FastQCFastQC Report
Thu 26 May 2016
SRR937360_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937360_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1648017
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT65690.39860025715754144No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53060.321962698200322No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39230.23804366095738091No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49550.036.3621831
GTATCAA76750.033.571851
TCAACGC95000.026.7986744
ATCAACG95150.026.7564243
CAACGCA96250.026.450645
TATCAAC97850.026.1152112
AACGCAG100050.025.492726
GTACATG67400.024.192941
TACATGG68950.022.526082
ACGCAGA112200.022.442637
GAGTACT61100.022.27789312-13
CGCAGAG112450.022.2659828
ACATGGG66700.021.8617363
AGTACTT65300.020.9177712-13
GTACTTT65350.020.49568614-15
GCAGAGT120450.020.1954659
CATGGGG30900.019.982834
AGAGTAC103000.019.37326210-11
GGACCGA4550.018.789716
GGCCCGT1801.234488E-418.4707476
Produced by FastQC (version 0.11.5)