FastQCFastQC Report
Thu 26 May 2016
SRR937358_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR937358_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences279652
Sequences flagged as poor quality0
Sequence length101
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17350.6204139430434968No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14550.5202895026676012No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9950.3557993506214867No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT6150.2199161815399139No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC3470.12408278860869938No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3320.11871897930284783No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAGGTG1203.759851E-935.6368065
GACGACC550.002576635734.5445447
ACAGTCG600.00393948931.677165
GACCGCG600.00394639431.6658347
TCGCGGG300.003949742331.66583486-87
GTAAGGT953.4736353E-530.0099414
GGTACTC804.4763472E-429.6973363
CTACCCC804.4763472E-429.6973364
GTATCAA24250.029.5977081
GTGCTAC650.00581698429.250921
GGTATCA16550.029.0078961
CCTTAAG700.00836736927.151852
CCTACGT700.00836736927.151852
CTACGTC700.00836736927.151853
ACGTCTA700.00836736927.151855
GTCTATC700.00838198427.1421417
CGTCTAT1056.88871E-527.1421416
GGGCGTA700.00838198427.1421419
GTACAAC908.8946865E-426.407081
GAGTACT15300.025.76729612-13
Produced by FastQC (version 0.11.5)