FastQCFastQC Report
Thu 26 May 2016
SRR937358_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937358_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279652
Sequences flagged as poor quality0
Sequence length101
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17450.6239898159140647No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13090.46808175875731267No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8970.3207557964899232No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN4280.1530473588602978No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3250.11621586829345043No Hit
GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT3120.11156723356171241No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTTG352.7826487E-454.292168
GGTATCA8550.043.385091
GTATCAA15100.040.9428831
CCGTTCA550.002574797334.5495579
TATCAAC18400.033.059242
GTATAGG902.3869125E-531.7044851
ATCAACG19050.031.6760983
TCAACGC19350.031.1849984
CAACGCA19500.030.9451125
AACGCAG19950.030.4798096
GTACATG13100.029.4053081
TAGGTGT650.005828109629.2394754
CCTATAC650.005828109629.2394753
TACATGG13850.029.1655312
GAGTACA10200.027.9745461
CTAAGGC856.383817E-427.94953
ATGGGAG3700.026.967495
ACATGGG13550.026.653
CGCAGAG22900.026.3459228
ACGCAGA22950.026.2885257
Produced by FastQC (version 0.11.5)