FastQCFastQC Report
Thu 26 May 2016
SRR937359_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937359_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences331367
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19880.5999390403993156No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16580.5003515739346405No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11410.34433120980664944No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT7330.22120488763214202No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4020.12131564096605878No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC3910.11799605875056961No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGTA405.3714745E-447.5199289
ACTAGTA702.0451436E-433.9428068
GTGTAGG2250.031.665611
CTAGTGC650.005839102429.2297944
GTATCAA27350.028.1343461
GTACATG16850.026.4976331
GTATAGA1151.2793542E-424.7817821
CTACACC1751.4799843E-724.4277574
TACATGG17350.024.3651832
GTAAGGT1601.8534465E-623.7492074
ACATGGG17400.023.4762293
TATCAAC33300.023.1073362
CTAGGCC1452.3284629E-522.9302714
ATCAACG33450.022.861723
CTATAGA1252.2517086E-422.799241
TCAACGC33550.022.7935754
TAAGGTG1703.1386935E-622.3521965
CAACGCA34250.022.327725
AGTACTT17350.022.3220412-13
AGGTAAG1503.025594E-522.1659282
Produced by FastQC (version 0.11.5)