##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937360_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1648017 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.18329240535747 33.0 31.0 34.0 31.0 34.0 2 32.38843045915182 34.0 31.0 34.0 31.0 34.0 3 32.447333370954304 34.0 31.0 34.0 31.0 34.0 4 36.09478543000466 37.0 35.0 37.0 35.0 37.0 5 36.01172378682987 37.0 35.0 37.0 35.0 37.0 6 36.05068212281791 37.0 35.0 37.0 35.0 37.0 7 35.99286051054085 37.0 35.0 37.0 35.0 37.0 8 35.96507499619239 37.0 35.0 37.0 35.0 37.0 9 37.6697825325831 39.0 37.0 39.0 35.0 39.0 10-11 37.590273947416804 39.0 37.0 39.0 35.0 39.0 12-13 37.49761137172735 39.0 37.0 39.0 35.0 39.0 14-15 38.70048397559006 40.0 38.0 41.0 34.5 41.0 16-17 38.664232225759804 40.0 38.0 41.0 34.0 41.0 18-19 38.65370411834344 40.0 38.0 41.0 34.0 41.0 20-21 38.55485289290098 40.0 38.0 41.0 34.0 41.0 22-23 38.41110467913863 40.0 38.0 41.0 34.0 41.0 24-25 38.36264371059279 40.0 38.0 41.0 34.0 41.0 26-27 38.31955222549282 40.0 38.0 41.0 34.0 41.0 28-29 38.20502519088092 40.0 38.0 41.0 34.0 41.0 30-31 38.282709462341714 40.0 38.0 41.0 34.0 41.0 32-33 38.23549271639795 40.0 38.0 41.0 34.0 41.0 34-35 38.12573687043277 40.0 38.0 41.0 33.5 41.0 36-37 37.97258584104412 40.0 37.0 41.0 33.0 41.0 38-39 37.85948658296607 40.0 37.0 41.0 33.0 41.0 40-41 37.69257416640726 40.0 37.0 41.0 33.0 41.0 42-43 37.49810499527614 40.0 37.0 41.0 33.0 41.0 44-45 37.229863526893226 40.0 37.0 41.0 32.0 41.0 46-47 36.9186628535992 40.0 36.0 41.0 31.0 41.0 48-49 36.62554148409877 39.5 36.0 41.0 30.5 41.0 50-51 36.40135174576476 39.0 36.0 41.0 30.0 41.0 52-53 36.17437259445746 39.0 35.5 40.5 30.0 41.0 54-55 35.95009851233331 39.0 35.0 40.0 29.0 41.0 56-57 35.693715841523485 39.0 35.0 40.0 28.5 41.0 58-59 35.43490783165465 38.0 35.0 40.0 28.0 41.0 60-61 35.119672005810614 38.0 34.0 40.0 28.0 41.0 62-63 34.7583107455809 38.0 34.0 40.0 26.5 41.0 64-65 34.36087673852879 37.0 34.0 40.0 26.0 41.0 66-67 33.95185213502045 36.5 33.0 39.0 26.0 41.0 68-69 33.5164367236503 36.0 33.0 39.0 25.5 40.0 70-71 33.11815958209169 35.5 32.5 38.5 25.0 40.0 72-73 32.642949981705286 35.0 32.0 38.0 23.5 40.0 74-75 32.164974633150024 35.0 31.5 37.0 22.5 39.0 76-77 30.887470214202885 34.0 30.0 36.0 21.5 38.5 78-79 31.49641174817978 34.5 31.0 36.0 22.0 39.0 80-81 31.314580189403387 34.5 31.0 36.0 21.0 37.5 82-83 30.964629309042323 34.0 31.0 35.5 20.0 37.0 84-85 30.60236150476603 34.0 31.0 35.0 19.0 37.0 86-87 30.19025107143919 34.0 30.0 35.0 17.5 36.0 88-89 29.725896941597085 34.0 30.0 35.0 10.5 36.0 90-91 29.221799289691795 34.0 29.0 35.0 6.5 35.0 92-93 28.866352106804722 34.0 29.0 35.0 2.0 35.0 94-95 28.423409770651638 34.0 29.0 35.0 2.0 35.0 96-97 27.94937400524388 33.0 29.0 35.0 2.0 35.0 98-99 27.188651876770688 33.0 27.0 35.0 2.0 35.0 100-101 24.688994106250117 31.0 21.5 33.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 -0.0857812272488303 1101 2 -0.07717183580518139 1101 3 0.020347242051037995 1101 4 0.04208620760034876 1101 5 -0.04192667499374636 1101 6 -0.07818816132785855 1101 7 -0.08188502385074514 1101 8 0.04361099973750271 1101 9 -0.050063175937502535 1101 10-11 0.037735972540126284 1101 12-13 -0.08238549475131407 1101 14-15 -0.15667671166318087 1101 16-17 -0.06883131554916133 1101 18-19 0.05667139515625763 1101 20-21 0.09640191052885427 1101 22-23 0.026642574316987577 1101 24-25 -0.1134430884795492 1101 26-27 -0.10860104750644695 1101 28-29 -0.22873196281624786 1101 30-31 -0.21542429938327956 1101 32-33 -0.08083990435255117 1101 34-35 -0.03530236710350465 1101 36-37 -0.0921457006451476 1101 38-39 -0.07960042584785754 1101 40-41 0.018148582005764524 1101 42-43 -0.10026740210933838 1101 44-45 -0.12118799371812372 1101 46-47 -0.06967668838459673 1101 48-49 0.007367832186140788 1101 50-51 -0.025754976295424115 1101 52-53 0.10430569543849089 1101 54-55 -0.05785403651723442 1101 56-57 -0.007562822579629369 1101 58-59 0.024214694894929778 1101 60-61 -0.02579010913986224 1101 62-63 -0.21663871026765236 1101 64-65 -0.13646663448884766 1101 66-67 0.05096404101717411 1101 68-69 -0.09571225915404113 1101 70-71 -0.01155411492592151 1101 72-73 0.05185205857663533 1101 74-75 0.047131589629906046 1101 76-77 0.13980731489656506 1101 78-79 -0.038780811463052345 1101 80-81 -0.1881760139011348 1101 82-83 -0.1725036257172654 1101 84-85 -0.014156984730668398 1101 86-87 -0.102064856398421 1101 88-89 -0.015145875718118873 1101 90-91 0.03851679509822503 1101 92-93 -0.02771922871653132 1101 94-95 -0.0443374041652298 1101 96-97 -0.19689619681825832 1101 98-99 0.03453697999940886 1101 100-101 0.789698269894501 1102 1 0.001549853832251813 1102 2 -0.009617900290720627 1102 3 0.019180219765594586 1102 4 -0.00369319021852732 1102 5 -0.02966493957411842 1102 6 0.004884384902581473 1102 7 -0.007084763063637922 1102 8 0.04291635772707281 1102 9 -0.04269238409303 1102 10-11 -0.06497028635033786 1102 12-13 -0.04159631741608649 1102 14-15 0.06201609298356914 1102 16-17 -0.055020860357124945 1102 18-19 -0.07506698796370159 1102 20-21 0.11180820498119459 1102 22-23 -0.05991503592483127 1102 24-25 -0.09196015818083225 1102 26-27 -0.06929687491280134 1102 28-29 -0.12877048818372572 1102 30-31 -0.24948368771898544 1102 32-33 -0.10690438989072248 1102 34-35 -0.05421446999587687 1102 36-37 -0.13133459348536292 1102 38-39 -0.07458738649270913 1102 40-41 -0.007350469677497529 1102 42-43 -0.05081861121317388 1102 44-45 -0.06068242235729571 1102 46-47 -0.08234116206881481 1102 48-49 -0.1412336969745951 1102 50-51 -0.22095916074302835 1102 52-53 -0.10551530977725321 1102 54-55 -0.24941312850396002 1102 56-57 -0.26921704258910495 1102 58-59 -0.34655047817287965 1102 60-61 -0.37792174735702844 1102 62-63 -0.3602506116427051 1102 64-65 -0.35362524046324495 1102 66-67 -0.2178072961094344 1102 68-69 -0.2774859078026921 1102 70-71 -0.1860938849591065 1102 72-73 -0.18163507987760852 1102 74-75 -0.2631123055810889 1102 76-77 -0.2932453759521785 1102 78-79 -0.3426499437532371 1102 80-81 -0.33944883277263926 1102 82-83 -0.24841578787942709 1102 84-85 -0.3503651995244077 1102 86-87 -0.30197980424100024 1102 88-89 -0.20623851440944208 1102 90-91 -0.13227297493496337 1102 92-93 -0.049155039527342126 1102 94-95 0.14614742276696546 1102 96-97 0.12066988900440379 1102 98-99 0.06356110280642824 1102 100-101 0.8279957450011857 1103 1 -0.015633109862342565 1103 2 0.012836979838581897 1103 3 -0.03368500426242349 1103 4 -0.046199550399300904 1103 5 0.0940236659749587 1103 6 0.004606033461442394 1103 7 -0.06009544816734547 1103 8 -0.0958145991434165 1103 9 -0.06926087949872795 1103 10-11 -0.045083722045170305 1103 12-13 -0.03773261983145915 1103 14-15 -0.04428362866498503 1103 16-17 -0.04416754857358285 1103 18-19 -0.07845748809839392 1103 20-21 -0.1783665877517322 1103 22-23 -0.1901056916968784 1103 24-25 -0.06987850842475751 1103 26-27 -0.10887425960645913 1103 28-29 -0.3350169339650577 1103 30-31 -0.22572803838193778 1103 32-33 -0.09389579990538266 1103 34-35 0.0885747311654228 1103 36-37 -0.009962590252889925 1103 38-39 0.024413242042804484 1103 40-41 0.07652490070949369 1103 42-43 0.07901437792213528 1103 44-45 0.053851713107079036 1103 46-47 0.05492351124237871 1103 48-49 -0.11654751577678724 1103 50-51 0.06699779953951435 1103 52-53 0.0975555351540649 1103 54-55 0.07181880110005778 1103 56-57 -0.08840264014679633 1103 58-59 -0.1360314874213273 1103 60-61 -0.062037158449186336 1103 62-63 -0.06704597843807392 1103 64-65 -0.24204671841960845 1103 66-67 0.11021281702695518 1103 68-69 0.0015503377451864253 1103 70-71 -0.08840958936784205 1103 72-73 0.04361289234710597 1103 74-75 0.1792578719053708 1103 76-77 0.3002693347718335 1103 78-79 0.16872924274222711 1103 80-81 0.03054757559586463 1103 82-83 0.054344380927251024 1103 84-85 0.040612023545836706 1103 86-87 0.26300388376594697 1103 88-89 0.43087560968142213 1103 90-91 0.3330968134580772 1103 92-93 0.41249741026464193 1103 94-95 0.46038734375106927 1103 96-97 0.6698737440222118 1103 98-99 1.087448145995797 1103 100-101 1.4353813365728243 1104 1 -0.05988248959789644 1104 2 0.08178388568845207 1104 3 -0.01097256748928288 1104 4 -0.13832872586393563 1104 5 -0.04415095138175218 1104 6 -0.12486907876964892 1104 7 -0.04850583287110055 1104 8 0.040329951342641834 1104 9 0.017587083079966703 1104 10-11 -0.010414556086594473 1104 12-13 -0.07807746376550284 1104 14-15 -0.11490442797646949 1104 16-17 -0.19850915655672452 1104 18-19 -0.1264023189038639 1104 20-21 -0.1880066702672636 1104 22-23 -0.20612650535255028 1104 24-25 -0.07611063137630225 1104 26-27 -0.1278407975401521 1104 28-29 0.013312758619612453 1104 30-31 -0.19558093754839234 1104 32-33 -0.19838954134294084 1104 34-35 -0.08980767022134017 1104 36-37 -0.10681683752962101 1104 38-39 -0.12126107445070033 1104 40-41 -0.17370849620018447 1104 42-43 -0.10970252256690571 1104 44-45 -0.11063050435806332 1104 46-47 -0.10961887886354305 1104 48-49 -0.09674798184124 1104 50-51 -0.03712761239995643 1104 52-53 0.13865800733788802 1104 54-55 0.06580062531572395 1104 56-57 -0.06278277077613126 1104 58-59 -0.14677595062666882 1104 60-61 -0.1140302708091312 1104 62-63 -0.010475490747936078 1104 64-65 0.045191029154345586 1104 66-67 -0.02996026136887764 1104 68-69 -0.11415010218367883 1104 70-71 -0.20156515205496817 1104 72-73 -0.15124465652341712 1104 74-75 -0.1279739070288599 1104 76-77 0.05725422353754084 1104 78-79 -0.15350436568339987 1104 80-81 -0.21241587225998515 1104 82-83 -0.2511603216557923 1104 84-85 -0.29227548522174374 1104 86-87 -0.22047477561192963 1104 88-89 -0.17293860637847303 1104 90-91 -0.16339737046111935 1104 92-93 0.06628804351178985 1104 94-95 0.4064817746819962 1104 96-97 0.35313347223637237 1104 98-99 0.451146174156591 1104 100-101 0.842668364931761 1105 1 -0.010385221739191763 1105 2 -0.05305174736093221 1105 3 -0.060529407216762365 1105 4 -0.012306100649617235 1105 5 -0.09550069268259875 1105 6 -0.10366300546299101 1105 7 -0.0024492184280973106 1105 8 -0.08195446350737967 1105 9 -0.09873288740625696 1105 10-11 0.001214658925512424 1105 12-13 0.012832987922308803 1105 14-15 0.05957174781195107 1105 16-17 -0.05504543038169629 1105 18-19 0.058587989327634205 1105 20-21 -0.021292829938012403 1105 22-23 -0.03202061257586308 1105 24-25 -0.08818754259002759 1105 26-27 -0.06589951060635002 1105 28-29 -0.08849798427485922 1105 30-31 -0.04258918991539673 1105 32-33 0.03484082639995023 1105 34-35 -0.08772053804739244 1105 36-37 0.019403251797939447 1105 38-39 -0.12716277179536917 1105 40-41 -0.18104490791738925 1105 42-43 -0.08063024102480654 1105 44-45 -0.11702223324255812 1105 46-47 -0.18468275986495541 1105 48-49 -0.0745305475441782 1105 50-51 -0.038148498841458434 1105 52-53 -0.05511325482974172 1105 54-55 0.08985647167472877 1105 56-57 0.013512463776763184 1105 58-59 0.0720371176874508 1105 60-61 0.09102471977125504 1105 62-63 0.26825508413570986 1105 64-65 0.04241005102659301 1105 66-67 0.1500007598804416 1105 68-69 0.1955153579258493 1105 70-71 0.15888638456661397 1105 72-73 0.38273912904205076 1105 74-75 0.28766053337356823 1105 76-77 0.4425054988896058 1105 78-79 0.40605752404514206 1105 80-81 0.1603129124436542 1105 82-83 0.18236896381441525 1105 84-85 0.14408346401516425 1105 86-87 0.21399964628390933 1105 88-89 0.34477555751371725 1105 90-91 0.46262464996265606 1105 92-93 0.4144381404294748 1105 94-95 0.369445762428942 1105 96-97 0.2881965065310226 1105 98-99 0.6282167501893454 1105 100-101 1.140415520148231 1106 1 -0.12902617674377836 1106 2 -0.027248257921073105 1106 3 -0.09769908378037684 1106 4 -0.040986847512179736 1106 5 -0.07228877553128399 1106 6 -0.053555873537675325 1106 7 -0.17719669317556708 1106 8 -0.14524392376654305 1106 9 -0.185621980355954 1106 10-11 -0.23321550126222235 1106 12-13 -0.18404998320308863 1106 14-15 -0.2185223242215102 1106 16-17 -0.12732376932670064 1106 18-19 0.017240167676789042 1106 20-21 -0.03970421653122003 1106 22-23 0.004919495576366728 1106 24-25 -0.17578064533464755 1106 26-27 -0.16143568038494038 1106 28-29 -0.28225882500539257 1106 30-31 -0.15804679628208618 1106 32-33 -0.09918121216754372 1106 34-35 -0.08551668129808121 1106 36-37 -0.0460441515282497 1106 38-39 -0.25525684382883185 1106 40-41 -0.11138875038244578 1106 42-43 -0.2921902859181813 1106 44-45 -0.0900709017457757 1106 46-47 -0.15456338429103766 1106 48-49 -0.282014477755375 1106 50-51 -0.17678741323795322 1106 52-53 0.006599835671231347 1106 54-55 -0.08716117958534397 1106 56-57 -0.147476210848275 1106 58-59 -0.08433978262885233 1106 60-61 -0.12188722798917695 1106 62-63 -0.036101614160379825 1106 64-65 -0.07777666461466737 1106 66-67 -0.15656999820546247 1106 68-69 -0.04911784276795572 1106 70-71 0.06352876799687124 1106 72-73 0.02441854950935607 1106 74-75 0.09124690211145037 1106 76-77 0.19471955867942015 1106 78-79 0.44463522019859525 1106 80-81 0.12132709466995806 1106 82-83 0.09457690177992717 1106 84-85 0.06307642391721302 1106 86-87 0.07661060434941191 1106 88-89 0.4433471318428701 1106 90-91 0.20875259609060492 1106 92-93 0.28564005860109276 1106 94-95 0.20355452684073683 1106 96-97 0.2990270340282173 1106 98-99 0.5703400029186589 1106 100-101 1.1888238458292832 1107 1 -0.05679150122143284 1107 2 -0.10857384253433366 1107 3 -0.035251472200343414 1107 4 -0.06922447492789274 1107 5 -0.10930951565704561 1107 6 -0.16913264582677812 1107 7 -0.03745129397811553 1107 8 -0.0818744604884003 1107 9 -0.09972311345252649 1107 10-11 -0.1365845033070272 1107 12-13 -0.03987843515025702 1107 14-15 -0.08656961601309376 1107 16-17 -0.09409897957222313 1107 18-19 -0.11331208237318435 1107 20-21 -0.07409331297511557 1107 22-23 -0.016342285091802466 1107 24-25 -0.07769507117601648 1107 26-27 -0.16817506817455552 1107 28-29 -0.15750058814669643 1107 30-31 -0.28724747890743174 1107 32-33 -0.1551754006274777 1107 34-35 -0.10110821305399753 1107 36-37 -0.10660301258939597 1107 38-39 -0.10606461714802151 1107 40-41 -0.16060111758567786 1107 42-43 -0.19226992554119704 1107 44-45 -0.062074499365806446 1107 46-47 -0.22056034015100323 1107 48-49 -0.20394750667470873 1107 50-51 -0.10185882753310693 1107 52-53 -0.2399655134055365 1107 54-55 -0.10382630880754817 1107 56-57 -0.06602563200043932 1107 58-59 -0.11084148150497697 1107 60-61 -0.02092063665728716 1107 62-63 -0.018591966696391182 1107 64-65 -0.0481050627512829 1107 66-67 -0.053160868860267385 1107 68-69 0.0969161655034938 1107 70-71 0.15000755895054851 1107 72-73 0.15830198047041222 1107 74-75 0.2111157580990337 1107 76-77 0.21868724159687858 1107 78-79 0.16697982299981717 1107 80-81 0.09419796470977815 1107 82-83 0.04812993592657122 1107 84-85 0.03535860647336975 1107 86-87 0.18717976628843758 1107 88-89 0.22827833120329188 1107 90-91 0.31615723609798607 1107 92-93 0.40165577128346897 1107 94-95 0.2691166934074687 1107 96-97 0.3415547464646025 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0.30020142116889303 2316 16-17 0.19825327091700728 2316 18-19 0.14082490392819125 2316 20-21 0.29004758722057744 2316 22-23 0.3192741532643524 2316 24-25 0.23583134761067726 2316 26-27 0.5476683760524494 2316 28-29 0.4685268091135768 2316 30-31 0.364987354752067 2316 32-33 0.3334508224644921 2316 34-35 0.3101716303902293 2316 36-37 0.21697428682352182 2316 38-39 0.2272504513451281 2316 40-41 -0.030910315237342445 2316 42-43 -0.004872665267228626 2316 44-45 0.22493919926432682 2316 46-47 0.09965883793118735 2316 48-49 -0.27398273172363474 2316 50-51 -0.08842634221021228 2316 52-53 0.1967781265161861 2316 54-55 0.04674903365820171 2316 56-57 0.12295284158077635 2316 58-59 0.20554990792751227 2316 60-61 0.1758543144190341 2316 62-63 0.2883825929904944 2316 64-65 0.0039732151352112055 2316 66-67 0.09157494327281057 2316 68-69 0.12373416941738924 2316 70-71 0.27450070963548256 2316 72-73 0.19267380043126536 2316 74-75 0.33823904577025843 2316 76-77 0.28767354296673986 2316 78-79 0.2332342261309357 2316 80-81 0.3626206652968591 2316 82-83 0.29974637428273354 2316 84-85 0.3620826769234675 2316 86-87 0.18022868996749608 2316 88-89 0.1963174701465391 2316 90-91 0.19117876251677046 2316 92-93 0.48165577128346726 2316 94-95 0.12824780886735354 2316 96-97 0.30807529440981085 2316 98-99 0.5747039599492858 2316 100-101 0.19022740523284654 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 4.0 4 10.0 5 28.0 6 55.0 7 146.0 8 350.0 9 650.0 10 1184.0 11 1907.0 12 3098.0 13 4271.0 14 5563.0 15 7784.0 16 10323.0 17 14525.0 18 13182.0 19 8395.0 20 7284.0 21 7521.0 22 7986.0 23 8860.0 24 10359.0 25 12291.0 26 15253.0 27 18761.0 28 23680.0 29 29837.0 30 37158.0 31 47295.0 32 60717.0 33 79704.0 34 109191.0 35 155816.0 36 239650.0 37 368586.0 38 306593.0 39 29995.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.012257585390486 20.39358350077756 15.62814811846125 21.966010795370707 2 15.51780109064409 20.989832022363846 39.94509765372566 23.547269233266405 3 19.134814750090566 25.082568929810794 30.297017567173157 25.485598752925487 4 13.002050342927287 17.420633403660275 37.564600365166136 32.0127158882463 5 13.343183776502144 38.08638454350174 34.55903591062998 14.011395769366134 6 31.560839481631557 37.23511347273723 17.26863254444584 13.935414501185365 7 28.165182762071023 33.14431829283315 20.892381571306608 17.79811737378923 8 26.43564963225501 35.807518975835805 19.491728544062347 18.265102847846837 9 26.20421998074049 16.89054178445975 20.55986072959199 36.34537750520778 10-11 24.632573125851405 27.423900874688638 27.378330901114374 20.565195098345583 12-13 25.513808201937536 24.510692447519304 28.23209078378109 21.74340856676207 14-15 22.571429785008284 25.937930251933082 26.415473869504986 25.075166093553648 16-17 21.71018260127171 29.347361101250772 27.132123030284276 21.810333267193236 18-19 21.684909803721688 27.7694040777492 29.669445157422526 20.87624096110659 20-21 22.37507937572227 26.687323822303966 30.03650145493082 20.901095347042943 22-23 22.174315770567237 26.591994495157323 29.84280676588369 21.390882968391754 24-25 21.927188195159957 26.808280572475034 29.959156602965287 21.305374629399722 26-27 21.71770404027165 27.063157460400678 29.860838054184203 21.35830044514347 28-29 21.659811239865906 27.193334565155986 29.84973280674432 21.29712138823379 30-31 21.870936384449358 27.007771195424297 29.738096090494654 21.383196329631694 32-33 21.784605377250358 27.000297933819855 29.64708495118679 21.568011737742996 34-35 22.089668977929232 27.031122858562746 29.593990838686736 21.285217324821286 36-37 22.094135586627925 26.894785337404087 29.56432778433954 21.446751291628452 38-39 21.8383439370633 27.159869053795347 29.635589697846793 21.366197311294567 40-41 22.112578499764414 27.130798909839687 29.425954686685095 21.330667903710797 42-43 21.885515857723803 27.080687142745308 29.332707856311018 21.701089143219875 44-45 22.03401246409693 27.257756080274824 29.218662347122766 21.48956910850548 46-47 22.211117967836497 27.531117700848963 28.692543826914406 21.565220504400138 48-49 22.250437950579393 27.621772105506192 28.479105494664193 21.64868444925022 50-51 22.26657856077941 27.76078766177776 28.411448425592695 21.56118535185013 52-53 22.514148822493944 27.737759986699167 28.133963423920992 21.6141277668859 54-55 22.193976154372194 27.810332053613525 28.289422985321146 21.706268806693135 56-57 22.211208986315068 27.991913918363707 28.18584395670676 21.61103313861447 58-59 22.356049725215215 27.60475165001332 28.273858825485416 21.76533979928605 60-61 22.20520176672935 27.849075585992132 28.439330419528442 21.50639222775008 62-63 22.16806622747217 27.772862779934915 28.565603388799993 21.493467603792922 64-65 22.072267502257258 27.687625605576642 28.462709099911653 21.777397792254444 66-67 22.08830370075066 27.758390841842047 28.233750015928234 21.919555441479062 68-69 22.12234461173641 27.620164112384764 28.427740733257 21.829750542621827 70-71 22.18705875000076 27.658907644763374 28.32598207421404 21.828051531021828 72-73 22.17534770575789 27.675017915470534 28.19804043283534 21.95159394593624 74-75 22.106416377986392 28.064000553392347 27.90987592967791 21.919707138943348 76-77 22.181627980779325 27.742401929106315 28.131232869563842 21.94473722055052 78-79 22.14249003499357 27.826654700770682 28.044279883035188 21.986575381200556 80-81 22.129032727837593 27.98865423636664 27.943721420180385 21.938591615615383 82-83 22.05557316941822 27.960461344987813 27.969320527970897 22.01464495762307 84-85 22.124245262382157 27.941728385173235 27.892031979242798 22.041994373201803 86-87 22.064547877843495 27.937788263106512 27.906599264449333 22.091064594600663 88-89 22.193854796400768 27.81755285291353 27.760939359242048 22.227652991443655 90-91 22.144266747902456 27.767255828404775 27.84286822204071 22.245609201652066 92-93 22.142915656582513 27.63429441766057 28.009211360678172 22.21357856507875 94-95 22.02091968711488 27.650928358141936 27.954808718599384 22.373343236143803 96-97 22.147374693343576 27.612002788806183 27.880628658563595 22.359993859286647 98-99 21.941203758343 27.719402784018456 27.722558097976744 22.6168353596618 100-101 21.92715919021264 27.66066487037577 27.907806583163413 22.50436935624818 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 298.0 1 205.0 2 154.5 3 221.5 4 204.0 5 192.5 6 369.0 7 833.5 8 1392.5 9 1691.0 10 1652.5 11 1517.0 12 1578.5 13 1757.5 14 1904.0 15 2081.0 16 2268.5 17 2392.0 18 2556.5 19 2745.0 20 3069.0 21 3509.5 22 4170.5 23 5054.0 24 5938.0 25 7416.5 26 9759.5 27 12459.0 28 14757.0 29 17402.0 30 20825.0 31 24229.0 32 28205.5 33 32096.0 34 35890.5 35 39548.0 36 44007.5 37 48235.0 38 51025.5 39 53192.0 40 54036.5 41 55445.0 42 57096.0 43 58996.0 44 61314.5 45 62520.0 46 62061.0 47 62399.5 48 64498.5 49 63559.0 50 60663.5 51 57648.0 52 54335.5 53 52564.0 54 51026.5 55 46800.0 56 41243.5 57 36755.0 58 33123.5 59 28962.5 60 23747.5 61 19033.0 62 14937.5 63 11079.5 64 7852.0 65 5655.0 66 4084.5 67 3009.0 68 2166.0 69 1453.5 70 1051.0 71 725.0 72 454.5 73 348.0 74 256.5 75 162.5 76 102.5 77 71.5 78 51.5 79 28.0 80 15.5 81 8.5 82 7.5 83 4.5 84 1.5 85 1.0 86 2.5 87 2.5 88 1.5 89 1.0 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.07281478285721567 2 0.0 3 0.0 4 0.0 5 0.0022451224714308167 6 0.0 7 0.0 8 0.0 9 0.0 10-11 1.2135797142869277E-4 12-13 0.006158917050006159 14-15 0.0 16-17 0.0 18-19 0.0 20-21 3.0339492857173193E-5 22-23 8.191663071436763E-4 24-25 9.101847857151959E-4 26-27 5.461108714291175E-4 28-29 0.0031856467500031857 30-31 4.854318857147711E-4 32-33 0.0 34-35 0.0 36-37 3.0339492857173193E-5 38-39 7.281478285721567E-4 40-41 1.51697464285866E-4 42-43 0.012621229028584051 44-45 2.1237645000021237E-4 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 6.0678985714346387E-5 66-67 0.0 68-69 0.0 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.0678985714346387E-5 82-83 5.764503642862907E-4 84-85 6.371293500006371E-4 86-87 0.0 88-89 0.0 90-91 0.008434379014294148 92-93 0.003155307257146013 94-95 0.0 96-97 0.0 98-99 2.1237645000021237E-4 100-101 0.010740180471439311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1648017.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 58.374832183646795 #Duplication Level Percentage of deduplicated Percentage of total 1 76.75107700875826 44.803312403004156 2 13.470527850572806 15.726796054046558 3 4.288463575131048 7.510150245718707 4 1.830390342055876 4.273949161923185 5 1.0093504122220238 2.9460330463977678 6 0.5929057890379007 2.0766465561480087 7 0.3834763151952998 1.5669755882150207 8 0.2748075460607452 1.283347550727663 9 0.21847993078157862 1.1478356365382476 >10 1.0806789085954627 11.3815515806756 >50 0.06623786451072665 2.6809839915984455 >100 0.032252091903894824 3.2335276625698803 >500 9.362528130380814E-4 0.3270107117080153 >1k 2.080561806751292E-4 0.32075591072269954 >5k 2.080561806751292E-4 0.7211239000060692 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6569 0.39860025715754144 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5306 0.321962698200322 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3923 0.23804366095738091 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 6.067898571434639E-5 3.0339492857173197E-5 18-19 0.0 0.0 0.0 1.2135797142869279E-4 6.067898571434639E-5 20-21 0.0 0.0 0.0 1.5169746428586597E-4 6.067898571434639E-5 22-23 0.0 0.0 0.0 3.3373442142890515E-4 6.067898571434639E-5 24-25 0.0 0.0 0.0 5.764503642862906E-4 6.067898571434639E-5 26-27 0.0 0.0 0.0 8.798452928580227E-4 6.067898571434639E-5 28-29 0.0 0.0 0.0 0.0016990116000016992 6.067898571434639E-5 30-31 0.0 0.0 0.0 0.003822776100003823 6.067898571434639E-5 32-33 0.0 0.0 0.0 0.006826385892863969 6.067898571434639E-5 34-35 0.0 0.0 0.0 0.010770519964296486 6.067898571434639E-5 36-37 0.0 0.0 0.0 0.014380919614300096 6.067898571434639E-5 38-39 0.0 0.0 0.0 0.01850709064287565 6.067898571434639E-5 40-41 0.0 0.0 0.0 0.02154103992859297 6.067898571434639E-5 42-43 0.0 0.0 0.0 0.02475702617145333 6.067898571434639E-5 44-45 0.0 0.0 0.0 0.02854946277859998 6.067898571434639E-5 46-47 0.0 0.0 0.0 0.03567924360003568 6.067898571434639E-5 48-49 0.0 0.0 0.0 0.04453837551433025 6.067898571434639E-5 50-51 0.0 0.0 0.0 0.05248732264290963 1.2135797142869279E-4 52-53 0.0 0.0 0.0 0.06777842704292492 1.2135797142869279E-4 54-55 0.0 0.0 0.0 0.09763248801438335 1.2135797142869279E-4 56-57 0.0 0.0 0.0 0.14377885665014378 1.2135797142869279E-4 58-59 0.0 0.0 0.0 0.1892577564430464 1.2135797142869279E-4 60-61 0.0 0.0 0.0 0.23106557760023105 1.2135797142869279E-4 62-63 0.0 0.0 0.0 0.28191456762885336 1.8203695714303917E-4 64-65 0.0 0.0 0.0 0.3453241077003453 1.8203695714303917E-4 66-67 0.0 0.0 0.0 0.4477805750790192 1.8203695714303917E-4 68-69 0.0 0.0 0.0 0.5731736990577161 1.8203695714303917E-4 70-71 0.0 0.0 0.0 0.6840645454506841 1.8203695714303917E-4 72-73 0.0 0.0 0.0 0.8066057571008066 1.8203695714303917E-4 74-75 0.0 0.0 0.0 0.946470819172375 2.1237645000021237E-4 76-77 0.0 0.0 0.0 1.1079072606653937 2.4271594285738557E-4 78-79 0.0 0.0 0.0 1.2559639858083989 2.4271594285738557E-4 80-81 0.0 0.0 0.0 1.4131832377942704 2.4271594285738557E-4 82-83 0.0 0.0 0.0 1.578654831837293 3.0339492857173195E-4 84-85 0.0 0.0 0.0 1.8205516083875348 3.0339492857173195E-4 86-87 0.0 0.0 0.0 2.1061675941449636 3.0339492857173195E-4 88-89 0.0 0.0 0.0 2.4486397895167347 3.0339492857173195E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4955 0.0 36.362183 1 GTATCAA 7675 0.0 33.57185 1 TCAACGC 9500 0.0 26.798674 4 ATCAACG 9515 0.0 26.756424 3 CAACGCA 9625 0.0 26.45064 5 TATCAAC 9785 0.0 26.115211 2 AACGCAG 10005 0.0 25.49272 6 GTACATG 6740 0.0 24.19294 1 TACATGG 6895 0.0 22.52608 2 ACGCAGA 11220 0.0 22.44263 7 GAGTACT 6110 0.0 22.277893 12-13 CGCAGAG 11245 0.0 22.265982 8 ACATGGG 6670 0.0 21.861736 3 AGTACTT 6530 0.0 20.91777 12-13 GTACTTT 6535 0.0 20.495686 14-15 GCAGAGT 12045 0.0 20.195465 9 CATGGGG 3090 0.0 19.98283 4 AGAGTAC 10300 0.0 19.373262 10-11 GGACCGA 455 0.0 18.78971 6 GGCCCGT 180 1.234488E-4 18.470747 6 >>END_MODULE