FastQCFastQC Report
Thu 26 May 2016
SRR937360_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937360_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648017
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57690.35005706858606433No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44490.2699608074431271No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT30960.18786213977161642No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN25380.15400326574301115No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA70650.036.726311
GGTATCA42800.036.6409721
ATCAACG86700.029.3731143
TCAACGC87100.029.1863354
TATCAAC88350.028.9858842
CAACGCA88000.028.887845
AACGCAG90300.028.199526
ACGCAGA102650.024.712727
CGCAGAG102950.024.6384568
GTACATG65800.023.1833381
GCAGAGT109750.022.2016379
TACATGG66850.022.0325412
GAGTACT53800.021.98311812-13
ACATGGG67400.020.7248673
AGTACTT58050.020.41458312-13
GTACTTT58150.020.37947714-15
AGAGTAC95100.020.30262810-11
CCGAACG650.007576165718.26499784-85
CAGAGTA108450.017.91290310-11
GTCTTAG7000.017.6511151
Produced by FastQC (version 0.11.5)