0 31.0 35.0 18.0 36.0 88-89 29.941445437249115 34.0 30.0 35.0 15.0 36.0 90-91 29.489561277763258 34.0 29.5 35.0 8.5 35.5 92-93 29.113031993931116 34.0 29.0 35.0 4.5 35.0 94-95 28.679761910087727 34.0 29.0 35.0 2.0 35.0 96-97 28.178782810539765 33.5 29.0 35.0 2.0 35.0 98-99 27.656423615216433 33.0 28.5 35.0 2.0 35.0 100-101 25.696206244891204 31.5 23.5 34.5 2.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.21411968381953983 1101 2 0.1439863322081223 1101 3 0.1090863643449893 1101 4 0.10452078785093022 1101 5 0.016932674342243104 1101 6 0.04226848628947266 1101 7 0.03516750399445101 1101 8 0.04257940702481733 1101 9 0.059611291136960176 1101 10-11 0.008425445786890862 1101 12-13 -0.041588093417082916 1101 14-15 0.03145649517024651 1101 16-17 0.12358030633458839 1101 18-19 0.052283060575994966 1101 20-21 0.16736486299116393 1101 22-23 0.06436629293856555 1101 24-25 0.10212106052284753 1101 26-27 0.01838640967553573 1101 28-29 7.301582323293587E-4 1101 30-31 0.14807590877553167 1101 32-33 0.1752298695142258 1101 34-35 0.09834553712727967 1101 36-37 0.11049869697684045 1101 38-39 0.05536689574864084 1101 40-41 0.12940302346387256 1101 42-43 0.21140022907943035 1101 44-45 0.19247871080763446 1101 46-47 0.23656840544115454 1101 48-49 0.16275824189937538 1101 50-51 0.12363360679068336 1101 52-53 0.13390742752022078 1101 54-55 0.26933228722688796 1101 56-57 0.17580466502347747 1101 58-59 0.0639452443130537 1101 60-61 0.12725493264088783 1101 62-63 0.12215185772976866 1101 64-65 0.14893604233727586 1101 66-67 0.27225600439364683 1101 68-69 0.15591034805199655 1101 70-71 -0.17236321147094458 1101 72-73 -0.16846945392207857 1101 74-75 -0.007772463205938607 1101 76-77 -0.06048942953752956 1101 78-79 -0.026452987775691383 1101 80-81 -0.013131349852866236 1101 82-83 -0.003100712504743086 1101 84-85 0.009983105007240312 1101 86-87 0.13330312886314033 1101 88-89 0.1308061545737793 1101 90-91 0.07338477759583739 1101 92-93 -0.00361252671154233 1101 94-95 -0.0872822891108882 1101 96-97 -0.07921508774996155 1101 98-99 0.03936560866800676 1101 100-101 0.43531962623465503 1102 1 0.21463401412601257 1102 2 0.1723224506761909 1102 3 0.2559109166214384 1102 4 0.1813964184474699 1102 5 0.025795840147097238 1102 6 0.11989623463941257 1102 7 0.11779535744381775 1102 8 0.16357840332531737 1102 9 0.26297867668368724 1102 10-11 0.20947013406199488 1102 12-13 0.1514417784667188 1102 14-15 0.1019253305635317 1102 16-17 0.16947919543367362 1102 18-19 0.22879754327663449 1102 20-21 0.3327509588628601 1102 22-23 0.32308888826373305 1102 24-25 0.3459372731600041 1102 26-27 0.33888480428183954 1102 28-29 0.24618756406324138 1102 30-31 0.2667233054627971 1102 32-33 0.1894311007539784 1102 34-35 0.29076977357996014 1102 36-37 0.24685731723160131 1102 38-39 0.15618253296644724 1102 40-41 0.3509647614930529 1102 42-43 0.2762784319843803 1102 44-45 0.2656729056409475 1102 46-47 0.3090686156400011 1102 48-49 0.1686208191474563 1102 50-51 0.1041169721793409 1102 52-53 0.18966562790278374 1102 54-55 0.057364155999749755 1102 56-57 0.14585428508906517 1102 58-59 0.18741342020745577 1102 60-61 0.3129196339483187 1102 62-63 0.17597064530280448 1102 64-65 0.11317826235241313 1102 66-67 0.12837620660494053 1102 68-69 0.3163094762522789 1102 70-71 0.3037453903914127 1102 72-73 0.2464193902995575 1102 74-75 0.1913883178929794 1102 76-77 0.2646688107828119 1102 78-79 0.2388889006507604 1102 80-81 0.1417218131143052 1102 82-83 0.12343078316515843 1102 84-85 0.1534648387273414 1102 86-87 0.12101831993389567 1102 88-89 0.34076294557306497 1102 90-91 0.21747477423265948 1102 92-93 0.14001207653813097 1102 94-95 0.16870443683185243 1102 96-97 0.20259916478293505 1102 98-99 0.31916326600184064 1102 100-101 0.7439345603373191 1103 1 0.1786531345237634 1103 2 0.09957253460770232 1103 3 0.09280017498661408 1103 4 0.0823895280700242 1103 5 -0.08356452179755536 1103 6 -0.006568889819028811 1103 7 -0.04549955390852034 1103 8 -0.013387272683061724 1103 9 0.056043216019588726 1103 10-11 -0.037343985616345776 1103 12-13 0.10985475347910523 1103 14-15 0.04156098786717166 1103 16-17 0.09161793022607867 1103 18-19 0.040864176903589566 1103 20-21 -0.027236891051614975 1103 22-23 -0.0485441791532466 1103 24-25 -0.010631286894991376 1103 26-27 0.023593601902980765 1103 28-29 -0.033655763781240466 1103 30-31 0.024690084571041382 1103 32-33 0.027633527573691197 1103 34-35 0.1282945133922766 1103 36-37 0.10439932295493293 1103 38-39 0.17694015474707925 1103 40-41 0.26833282914984835 1103 42-43 0.4490526482239261 1103 44-45 0.24029153683788707 1103 46-47 0.32681749255643666 1103 48-49 0.4229157145128326 1103 50-51 0.4104148730921935 1103 52-53 0.49170460409821004 1103 54-55 0.5054477997464559 1103 56-57 0.33417565485132883 1103 58-59 0.3285996522421115 1103 60-61 0.3550450995683718 1103 62-63 0.3038328697276782 1103 64-65 0.26804549069408523 1103 66-67 0.3720362600013658 1103 68-69 0.4460477698047427 1103 70-71 0.2797819045958292 1103 72-73 0.38869881029284414 1103 74-75 0.45906765155670826 1103 76-77 0.48588429894447316 1103 78-79 0.3951132615722486 1103 80-81 0.38729134837353385 1103 82-83 0.3484996070049071 1103 84-85 0.4914958853932596 1103 86-87 0.17414233074107344 1103 88-89 0.25230344852265674 1103 90-91 0.2735367076949551 1103 92-93 0.41286846572455715 1103 94-95 0.3537994792772139 1103 96-97 0.41049767959485095 1103 98-99 0.557752736993514 1103 100-101 0.929779231085984 1104 1 0.08261216711575514 1104 2 0.059830698289125905 1104 3 0.019114049323952997 1104 4 -0.04753393251531435 1104 5 0.01427888608851191 1104 6 -0.07211893844037576 1104 7 -0.12174755415946947 1104 8 0.003270087961880108 1104 9 0.04828983605158754 1104 10-11 -0.00968063356640414 1104 12-13 0.01951283560213568 1104 14-15 0.03470380916018456 1104 16-17 -0.03830851037744765 1104 18-19 -0.02119297670849818 1104 20-21 -0.22221163931849475 1104 22-23 -0.19357444986437855 1104 24-25 -0.23907697214232826 1104 26-27 -0.26255103876956554 1104 28-29 -0.15016522141792876 1104 30-31 -0.11244000106527352 1104 32-33 0.012949880031420946 1104 34-35 0.03799086522263195 1104 36-37 0.005558706875383734 1104 38-39 -0.1752710187527171 1104 40-41 -0.08703151728511216 1104 42-43 -3.866378264518744E-4 1104 44-45 0.07412478129348443 1104 46-47 0.20844520986249648 1104 48-49 0.05594294246854048 1104 50-51 -0.20168745132199462 1104 52-53 0.03200982069847669 1104 54-55 0.38348835936250225 1104 56-57 0.17800670350405312 1104 58-59 0.15559438231520772 1104 60-61 0.1758531550280793 1104 62-63 0.03009028056239771 1104 64-65 -0.02980026983792783 1104 66-67 0.04061212499085798 1104 68-69 0.12570240138038713 1104 70-71 -0.01963118866306246 1104 72-73 0.08769328941703236 1104 74-75 0.020814726433592057 1104 76-77 0.022671839331561472 1104 78-79 0.08020893584057731 1104 80-81 0.033752027817680386 1104 82-83 0.1697261197562483 1104 84-85 0.20242721950035403 1104 86-87 0.23419863740063462 1104 88-89 0.12350533691313004 1104 90-91 0.16373730997231561 1104 92-93 -0.02553173503456918 1104 94-95 0.20536649831796439 1104 96-97 0.24295274405735157 1104 98-99 0.013200210251770983 1104 100-101 0.49353734771449 1105 1 -0.02295083084406002 1105 2 0.11185513185883877 1105 3 0.0696410014288773 1105 4 -0.016508818883465892 1105 5 0.05767254985605774 1105 6 -0.03386416231657563 1105 7 0.022626372437365205 1105 8 0.06120408223193863 1105 9 0.0709204726589121 1105 10-11 0.0562072701644567 1105 12-13 0.06393747869758926 1105 14-15 0.038709259903065174 1105 16-17 0.0890035087923593 1105 18-19 0.08711044114806299 1105 20-21 0.20130954570579007 1105 22-23 0.056088400684608075 1105 24-25 0.02448424817859518 1105 26-27 0.11929632890328179 1105 28-29 0.05087654797312524 1105 30-31 0.13607820628580214 1105 32-33 0.09708739858584892 1105 34-35 -0.015571072301291622 1105 36-37 -0.02065506316628074 1105 38-39 0.04261461554290946 1105 40-41 -0.00819617813133533 1105 42-43 -0.15327911116506243 1105 44-45 0.03497862428493903 1105 46-47 -0.029326478804797773 1105 48-49 0.15691696178947723 1105 50-51 0.22188528266869412 1105 52-53 0.22294110880789475 1105 54-55 0.15914613698090108 1105 56-57 0.11140832054069705 1105 58-59 0.0801489480167632 1105 60-61 0.11064723451722358 1105 62-63 0.023474723148375176 1105 64-65 0.06556227628399114 1105 66-67 0.010075605910060403 1105 68-69 0.0867758877938698 1105 70-71 0.17877566011340207 1105 72-73 0.2234372466614829 1105 74-75 0.1993721814387044 1105 76-77 0.14428033357664916 1105 78-79 0.051099298553193506 1105 80-81 -0.013556475776660193 1105 82-83 -0.012387093300432639 1105 84-85 -0.03351784274902414 1105 86-87 0.13911945050393015 1105 88-89 0.21380487803127934 1105 90-91 0.06618409286855709 1105 92-93 -0.19660322352537918 1105 94-95 0.10710824504028338 1105 96-97 0.2407308555337515 1105 98-99 0.429575299742055 1105 100-101 0.8822055081271891 1106 1 -0.24572715560526603 1106 2 -0.06383036330704783 1106 3 -0.08024128527887342 1106 4 0.011583192151576327 1106 5 -0.17632142269967233 1106 6 -0.2530479318362566 1106 7 -0.12122812545764816 1106 8 -0.10066969700731931 1106 9 -0.11993734077835683 1106 10-11 -0.31590937435380084 1106 12-13 -0.3231409680037771 1106 14-15 -0.3258691170282262 1106 16-17 -0.07617926637473715 1106 18-19 -0.12244454300121532 1106 20-21 -0.1579392598479714 1106 22-23 -0.12781083963139395 1106 24-25 -0.13214471738807276 1106 26-27 -0.1808324270086601 1106 28-29 -0.07868973031260396 1106 30-31 -0.11482096615512916 1106 32-33 -0.17337323197701693 1106 34-35 -0.33209958276042784 1106 36-37 -0.04204191283609049 1106 38-39 -0.09189620674120391 1106 40-41 -0.1932825431317795 1106 42-43 -0.5624183346535645 1106 44-45 -0.37739620736248725 1106 46-47 -0.04427036472505819 1106 48-49 -0.17572309256571117 1106 50-51 -0.215149562050712 1106 52-53 -0.08459274862207167 1106 54-55 -0.21140145636540808 1106 56-57 -0.1494238691952816 1106 58-59 -0.25807753490714447 1106 60-61 -0.09097773606737292 1106 62-63 -0.12051535262019542 1106 64-65 -0.15177577362037908 1106 66-67 -0.2579759763888205 1106 68-69 -0.10206858560754739 1106 70-71 -0.061039030601939714 1106 72-73 -0.07407539033332 1106 74-75 -0.3484635512382468 1106 76-77 -0.2585439240785057 1106 78-79 -0.2748566115912361 1106 80-81 -0.184239888176986 1106 82-83 -0.2204986123893491 1106 84-85 -0.5012789059551892 1106 86-87 -0.21907229554269136 1106 88-89 -0.12469534471610544 1106 90-91 -0.37730439644938585 1106 92-93 -0.3634339503295898 1106 94-95 -1.0698338003285173 1106 96-97 -0.6852815252929538 1106 98-99 -0.45433799794939844 1106 100-101 0.09938420665540093 1107 1 -0.01564122222072939 1107 2 -0.07502777418014972 1107 3 -0.10764788248573609 1107 4 -0.0677503717314707 1107 5 -0.13820264134256632 1107 6 -0.0031189733177825474 1107 7 -0.11413805156109902 1107 8 -0.10836127778751603 1107 9 -0.12942674018295008 1107 10-11 -0.08314179259242849 1107 12-13 -0.08548995459033648 1107 14-15 -0.16082266528214717 1107 16-17 -0.19148696311607694 1107 18-19 -0.1619538273186052 1107 20-21 -0.05089382144830523 1107 22-23 -0.15638392456082073 1107 24-25 -0.17773072918632238 1107 26-27 -0.17581528992674578 1107 28-29 -0.17771837209330243 1107 30-31 -0.08211222818693642 1107 32-33 -0.14411553503462216 1107 34-35 -0.003014999164044241 1107 36-37 -0.23313478549892608 1107 38-39 -0.21200004123867444 1107 40-41 -0.19033465674989003 1107 42-43 0.055835740513686005 1107 44-45 0.09643137362144927 1107 46-47 -0.03181029204828434 1107 48-49 -0.013847369584595981 1107 50-51 -0.05507902992321334 1107 52-53 -0.15947398871144713 1107 54-55 -0.10220131642709873 1107 56-57 -0.05294836443215445 1107 58-59 -0.05072353520471751 1107 60-61 0.021278360431089993 1107 62-63 -0.03157359591180153 1107 64-65 -0.014182665716411691 1107 66-67 -0.025151092300362166 1107 68-69 -0.039635186996470395 1107 70-71 -0.03885549024300161 1107 72-73 -0.060016884322273256 1107 74-75 -0.3056689335017424 1107 76-77 -0.2944391405740703 1107 78-79 -0.3186634028887916 1107 80-81 -0.19537446755109755 1107 82-83 0.10888622199687248 1107 84-85 -0.010120051840882383 1107 86-87 -0.04028467874312369 1107 88-89 -0.1849815815684046 1107 90-91 -0.059080111790191125 1107 92-93 -0.17199005902573816 1107 94-95 -0.10949052923020375 1107 96-97 -0.15028689361622938 1107 98-99 -0.1408520460672662 1107 100-101 0.2035254893964762 1108 1 0.03203623150657364 1108 2 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94-95 -0.5099227414769523 2316 96-97 -0.4797377668549565 2316 98-99 -0.7824338232686259 2316 100-101 -1.3074802356581934 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 0.0 4 12.0 5 34.0 6 49.0 7 128.0 8 368.0 9 675.0 10 1164.0 11 1884.0 12 2996.0 13 4136.0 14 5394.0 15 7560.0 16 9999.0 17 14132.0 18 12280.0 19 7721.0 20 6738.0 21 6728.0 22 7258.0 23 8066.0 24 9301.0 25 11421.0 26 13922.0 27 17334.0 28 21996.0 29 27825.0 30 35062.0 31 44461.0 32 57201.0 33 75492.0 34 102987.0 35 147360.0 36 226265.0 37 349397.0 38 296676.0 39 30677.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.93754244256905 20.478195869498773 15.714013497216534 21.870248190715643 2 15.532212822497304 21.010952238220288 39.96963001924327 23.48720492003914 3 19.20076845254339 25.099779319142357 30.28453323090708 25.414918997407177 4 13.12536628356947 17.526968376746257 37.45042988988965 31.897235449794625 5 13.292477199810019 38.165283878106635 34.498012274738855 14.044226647344491 6 31.536655838200605 37.32497477157007 17.252475389898276 13.885894000331053 7 28.21832641721784 33.19173031559344 20.813981950568255 17.77596131662047 8 26.40622533477599 35.78838923425084 19.52012618369109 18.28525924728207 9 26.238270138166797 16.824854301423336 20.549331087113657 36.38754447329621 10-11 24.612050977086387 27.4369960305566 27.368804175616713 20.5821488167403 12-13 25.596619052184195 24.523791263221806 28.183715207873966 21.695874476720036 14-15 22.5782784336708 25.969529041613647 26.390663276033855 25.061529248681698 16-17 21.651808681903212 29.403822961929514 27.205546356081634 21.73882200008564 18-19 21.686895212701707 27.757600004601514 29.723148738131133 20.832356044565646 20-21 22.38265025375423 26.719000347030715 30.033431264591425 20.86491813462363 22-23 22.146598414573624 26.61761327087579 29.883341071822876 21.35244724272771 24-25 21.949780756554453 26.803334059777633 29.996855625267223 21.25002955840069 26-27 21.751059477071134 27.068321012445303 29.8384597047205 21.34215980576307 28-29 21.671792393983086 27.194124459306074 29.90612633064392 21.227956816066918 30-31 21.86204027217945 27.032094908746263 29.801182716464403 21.30468210260989 32-33 21.798417974529354 26.979976391685835 29.727494610798345 21.49411102298647 34-35 22.141582140508454 26.99080911840778 29.623321486569655 21.244287254514116 36-37 22.07137712396538 26.88142733796765 29.647191831293224 21.400003706773745 38-39 21.902399369081543 27.145726697015345 29.661923061437218 21.289950872465894 40-41 22.094448595036887 27.103130753887477 29.524133333929825 21.27828731714581 42-43 21.85085044775547 27.055903916342277 29.419771552027203 21.67347408387505 44-45 22.041083835079245 27.22423999953985 29.288114102165075 21.44656206321583 46-47 22.220990181523266 27.53503380513746 28.697618653508044 21.546357359831227 48-49 22.245787219683226 27.557753771802062 28.537811968149867 21.658647040364848 50-51 22.294678287189328 27.766291749935927 28.44648473224631 21.492545230628433 52-53 22.466531987220577 27.791440292502795 28.21835837216392 21.52366934811271 54-55 22.22712553117109 27.81169972831905 28.27702765317124 21.68414708733862 56-57 22.25454287490981 28.069480278365926 28.12712700109861 21.548849845625657 58-59 22.385270559332984 27.664930660962494 28.261593414213433 21.688205365491086 60-61 22.242527815182818 27.83112833553716 28.425650107400575 21.50069374187945 62-63 22.162832179653265 27.808024909519567 28.58395491029288 21.445188000534287 64-65 22.097644089645126 27.652500186936436 28.542541300170065 21.707314423248373 66-67 22.106176060249133 27.83457946971406 28.257151676707977 21.802092793328832 68-69 22.141741915238867 27.736861244594024 28.400693294510205 21.720703545656907 70-71 22.17082091617387 27.740695838123912 28.32221194693178 21.766271298770437 72-73 22.239442398616475 27.632673825431297 28.22463957993279 21.903244196019433 74-75 22.095311378581112 28.02720388469889 27.941340944575426 21.93614379214458 76-77 22.19264614434816 27.812179052510285 28.16016841534783 21.83500638779372 78-79 22.17983221096911 27.80562828856339 28.057848677991927 21.956690822475576 80-81 22.13151633249249 28.060884397869756 27.898745001447562 21.908854268190193 82-83 22.16913076975434 28.00146098246917 27.946626207379623 21.88278204039687 84-85 22.10951525458609 27.966794424664005 27.9134926889064 22.010197631843507 86-87 22.09690912588523 27.977194394079895 27.92453264293607 22.001363837098797 88-89 22.188971325548682 27.903410423575593 27.74539321519803 22.162225035677697 90-91 22.1824838442774 27.815582678214696 27.892410445636205 22.1095230318717 92-93 22.159014288572017 27.64960435923836 28.001434161811034 22.18994719037859 94-95 21.979474699032338 27.702669452285832 27.99157411981699 22.32628172886484 96-97 22.123527595971886 27.6881938617105 27.84646670965672 22.341811832660895 98-99 21.932642765203852 27.776503129669422 27.752409084924796 22.53844502020193 100-101 21.777977344235627 27.800978266253967 27.961220899295462 22.45982349021494 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 307.0 1 207.0 2 140.5 3 206.0 4 210.0 5 209.0 6 354.5 7 794.0 8 1351.5 9 1651.0 10 1575.5 11 1452.5 12 1594.5 13 1747.5 14 1890.5 15 2069.5 16 2176.5 17 2313.0 18 2537.0 19 2721.5 20 2993.0 21 3435.5 22 4032.5 23 4818.5 24 5757.5 25 7207.0 26 9346.5 27 11769.0 28 14066.0 29 16675.5 30 19635.5 31 22851.0 32 26738.0 33 30287.0 34 33948.0 35 37588.5 36 41985.0 37 45912.5 38 48564.5 39 50600.5 40 51481.0 41 53029.0 42 54223.5 43 55568.0 44 57882.0 45 59174.5 46 58973.5 47 59839.5 48 61626.0 49 60247.0 50 57400.5 51 54643.5 52 51542.0 53 49762.0 54 48113.5 55 44088.0 56 39177.0 57 34907.0 58 31290.5 59 27688.5 60 22690.0 61 17955.0 62 14002.5 63 10264.0 64 7294.5 65 5202.5 66 3772.0 67 2849.5 68 1979.0 69 1332.0 70 971.0 71 682.5 72 457.0 73 341.0 74 272.0 75 171.0 76 97.0 77 56.0 78 34.0 79 21.0 80 13.5 81 8.5 82 4.5 83 2.0 84 2.5 85 1.5 86 0.5 87 0.5 88 0.5 89 1.0 90 0.5 91 0.5 92 0.5 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.05272566103599214 2 0.0 3 0.0 4 0.0 5 0.02249628204202331 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0033872242847364643 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 8.627835442253259E-4 24-25 2.556395686593558E-4 26-27 0.0010225582746374233 28-29 0.003003764931747431 30-31 0.0 32-33 0.0 34-35 0.0 36-37 0.0 38-39 0.0 40-41 0.0 42-43 0.014891004874407476 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 0.0 72-73 6.390989216483896E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.5977473041209737E-4 84-85 0.0016616571962858127 86-87 0.0 88-89 0.0 90-91 0.010513177261116008 92-93 0.0016616571962858127 94-95 0.0 96-97 0.0 98-99 6.390989216483896E-5 100-101 0.01281393337905021 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1564703.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.96716274584346 #Duplication Level Percentage of deduplicated Percentage of total 1 77.06490330408108 45.44298695124438 2 13.344793090486512 15.738091719526508 3 4.178908318644041 7.392551007763267 4 1.8463273275617804 4.354907360257349 5 0.9822927390803596 2.896150790470595 6 0.5689481649909913 2.012955542338367 7 0.3949006253451977 1.6300318610215938 8 0.2694394394688084 1.2710463421844849 9 0.20413239772301417 1.0833397486408998 >10 1.0481901661466986 11.059642297800169 >50 0.06517877703915562 2.655598568324954 >100 0.03100987474777477 3.182846113438737 >500 5.420970470283424E-4 0.21225374064651184 >1k 2.1683881881133697E-4 0.3192756568640603 >5k 2.1683881881133697E-4 0.7483222994781389 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6336 0.40493307675641954 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5369 0.34313221103302033 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3725 0.2380643483140251 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 3.195494608241948E-5 16-17 0.0 0.0 0.0 0.0 6.390989216483896E-5 18-19 0.0 0.0 0.0 9.586483824725843E-5 6.390989216483896E-5 20-21 0.0 0.0 0.0 1.278197843296779E-4 6.390989216483896E-5 22-23 0.0 0.0 0.0 2.2368462257693633E-4 6.390989216483896E-5 24-25 0.0 0.0 0.0 3.1954946082419475E-4 6.390989216483896E-5 26-27 0.0 0.0 0.0 4.7932419123629215E-4 6.390989216483896E-5 28-29 0.0 0.0 0.0 9.906033285550038E-4 6.390989216483896E-5 30-31 0.0 0.0 0.0 0.002620305578758397 6.390989216483896E-5 32-33 0.0 0.0 0.0 0.00549625072617615 6.390989216483896E-5 34-35 0.0 0.0 0.0 0.008883475010912615 6.390989216483896E-5 36-37 0.0 0.0 0.0 0.012558293810390853 6.390989216483896E-5 38-39 0.0 0.0 0.0 0.0157537884186328 6.390989216483896E-5 40-41 0.0 0.0 0.0 0.018438003889556038 6.390989216483896E-5 42-43 0.0 0.0 0.0 0.021569588605633145 6.390989216483896E-5 44-45 0.0 0.0 0.0 0.02383838977748493 6.390989216483896E-5 46-47 0.0 0.0 0.0 0.028471856959435753 6.390989216483896E-5 48-49 0.0 0.0 0.0 0.035118485744579 6.390989216483896E-5 50-51 0.0 0.0 0.0 0.04077451120116725 6.390989216483896E-5 52-53 0.0 0.0 0.0 0.05483468747743182 6.390989216483896E-5 54-55 0.0 0.0 0.0 0.08081405864243885 6.390989216483896E-5 56-57 0.0 0.0 0.0 0.12427278531452934 6.390989216483896E-5 58-59 0.0 0.0 0.0 0.16712436801105385 6.390989216483896E-5 60-61 0.0 0.0 0.0 0.20374473622150657 6.390989216483896E-5 62-63 0.0 0.0 0.0 0.25343467737966885 6.390989216483896E-5 64-65 0.0 0.0 0.0 0.3145325342892549 6.390989216483896E-5 66-67 0.0 0.0 0.0 0.4210064146358766 6.390989216483896E-5 68-69 0.0 0.0 0.0 0.545822434033807 6.390989216483896E-5 70-71 0.0 0.0 0.0 0.6540218814688794 6.390989216483896E-5 72-73 0.0 0.0 0.0 0.7755465414203206 6.390989216483896E-5 74-75 0.0 0.0 0.0 0.9079678379858669 6.390989216483896E-5 76-77 0.0 0.0 0.0 1.0571015713525187 6.390989216483896E-5 78-79 0.0 0.0 0.0 1.1921751284429059 6.390989216483896E-5 80-81 0.0 0.0 0.0 1.344312626741305 6.390989216483896E-5 82-83 0.0 0.0 0.0 1.5026493845796933 1.278197843296779E-4 84-85 0.0 0.0 0.0 1.7407776427858832 1.278197843296779E-4 86-87 0.0 0.0 0.0 2.0283721575276585 1.278197843296779E-4 88-89 0.0 0.0 0.0 2.381921680983548 1.278197843296779E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4565 0.0 36.547543 1 GTATCAA 7025 0.0 33.49275 1 GTCTACG 105 6.8751826E-5 27.161505 1 ATCAACG 8770 0.0 26.434803 3 TCAACGC 8890 0.0 26.07798 4 TATCAAC 9005 0.0 25.903212 2 CAACGCA 9060 0.0 25.588657 5 GTACATG 6360 0.0 25.036842 1 AACGCAG 9350 0.0 24.84025 6 TACATGG 6620 0.0 23.609879 2 ACATGGG 6430 0.0 23.273165 3 GGACCGT 130 2.943766E-4 21.921314 6 ACGCAGA 10645 0.0 21.552704 7 CGCAGAG 10700 0.0 21.397528 8 CATGGGG 3150 0.0 20.661692 4 GAGTACT 6140 0.0 20.578495 12-13 TGAACCG 255 1.1907105E-8 20.493132 5 CGTAGAC 140 4.8481286E-4 20.360062 3 AGTACTT 6515 0.0 19.904375 12-13 GTACTTT 6385 0.0 19.60101 14-15 >>END_MODULE