FastQCFastQC Report
Thu 26 May 2016
SRR937359_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937359_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331367
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19710.5948087769753777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15400.46474151016848386No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10720.32350837590949005No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6550.1976660319223097No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3910.11799605875056961No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3730.11256401512522368No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9000.044.3778651
GTATCAA17300.042.600551
GAAGCGA459.6177444E-442.2198686
ATCAACG22000.033.2682843
TATCAAC22150.033.2575532
TCAACGC22100.033.1127364
CAACGCA22500.032.7303055
AACGCAG22800.032.4981886
CCGACGC701.7071216E-830.52939294-95
GTACATG14550.030.3913211
CCTTAGA953.4595985E-530.0301361
GTACTAT650.00583971729.2291416
TACATGG14850.029.1237492
ACGCAGA25750.028.959557
CGCAGAG26050.027.8967178
TAACCGA350.00834804927.1701250-51
GCAGAGT26550.027.0135619
ACATGGG15650.026.7239513
CATGGGA9650.025.606054
GTATAAC1151.2742878E-424.7962343
Produced by FastQC (version 0.11.5)