##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937359_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 331367 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.697863697954233 33.0 31.0 34.0 30.0 34.0 2 31.82813315749607 33.0 31.0 34.0 30.0 34.0 3 31.846342574849036 33.0 31.0 34.0 30.0 34.0 4 35.461099023137486 37.0 35.0 37.0 33.0 37.0 5 35.46225785911089 37.0 35.0 37.0 33.0 37.0 6 35.50417814688849 37.0 35.0 37.0 33.0 37.0 7 35.44792028174199 37.0 35.0 37.0 33.0 37.0 8 35.426312819321176 37.0 35.0 37.0 33.0 37.0 9 37.02410620248848 39.0 37.0 39.0 33.0 39.0 10-11 36.92123385853148 39.0 37.0 39.0 33.0 39.0 12-13 36.789162771187236 39.0 37.0 39.0 33.0 39.0 14-15 37.89364511251875 40.0 38.0 41.0 33.0 41.0 16-17 37.826728370658515 40.0 38.0 41.0 33.0 41.0 18-19 37.77476332887704 40.0 38.0 41.0 33.0 41.0 20-21 37.672758602999096 40.0 37.5 41.0 32.0 41.0 22-23 37.71414926652322 40.0 38.0 41.0 32.5 41.0 24-25 37.670419806438176 40.0 37.0 41.0 32.0 41.0 26-27 37.57610745789412 40.0 37.0 41.0 32.0 41.0 28-29 37.416925946156375 40.0 37.0 41.0 32.0 41.0 30-31 37.14861015128241 39.5 36.5 41.0 31.5 41.0 32-33 36.871144380701764 39.0 36.0 41.0 31.0 41.0 34-35 36.8940645869987 39.0 36.0 41.0 31.0 41.0 36-37 36.7357914336672 39.0 36.0 41.0 30.5 41.0 38-39 36.58436567310565 39.0 35.5 41.0 30.0 41.0 40-41 36.34945091092354 39.0 35.0 41.0 30.0 41.0 42-43 36.06225121994646 39.0 35.0 41.0 29.5 41.0 44-45 35.78227916479311 39.0 35.0 40.5 28.5 41.0 46-47 35.322504956739806 39.0 35.0 40.0 26.5 41.0 48-49 34.9294045574846 38.5 35.0 40.0 24.5 41.0 50-51 33.9331753011012 37.5 33.5 39.5 23.5 40.5 52-53 34.01177848126096 38.0 33.5 39.5 23.0 40.5 54-55 34.30788219708058 38.0 34.0 40.0 23.0 41.0 56-57 34.047694851931546 38.0 34.0 40.0 21.5 41.0 58-59 33.74933683800741 38.0 33.5 40.0 20.0 41.0 60-61 33.4210256301925 37.5 33.0 40.0 18.5 41.0 62-63 33.020809253788094 37.0 32.5 40.0 16.5 41.0 64-65 32.596151698871644 36.0 32.0 39.0 12.5 41.0 66-67 32.13404171205944 36.0 31.5 39.0 8.0 40.5 68-69 31.62017792960675 35.0 31.0 39.0 7.0 40.0 70-71 31.14864334710457 35.0 30.5 38.0 2.0 40.0 72-73 30.654451107080668 35.0 30.0 37.5 2.0 39.5 74-75 30.11466289642602 34.5 29.5 37.0 2.0 39.0 76-77 29.601962476649756 34.0 29.0 36.0 2.0 39.0 78-79 29.05433552526353 34.0 28.5 36.0 2.0 38.5 80-81 28.485496443520326 34.0 27.5 35.0 2.0 37.0 82-83 27.910227934586125 34.0 26.5 35.0 2.0 37.0 84-85 27.391546231217955 33.0 26.0 35.0 2.0 36.0 86-87 26.820476993786347 33.0 25.0 35.0 2.0 36.0 88-89 26.276409539875726 32.5 24.5 35.0 2.0 35.5 90-91 25.68843457556124 32.0 21.5 35.0 2.0 35.0 92-93 25.01778239836797 32.0 19.0 34.0 2.0 35.0 94-95 24.252683580441023 31.0 11.5 34.0 2.0 35.0 96-97 23.390844592249678 31.0 2.0 34.0 2.0 35.0 98-99 22.19322080955557 30.5 2.0 34.0 2.0 35.0 100-101 19.242887795103314 26.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 -0.32941854168064566 1101 2 -0.20512239673772115 1101 3 -0.20229439557996542 1101 4 -0.37990140360862057 1101 5 -0.4339260136576044 1101 6 -0.46619479964125077 1101 7 -0.2922077836515129 1101 8 -0.16886596041432966 1101 9 -0.0187586049220414 1101 10-11 -0.18418846725263194 1101 12-13 -0.1315608507248882 1101 14-15 -0.14389781151552938 1101 16-17 -0.2944812219457944 1101 18-19 -0.2203933385678809 1101 20-21 -0.27004982092579866 1101 22-23 -0.32303613020649635 1101 24-25 -0.4656220444154471 1101 26-27 -0.2575456178346798 1101 28-29 -0.2264985038876759 1101 30-31 -0.2430603242195346 1101 32-33 -0.5672666955982848 1101 34-35 -0.19454818450294198 1101 36-37 -0.18282932342035707 1101 38-39 0.008345355602202176 1101 40-41 -0.0650578107251647 1101 42-43 -0.09988639032626878 1101 44-45 0.05294783735941877 1101 46-47 0.2999823570307356 1101 48-49 0.4702115227235595 1101 50-51 0.5955429199869187 1101 52-53 0.120308685987915 1101 54-55 0.3085786847477294 1101 56-57 0.3328802021365931 1101 58-59 0.4463705391894237 1101 60-61 0.19825636603968633 1101 62-63 0.41822772956155774 1101 64-65 0.5487416359621946 1101 66-67 0.7202538952240332 1101 68-69 0.5270154052923139 1101 70-71 0.401785596018275 1101 72-73 0.512591581185621 1101 74-75 0.505689764178566 1101 76-77 0.42124666801862887 1101 78-79 0.6003975981729859 1101 80-81 0.645216904664732 1101 82-83 0.5359536576475143 1101 84-85 1.0359605294370198 1101 86-87 0.9415426157543685 1101 88-89 0.6081476340258334 1101 90-91 0.5230459914601866 1101 92-93 0.6106506742180109 1101 94-95 0.9582822352491043 1101 96-97 1.360709929661219 1101 98-99 1.3949660883975703 1101 100-101 1.4258553784990653 1102 1 0.17108734832441286 1102 2 0.0462447107759516 1102 3 0.06817608212474013 1102 4 0.02076348039803122 1102 5 0.14516145713327688 1102 6 0.2792943478136394 1102 7 -0.1372849921022805 1102 8 0.23796596365399125 1102 9 0.11079741637351503 1102 10-11 0.1696273887855213 1102 12-13 0.3417052715077702 1102 14-15 0.5016660149401062 1102 16-17 0.3917525474165444 1102 18-19 0.46788808081493727 1102 20-21 0.14797793075448595 1102 22-23 0.3709967767338256 1102 24-25 0.4818858450596295 1102 26-27 0.5373159851139349 1102 28-29 0.27266436860394805 1102 30-31 0.05548704876594002 1102 32-33 0.4429233745036143 1102 34-35 0.679141921733958 1102 36-37 0.5008810184167558 1102 38-39 0.5514544110332906 1102 40-41 0.6269696036951018 1102 42-43 0.688398642556578 1102 44-45 0.6305537731354818 1102 46-47 0.8432600424635126 1102 48-49 0.9749391852708342 1102 50-51 1.0574746971062368 1102 52-53 0.9167060264518909 1102 54-55 1.3490739751526846 1102 56-57 1.257394186381731 1102 58-59 1.4986819747125395 1102 60-61 1.479308376350204 1102 62-63 1.5898208012774901 1102 64-65 1.54580867843287 1102 66-67 1.5841514163630137 1102 68-69 1.718777829709932 1102 70-71 1.6641196761416097 1102 72-73 1.3293931518536368 1102 74-75 1.3751038953727104 1102 76-77 1.754857036354732 1102 78-79 1.922480901393822 1102 80-81 1.6196212794087792 1102 82-83 1.5272330775603074 1102 84-85 1.3320567448979794 1102 86-87 1.5287484166264242 1102 88-89 1.455784405002202 1102 90-91 1.6835395955651258 1102 92-93 1.6430035445415392 1102 94-95 1.5389777135560578 1102 96-97 1.0356334439104522 1102 98-99 1.4442120713900302 1102 100-101 1.4118530658590416 1103 1 -0.12253719060006674 1103 2 -0.03634896035586621 1103 3 0.010944476611246756 1103 4 0.04659924044462116 1103 5 -0.0066381934200165915 1103 6 -0.002716912135880989 1103 7 -0.045959035977254814 1103 8 -0.19016837919205898 1103 9 8.148887089873824E-4 1103 10-11 -0.09803131826165412 1103 12-13 0.13161790983042465 1103 14-15 0.03563417646213907 1103 16-17 -0.18619154110995595 1103 18-19 -0.009918170398414361 1103 20-21 -0.41812767980658094 1103 22-23 -0.5132994764208973 1103 24-25 -0.39680567115082255 1103 26-27 -0.15185504963659469 1103 28-29 -0.3551030551972545 1103 30-31 -0.17927901607583863 1103 32-33 -0.13762020926652951 1103 34-35 0.042059801757254434 1103 36-37 -0.0438598086071309 1103 38-39 0.24109331610026175 1103 40-41 0.6312367362907167 1103 42-43 0.35791524127527197 1103 44-45 0.5954052582839964 1103 46-47 0.6649587417452096 1103 48-49 0.6429881078101474 1103 50-51 0.5524271335263435 1103 52-53 0.4071971932156302 1103 54-55 1.2886488149896067 1103 56-57 1.1369806744422988 1103 58-59 1.100838837457637 1103 60-61 0.904515132319915 1103 62-63 0.9557245092953579 1103 64-65 0.9207882940414933 1103 66-67 0.933322451113547 1103 68-69 1.216166686242655 1103 70-71 1.2540226076112333 1103 72-73 1.1436292225195253 1103 74-75 1.0588585061279012 1103 76-77 0.6203278187270875 1103 78-79 0.4096290279711816 1103 80-81 0.36740811691605657 1103 82-83 0.5090036075544795 1103 84-85 1.055774469611631 1103 86-87 1.2088862539395713 1103 88-89 0.9962483925774315 1103 90-91 0.5885075629500989 1103 92-93 0.9024754917362579 1103 94-95 1.4250619690404314 1103 96-97 1.3871690680611053 1103 98-99 1.9417329411240623 1103 100-101 1.7373782066084118 1104 1 -0.07218847626403502 1104 2 -0.029247868422089596 1104 3 -0.13252153254690668 1104 4 -0.24650396490937254 1104 5 -0.0043909232544692145 1104 6 0.020692739149779982 1104 7 -0.10315523182086395 1104 8 -0.009301876902519268 1104 9 -0.30694136349249135 1104 10-11 -0.03491402343953354 1104 12-13 -0.0860678950304461 1104 14-15 -0.01899069144909049 1104 16-17 -0.02614927270558809 1104 18-19 -0.21473676849633705 1104 20-21 -0.20902588339938433 1104 22-23 -0.2074206458467458 1104 24-25 -0.5482815429366568 1104 26-27 -0.0832992201191658 1104 28-29 -0.06747713057590943 1104 30-31 -0.3840447786682972 1104 32-33 -0.3713046051805833 1104 34-35 -0.19401275900357717 1104 36-37 -0.2908869308332598 1104 38-39 -0.4749581104583811 1104 40-41 -0.38381081566419084 1104 42-43 -0.07102804861553125 1104 44-45 -0.33213965189564476 1104 46-47 -0.06530895814912441 1104 48-49 0.05706700883163052 1104 50-51 -0.30368417327182584 1104 52-53 -0.1610592351042044 1104 54-55 -0.33824445728936325 1104 56-57 -0.13256846257777255 1104 58-59 -0.30720624884035175 1104 60-61 0.03057712597965434 1104 62-63 -0.4224121585454341 1104 64-65 -0.4932765175118732 1104 66-67 -0.22637110295478635 1104 68-69 -0.3102334546520318 1104 70-71 -0.5094539686480104 1104 72-73 -0.3550745464131957 1104 74-75 -0.45436093733810523 1104 76-77 -0.5146880319245533 1104 78-79 -0.44399901007038167 1104 80-81 -0.27468416910014426 1104 82-83 -0.23726268339303402 1104 84-85 -0.387138527922712 1104 86-87 -0.5877710197483488 1104 88-89 -0.2524377645201419 1104 90-91 -0.3864343179938956 1104 92-93 0.02415213844284736 1104 94-95 -0.45705497354498803 1104 96-97 -0.5059764056972575 1104 98-99 -0.25269115186642566 1104 100-101 0.09633558596733849 1105 1 0.10733419881591288 1105 2 0.09308810479277341 1105 3 -0.008157357670853571 1105 4 -0.00219688015415187 1105 5 0.10374763833203815 1105 6 0.2780168007040942 1105 7 0.05660052548173411 1105 8 0.18675761701287996 1105 9 0.07236454082789834 1105 10-11 0.28933532732131795 1105 12-13 -0.07007300309388143 1105 14-15 0.18322852602992157 1105 16-17 0.16428705893221007 1105 18-19 0.4787472312461105 1105 20-21 0.4368543990464744 1105 22-23 0.5126714280699289 1105 24-25 0.38624724894194884 1105 26-27 0.18760520758456778 1105 28-29 0.28443732009264266 1105 30-31 0.6012480764816175 1105 32-33 0.9157932421355426 1105 34-35 0.9606724941460385 1105 36-37 0.8053453358162344 1105 38-39 0.402185451879312 1105 40-41 0.3459447270138867 1105 42-43 -0.06908033114724077 1105 44-45 0.5997063335528097 1105 46-47 0.20917969894529165 1105 48-49 0.09994362119829958 1105 50-51 0.3132325309541315 1105 52-53 0.6272351438797656 1105 54-55 0.688395810562028 1105 56-57 0.5370439255491988 1105 58-59 0.4744884263254434 1105 60-61 0.3936949763005231 1105 62-63 0.12199901909927746 1105 64-65 0.25193735581674304 1105 66-67 0.4481991114551036 1105 68-69 0.3891795473010511 1105 70-71 0.7823548867594496 1105 72-73 0.5148415354016684 1105 74-75 0.2554935472411195 1105 76-77 0.2342388455033877 1105 78-79 0.2678622137186011 1105 80-81 0.3453123969076266 1105 82-83 0.5663934452099717 1105 84-85 0.41753706712375305 1105 86-87 0.33517341396487055 1105 88-89 0.8709606000410623 1105 90-91 1.0792686846030612 1105 92-93 0.9574575705893054 1105 94-95 0.5942671134637898 1105 96-97 1.0586470355922053 1105 98-99 1.834387379243399 1105 100-101 1.6647364187104543 1106 1 0.027438799796090763 1106 2 -0.6989900167784384 1106 3 -0.3106041428365316 1106 4 -0.2029685866862252 1106 5 -0.3096273890982886 1106 6 0.05264923432270052 1106 7 -0.21986059660150659 1106 8 -0.24921999984031373 1106 9 0.3458449007137858 1106 10-11 -0.6911525667610547 1106 12-13 -0.1957770481527703 1106 14-15 -0.5101461356627084 1106 16-17 -0.18823835983744885 1106 18-19 -0.272295121178729 1106 20-21 3.558549954618684E-5 1106 22-23 -0.2953162831435847 1106 24-25 0.26885459295482406 1106 26-27 0.08612214123975548 1106 28-29 -0.29638156660181636 1106 30-31 -0.9701073474083728 1106 32-33 -0.9606514217515212 1106 34-35 -0.34598941793061755 1106 36-37 -0.7396221135291015 1106 38-39 -0.32915278233910783 1106 40-41 -0.46325906202861233 1106 42-43 -0.4013164514017902 1106 44-45 -0.5561253539609012 1106 46-47 -0.6382345355657151 1106 48-49 -0.3536625524539545 1106 50-51 -0.6980230465456785 1106 52-53 -0.9409840792933792 1106 54-55 -1.1790882798483295 1106 56-57 -1.4457023987869917 1106 58-59 -1.3210459164439214 1106 60-61 -1.0989902698409466 1106 62-63 -0.4868445183156922 1106 64-65 -0.3534133866307805 1106 66-67 -0.12026698466076269 1106 68-69 0.19002623006017672 1106 70-71 -0.2726520622567108 1106 72-73 -0.5630514497493806 1106 74-75 -0.5613815899105141 1106 76-77 -0.1168510528215343 1106 78-79 -0.1372197115246152 1106 80-81 0.01785829698808783 1106 82-83 -0.3484993936459979 1106 84-85 -0.04366730709905298 1106 86-87 0.08284183617245944 1106 88-89 0.29283902883193136 1106 90-91 -0.24738079239203614 1106 92-93 -0.2242592021412939 1106 94-95 0.011034357996010158 1106 96-97 0.003219488229174061 1106 98-99 -0.18532588240534764 1106 100-101 -0.0865893804264033 1107 1 -0.0033524640778814785 1107 2 0.06747972901461807 1107 3 0.23406109167832767 1107 4 -0.3504802229196713 1107 5 0.08931639894324661 1107 6 0.14502302594461014 1107 7 -0.07431816910419542 1107 8 -0.4101580592746146 1107 9 -0.22092917733848338 1107 10-11 -0.3387537124824931 1107 12-13 -0.159749479230463 1107 14-15 -0.3683183519176296 1107 16-17 -0.10486027176010992 1107 18-19 -0.34475305171936554 1107 20-21 -0.18225048682412393 1107 22-23 -0.006782448557117959 1107 24-25 -0.3778067031425607 1107 26-27 -0.07602429628262541 1107 28-29 -0.023735666136367684 1107 30-31 -0.07845858263929983 1107 32-33 -0.4379607665420693 1107 34-35 -0.21481326325822891 1107 36-37 -0.08566579415924735 1107 38-39 0.37497360505795996 1107 40-41 -0.05129164348224435 1107 42-43 -0.1725749548031601 1107 44-45 -0.010296496101972252 1107 46-47 0.7204747376172378 1107 48-49 0.4899643720000384 1107 50-51 0.5820968961682453 1107 52-53 0.6085594223177893 1107 54-55 0.5497427262383212 1107 56-57 0.09363702119152606 1107 58-59 0.042541623835347764 1107 60-61 0.8682045028049572 1107 62-63 1.0671744576957636 1107 64-65 0.558554031915584 1107 66-67 0.29398292582975216 1107 68-69 1.1945464592044708 1107 70-71 0.7309730720075187 1107 72-73 0.9460337632832001 1107 74-75 0.7008812099462709 1107 76-77 0.9155961365805432 1107 78-79 0.5510634964954342 1107 80-81 0.35835238936187963 1107 82-83 0.6279148562644927 1107 84-85 -0.20277042202923568 1107 86-87 0.36184291028631677 1107 88-89 0.05644447100879901 1107 90-91 0.06290384777982183 1107 92-93 0.8405456671748546 1107 94-95 0.644045457172524 1107 96-97 0.258232019557493 1107 98-99 0.4155513105771078 1107 100-101 0.6562946152771403 1108 1 -0.2157584791154754 1108 2 -0.004262125621973922 1108 3 -0.30040006120388085 1108 4 0.0019633861028864885 1108 5 0.03731138641191478 1108 6 -0.44394940513308256 1108 7 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3.9915992643709295 2315 96-97 3.910067794562014 2315 98-99 4.9383854578008375 2315 100-101 5.438578934322177 2316 1 -0.03194895530594977 2316 2 -0.07164307800292491 2316 3 -0.16011434691816717 2316 4 -0.2439889948494951 2316 5 0.04559710069762701 2316 6 0.2928124996288233 2316 7 0.2286291993168561 2316 8 0.12159632669029463 2316 9 0.32857959459133923 2316 10-11 -0.025560730026356282 2316 12-13 0.14679438041866177 2316 14-15 0.19575182352096476 2316 16-17 -0.0805444822864203 2316 18-19 0.04431712371923169 2316 20-21 0.41187232019342446 2316 22-23 0.016112288284986676 2316 24-25 0.11201785826094834 2316 26-27 0.3532037738928153 2316 28-29 0.3097204742145081 2316 30-31 0.20601510157122505 2316 32-33 0.3721444966158316 2316 34-35 0.05876568411019889 2316 36-37 -0.16868333801888724 2316 38-39 0.06662272786497425 2316 40-41 -0.1831162048857493 2316 42-43 -0.2789082881364848 2316 44-45 -0.2665947417160055 2316 46-47 -1.1588059641371444 2316 48-49 -1.402070715719269 2316 50-51 -1.0640434547089441 2316 52-53 -1.289861630313787 2316 54-55 -1.636292361480983 2316 56-57 -1.3607840314400477 2316 58-59 -0.9839846919541273 2316 60-61 -1.1516025147389044 2316 62-63 -1.2733343142340559 2316 64-65 -1.0662705294879231 2316 66-67 -0.8837363724158642 2316 68-69 -0.9688921372867618 2316 70-71 -1.1224479806240595 2316 72-73 -1.595194306892239 2316 74-75 -1.3252591409309211 2316 76-77 -1.2897898283317382 2316 78-79 -1.2030768543817594 2316 80-81 -0.9841212948486451 2316 82-83 -1.150172863033756 2316 84-85 -0.8114020009766065 2316 86-87 -0.8465255107663126 2316 88-89 -0.8122221956578635 2316 90-91 -1.0617277311675473 2316 92-93 -1.395524508263744 2316 94-95 -0.7957615603713357 2316 96-97 -0.5230662260565424 2316 98-99 -0.33932588240534756 2316 100-101 0.15408408896135128 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1637.0 3 289.0 4 281.0 5 304.0 6 340.0 7 392.0 8 524.0 9 609.0 10 822.0 11 1041.0 12 1320.0 13 1751.0 14 2052.0 15 2697.0 16 3269.0 17 4404.0 18 3818.0 19 2687.0 20 2701.0 21 2823.0 22 3074.0 23 3416.0 24 3682.0 25 4160.0 26 4874.0 27 5542.0 28 6435.0 29 7702.0 30 9246.0 31 11461.0 32 14127.0 33 18629.0 34 24441.0 35 33417.0 36 46398.0 37 57612.0 38 39259.0 39 4131.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.733859584290016 18.94624606977773 15.349524738439543 22.970369607492717 2 14.736682762900955 19.854074264694752 41.06961747475818 24.339625497646114 3 18.72580152024726 24.537674705834274 30.5995925422518 26.136931231666672 4 11.99417546855086 16.172762141756504 37.940586146454876 33.89247624323776 5 13.04772976344008 36.96831538093595 34.85550287904383 15.128451976580143 6 30.979364440503677 36.05306719039852 17.990370677472168 14.977197691625637 7 27.710260010652206 32.99218031278749 21.026303684694717 18.27125599186559 8 26.407083609469424 33.93462913031505 20.168072312855383 19.490214947360137 9 25.88338554198635 16.290915694375087 20.634372966510796 37.19132579712777 10-11 24.347994533641863 26.387890473070012 27.750242520457224 21.513872472830897 12-13 25.490041793899483 23.85611519500289 28.501260474955437 22.15258253614219 14-15 22.20329315361141 24.8708292798061 27.314141678530955 25.61173588805154 16-17 21.21430808404025 28.547632339792035 27.785418075582967 22.452641500584747 18-19 21.03894532225004 26.5316804199348 30.929650331666807 21.499723926148352 20-21 21.81128604516415 25.410621003281335 30.98601989982012 21.79207305173439 22-23 21.290735423593947 25.487645113564504 31.123058723151907 22.09856073968964 24-25 21.245815236547866 25.754543295377356 31.007716756753485 21.99192471132129 26-27 20.889243215931405 26.01015984049232 31.0040028198247 22.09659412375158 28-29 20.761434684452944 26.17403356716156 31.22469298696897 21.839838761416527 30-31 20.82529362892205 25.7500571291937 31.178616883303594 22.246032358580653 32-33 20.696803793924758 25.93524058555246 30.930504232453554 22.43745138806923 34-35 21.264754791666352 26.015491301841315 30.7212882081203 21.998465698372037 36-37 21.202842355760428 25.830218108595904 30.71145126860625 22.255488267037418 38-39 20.872322209213326 26.254072843775244 30.689048020983655 22.184556926027778 40-41 21.064254598257502 26.09934656340755 30.786090271055176 22.050308567279767 42-43 20.90539412056592 26.217822261488376 30.32241277064398 22.55437084730173 44-45 21.023218943075165 26.274560824916946 30.267680465710157 22.434539766297735 46-47 21.191410682781722 26.61314482670968 29.718087375967222 22.477357114541377 48-49 21.327845410043203 26.64164819284401 29.490122494684833 22.54038390242795 50-51 21.1017935434856 26.89634193404857 29.454309362415042 22.547555160050788 52-53 21.35502602093179 26.95550556899407 29.078958920532465 22.610509489541673 54-55 21.14568374988462 26.878642018088467 29.17246085799931 22.8032133740276 56-57 21.196789080331982 27.090886814627922 29.036829980684264 22.675494124355826 58-59 21.390210962040747 26.777161230412773 29.106530612862198 22.726097194684282 60-61 21.174961736005663 26.645502997598292 29.735660832793908 22.44387443360214 62-63 21.071737565266847 26.583344929605584 29.783764815498266 22.561152689629303 64-65 20.80532954236109 26.582982970872028 29.738211185384593 22.873476301382293 66-67 20.936370373731418 26.696613578958512 29.41331276373701 22.95370328357306 68-69 20.91003274423951 26.399872955375574 29.717250088855618 22.9728442115293 70-71 20.991779288647553 26.29750353558653 29.573233908354574 23.137483267411348 72-73 20.8390686023893 26.252053352371906 29.589450533493466 23.319427511745328 74-75 20.766858219871214 26.588482302631878 29.31792638487824 23.326733092618674 76-77 20.79757209360027 26.355523078575633 29.449863157942513 23.397041669881585 78-79 20.976914546453067 26.30915656927073 29.25735915967073 23.456569724605473 80-81 20.925634586875624 26.44238041932654 29.02641212597949 23.605572867818353 82-83 20.854112119988322 26.12129819960038 29.3291344680555 23.695455212355796 84-85 20.736926473701722 26.030910685660707 29.258687959350333 23.973474881287242 86-87 20.877570280031215 26.190087294241483 29.14753513245374 23.784807293273563 88-89 20.735846659285208 25.905589941855894 28.992642743482232 24.365920655376666 90-91 20.578287885479877 25.722557341739222 29.516853116813184 24.182301655967716 92-93 20.712574270605177 25.481019398090442 29.476959473359393 24.32944685794499 94-95 20.3449000860662 25.682517065762745 29.218037902404863 24.754544945766195 96-97 20.352884583385016 25.672776999573415 29.420209177298485 24.55412923974308 98-99 20.447015609540607 26.062244518204068 28.863429378855866 24.627310493399452 100-101 20.854633892542335 25.27300006654849 29.19240379205411 24.679962248855063 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 586.0 1 346.5 2 108.0 3 105.0 4 87.5 5 86.0 6 148.0 7 315.0 8 554.5 9 682.5 10 618.0 11 509.0 12 516.5 13 549.0 14 562.5 15 606.0 16 634.0 17 666.0 18 706.5 19 755.5 20 833.0 21 959.0 22 1083.0 23 1235.0 24 1457.0 25 1745.5 26 2114.0 27 2577.5 28 3040.5 29 3479.0 30 4012.0 31 4634.5 32 5341.0 33 5891.5 34 6468.0 35 7023.0 36 7740.5 37 8462.0 38 8898.5 39 9287.5 40 9545.5 41 10013.5 42 10604.5 43 11200.5 44 11593.0 45 11895.0 46 12195.0 47 12696.5 48 13690.0 49 13583.5 50 12624.0 51 11920.0 52 11316.0 53 11120.0 54 10848.5 55 9970.5 56 8915.5 57 7781.5 58 6904.0 59 6121.0 60 5056.0 61 4103.0 62 3292.0 63 2512.0 64 1810.0 65 1360.0 66 974.5 67 737.5 68 555.0 69 370.0 70 261.0 71 170.0 72 121.5 73 106.5 74 86.5 75 58.0 76 42.5 77 24.5 78 15.5 79 14.0 80 8.5 81 6.5 82 4.5 83 1.0 84 0.5 85 1.0 86 1.0 87 1.0 88 1.5 89 1.0 90 1.0 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3720949883361952 2 0.320490573895409 3 0.3096264866447172 4 0.3138514094644307 5 0.31656743127710363 6 0.27703422489264173 7 0.27733600509404976 8 0.2758271040870093 9 0.29031255375459836 10-11 0.29649904788346454 12-13 0.2824662685179876 14-15 0.2696406099581431 16-17 0.2663210277426539 18-19 0.25334447908210533 20-21 0.2599836435130837 22-23 0.25651317119689043 24-25 0.25726762170041073 26-27 0.25651317119689043 28-29 0.2791466863024984 30-31 0.2943865864736078 32-33 0.2848805101292525 34-35 0.2786940160003863 36-37 0.2614925445201242 38-39 0.2486668859602797 40-41 0.2435366225363419 42-43 0.25922919300956343 44-45 0.2598327534123796 46-47 0.2746199832813768 48-49 0.2850314002299565 50-51 0.2980079488905051 52-53 0.3186798926869604 54-55 0.28231537841728355 56-57 0.24368751263704594 58-59 0.23010740357368112 60-61 0.23206897488283382 62-63 0.24172594132789324 64-65 0.22844761246593653 66-67 0.23599211750113921 68-69 0.2337287659905784 70-71 0.24127327102578108 72-73 0.24444196314056618 74-75 0.24851599585957565 76-77 0.2645103465342053 78-79 0.2648121267356134 80-81 0.2525900285785851 82-83 0.2418768314285973 84-85 0.22241200843777445 86-87 0.229654733271569 88-89 0.24414018293915807 90-91 0.24293306213352567 92-93 0.24715798495323918 94-95 0.24368751263704594 96-97 0.2516846879743607 98-99 0.26073809401660397 100-101 0.2356903372997311 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 331367.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 74.89858801819545 #Duplication Level Percentage of deduplicated Percentage of total 1 88.04845087625253 65.94704647820758 2 7.557708564162456 11.321234002175828 3 1.897474813688569 4.26354553036087 4 0.8133147740739378 2.4366451276990233 5 0.4520948703219771 1.693063371869263 6 0.2687630271810853 1.2077982748415503 7 0.1864155346711315 0.9773582232067297 8 0.13925785507396182 0.8344173372385789 9 0.10227982629927362 0.6894553115305698 >10 0.496038042995569 6.36766185425865 >50 0.02493171102461003 1.2830501287334573 >100 0.011661606769462929 1.4011148354046403 >500 4.0212437136079066E-4 0.19727648692821143 >1k 0.001206373114082372 1.3803330375450273 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1971 0.5948087769753777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1540 0.46474151016848386 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1072 0.32350837590949005 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 655 0.1976660319223097 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 391 0.11799605875056961 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 373 0.11256401512522368 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 1.508901007040532E-4 0.0 0.0 0.0 0.0 20-21 3.017802014081064E-4 0.0 0.0 0.0 0.0 22-23 3.017802014081064E-4 0.0 0.0 0.0 0.0 24-25 6.035604028162128E-4 0.0 0.0 3.017802014081064E-4 0.0 26-27 6.035604028162128E-4 0.0 0.0 9.053406042243193E-4 0.0 28-29 6.035604028162128E-4 0.0 0.0 0.0022633515105607983 0.0 30-31 6.035604028162128E-4 0.0 0.0 0.005432043625345915 0.0 32-33 6.035604028162128E-4 0.0 0.0 0.009053406042243192 0.0 34-35 6.035604028162128E-4 0.0 0.0 0.01176942785491615 0.0 36-37 6.035604028162128E-4 0.0 0.0 0.01554168037251748 0.0 38-39 6.035604028162128E-4 0.0 0.0 0.025500427018984993 0.0 40-41 6.035604028162128E-4 0.0 0.0 0.04753038172177676 0.0 42-43 6.035604028162128E-4 0.0 0.0 0.08464934649497385 0.0 44-45 6.035604028162128E-4 0.0 0.0 0.1208629706639466 0.0 46-47 6.035604028162128E-4 0.0 0.0 0.20822833897159343 0.0 48-49 6.035604028162128E-4 0.0 0.0 0.2987623993940254 0.0 50-51 6.035604028162128E-4 0.0 0.0 0.3726985487390114 0.0 52-53 6.035604028162128E-4 0.0 0.0 0.47258779540509466 0.0 54-55 6.035604028162128E-4 0.0 0.0 0.5687047895535765 0.0 56-57 6.035604028162128E-4 0.0 0.0 0.7624476788575809 0.0 58-59 6.035604028162128E-4 0.0 0.0 0.9780696327636729 0.0 60-61 6.035604028162128E-4 0.0 0.0 1.2234169365084635 0.0 62-63 6.035604028162128E-4 0.0 0.0 1.4861166018342202 0.0 64-65 6.035604028162128E-4 0.0 0.0 1.78095585860994 0.0 66-67 6.035604028162128E-4 0.0 0.0 2.2128033268249405 0.0 68-69 6.035604028162128E-4 0.0 0.0 2.758874601272909 0.0 70-71 6.035604028162128E-4 0.0 0.0 3.1920800803942457 0.0 72-73 6.035604028162128E-4 0.0 0.0 3.6166848237754516 0.0 74-75 6.035604028162128E-4 0.0 0.0 4.106926760962921 0.0 76-77 6.035604028162128E-4 0.0 0.0 4.631118970808801 0.0 78-79 6.035604028162128E-4 0.0 0.0 5.258670899636959 0.0 80-81 6.035604028162128E-4 0.0 0.0 6.028059523126926 0.0 82-83 6.035604028162128E-4 0.0 0.0 6.769231697785235 0.0 84-85 6.035604028162128E-4 0.0 0.0 7.508291411033688 0.0 86-87 6.035604028162128E-4 0.0 0.0 8.225170279478645 0.0 88-89 6.035604028162128E-4 0.0 0.0 9.39954793325829 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 900 0.0 44.377865 1 GTATCAA 1730 0.0 42.60055 1 GAAGCGA 45 9.6177444E-4 42.219868 6 ATCAACG 2200 0.0 33.268284 3 TATCAAC 2215 0.0 33.257553 2 TCAACGC 2210 0.0 33.112736 4 CAACGCA 2250 0.0 32.730305 5 AACGCAG 2280 0.0 32.498188 6 CCGACGC 70 1.7071216E-8 30.529392 94-95 GTACATG 1455 0.0 30.391321 1 CCTTAGA 95 3.4595985E-5 30.030136 1 GTACTAT 65 0.005839717 29.229141 6 TACATGG 1485 0.0 29.123749 2 ACGCAGA 2575 0.0 28.95955 7 CGCAGAG 2605 0.0 27.896717 8 TAACCGA 35 0.008348049 27.17012 50-51 GCAGAGT 2655 0.0 27.013561 9 ACATGGG 1565 0.0 26.723951 3 CATGGGA 965 0.0 25.60605 4 GTATAAC 115 1.2742878E-4 24.796234 3 >>END_MODULE