##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR937358_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 279652 Sequences flagged as poor quality 0 Sequence length 101 %GC 44 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.710854919685897 33.0 31.0 34.0 30.0 34.0 2 31.823230300516357 33.0 31.0 34.0 30.0 34.0 3 31.8475605395277 33.0 31.0 34.0 30.0 34.0 4 35.46254630755367 37.0 35.0 37.0 33.0 37.0 5 35.46627594295768 37.0 35.0 37.0 33.0 37.0 6 35.503708180166775 37.0 35.0 37.0 33.0 37.0 7 35.44795317036889 37.0 35.0 37.0 33.0 37.0 8 35.441942128073464 37.0 35.0 37.0 33.0 37.0 9 37.06881767339407 39.0 37.0 39.0 33.0 39.0 10-11 36.973390142033665 39.0 37.0 39.0 33.0 39.0 12-13 36.826126757541516 39.0 37.0 39.0 33.0 39.0 14-15 38.00609507530788 40.0 38.0 41.0 33.0 41.0 16-17 37.97981062177277 40.0 38.0 41.0 33.0 41.0 18-19 37.974647061347675 40.0 38.0 41.0 33.0 41.0 20-21 37.92902786320141 40.0 38.0 41.0 33.0 41.0 22-23 37.77974053466451 40.0 38.0 41.0 33.0 41.0 24-25 37.624370646374786 40.0 37.0 41.0 32.0 41.0 26-27 37.47522635275271 40.0 37.0 41.0 32.0 41.0 28-29 37.49004476992834 40.0 37.0 41.0 32.0 41.0 30-31 37.296212793042784 39.5 37.0 41.0 32.0 41.0 32-33 37.05946855377398 39.0 36.0 41.0 31.0 41.0 34-35 36.93056727647219 39.0 36.0 41.0 31.0 41.0 36-37 36.94619741678944 40.0 36.0 41.0 31.0 41.0 38-39 36.834344113398075 40.0 36.0 41.0 31.0 41.0 40-41 36.63140617624762 39.5 35.5 41.0 30.5 41.0 42-43 36.371384792527856 39.0 35.0 41.0 30.0 41.0 44-45 36.08810593165792 39.0 35.0 41.0 29.5 41.0 46-47 35.68297383891408 39.0 35.0 41.0 27.5 41.0 48-49 35.284997067784246 39.0 35.0 41.0 26.0 41.0 50-51 34.36287063922303 38.0 34.0 40.0 23.5 40.5 52-53 34.399836225022526 38.0 34.0 40.0 24.0 40.5 54-55 34.70431107233276 39.0 35.0 40.0 24.0 41.0 56-57 34.52341302762004 38.0 34.0 40.0 23.0 41.0 58-59 34.27620757226839 38.0 34.0 40.0 22.5 41.0 60-61 33.979533491625304 38.0 34.0 40.0 22.0 41.0 62-63 33.635736558293885 37.5 33.5 40.0 20.0 41.0 64-65 33.24510105416732 37.0 33.0 40.0 18.5 41.0 66-67 32.856786291533766 36.5 33.0 39.0 16.5 41.0 68-69 32.38693805157839 36.0 32.0 39.0 12.0 41.0 70-71 31.937415073019324 35.0 32.0 39.0 7.5 40.0 72-73 31.528923447713588 35.0 31.0 38.0 6.5 40.0 74-75 31.08447284482142 35.0 31.0 37.0 2.0 39.5 76-77 30.643176161801094 35.0 31.0 37.0 2.0 39.0 78-79 30.175890034757487 35.0 30.0 36.0 2.0 39.0 80-81 29.705855849412842 34.0 30.0 36.0 2.0 37.5 82-83 29.22685158697238 34.0 29.0 35.0 2.0 37.0 84-85 28.831510234148155 34.0 29.0 35.0 2.0 37.0 86-87 28.412886373063664 34.0 29.0 35.0 2.0 36.0 88-89 28.008717978058442 34.0 27.5 35.0 2.0 36.0 90-91 27.615699154663652 34.0 27.0 35.0 2.0 35.5 92-93 27.175139101454665 33.5 26.0 35.0 2.0 35.0 94-95 26.652872141089638 33.0 25.0 35.0 2.0 35.0 96-97 26.0756547423226 33.0 24.5 35.0 2.0 35.0 98-99 25.326046300401927 32.5 21.0 35.0 2.0 35.0 100-101 23.542359790024747 30.5 2.0 34.0 2.0 35.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.07249096374808417 1101 2 -0.07374510855604655 1101 3 -0.15735866114173902 1101 4 0.06643416572087801 1101 5 0.07597784940113428 1101 6 0.11216814686665799 1101 7 0.04470889021349933 1101 8 0.1795063941767765 1101 9 0.26783025171454256 1101 10-11 0.18396876919572236 1101 12-13 0.05541202702275427 1101 14-15 0.12903929740749476 1101 16-17 0.4001420390178865 1101 18-19 0.1281862727787484 1101 20-21 0.12910973198683706 1101 22-23 0.17569374806986815 1101 24-25 0.13113271154421113 1101 26-27 0.10416964538519835 1101 28-29 0.09432461126571212 1101 30-31 0.01549743243108992 1101 32-33 0.005346705497224491 1101 34-35 0.12718872234508893 1101 36-37 -0.07115453215776313 1101 38-39 0.06805706766819952 1101 40-41 0.04354480540464323 1101 42-43 0.1669253945517113 1101 44-45 0.36299386328323635 1101 46-47 0.17432902944400297 1101 48-49 0.40076101140807907 1101 50-51 0.24183255507627877 1101 52-53 0.4948094083881429 1101 54-55 0.668516666673014 1101 56-57 0.6796258384057197 1101 58-59 0.7526583532194806 1101 60-61 0.6626211142903529 1101 62-63 0.7697351710398479 1101 64-65 0.6041774789562879 1101 66-67 0.7257724520663231 1101 68-69 1.0934371927148874 1101 70-71 1.0776271691269237 1101 72-73 1.001484328144425 1101 74-75 1.1581826742116625 1101 76-77 1.1349789573496487 1101 78-79 0.894793352847497 1101 80-81 0.8001855710875567 1101 82-83 1.178578811057264 1101 84-85 1.0537632395044838 1101 86-87 1.0643647192136676 1101 88-89 0.718349784616322 1101 90-91 1.0702624270975747 1101 92-93 0.9529980572878394 1101 94-95 1.0740284337605637 1101 96-97 0.9878228560066837 1101 98-99 1.0864381465231858 1101 100-101 1.7811835178783895 1102 1 0.0778980986065072 1102 2 0.04572377642235992 1102 3 0.4124061764842395 1102 4 0.18043192607922265 1102 5 0.09146337171755903 1102 6 0.10368950344960126 1102 7 0.19767641540946812 1102 8 0.05885369860806122 1102 9 0.10062500455408951 1102 10-11 0.23516057850623184 1102 12-13 0.012858835533400281 1102 14-15 0.21996874869528682 1102 16-17 0.1899196745961902 1102 18-19 0.2706914719468756 1102 20-21 0.183980952591547 1102 22-23 0.18310056297948307 1102 24-25 0.30188939047557994 1102 26-27 0.544467197776811 1102 28-29 0.2853500471959549 1102 30-31 0.3680810190572288 1102 32-33 0.498499801001941 1102 34-35 0.4014328432857397 1102 36-37 0.5900356774087072 1102 38-39 0.542285191168439 1102 40-41 0.8829944934545537 1102 42-43 0.996536656749754 1102 44-45 0.8148735625313535 1102 46-47 1.246125542001991 1102 48-49 1.2691980614800684 1102 50-51 1.184993649301198 1102 52-53 1.29388155684439 1102 54-55 1.3725640190966288 1102 56-57 1.2059256304389905 1102 58-59 1.394283693165086 1102 60-61 1.4140848800878203 1102 62-63 1.4821171899168277 1102 64-65 1.5050893330596367 1102 66-67 1.6477849300698466 1102 68-69 1.7044114368358265 1102 70-71 1.7052547487141858 1102 72-73 1.4762403671781748 1102 74-75 1.8123660048787507 1102 76-77 1.6972729903043735 1102 78-79 1.5919778033354177 1102 80-81 1.5351399783823858 1102 82-83 1.5786268033784943 1102 84-85 1.6635696704755247 1102 86-87 1.651630756647677 1102 88-89 1.6301398981981485 1102 90-91 1.7352920223623336 1102 92-93 1.4834091915063645 1102 94-95 1.4567352006778513 1102 96-97 1.4902064746277048 1102 98-99 1.5101303557697037 1102 100-101 2.1901900768289586 1103 1 -0.39989253181570206 1103 2 -0.5032351593786828 1103 3 -0.340326617038027 1103 4 -0.39649115084385755 1103 5 -0.17580094291517412 1103 6 -0.13452622818717686 1103 7 -0.34068390847717467 1103 8 -0.2451081290379733 1103 9 -0.2941841336761044 1103 10-11 0.005323967748573466 1103 12-13 -0.1635075612277319 1103 14-15 -0.3159118181063292 1103 16-17 -0.45101439133787125 1103 18-19 -0.5216812868732461 1103 20-21 -0.4840352417404361 1103 22-23 -0.8325660072923569 1103 24-25 -0.6261687356088643 1103 26-27 -0.4623864748663351 1103 28-29 -0.31730322058889016 1103 30-31 -0.4320346547426581 1103 32-33 -0.12323241415132458 1103 34-35 -0.539913310560415 1103 36-37 -0.08257132085618935 1103 38-39 -0.09133973653543137 1103 40-41 -0.02844998312149727 1103 42-43 0.1396685248816283 1103 44-45 0.3261517580200817 1103 46-47 0.40227071262084735 1103 48-49 0.5820233940422384 1103 50-51 0.45606074010513 1103 52-53 0.4187369645945367 1103 54-55 0.21160392655758642 1103 56-57 0.44046871892944495 1103 58-59 0.740799019943573 1103 60-61 0.41442467187498977 1103 62-63 0.19976467401168208 1103 64-65 0.35855231744367444 1103 66-67 0.5100174344076152 1103 68-69 0.6968203437184144 1103 70-71 0.5182174439137235 1103 72-73 0.6778453411515706 1103 74-75 0.5488827775797382 1103 76-77 0.7056665704084786 1103 78-79 0.9328468027570516 1103 80-81 0.7845037724830242 1103 82-83 0.5562222342633945 1103 84-85 0.25059974566349297 1103 86-87 0.4453988306789114 1103 88-89 0.38099299247229723 1103 90-91 0.3674854867926385 1103 92-93 0.30672469179554795 1103 94-95 0.4646154782949736 1103 96-97 0.317780216675132 1103 98-99 0.5843567872329771 1103 100-101 0.9491114186450353 1104 1 -0.09813322056734108 1104 2 -0.16678559920649505 1104 3 -0.20846717477712318 1104 4 -0.11299873085508239 1104 5 -0.13438226427022215 1104 6 -0.1083003251364616 1104 7 0.148399939548284 1104 8 0.1008491983062143 1104 9 0.17970225973584064 1104 10-11 -0.059206726617887284 1104 12-13 0.16665573654505295 1104 14-15 -0.3268490256676202 1104 16-17 -0.007438684805968876 1104 18-19 0.4026472738604241 1104 20-21 0.11553428848602465 1104 22-23 0.12919938668107278 1104 24-25 -0.02246529185067203 1104 26-27 -0.15318752296664684 1104 28-29 -0.21761905439011997 1104 30-31 -0.005251948397905437 1104 32-33 0.006044209468363704 1104 34-35 0.16834281895484082 1104 36-37 0.23031703774384482 1104 38-39 0.03762573230229549 1104 40-41 -0.05240422231296549 1104 42-43 -0.040759137480350205 1104 44-45 0.17603010351886184 1104 46-47 0.05757204146232198 1104 48-49 0.09579374777609928 1104 50-51 0.038631393296221006 1104 52-53 -0.1670060636667401 1104 54-55 0.006868759048693107 1104 56-57 -0.09544275402581803 1104 58-59 0.07436162522150624 1104 60-61 0.2885941459532191 1104 62-63 -0.10736381185767385 1104 64-65 -0.0834769732170102 1104 66-67 -0.20832432116042554 1104 68-69 -0.27065579328326805 1104 70-71 0.24773503702620658 1104 72-73 0.4128958745597693 1104 74-75 0.35789222919867925 1104 76-77 0.26232247533080866 1104 78-79 -0.12420681880387363 1104 80-81 0.2690349347514136 1104 82-83 -0.007868632290971789 1104 84-85 0.3992007188995075 1104 86-87 0.35902881383444196 1104 88-89 0.6190848884109066 1104 90-91 0.17606914765732284 1104 92-93 0.734688850507883 1104 94-95 0.6418988397024243 1104 96-97 0.48476037763339264 1104 98-99 0.5743694205850289 1104 100-101 1.224644826842681 1105 1 -0.03744909290167442 1105 2 -0.1229184172972353 1105 3 -0.06181982970770861 1105 4 0.1061672201968662 1105 5 0.12046602541061446 1105 6 -0.2991189883681784 1105 7 0.11338848971287518 1105 8 0.05308191621089264 1105 9 -0.3789692015494097 1105 10-11 -0.22988530826952314 1105 12-13 0.05368700890801392 1105 14-15 -0.05657923892081129 1105 16-17 -0.014702174143302216 1105 18-19 -0.09266544976917146 1105 20-21 -0.01570944988522882 1105 22-23 -0.1285784507312755 1105 24-25 -0.07588593017899825 1105 26-27 -0.0357462033731224 1105 28-29 0.4590259649314774 1105 30-31 -0.14651429275170358 1105 32-33 -0.14212381686625974 1105 34-35 0.26503578446221354 1105 36-37 0.06954971986779412 1105 38-39 0.35181637206185457 1105 40-41 0.28897083135813517 1105 42-43 0.35494001809429676 1105 44-45 0.4349752874318469 1105 46-47 0.554222070086908 1105 48-49 0.41980619947210585 1105 50-51 0.606400106620967 1105 52-53 0.6763727112008695 1105 54-55 0.6624523428471818 1105 56-57 0.3666565017348731 1105 58-59 0.5920999249209444 1105 60-61 0.45953213793833214 1105 62-63 0.48932915364969176 1105 64-65 0.24644824504547813 1105 66-67 0.512930330335216 1105 68-69 0.4565205699627626 1105 70-71 0.8496654077146282 1105 72-73 0.7636767891153724 1105 74-75 0.8405117368557562 1105 76-77 1.032815891675451 1105 78-79 1.0494192009470957 1105 80-81 0.9074392928450159 1105 82-83 1.046182641503215 1105 84-85 0.8550115103693798 1105 86-87 1.3285436270590019 1105 88-89 1.3344714992596245 1105 90-91 1.3425703284216013 1105 92-93 1.4484384927005252 1105 94-95 1.3275114058967823 1105 96-97 1.688849221200016 1105 98-99 1.8261271492239288 1105 100-101 1.196735853034177 1106 1 -0.04131542816817557 1106 2 -0.26887257360764494 1106 3 -0.5844309868466198 1106 4 -0.2411643180522347 1106 5 -0.22567454349299965 1106 6 -0.011422219519793941 1106 7 -0.24497246030093578 1106 8 -0.24317602358474488 1106 9 -0.53346726838177 1106 10-11 -1.0898773684738856 1106 12-13 -1.2207710137376608 1106 14-15 -0.9567794598542534 1106 16-17 -0.8904961537069624 1106 18-19 -0.3709725472560592 1106 20-21 -0.6972891240084067 1106 22-23 -0.6404795139734674 1106 24-25 -0.8089016716868684 1106 26-27 -0.3622961895647805 1106 28-29 -0.3199801797799324 1106 30-31 -0.21141349186797243 1106 32-33 -0.4909915329667811 1106 34-35 -0.3304920393433619 1106 36-37 -0.1130742099858395 1106 38-39 -0.6758693501143469 1106 40-41 -0.26344908026847946 1106 42-43 0.1511979578899343 1106 44-45 -0.056946833529217145 1106 46-47 0.2584733128375376 1106 48-49 0.300053368762363 1106 50-51 0.5450910759664467 1106 52-53 0.4621190519907046 1106 54-55 0.5564215502484515 1106 56-57 0.7891054108759974 1106 58-59 0.3826101430927267 1106 60-61 0.4107455458367042 1106 62-63 0.36093748007885296 1106 64-65 0.5466933430846908 1106 66-67 0.49564401440039063 1106 68-69 0.3533118569000706 1106 70-71 0.2772234027436262 1106 72-73 0.6703065184719001 1106 74-75 0.3883058545028142 1106 76-77 0.5644197303940359 1106 78-79 0.6358939316844605 1106 80-81 0.6772628912984828 1106 82-83 -0.12292637534295636 1106 84-85 -0.47134861114401616 1106 86-87 -0.5442815593535926 1106 88-89 0.3206372683841785 1106 90-91 0.31711983132134947 1106 92-93 0.5767093432942865 1106 94-95 0.25304993116604635 1106 96-97 0.4552034741688118 1106 98-99 0.6111399219386477 1106 100-101 0.024960100479635372 1107 1 -0.15420488612805627 1107 2 -0.46100516714869144 1107 3 -0.23836469007610006 1107 4 -0.25795191230461967 1107 5 -0.15233551944974977 1107 6 -0.8034267720877253 1107 7 -0.7769115697048363 1107 8 -0.5644165983464404 1107 9 -0.13390829840026441 1107 10-11 -0.06409133916672971 1107 12-13 -0.16151649673666668 1107 14-15 -0.530509720204229 1107 16-17 -0.22667484199832444 1107 18-19 -0.6363374640294239 1107 20-21 -0.7903677980729924 1107 22-23 -0.2978632100895524 1107 24-25 -0.11584433778446623 1107 26-27 -0.029247391727253103 1107 28-29 -0.3777053184909889 1107 30-31 -0.17993675264475684 1107 32-33 0.11396649804758141 1107 34-35 0.13931163116452439 1107 36-37 0.0365914608065907 1107 38-39 0.09052700783131229 1107 40-41 -0.15617919835071348 1107 42-43 -0.5730160128029098 1107 44-45 0.09584872771704767 1107 46-47 0.08387521922535512 1107 48-49 0.5654538524727002 1107 50-51 0.4837025427469328 1107 52-53 0.9516623725199409 1107 54-55 0.3197235846772415 1107 56-57 0.5287554322161654 1107 58-59 0.5685185226630765 1107 60-61 0.5258757208260292 1107 62-63 0.6413827781297883 1107 64-65 0.920430716822068 1107 66-67 0.8121541865443618 1107 68-69 0.4469951688932383 1107 70-71 0.9054066436029196 1107 72-73 0.7773985657047966 1107 74-75 1.0600230262199837 1107 76-77 1.0863289635708746 1107 78-79 1.0429575253677719 1107 80-81 0.4536179915972447 1107 82-83 0.7623702529114169 1107 84-85 0.7447916648554127 1107 86-87 1.3585495688296483 1107 88-89 0.9224654089447135 1107 90-91 1.3223126041197588 1107 92-93 1.4172627648336196 1107 94-95 1.0349534736329709 1107 96-97 1.074637248532163 1107 98-99 1.0449142852904743 1107 100-101 1.3005682951019146 1108 1 -0.18702047924515952 1108 2 -0.1913488045275038 1108 3 -0.13575568545704542 1108 4 -0.4145429482926346 1108 5 -0.39508969436472796 1108 6 -0.08828540870515411 1108 7 -0.18217018601872326 1108 8 -0.24199641662978166 1108 9 -0.08302255655975443 1108 10-11 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-2.934508604547851 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1238.0 3 239.0 4 213.0 5 240.0 6 280.0 7 269.0 8 355.0 9 469.0 10 543.0 11 807.0 12 984.0 13 1400.0 14 1511.0 15 2030.0 16 2703.0 17 3535.0 18 3061.0 19 1975.0 20 1994.0 21 2040.0 22 2231.0 23 2320.0 24 2561.0 25 2764.0 26 3317.0 27 3816.0 28 4230.0 29 5115.0 30 6322.0 31 7617.0 32 9807.0 33 12801.0 34 17848.0 35 25240.0 36 38232.0 37 54987.0 38 47485.0 39 7071.0 40 2.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.76228181318938 18.894986773293898 15.372770573181194 22.96996084033553 2 14.757138757353996 19.812742143779598 41.05574687903573 24.37437221983068 3 18.534952793640773 24.457629064194908 30.81147588027664 26.195942261887676 4 12.03077916487301 16.150093270196585 37.99074472664658 33.82838283828383 5 13.03196736896105 37.034579000046634 34.85487151707785 15.078582113914468 6 30.896832537017062 36.164706641385266 17.965683726831855 14.972777094765819 7 27.440678634273613 33.1378950725905 21.12919353046794 18.292232762667947 8 26.09409327613334 33.91608516921665 20.243995971629374 19.745825583020633 9 25.69310053183102 16.39179174013389 20.67080950128299 37.24429822675211 10-11 24.302061957291226 26.36760370274121 27.715000044805677 21.615334295161883 12-13 25.591276454670897 23.77685787502464 28.397218787520384 22.23464688278408 14-15 22.163406832659863 24.933743089838813 27.330101871679314 25.572748205822005 16-17 21.07479557953627 28.50543239654434 27.86694884426055 22.552823179658844 18-19 20.915056102464494 26.54232501800798 30.990205810573997 21.55241306895353 20-21 21.613311856432052 25.450486365810875 31.247099632873994 21.68910214488308 22-23 21.13383682282008 25.471192696714777 31.30772883238817 22.087241648076976 24-25 21.145739086812803 25.597011582804058 31.250100778457597 22.00714855192554 26-27 20.81360329225185 25.93293308895852 31.113535748774098 22.139927870015534 28-29 20.536874266412205 26.202187995591753 31.42345151376681 21.83748622422923 30-31 20.561457840480593 25.818493985404388 31.286650321877644 22.333397852237372 32-33 20.48876997489306 26.06337128346472 31.074186443685004 22.373672297957217 34-35 21.074713426480184 26.22533030331271 30.660499093144463 22.039457177062648 36-37 20.989215947998616 25.877424799198767 30.980275522764046 22.153083730038574 38-39 20.859401314327894 26.162936526705792 30.81665340872444 22.161008750241866 40-41 20.970784729301446 26.06316747496717 30.945039746169158 22.021008049562226 42-43 20.708696828476878 26.158391609018082 30.645932885761738 22.4869786767433 44-45 20.76526525719385 26.42430041517271 30.338069205007873 22.47236512262557 46-47 21.12118784842246 26.62910737549077 29.785270521869865 22.464434254216908 48-49 21.215091783318606 26.758470893317014 29.487508042129107 22.53892928123527 50-51 21.09360718318189 26.934243776547127 29.452658745093824 22.51949029517716 52-53 21.346159362092703 26.831760555300598 29.259766521340065 22.562313561266638 54-55 20.93943879544241 26.91384087137599 29.41897515116108 22.727745182020524 56-57 21.03240647018673 27.083202703017427 29.23121297190388 22.65317785489196 58-59 21.33940759796633 26.741752985478374 29.226337419070514 22.692501997484783 60-61 21.07113042668224 26.570455058454286 29.893553368421426 22.464861146442043 62-63 20.93682814963451 26.46302135588362 30.030636376666187 22.569514117815682 64-65 20.829675861401817 26.599643866733068 29.81111322099968 22.75956705086543 66-67 20.76540126868225 26.66579484322405 29.529376568610417 23.039427319483284 68-69 20.813121144159187 26.385057430084334 29.740579388224504 23.061242037531976 70-71 20.957660080297686 26.34505789423709 29.60694981663391 23.090332208831313 72-73 20.803078593349422 26.228239429428303 29.715484514768615 23.253197462453667 74-75 20.638995838088565 26.553460764623573 29.367185218495873 23.44035817879199 76-77 20.668141870403055 26.449208496456823 29.40487175582093 23.477777877319188 78-79 20.741705575243756 26.187771658311625 29.444983928936157 23.62553883750847 80-81 20.725995735760485 26.297995090749467 29.222224212996974 23.753784960493075 82-83 20.654078360414914 25.95119941238646 29.668929934251775 23.725792292946846 84-85 20.55900487602151 25.942333253320243 29.57265845278571 23.926003417872536 86-87 20.727812103091786 26.01621938264594 29.4524928299007 23.80347568436157 88-89 20.45857622454793 26.00025796928016 29.03139701113926 24.509768795032656 90-91 20.459777528158327 25.66058084893306 29.61268974511943 24.266951877789182 92-93 20.361011735747724 25.48489902527548 29.72524627872356 24.428842960253238 94-95 20.110350757765755 25.5773709290244 29.387338325391422 24.924939987818423 96-97 19.968650352907456 25.69166278528179 29.54480312421626 24.794883737594496 98-99 20.301744679783713 26.193666980797413 29.148944556520245 24.355643782898625 100-101 20.289800553877683 26.10515794112273 28.887802151739557 24.71723935326003 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 424.0 1 248.0 2 79.0 3 80.0 4 69.5 5 77.0 6 145.5 7 296.0 8 484.0 9 589.0 10 512.5 11 438.5 12 458.5 13 490.0 14 532.0 15 557.5 16 586.5 17 619.5 18 647.5 19 704.0 20 753.0 21 832.5 22 948.0 23 1091.5 24 1292.5 25 1513.0 26 1783.0 27 2169.5 28 2573.0 29 2942.0 30 3387.0 31 3863.5 32 4454.0 33 4994.5 34 5484.5 35 5967.0 36 6479.0 37 7019.5 38 7439.5 39 7766.5 40 8103.5 41 8634.5 42 9064.0 43 9452.0 44 9837.0 45 10163.5 46 10241.0 47 10551.5 48 11539.0 49 11541.5 50 10691.0 51 10104.0 52 9597.5 53 9435.5 54 9200.0 55 8411.5 56 7468.5 57 6581.5 58 5824.5 59 4992.5 60 4196.0 61 3427.0 62 2625.5 63 1957.0 64 1438.0 65 1093.0 66 791.0 67 621.0 68 451.5 69 278.5 70 195.0 71 154.5 72 120.5 73 75.0 74 54.0 75 46.5 76 35.5 77 21.0 78 15.0 79 12.0 80 6.0 81 3.5 82 2.5 83 2.5 84 2.0 85 1.0 86 1.0 87 0.5 88 1.0 89 1.0 90 0.0 91 1.5 92 2.0 93 1.0 94 1.5 95 1.0 96 1.0 97 1.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3751090641225523 2 0.31896786005463934 3 0.313246463461731 4 0.31896786005463934 5 0.3214709710640367 6 0.23565002217041178 7 0.2220617052622545 8 0.22563757813282223 9 0.22027376882697067 10-11 0.2394046886845079 12-13 0.22778310185516282 14-15 0.2233132607669532 16-17 0.22581637177635064 18-19 0.21777065781757327 20-21 0.21187046758113656 22-23 0.21437357859053394 24-25 0.2054338964141147 26-27 0.20471872184000114 28-29 0.2251011972022371 30-31 0.23743795860569566 32-33 0.23082259379514541 34-35 0.23815313317980918 36-37 0.20829459471056888 38-39 0.20525510277058628 40-41 0.20328837269177405 42-43 0.21115529300702301 44-45 0.21866462603521522 46-47 0.22402843534106676 48-49 0.2333257048045428 50-51 0.23851072046686597 52-53 0.26461459242201024 54-55 0.230643800151617 56-57 0.19899732524709282 58-59 0.1970305951682806 60-61 0.19488507144593997 62-63 0.20453992819647274 64-65 0.19416989687182643 66-67 0.1943486905153548 68-69 0.18952126214008838 70-71 0.20221561083060374 72-73 0.2015004362564902 74-75 0.20507630912705793 76-77 0.2125856421552501 78-79 0.20704303920587014 80-81 0.20990373750232433 82-83 0.19917611889062123 84-85 0.18987884942714517 86-87 0.19363351594124126 88-89 0.19631542059416704 90-91 0.20042767439531992 92-93 0.1957790396635819 94-95 0.19381230958476964 96-97 0.19381230958476964 98-99 0.20561269005764307 100-101 0.18916367485303162 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 279652.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 76.07661818011697 #Duplication Level Percentage of deduplicated Percentage of total 1 88.55082004513402 67.36646926109908 2 7.28963940303088 11.091422270702319 3 1.8400879083951251 4.199629956744778 4 0.7679019226661533 2.336775254818026 5 0.4082273506151086 1.5528278141713177 6 0.2627667191409771 1.1994242017518086 7 0.18652768458332286 0.9933276808046719 8 0.12650312167249522 0.7699143748857039 9 0.08994185033435209 0.6158224625669757 >10 0.4427641746191171 5.890186433113402 >50 0.02399744338151303 1.2958495101004461 >100 0.009410762110397038 1.2740133719467968 >500 4.7053810551985186E-4 0.32109862170167935 >1k 9.410762110397037E-4 1.0932387855930086 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1745 0.6239898159140647 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1309 0.46808175875731267 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 897 0.3207557964899232 No Hit NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN 428 0.1530473588602978 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 325 0.11621586829345043 No Hit GCTTACTCTGCGTTGATACCACTGCTTACTCTGCGTTGATACCACTGCTT 312 0.11156723356171241 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.787936435283853E-4 0.0 20-21 0.0 0.0 0.0 3.575872870567706E-4 0.0 22-23 0.0 0.0 0.0 3.575872870567706E-4 0.0 24-25 0.0 0.0 0.0 7.151745741135412E-4 0.0 26-27 0.0 0.0 0.0 7.151745741135412E-4 0.0 28-29 0.0 0.0 0.0 0.0021455237223406234 0.0 30-31 0.0 0.0 0.0 0.006972952097607026 0.0 32-33 0.0 0.0 0.0 0.009118475819947649 0.0 34-35 0.0 0.0 0.0 0.013588316908157282 0.0 36-37 0.0 0.0 0.0 0.017164189778724987 0.0 38-39 0.0 0.0 0.0 0.028070602033956487 0.0 40-41 0.0 0.0 0.0 0.04916825197030595 0.0 42-43 0.0 0.0 0.0 0.07759644129131921 0.0 44-45 0.0 0.0 0.0 0.10423669417704862 0.0 46-47 0.0 0.0 0.0 0.18558780198246394 0.0 48-49 0.0 0.0 0.0 0.27784532204311074 0.0 50-51 0.0 0.0 0.0 0.3549053824038448 0.0 52-53 0.0 0.0 0.0 0.454314648205627 0.0 54-55 0.0 0.0 0.0 0.5526511521462389 0.0 56-57 0.0 0.0 0.0 0.7750704446955502 0.0 58-59 0.0 0.0 0.0 0.9837226266931758 0.0 60-61 0.0 0.0 0.0 1.254416202995151 0.0 62-63 0.0 0.0 0.0 1.5403072389970394 0.0 64-65 0.0 0.0 0.0 1.8346015762447614 0.0 66-67 0.0 0.0 0.0 2.2764006694034014 0.0 68-69 0.0 0.0 0.0 2.843891693962496 0.0 70-71 0.0 0.0 0.0 3.3087551671362982 0.0 72-73 0.0 0.0 0.0 3.7537725458784488 0.0 74-75 0.0 0.0 0.0 4.280141032426015 0.0 76-77 0.0 0.0 0.0 4.871232817930856 0.0 78-79 0.0 0.0 0.0 5.538669489222319 0.0 80-81 0.0 0.0 0.0 6.441219801753608 0.0 82-83 0.0 0.0 0.0 7.266352466637106 0.0 84-85 0.0 0.0 0.0 8.074678529028937 0.0 86-87 0.0 0.0 0.0 8.879607512193726 0.0 88-89 0.0 0.0 0.0 10.253994249996424 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position ACCGTTG 35 2.7826487E-4 54.29216 8 GGTATCA 855 0.0 43.38509 1 GTATCAA 1510 0.0 40.942883 1 CCGTTCA 55 0.0025747973 34.549557 9 TATCAAC 1840 0.0 33.05924 2 GTATAGG 90 2.3869125E-5 31.704485 1 ATCAACG 1905 0.0 31.676098 3 TCAACGC 1935 0.0 31.184998 4 CAACGCA 1950 0.0 30.945112 5 AACGCAG 1995 0.0 30.479809 6 GTACATG 1310 0.0 29.405308 1 TAGGTGT 65 0.0058281096 29.239475 4 CCTATAC 65 0.0058281096 29.239475 3 TACATGG 1385 0.0 29.165531 2 GAGTACA 1020 0.0 27.974546 1 CTAAGGC 85 6.383817E-4 27.9495 3 ATGGGAG 370 0.0 26.96749 5 ACATGGG 1355 0.0 26.65 3 CGCAGAG 2290 0.0 26.345922 8 ACGCAGA 2295 0.0 26.288525 7 >>END_MODULE